Streptomyces profundus sp. nov., a novel marine actinobacterium isolated from deep-sea sediment of Madeira Archipelago, Portugal.

A novel strain, MA3_2.13T, was isolated from deep-sea sediment of Madeira Archipelago, Portugal, and characterized using a polyphasic approach. This strain produced dark brown soluble pigments, bronwish black substrate mycelia and an aerial mycelium with yellowish white spores, when grown on GYM 50SW agar. The main respiratory quinones were MK-10(H4), MK-10(H6) and MK-10(H8). Diphosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids and two glycophospholipids were identified as the main phospholipids. The major cellular fatty acids were iso-C16 : 1, iso-C16 : 0, anteiso-C17 : 1 and anteiso-C17 : 0. Phylogenetic analyses based on 16S rRNA gene showed that strain MA3_2.13T is a member of the genus Streptomyces and was most closely related to Streptomyces triticirhizae NEAU-YY642T (NR_180032.1; 16S rRNA gene similarity 97.9 %), Streptomyces sedi YIM 65188T (NR_044582.1; 16S rRNA gene similarity 97.4 %), Streptomyces mimosae 3MP-10T (NR_170412.1; 16S rRNA gene similarity 97.3 %) and Streptomyces zhaozhouensis NEAU-LZS-5T (NR_133874.1; 16S rRNA gene similarity 97.0 %). Genome pairwise comparisons with closest related type strains retrieved values below the threshold for species delineation suggesting that strain MA3_2.13T represents a new branch within the genus Streptomyces. Based on these results, strain MA3_2.13T (=DSM 115980T=LMG 33094T) is proposed as the type strain of a novel species of the genus Streptomyces, for which the name Streptomyces profundus sp. nov. is proposed.

Did I text you? The influence of the mode of transmission on destination memory.

In a society where people often communicate through digital technology, it is crucial to investigate whether the transmission mode influences destination memory performance (our capacity to remember to whom we transmitted certain information). In Experiment 1, we asked young adults (N = 31) to share of a set of familiar proverbs only by typing and the rest by both typing and saying them aloud. Better destination memory was observed when the information was transmitted by the two means (aloud and typing). Did this better performance occur because participants shared the information aloud or because the information was transmitted by two means? In Experiment 2, young adults (N = 34) shared familiar proverbs aloud, by typing, or simultaneously aloud and by typing. Results showed that transmission aloud led to a better destination memory than typing, and no further improvement occurred when the transmission was both aloud and by typing. Additionally, no differences were observed regarding item memory, supporting the idea that item and destination memories are two different types of memory.

Integrating Cosmic Microwave Background Readings with Celestial Navigation to Enhance Deep Space Navigation.

This research unveils a cutting-edge navigation system for deep space missions that utilizes cosmic microwave background (CMB) sensor readings to enhance spacecraft positioning and velocity estimation accuracy significantly. By exploiting the Doppler-shifted CMB spectrum and integrating it with optical measurements for celestial navigation, this approach employs advanced data processing through the Unscented Kalman Filter (UKF), enabling precise navigation amid the complexities of space travel. The simulation results confirm the system's exceptional precision and resilience in deep space missions, marking a significant advancement in astronautics and paving the way for future space exploration endeavors.

The role of cue salience in prospective memory commission errors in nonperformed nonfocal tasks.

Prospective memory (PM) refers to the ability to remember to execute an intention in the future without having a permanent reminder. These intentions can be performed when they are not relevant or become no-longer needed, the so-called "commission errors". The present study aims to understand the effect of cue salience on PM commission errors with unperformed intentions and on the ongoing task performance-associated costs. Through a between-subjects design, eighty-one participants were assigned to 3 conditions: the no-PM condition, which served as control, and the salient and nonsalient conditions, which were asked to perform a lexical decision task and an incomplete nonfocal prospective memory task (i.e. no PM cues were presented). Subsequently, participants were instructed to no longer execute the prospective intention. In the second phase, a lexical decision task occurred again, including irrelevant PM cues, which should not be answered as such. In the salient condition, cues were salient (i.e. presented in red or blue background). In contrast, in the nonsalient condition, PM cues appeared on a black background, as any other stimuli. In the no-PM control condition, participants only performed an LDT. A commission error occurred when the (irrelevant) intention was performed in this second phase. Results showed that more participants performed a commission error in the presence of salient cues, even when PM intentions became irrelevant. Additionally, when cues were not salient, participants took longer to answer the LDT, as reasoned by the spontaneous retrieval theory. These findings are discussed according to the dual-mechanism account.

