RESUMO
The paper covers a study of four rabies viruses, isolated in 2003 from man, wild foxes, badgers and home cats inhabiting entirely different districts of Novosibirsk suburb, and a sample taken from a bat from Maslaninsky district. The samples were studied by fluorescent antibody microscopy and a biological test on white mice, RT-PCR-analysis of NP-genes and sequencing. The study revealed that related variants of rabies viruses circulated in Novosibirsk region, and those formed two groups in genotype 1. Most of the amino acid replacements found in the study are only characteristic of the viruses circulating in the given territory.
Assuntos
RNA Viral/genética , Raiva/epidemiologia , Rhabdoviridae/genética , Animais , Anticorpos Antivirais/imunologia , Gatos , Quirópteros/virologia , Doenças Endêmicas , Raposas/virologia , Genótipo , Humanos , Masculino , Camundongos , Microscopia de Fluorescência , Mustelidae/virologia , Reação em Cadeia da Polimerase , Raiva/virologia , Rhabdoviridae/imunologia , Rhabdoviridae/isolamento & purificação , Sibéria/epidemiologiaRESUMO
We caught, 2002-2003 in caves of Novosibirsk Region and Altai Territory, 52 bats (Myotis daubentoni, Myotis brandtii, Murina leucogaster, Plecotus auritus). Examinations of the animals' brain samples by fluorescence antibody microscopy and RT-PCR revealed lyssaviruses, genotype 1, which were close to strains provoking rabies in the local animals. According to the results, bats can be involved in keeping alive the rabies foci in Western Siberia, which must be taken into account in antiepizootic measures.
Assuntos
Encéfalo/virologia , Quirópteros/virologia , Reservatórios de Doenças/veterinária , Vírus Lassa/isolamento & purificação , Sequência de Aminoácidos , Animais , Antígenos Virais/análise , Feminino , Febre Lassa/epidemiologia , Vírus Lassa/genética , Vírus Lassa/imunologia , Masculino , Dados de Sequência Molecular , Nucleoproteínas/genética , Filogenia , RNA Viral/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Alinhamento de Sequência , Sibéria/epidemiologiaRESUMO
Starting from the end of 1997, a rise in the epizooty of rabies with peaks in 1998 and 2002 was noted. 550 cases of rabies were registered in wild and domestic animals, as well as one lethal case of rabies in man in 2001. Wild carnivorous animals are traditionally regarded as the reservoir and source of rabies virus (38%). In the Novosibirsk region this disease was registered in badgers, wild herbivorous animals, myomorph rodents, hares, marmots. Urban cases of rabies constituted 23%, cases of rabies among agricultural animals constituted 14% of all rabies cases in the Novosibirsk region.