Lack of age-related mosaic loss of W chromosome in long-lived birds.

Females and males often exhibit different survival in nature, and it has been hypothesized that sex chromosomes may play a role in driving differential survival rates. For instance, the Y chromosome in mammals and the W chromosome in birds are often degenerated, with reduced numbers of genes, and loss of the Y chromosome in old men is associated with shorter life expectancy. However, mosaic loss of sex chromosomes has not been investigated in any non-human species. Here, we tested whether mosaic loss of the W chromosome (LOW) occurs with ageing in wild birds as a natural consequence of cellular senescence. Using loci-specific PCR and a target sequencing approach we estimated LOW in both young and adult individuals of two long-lived bird species and showed that the copy number of W chromosomes remains constant across age groups. Our results suggest that LOW is not a consequence of cellular ageing in birds. We concluded that the inheritance of the W chromosome in birds, unlike the Y chromosome in mammals, is more stable.

Individual variability in diving behavior of the Black-vented Shearwater in an ever-changing habitat.

Oceanic mesoscale systems are characterized by inherent variability. Climatic change adds entropy to this system, making it a highly variable environment in which marine species live. Being at the higher levels of the food chain, predators maximize their performance through plastic foraging strategies. Individual variability within a population and the possible repeatability across time and space may provide stability in a population facing environmental changes. Therefore, variability and repeatability of behaviors, particularly diving behavior, could play an important role in understanding the adaptation pathway of a species. This study focuses on characterizing the frequency and timing of different dives (termed simple and complex) and how these are influenced by individual and environmental characteristics (sea surface temperature, chlorophyll a concentration, bathymetry, salinity, and Ekman transport). This study is based on GPS and accelerometer-recorded information from a breeding group of 59 Black-vented Shearwater and examine consistency in diving behavior at both individual and sex levels across four different breeding seasons. The species was found to be the best performing free diver in the Puffinus genus with a maximum dive duration of 88 s. Among the environmental variables assessed, a relationship was found with active upwelling conditions enhancing low energetic cost diving, on the contrary, reduced upwelling and warmer superficial waters induce more energetically demanding diving affecting diving performance and ultimately body conditions. The body conditions of Black-vented Shearwaters in 2016 were worse than in subsequent years, in 2016, deepest and longest complex dives were recorded, while simple dives were longer in 2017-2019. Nevertheless, the species' plasticity allows at least part of the population to breed and feed during warmer events. While carry-over effects have already been reported, the effect of more frequent warm events is still unknown.

Global assessment of marine plastic exposure risk for oceanic birds.

Plastic pollution is distributed patchily around the world's oceans. Likewise, marine organisms that are vulnerable to plastic ingestion or entanglement have uneven distributions. Understanding where wildlife encounters plastic is crucial for targeting research and mitigation. Oceanic seabirds, particularly petrels, frequently ingest plastic, are highly threatened, and cover vast distances during foraging and migration. However, the spatial overlap between petrels and plastics is poorly understood. Here we combine marine plastic density estimates with individual movement data for 7137 birds of 77 petrel species to estimate relative exposure risk. We identify high exposure risk areas in the Mediterranean and Black seas, and the northeast Pacific, northwest Pacific, South Atlantic and southwest Indian oceans. Plastic exposure risk varies greatly among species and populations, and between breeding and non-breeding seasons. Exposure risk is disproportionately high for Threatened species. Outside the Mediterranean and Black seas, exposure risk is highest in the high seas and Exclusive Economic Zones (EEZs) of the USA, Japan, and the UK. Birds generally had higher plastic exposure risk outside the EEZ of the country where they breed. We identify conservation and research priorities, and highlight that international collaboration is key to addressing the impacts of marine plastic on wide-ranging species.

Mercury exposure in relation to foraging ecology and its impact on the oxidative status of an endangered seabird.

Mercury is a natural element extensively found in the Earth's crust, released to the atmosphere and waters by natural processes. Since the industrial revolution, atmospheric deposition of Hg showed a three-to-five-fold enrichment due to human activities. Marine top predators such as seabirds are recognized valuable bioindicators of ocean health and sensitive victims of Hg toxic effects. Hg negatively affects almost any aspect of avian physiology; thus, birds prove valuable to study the effect of Hg exposure in vertebrates. The Black-vented Shearwater is endemic to the North-Eastern Pacific Ocean, where it forages along the Baja California Peninsula during the breeding period. The area has no industrial settlement and is in the southern portion of the California Current System (CCS). After observing possible contamination effects in eggshells, we decided to quantify the exposure of breeding birds to Hg and test for possible effects on oxidative status of the species. The concentration of Hg in erythrocytes averaged 1.84 µg/g dw and varied from 1.41 to 2.40 µg/g dw. Males and females had similar Hg concentrations. The individual trophic level (reflected by δ15N) did not explain Hg exposure. In contrast, individuals foraging inshore had higher Hg concentrations than those foraging more offshore (reflected by δ13C). Shearwaters having higher concentrations of Hg had lower activity of the antioxidant enzyme glutathione peroxidase and showed lower non-enzymatic antioxidant capacity. Levels of plasma oxidative damage, superoxide dismutase and catalase were not associated with Hg. Our results indicate that (i) the foraging habitat is the factor explaining Hg exposure and (ii) there is some evidence for potential harmful effects of Hg exposure to this seabird species of conservation concern. CAPSULE: The foraging habitat is the factor explaining Hg exposure in seabirds and we observed potential harmful effects of Hg exposure in a seabird species of conservation concern.

A new use of technology to solve an old problem: Estimating the population size of a burrow nesting seabird.

Estimating the population of burrow-nesting seabirds is a challenging task, as human presence in the colony creates disturbances and can damage burrows and occupants. Here, we present a novel method using aerial photographs taken with Unmanned Aerial Vehicles (UAVs) to estimate the population size of a burrow-nesting seabird, the Black-vented Shearwater (Puffinus opisthomelas), on Natividad Island, Mexico. Our results provide a census of burrows in the colony, with very low detection error (5.6%). This is greater accuracy compared to other methods based on extrapolating results from sample plots to total colony area. We then combined this burrow census with ground truth data on occupancy to estimate population size. We obtained a population estimate of 37,858 and 46,322 breeding pairs for 2016 and 2017 respectively. The proposed method provides a cost effective and repeatable approach for monitoring numbers of burrows occupied in a colony, thereby enabling easier and faster estimates of population trends. We suggest this method can be valid for other burrow-nesting species in habitats without dense vegetation cover.

Effects of human disturbance on cave-nesting seabirds: the case of the storm petrel.

Human disturbance is an important stress factor with potentially strong impact on breeding activity in animals. The consequences can be extinction of the breeding population, because disturbed animals might desert their breeding area and find no suitable substitute area. In this study, we investigated the effects of anthropogenic disturbance on a breeding population of Mediterranean storm petrels. Seabirds are increasingly used as bio-indicators for sea environmental parameters, because they are very sensitive to changing conditions. Burrowing or cave-nesting species may be particularly susceptible to human disturbance because their direct contact with humans is usually minimal or absent. First, we compared two different populations (exposed or not exposed to human disturbance) for their individual stress response to a standardized stressor (handling and keeping in a cloth bag). Second, we compared the two sub-colonies for their population-level stress response. Third, we tested experimentally whether sub-colonies of storm petrels exposed to tourism have physiological adaptations to anthropogenic disturbances. Our results indicate that storm petrels may be habituated to moderate disturbance associated with boat traffic close to the colony.