Collaborative inhibition effect: the role of memory task and retrieval method.

It is well established that the recall of collaborative groups is lower than the pooled recall of an equal number of lone individuals-the collaborative inhibition effect (Weldon and Bellinger, J Exp Psychol Learn Memory Cogn 23(5):1160-1175, 1997). This is arguably the case because group members have conflicting retrieval strategies that disrupt each other's recall-the retrieval strategies disruption hypothesis (Basden et al., J Exp Psychol Learn Memory Cogn 23(5):1176-1191, 1997). In two experiments, we further examined this hypothesis by testing whether the memory task (free recall vs. serial recall) and the recall method (turn-taking vs. unconstraint) moderate collaborative inhibition. Experiment 1 compared the performance of collaborative and nominal groups in a free recall and a serial recall task. Results revealed collaborative inhibition in free recall, but this effect was reduced in serial recall. In Experiment 2, collaborative and nominal performance was compared in the same tasks with collaborative but also nominal groups, using the turn-taking method. The collaborative inhibition effect was still observed in free recall, although to a lesser extent when participants in nominal groups used the turn-taking method. In the serial recall task, the collaborative inhibition effect was eliminated. Taken together, these results further support retrieval strategies disruption as an explanation for the collaborative inhibition effect.

The impact of the use of masks on trait judgments and face recognition.

Although effective in reducing virus transmission, face masks might compromise face recognition and trait judgments. With this study, we aimed to observe the influence of masks on face recognition and trait judgments-more specifically, in trustworthiness, dominance, and distinctiveness judgments. Also, we wanted to observe the possible influence of trait judgments on facial recognition for masked and unmasked faces, which has never been done before. For that, we conducted an online study where 140 participants observed and made trait judgments of masked and unmasked faces in a within-subjects design. After a distractive task, participants performed a recognition memory test. As expected, we observed a better recognition of faces shown without a mask during the study phase, which allowed the holistic processing of the faces. The worst performance was found for faces encoded with a mask but tested without it, occurring simultaneity disruption in holistic face processing and the violation of the encoding specificity principle. Regarding the trait judgments, unmasked faces were considered more distinctive, and masked faces were considered more trustworthy. More interestingly, we can conclude that facial distinctiveness predicts face recognition, regardless of mask use. In contrast, dominance judgments only predicted face recognition when faces were presented without a mask. When faces were exposed with masks, trustworthiness overrides dominance, becoming more critical to recognizing faces. We can interpret these results from an evolutionary perspective.

Remembering to whom we transmit information during pandemics: the effect of face masks on destination memory.

Considering the global pandemic we currently experience, face masks have become standard in our daily routine. Even though surgical masks are established as a safety measure against the dissemination of COVID-19, previous research showed that their wearing compromises face recognition. Consequently, the capacity to remember to whom we transmit information-destination memory-could also be compromised. In our study, through a between-participants design (experiment 1) and a within-participants design (experiment 2), undergraduate students have to transmit Portuguese proverbs to masked and unmasked celebrity faces. Following our hypothesis, participants who shared information with masked faces had worse destination memory performance than those who shared information with unmasked faces. Also, we observed lower recognition for masked faces compared to unmasked faces. These results were expected since using a surgical mask affects facial recognition, thus making it harder to recognize a person to whom information was previously transmitted. More importantly, these results also support the idea that variables associated with the recipient's face are important for destination memory performance.

