Encapsulated peritoneal sclerosis.

Encapsulating peritoneal sclerosis (EPS) also known as abdominal cacoon is a rare cause of acute or subacute small bowel obstruction. It is characterized by total or partial encasement of the small bowel within a thick fibrocollagenous membrane which may be formed in response to prolonged, repetitive, and severe insult to the peritoneal mesothelium. This is frequently seen in the setting of peritoneal dialysis. However other causes may include chronic inflammation. We present a case of EPS in a male with infrequent abdominal pain, nausea and fever.

Fabrication of a salivary amylase electrochemical sensor based on surface confined MWCNTs/ß-cyclodextrin/starch architect for dental caries in clinical samples.

Salivary α-amylase (α-ALS) has drawn attention as a possible bioindicator for dental caries. Herein, combining the synergistic properties of multi-walled carbon nanotubes (MWCNTs), ß-cyclodextrin (ß-CD) and starch, an electrochemical sensor is constructed employing ferrocene (FCN) as an electrochemical indicator to oversee the progression of the enzymatic catalysis of α-ALS. The method involves a two-step chemical reaction sequence on a screen-printed carbon electrode (SPCE). X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, Field emission scanning electron microscope (FE-SEM), and Dynamic light scattering (DLS) were used to characterize the synthesized material, while Static water Contact angle measurements, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) were performed to monitor each step of sensor fabrication. The electrochemical sensor permitted to detect α-ALS within the linear range of 0.5-280 U mL-1, revealing detection (LOD), and quantification (LOQ) values of 0.041 U mL-1, and 0.159 U mL-1, respectively. Remarkably, the sensor demonstrated exceptional specificity and selectivity, effectively discriminating against other interfering substances in saliva. Validation of the method involved analyzing α-ALS levels in artificial saliva with an accuracy range of 97 % to 103 %, as well as in real clinical saliva samples across various age groups.

Host-Guest Mechanism via Induced Fit Fullerene Complexation in Porphin Receptor to Probe Salivary Alpha-Amylase in Dental Caries for Clinical Applications.

Salivary α-amylase is the most abundant protein of human saliva that potentially binds to streptococcus and other bacteria via specific surface-exposed α-amylase-binding proteins and plays a significant role in caries development. The detection of α-amylase in saliva can be used as a bioindicator of caries development. Herein, a facile strategy has been applied, tailoring the photochemical properties of 5,10,15,20-tetrakis(4-hydroxyphenyl)-21H,23H-porphine (TPPOH) and the fullerene C60 complex. The fluorescence emission of TPPOH is quenched by starch-coated fullerene C60 via charge-transfer effects, as determined by UV absorption and fluorescence spectroscopic studies. The starch-coated C60 has been thoroughly characterized via Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), optical microscopy, thermal gravimetric analysis (TGA), static water contact angle measurements, and zeta potential measurements. The analytical response of the assay showed a linear fluorescent response in α-amylase concentrations ranging from 0.001-0.1 Units/mL, with an LOD of 0.001 Units/mL. The applicability of the method was tested using artificial saliva with quantitative recoveries in the range 95-100%. The practicability of the procedure was verified by inspecting saliva samples of real clinical samples covering all age groups. We believe that the proposed method can serve as an alternative analytical method for caries detection and risk assessment that would also minimize the cost of professional preventive measures and treatments.

Assessment of Periodontal Health Status and Treatment Needs Among Pregnant Women.

Background Pregnancy-related periodontal health is vital for maternal and fetal well-being, with implications on birth outcomes. However, comprehensive data on periodontal health among pregnant women in Pakistan are lacking. This research aimed to assess the periodontal health status and treatment needs among pregnant women in Pakistan. Methodology This study conducted at Multan Medical and Dental College in Multan, Pakistan, utilized a cross-sectional design over one year from January 2023 to December 2023. It enrolled 230 pregnant women from the prenatal care clinic, employing strict inclusion and exclusion criteria to ensure data integrity. Data collection involved a systematic questionnaire and clinical examination by qualified dental practitioners, covering demographic details, obstetric history, dental hygiene habits, and periodontal health parameters. Statistical analysis included descriptive statistics, chi-square tests, and logistic regression to evaluate periodontal health status determinants. Results This study, comprising 230 participants, delineates key demographic and periodontal health indicators. Notably, the age group between 26 and 30 years made up the greatest percentage (n = 87, 37.83%), followed by the age group between 18 and 25 years (n = 58, 25.22%). The chi-square test showed a significant association between age and periodontal health (χ² = 8.23, df = 3, p = 0.041). University-educated participants showed decreased periodontal risks (odds ratio = 0.51, p = 0.037), with education level also emerging as a significant factor (χ² = 12.76, df = 2, p = 0.002). Regarding dental hygiene, 44.35% of people brushed twice a day, and 27.83% flossed every day. Periodontal data revealed that 53.04% of individuals had gingivitis and that the mean probing depth was 3.22 mm. Scaling and root planing were the most requested therapy (50.00%). Conclusions This study provides valuable insights into the periodontal health status and treatment needs of pregnant women in Pakistan. Investigative analyses including chi-square tests and logistic regression identified significant associations between demographic factors, oral hygiene practices, and periodontal health outcomes among pregnant women in Pakistan, emphasizing the need for tailored interventions to enhance maternal and child health.