RESUMO
OBJECTIVE: To prepare and characterize marine sterically stabilized liposomes (Marine-SSL). METHODS: Liposomes were prepared by ethanol injection technique. An orthogonal test was utilized to optimize the formulation and preparation of Marine-SSL The unencapsulated marine and liposomes were separated by sephadex gel G-50, the encapsulation efficiency was detected by HPLC. The morphological examination of Marine-SSL was performed using transmission electron microscopy. The particle size and Zeta potential of the liposomes were measured. The in vitro release rate of marine from liposomes was tested. RESULTS: The liposomes with spherical or ellipsoidal shape and better stability featured the encapsulation efficiency of (85.39 +/- 1.21)%, the mean partical size of (156 +/- 10) nm, and Zeta potential of (- 39.0 +/- 3.06) mv. The release kinetics in vitro obeyed Higuchi equation. The stability of Marine-SSL was better. CONCLUSION: The selected formulation and preparation technic of Marine-SSL are rational and stable and liposomes feature a sustained release in vitro.