"Choose it, and remember it": The impact of choice on destination memory.

Destination memory can be defined as the capacity to remember to whom we transmit information. It is measured through the accuracy of retrieving the association between the information we transmit and the person to whom we transmit it. A destination memory procedure aims to emulate human interaction by sharing facts with celebrities (i.e., familiar faces) since we often communicate with people we know. However, the role of the choice about who we intend to transmit the information to has not been evaluated before. This paper investigated whether deciding with whom to share a piece of information benefits destination memory. We designed two experiments with different levels of cognitive load, increasing it from Experiment 1 to Experiment 2. The experiments included two conditions: the choice condition, in which participants chose from two options to whom they desired to share a fact, and the no-choice condition, in which participants simply shared facts with celebrities without the possibility of a choice. Experiment 1 suggested that a choice component did not affect destination memory. However, when in Experiment 2 we raised the cognitive load by increasing the number of stimuli, we found that selecting the recipient during the more challenging task provided an advantage in destination memory. This result is congruent with the explanation that the shift of the participants' attentional resources to the recipient, caused by the choice component, leads to a destination memory improvement. In sum, it seems that a choice component can improve destination memory only under demanding attentional conditions.

Stimuli in 3 Acts: A normative study on action-statements, action videos and object photos.

The study of action observation and imagery, separately and combined, is expanding in diverse research areas (e.g., sports psychology, neurosciences), making clear the need for action-related stimuli (i.e., action statements, videos, and pictures). Although several databases of object and action pictures are available, norms on action videos are scarce. In this study, we validated a set of 60 object-related everyday actions in three different formats: action-statements, and corresponding dynamic (action videos) and static (object photos) stimuli. In Study 1, ratings of imageability, image agreement, action familiarity, action frequency, and action valence were collected from 161 participants. In Study 2, a different sample of 115 participants rated object familiarity, object valence, and object-action prototypicality. Most actions were rated as easy to imagine, familiar, and neutral or positive in valence. However, there was variation in the frequency with which participants perform these actions on a daily basis. High agreement between participants' mental image and action videos was also found, showing that the videos depict a conventional way of performing the actions. Objects were considered familiar and positive in valence. High ratings on object-action prototypicality indicate that the actions correspond to prototypical actions for most objects. 3ActStimuli is a comprehensive set of stimuli that can be useful in several research areas, allowing the combined study of action observation and imagery.

Concentrations of iron and chromium in free-ranging common marmosets (Callithrix jacchus) from Pernambuco, Brazil.

Common marmosets (Callithrix jacchus) are widely distributed in Northeast Brazil and often inhabit urban and peri-urban forest areas close to human settlements. Given its wide territorial distribution, its proximity to human populations, and its exposure to environmental degradations originating from urbanization, common marmosets have a high potential for environmental biomonitoring. The concentrations of iron (Fe) and chromium (Cr) were quantified in the liver, hair, and bone of 22 free-ranging common marmosets' bodies from nine cities from Pernambuco State, Brazil, using inductively coupled plasma optical emission spectrometry (ICP OES). The liver showed the highest concentrations of Fe and Cr (3773.2 ± 3715.8 mg/kg and 19.4 ± 41.6 mg/kg, respectively); the lowest concentration of Fe was detected in the bone (111.6 ± 97.6 mg/kg) and of Cr in the hair (3.3 ± 1.5 mg/kg). There was a moderate positive correlation between Fe and Cr in the liver (r = 0.64) and a high negative correlation for Cr between bone and hair (r = -0.65). This study demonstrated the bioaccumulation of Fe and Cr in hair, liver, and bone in common marmosets. The highest average concentration of Fe and Cr occurred in animals from Recife, Jaboatão dos Guararapes, and Paulista, respectively, the 1st, the 2nd, and the 5th most populated cities in the state of Pernambuco. The presence of high concentrations of metals in animals from Recife and nearby cities can indicate alarming levels of environmental pollution in these locations.

