Marine debris and associated organic pollutants in surface waters of Chiloé in the Northern Chilean Patagonia (42°-44°S).

We report the occurrence of plastics and associated persistent organic pollutants (POPs) in surface waters from Northern Chilean Patagonia. A total of 200 particles were found during the conducted survey. The highest number of particles found was 0.6 item m-3. We found that 53 % of the collected particles corresponded to plastic, with an average of 0.19 ± 0.18 item m-3. Microplastics (68 %) were the dominant size found in the area, followed by macroplastics (18 %) and mesoplastics (14 %). Most plastic particles were white (55 %) while others were <10 % each. Black and light blue represented 9 %; red, dark blue, and other colors 7 %; and green 6 %. Fragments were the most frequent shape of plastic debris (38 %), followed by Styrofoam (30 %) and fiber (27 %). Higher PBDE levels were found in the central zone, and those were higher than DDT, PeCB, HCB, and PCB levels. This study is the first report on POP occurrence in marine plastic debris from Chiloé Sea in the Northern Chilean Patagonia.

Occurrence, source estimation, and risk assessment of Polycyclic Aromatic Hydrocarbons in coastal seawaters from the Quintero Industrial Complex (Valparaíso, Chile).

In the 1960s, the Quintero industrial complex was inaugurated in Chile. This began a history of dramatic anthropogenic impacts on the Chilean coast. Among the known, we could mention high atmospheric emissions of chemicals due to combustion processes and frequent oil spills. For this reason, we surveyed the concentrations of fifteen EPAPAHs in the surface coastal waters of the Quintero Bay area in 2015. The levels found are in the range of the highest levels when reviewing the literature (0.97 µg L-1 up to 9.84 µg L-1). The highest levels were found in the vicinity of the industrial complex and decreased in the other two zones. The concentration of individual compounds significantly exceeds the levels recommended by the EPA (Environmental Protection Agency) and the EU water framework directive (WFD). The risk estimations revealed that PAH concentrations represent high-risk for wildlife. Molecular ratios of PAHs were used to identify the possible sources, being these were mainly of pyrogenic origin, agreeing with an origin in the combustion of wood, coal, grass, and fossil fuels. This study contributes to the first data for surface water in a country's highly impacted industrial coastal area.

A decade later, reviewing floating marine debris in Northern Chilean Patagonia.

Floating marine debris (FMD) were abundantly reported in the Northern Chilean Patagonia in 2009 where sea-based activities (mussel and salmon aquaculture) are responsible for most of them. Identifying the sources of FMD is important to take the necessary actions to diminish their abundance. In 2019 eleven transects were opportunistically conducted to evaluate the abundance, spatial distribution and composition of FMD in the same area, and to compare them with the previous study using the same methodology. FMD identified were classified into the same six categories from the previous study, however "Others" category were now the dominant one indicating that new pollutants have been increasing during the last decade. Inside the "others" category, plastics buoy and other floating devices, that normally are used by the salmon aquaculture, were the main items. Similarly than the previous study, styrofoam and domestic plastics were also the other most abundant items. In general, also, an increase in abundance compared with the previous study was found. The highest abundances were similarly found in the "Golfo de Corcovado" zone ranging from 50 to 230 items km-2. Our results, as well as the previous study, confirm that sea-based activities are still responsible for the FMD found in the Northern Patagonia. To avoid the origin of this contamination it is urgent to implement public policies that effectively reduce FMD, and hold the aquaculture industries responsible.

Microplastic ingestion cause intestinal lesions in the intertidal fish Girella laevifrons.

We exposed juvenile intertidal fish to different amounts of Poly(styrene-co-divinylbenzene) microplastics in their diet. We fed ten individuals with pellets containing 0.01 g, another ten fish with pellets containing 0.1 g of PS, and ten fish without plastic as control. After 45 days of treatment, the whole intestine was removed, and the histological evaluation started immediately. We evaluated inflammation due to leukocyte infiltration (Lk), circulatory disorders like Hypermeia (Hyp), and regressive changes in the intestinal tissue, assessing Crypt cell loss (Ccl) and Villi cell loss (Vcl). The severity of the lesions increased according to the microplastic concentration. In the fish group feeding on microplastics, we found that leukocyte infiltration and hyperemia were more severe in the higher exposure group compared to the lower exposure; and crypt cell loss and villi cell loss increased significantly due to Poly(styrene-co-divinylbenzene) microplastic physical abrasion.

First detection of plastic microfibers in a wild population of South American fur seals (Arctocephalus australis) in the Chilean Northern Patagonia.

The dramatic increase of microplastics (plastic fragments <5 mm) in marine environments is a problem that has attracted public attention globally. Within the different types of microplastics, microfibres are the least studied (size <1 mm). We examined 51 female scats from a population in Northern Patagonia. Our results showed no presence of microplastic particles, however 67% of them showed a remarkable abundance of microfibers, which until now had only been reported in animals fed in captivity. As a result of this work we propose that the examination of scats from South American Fur Seal and also other pinnipeds could be an efficient tool to monitor environmental levels of microfibres and maybe microplastics in the environment due to the easy recognition of the animals and their scats.

Coastal debris survey in a Remote Island of the Chilean Northern Patagonia.

Global marine litter pollution is increasing dramatically, and oceanic islands are one of the most vulnerable ecosystems due to their high debris accumulation rate compared to continental sites. Remote areas, such as inhabited islands, represent a perfect study case to track marine debris sources, due to the assumed low rates of local production of debris. Guafo Island is one of the largest islands of the Chilean Northern Patagonia and is considered a remote zone. The accessible coast of Guafo Island was monitored during four austral summers revealing higher levels of marine debris accumulation than continental Chile. Plastic was the most abundant type of debris constituting 50% of the total litter monitored. Our results suggest that most of the plastic identified is likely to be related to local fisheries activities. Mitigation measures including collaboration among fishing communities and scientists could contribute to reduce the coastal debris pollution in remote areas.