Shallow-depth sequencing of cell-free DNA for Hodgkin and diffuse large B-cell lymphoma (differential) diagnosis: a standardized approach with underappreciated potential.

Shallow-depth sequencing of cell-free DNA, a cheap and standardized approach to obtain molecular information on tumors non-invasively, is insufficiently explored for lymphoma diagnosis and disease follow-up. This study collected 318 samples, including longitudinal liquid and paired solid biopsies, from a prospectively recruited cohort of 38 Hodgkin lymphoma (HL) and 85 aggressive B-cell non- HL patients, represented by 81 diffuse large B-cell lymphoma (DLBCL) cases. Following sequencing, copy number alterations and viral read fractions were derived and analyzed. At diagnosis, liquid biopsies showed detectable copy number alterations in 84.2% of HL (88.6% for classical HL) and 74.1% of DLBCL patients. Copy number profiles between liquid-solid pairs were highly concordant within DLBCL (r=0.815±0.043); and, compared to tissue, HL liquid biopsies had abnormalities with higher amplitudes (P=.010), implying that tumor DNA is more abundant in plasma. Additionally, 39.5% of HL and 13.6% of DLBCL cases had a significantly elevated number of plasmatic Epstein-Barr virus DNA fragments, achieving a sensitivity of 100% compared to current standard. Longitudinal analysis determined that, when detectable, copy number patterns were similar across (re)staging moments in refractory/relapsed patients. Moreover, the overall profile anomaly highly correlated with the total metabolic tumor volume (P.

Deficient inhibition of emotional information in depression.

BACKGROUND: There are numerous indications that impaired inhibition of negative affective material could be an important cognitive component of depression. To study whether impaired inhibition of negative affect is a cognitive vulnerability factor explaining (recurrent) depression, inhibition of positive and negative affective stimuli was examined in hospitalized depressed patients, formerly depressed individuals and never-depressed controls. METHODS: To investigate inhibitory dysfunctions in the processing of emotional material, we used an affective modification of the negative priming task with pictures of sad and happy facial expressions. RESULTS: Compared to never-depressed controls, depressed patients showed a specific failure to inhibit negative information, whereas inhibition function for positive material was unaffected. Surprisingly, formerly depressed individuals demonstrated impaired inhibition of negative and positive information. LIMITATIONS: Because of the significant correlations between depression and anxiety self-report scores, the observed reduced inhibitory effect toward negative material in the depression group cannot strictly be attributed as depression-specific. CONCLUSIONS: In accordance with our hypothesis, strongly impaired inhibition of negative affect was found in depressed patients. Based on the present findings, we argue that impaired inhibition of negative affect could be an important construct in cognitive theories on depression linking cognitive biases to neuropsychological impairments in depression. The data in the formerly depressed individuals are less conclusive and several hypotheses are detailed that could explain how the absence of inhibition of affective information could relate to recurrent depression.

The effects of attentional training on physiological stress recovery after induced social threat.

Social stress has been related to both physiological and psychological responses. The recovery of stress is influenced by the way environmental information is processed, i.e., what information is attended to and how it is interpreted. The present study investigated the effect of attention modification training on physiological stress recovery following induced stress. A group of applicants preparing for a job interview were randomly allocated to an attention training condition versus a control condition. Afterwards, they were asked to give an artificial job interview to a real life jury while being videotaped. Participants in both conditions showed decreased heart rate variability during the job interview, which indicates an overall physiological stress response. However, during a 30-minute follow-up period after the training, indices of heart rate variability showed improved stress recovery only in the attention training condition. Attentional training is proposed as a promising strategy to prevent enduring stress reactions.

Attentional retraining procedures: manipulating early or late components of attentional bias?

According to cognitive models of anxiety disorders, attentional bias for threatening information is a vulnerability factor to the etiology and maintenance of anxiety. A recently developed methodology to reduce attentional bias has been found to reduce emotional reactivity and anxiety. The present study aimed at identifying the effects of this attentional bias reduction on early and later stages of threat processing. Undergraduates were allocated to an attentional bias reduction (n = 23) versus control condition (n = 25). It was found that attentional bias reduction influenced late but not early stages of threat processing. This finding is of theoretical importance in relation to studies on the causal role of attentional bias and emotional reactivity. Moreover, the present findings also bear relevance to the clinical application of attentional retraining procedures.

Attentional bias training in depression: therapeutic effects depend on depression severity.

Depressed individuals show maintained attention to negative information and reduced attention for positive information. Selective biases in information processing are considered to have an important role in the origin, maintenance and recurrence of depressive episodes. In two experiments we investigated the effects of attentional bias manipulation on mood and depressive symptoms. In experiment 1 we investigated the effects of attentional bias manipulation compared to a control procedure in a sample of dysphoric students (N = 48) showing mild to severe levels of depressive symptoms. In experiment 2 we investigated the same attentional training procedure in a sample of depressed in- and outpatients (N = 35). Mild improvements on symptom severity were observed in students showing mild depressive symptoms. However, in students showing moderate to severe depressive symptoms, depressive symptoms increased after the training. No beneficial effects of training on top of therapy and/or medication were found in depressed patients. These results indicate that therapeutic effects of attentional bias modification might be dependent on depression severity.