Headache, anosmia, ageusia and other neurological symptoms in COVID-19: a cross-sectional study.

BACKGROUND: Neurological symptoms are frequent among patients with COVID-19. Little is known regarding the repercussions of neurological symptoms for patients and how these symptoms are related to one another. OBJECTIVES: To determine whether there is an association between the neurological symptoms in patients with COVID-19, and to characterize the headache. METHOD: This was a cross-sectional study. All hospital inpatients and health workers at the Hospital Universitario Oswaldo Cruz with a PCR-confirmed COVID-19 infection between March and June 2020 were considered for the study and were interviewed by telephone at least 2-months after the acute phase of the disease. These patients were identified by the hospital epidemiological surveillance department. A semi-structured questionnaire was used containing sociodemographic and clinical data and the ID-Migraine. RESULTS: A total of 288 patients was interviewed; 53.1% were male; with a median age of 49.9 (41.5-60.5) years; 91.7% presented some neurological symptom; 22.2% reported some neurological symptom as the symptom that troubled them most during COVID-19. Neurological symptoms were: ageusia (69.8%), headache (69.1%), anosmia (67%), myalgia (44.4%), drowsiness (37.2%), agitation (20.8%); mental confusion (14.9%), syncope (4.9%) and epileptic seizures (2.8%). Females, those who presented with fever, sore throat, anosmia/ageusia and myalgia also presented significantly more with headache (logistic regression). The most frequent headache phenotype was a non-migraine phenotype, was of severe intensity and differed from previous headaches. This persisted for more than 30 days in 18% and for more than 90 days in 10% of patients. Thirteen percent of those with anosmia and 11% with ageusia continued with these complaints after more than 90 days of the acute phase of the disease. Aged over 50 years, agitation and epileptic seizures were significantly associated with mental confusion (logistic regression). CONCLUSION: Headache is frequent in COVID-19, is associated with other symptoms such as fever, sore throat, anosmia, ageusia, and myalgia, and may persist beyond the acute phase of the disease.

Emergence and transmission of misinformation in the context of social interactions.

The emergence and transmission of false memories is well documented in individual memory tasks. However, the examination of these processes in the context of social interaction still presents mixed findings. The present study further examines the potential of collaboration in minimizing the acceptance and retrieval of misinformation. In Experiment 1 participants watched a video immediately followed by a recall task (collaborative vs. nominal). Then a questionnaire (collaborative vs. individual) containing true information and misinformation about the video was presented. After the questionnaire, participants were given a new recall task (collaborative vs. nominal). We expected that collaboration at encoding and at retrieval would reduce the acceptance and recall of misinformation. Results revealed, as expected, that collaborative groups performed better in answering the questionnaire, accepting more correct information and rejecting more misinformation. Subsequently, they also recalled less misinformation. However, their recall of correct information was also lower. To rule out the potential role of collaborative inhibition in explaining the results observed in the final recall, in Experiment 2 the collaborative manipulation occurred only during the questionnaire and both recall tasks were individual. Again, participants answering the questionnaire collaboratively performed better than those answering individually. Critically, in a subsequent individual recall task, they produced less false memories and more correct information than those answering the questionnaire individually. These results suggest that collaboration during information encoding reduces the acceptance of misinformation and its subsequent recall.

Expression cassette and plasmid construction for Yeast Surface Display in Saccharomyces cerevisiae.

OBJECTIVES: Develop a Cell Surface Display system in Saccharomyces cerevisiae, based on the construction of an expression cassette for pYES2 plasmid. RESULTS: The construction of an expression cassette containing the α-factor signal peptide and the C-terminal portion of the α-agglutinin protein was made and its sequence inserted into a plasmid named pYES2/gDαAgglutinin. The construction allows surface display of bovine herpesvirus type 5 (BoHV-5) glycoprotein D (gD) on S. cerevisiae BY4741 strain. Recombinant protein expression was confirmed by dot blot, and indirect immunofluorescence using monoclonal anti-histidine antibodies and polyclonal antibodies from mice experimentally vaccinated with a recombinant gD. CONCLUSIONS: These results demonstrate that the approach and plasmid used represent not only an effective system for immobilizing proteins on the yeast cell surface, as well as a platform for immunobiologicals development.

Complete Genome Sequence of Two Deep-Sea Streptomyces Isolates from Madeira Archipelago and Evaluation of Their Biosynthetic Potential.

The deep-sea constitutes a true unexplored frontier and a potential source of innovative drug scaffolds. Here, we present the genome sequence of two novel marine actinobacterial strains, MA3_2.13 and S07_1.15, isolated from deep-sea samples (sediments and sponge) and collected at Madeira archipelago (NE Atlantic Ocean; Portugal). The de novo assembly of both genomes was achieved using a hybrid strategy that combines short-reads (Illumina) and long-reads (PacBio) sequencing data. Phylogenetic analyses showed that strain MA3_2.13 is a new species of the Streptomyces genus, whereas strain S07_1.15 is closely related to the type strain of Streptomyces xinghaiensis. In silico analysis revealed that the total length of predicted biosynthetic gene clusters (BGCs) accounted for a high percentage of the MA3_2.13 genome, with several potential new metabolites identified. Strain S07_1.15 had, with a few exceptions, a predicted metabolic profile similar to S. xinghaiensis. In this work, we implemented a straightforward approach for generating high-quality genomes of new bacterial isolates and analyse in silico their potential to produce novel NPs. The inclusion of these in silico dereplication steps allows to minimize the rediscovery rates of traditional natural products screening methodologies and expedite the drug discovery process.

A Spatiotemporal Deep Learning Approach for Automatic Pathological Gait Classification.

Human motion analysis provides useful information for the diagnosis and recovery assessment of people suffering from pathologies, such as those affecting the way of walking, i.e., gait. With recent developments in deep learning, state-of-the-art performance can now be achieved using a single 2D-RGB-camera-based gait analysis system, offering an objective assessment of gait-related pathologies. Such systems provide a valuable complement/alternative to the current standard practice of subjective assessment. Most 2D-RGB-camera-based gait analysis approaches rely on compact gait representations, such as the gait energy image, which summarize the characteristics of a walking sequence into one single image. However, such compact representations do not fully capture the temporal information and dependencies between successive gait movements. This limitation is addressed by proposing a spatiotemporal deep learning approach that uses a selection of key frames to represent a gait cycle. Convolutional and recurrent deep neural networks were combined, processing each gait cycle as a collection of silhouette key frames, allowing the system to learn temporal patterns among the spatial features extracted at individual time instants. Trained with gait sequences from the GAIT-IT dataset, the proposed system is able to improve gait pathology classification accuracy, outperforming state-of-the-art solutions and achieving improved generalization on cross-dataset tests.

From retrospective to prospective memory research: a framework for investigating the deactivation of intentions.

The definition of episodic memory has evolved into a multifaceted concept that gathered great attention in several research areas in psychology and neuroscience. Prospective memory (PM), or the ability to remember to perform delayed intentions at a later moment in the future, represents one side of this capacity for which that has been a growing interest. In this review, we examined a counterintuitive finding: PM intentions may persist and affect our behaviour despite successful goal attainment and task completion, which in daily life may be as serious as taking medication twice. This review aims to elucidate the existing knowledge and identify some unresolved questions concerning this specific memory failure. Therefore, this review provides an overview of the uprising research dedicated to both PM omission and commission errors, including an analysis of its definitions, of the current theoretical approaches of PM retrieval, and the main procedures used in this field to offer an integrative perspective on this topic. Finally, the last section is devoted to discussing future directions to test the predictions of our suggested theoretical explanations for PM deactivation. This might be an avenue for research that is likely to extend our understanding of episodic memory's usefulness in everyday life.

The effect of distinctive facial features on destination memory.

Destination memory involves remembering to whom we told information. Low accuracy of this memory is linked to higher self-focus and lower attentional resources allocated to the recipient of the information. The present paper aimed to investigate whether the existence of distinctive features (e.g., tattoos) of destination face would improve destination memory, in a within- (Experiment 1 and 2) and between-participants (Experiment 3) design. In a destination memory task, participants had to tell proverbs to faces that presented a distinctive feature and to other faces that did not. Results showed that a destination memory advantage only occurs when faces with different distinctive features are compared to faces without distinctive features (Experiment 1). These results are in accordance with the existing theoretical framework on destination memory and distinctiveness; highlighting the importance of personal attributes, namely, the distinctiveness of the destination face and the relativity of distinctiveness in destination memory.

Recognition and naming test of the Portuguese population for national and international celebrities.

Research on familiar faces has been conducted in different countries and resort to celebrities faces, stimuli that are highly constrained by geographic context and cultural peculiarities, since many celebrities are only famous in particular countries. Despite their relevance to psychological research, there are no normative studies of celebrities' facial recognition in Portugal. We developed a database with 160 black and white pictures of famous persons' faces in this work. The data collection took place in two different studies. In study 1, participants were asked to recognize and name celebrity faces; while in study 2, celebrity names were rated for AoA, familiarity, and distinctiveness. Data were gathered from two different samples of Portuguese young adults aged between 18 and 25 years old, and both procedures were performed online through a questionnaire created in Qualtrics software. This database provides ratings of AoA, familiarity, facial distinctiveness, recognition rate, and naming rate for each celebrity, which will allow further selection of celebrities, based on these five attributes, for studies using Portuguese samples. Also, possible relationships between these five variables were analyzed and presented, highlighting facial distinctiveness as a predictor for both naming and recognition rate of celebrity faces.

When we must forget: the effect of cognitive load on prospective memory commission errors.

Recent studies consistently show that prospective memory (PM) intentions are not always deactivated when no-longer needed and might be erroneously performed upon encountering the once relevant cue - termed PM commission errors. However, empirical evidence on the potential mechanisms that might lead to this kind of memory failure remains mostly unexplored. This study aimed to investigate the influence of the ongoing task demands on PM deactivation of non-performed intentions. Younger adults, except for those in the no-PM condition, were asked to perform a PM task and were then told that the intention was finished. Later, they perform a lexical decision task with some trials containing (irrelevant) PM cues while simultaneously carrying out a counting recall task with two levels of difficulty. The results showed a higher risk of PM commission errors under moderate cognitive load (74%) as compared to the no-load condition (40%). Results also show that commission error risk did not increase in the high-load (54%) compared with the moderate-load condition. Furthermore, comparisons of the ongoign task performance between the no-PM condition and the other conditions with a PM task requirement support that commission errors might arise from a spontaneous PM retrieval. The implications of these findings are discussed within the dual-mechanisms account.

Cognitive load eliminates the effect of perceptual information on judgments of learning with sentences.

Items presented in large font are rated with higher judgments of learning (JOLs) than those presented in small font. According to current explanations of this phenomenon in terms of processing fluency or implicit beliefs, this effect should be present no matter the type of material under study. However, we hypothesized that the linguistic cues present in sentences may prevent using font size as a cue for JOLs. Experiment 1, with short sentences, showed the standard font-size effect on JOLs, and Experiment 2, with pairs of longer sentences, showed a reduced effect. These results suggest that linguistic factors do not prevent font size from being used for JOLs. However, Experiment 3, with both short and long sentences, showed an effect of font size only for the former and not the latter condition, suggesting that the greater amount of to-be-remembered information eliminated the font-size effect. In Experiment 4, we tested a mechanism to explain this result and manipulated cognitive load using the dot-memory task. The short sentences from Experiments 1 and 3 were used, and the results replicated the font-size effect only in the low-cognitive load condition. Our results are consistent with the idea that perceptual information is used to make JOLs only with materials such as words, word pairs, or short sentences, and that the increased cognitive load required to process longer sentences prevents using font size as a cue for JOLs.