Type 1 Treg cells promote the generation of CD8+ tissue-resident memory T cells.

Tissue-resident memory T (TRM) cells, functionally distinct from circulating memory T cells, have a critical role in protective immunity in tissues, are more efficacious when elicited after vaccination and yield more effective antitumor immunity, yet the signals that direct development of TRM cells are incompletely understood. Here we show that type 1 regulatory T (Treg) cells, which express the transcription factor T-bet, promote the generation of CD8+ TRM cells. The absence of T-bet-expressing type 1 Treg cells reduces the presence of TRM cells in multiple tissues and increases pathogen burden upon infectious challenge. Using infection models, we show that type 1 Treg cells are specifically recruited to local inflammatory sites via the chemokine receptor CXCR3. Close proximity with effector CD8+ T cells and Treg cell expression of integrin-ß8 endows the bioavailability of transforming growth factor-ß in the microenvironment, thereby promoting the generation of CD8+ TRM cells.

CD155/PVR determines acute myeloid leukemia targeting by Delta One T cells.

ABSTRACT: Relapsed or refractory acute myeloid leukemia (AML) remains a major therapeutic challenge. We have recently developed a Vδ1+ γδ T cell-based product for adoptive immunotherapy, named Delta One T (DOT) cells, and demonstrated their cytolytic capacity to eliminate AML cell lines and primary blasts in vitro and in vivo. However, the molecular mechanisms responsible for the broad DOT-cell recognition of AML cells remain poorly understood. Here, we dissected the role of natural killer (NK) cell receptor ligands in AML cell recognition by DOT cells. Screening of multiple AML cell lines highlighted a strong upregulation of the DNAM-1 ligands, CD155/pulmonary vascular resistance (PVR), CD112/nectin-2, as well as the NKp30 ligand, B7-H6, in contrast with NKG2D ligands. CRISPR-mediated ablation revealed key nonredundant and synergistic contributions of PVR and B7-H6 but not nectin-2 to DOT-cell targeting of AML cells. We further demonstrate that PVR and B7-H6 are critical for the formation of robust immunological synapses between AML and DOT cells. Importantly, PVR but not B7-H6 expression in primary AML samples predicted their elimination by DOT cells. These data provide new mechanistic insight into tumor targeting by DOT cells and suggest that assessing PVR expression levels may be highly relevant to DOT cell-based clinical trials.

Abnormal right ventricular echocardiographic findings in recovered patients associated with severe acute respiratory syndrome in COVID-19.

BACKGROUND: Right ventricular (RV) echocardiographic changes such as dilation or systolic dysfunction, and pulmonary arterial hypertension were observed in patients with COVID-19. The aim of our study was to determine the frequency of RV echocardiographic changes in patients who have recovered from COVID-19 and to verify the association between severe acute respiratory syndrome (SARS) and echocardiographic findings. METHODS: Patients who had recovered from COVID-19 undergoing outpatient follow-up underwent transthoracic echocardiography, and based on the findings, were divided into two groups: normal and abnormal. It was then verified whether there is an association between SARS and RV echocardiographic abnormalities in recovered patients. RESULTS: The study included 61 patients, with a mean age of 54.2 ± 12.0 years, 57.4% had presented with SARS. The mean period of time between COVID-19 and the echocardiographic examination was 11.9 ± 7.0 months. Patients presented normal left ventricular systolic function. The frequency of RV echocardiographic changes in patients who had recovered from COVID-19 was 44.3%. RV systolic dysfunction was identified in 31.1%, followed by ventricular dilation in 14.7% and pulmonary hypertension in 9.8%. An association was observed between SARS and RV echocardiographic changes in recovered patients during outpatient follow-up (OR: 4.96; 95% CI: 1.37-17.9; p = 0.015). An association was also demonstrated between SARS and RV dilation (p = 0.007) and between SARS and systolic dysfunction (p = 0.028). CONCLUSION: SARS is a risk factor for abnormal RV echocardiographic findings in patients recovered from COVID-19.

Three-dimensional assessment of craniofacial asymmetry in children with transverse maxillary deficiency after rapid maxillary expansion: A prospective study.

OBJECTIVE: The aim of this study was to evaluate craniofacial asymmetry in children with transverse maxillary deficiency, with or without functional unilateral posterior crossbite (UPC), before and after rapid maxillary expansion (RME). SETTING AND SAMPLE POPULATION: A sample of 51 children with cone beam computed tomography scans obtained before RME (T1) and a year after RME (T2). MATERIAL AND METHODS: This prospective study consisted of 2 groups: 25 children with functional UPC (6.77 ± 1.5 years) and 26 children without UPC (7.41 ± 1.31 years). Linear and angular measurements were obtained from zygomatic, maxilla, glenoid fossa and mandible, using original and mirrored 3D overlapped models. All right and left side comparisons in both groups and intergroups asymmetries were compared using MANOVA and t test for independent samples, respectively, statistically significant at P < .05. RESULTS: The UPC group showed no side differences, but mandibular horizontal rotation at T1, and this asymmetry was improved in T2. The non-UPC group showed at baseline significant lateral asymmetry in orbitale, position of palatine foramen, respectively, in average 2.95 mm and 1.16 mm, and 0.49 mm of average asymmetry in condylar height. The glenoid fossa was symmetric in both groups at T1 and T2. CONCLUSIONS: Children with transverse maxillary deficiency showed slight morphological asymmetry, located in the mandible position in cases of UPC, and in the orbital and maxillary regions in cases without UPC. One year after RME, patients improved their craniofacial asymmetry, with significant changes in the mandible and correction of the mandibular rotation in patients who presented UPC.

An Oral Health-Related Quality of Life Comparison Between Adults With Unilateral Cleft Lip and Palate and Class III Malocclusion.

OBJECTIVE: To compare oral health-related quality of life (OHRQoL) before treatment of adults with unilateral cleft lip and palate (UCLP) and surgical Class III malocclusion, and to consider if clefts needing different orthodontic treatment protocols could influence people's self-perception. DESIGN: Cross sectional. SETTING: Cleft Lip and Palate Center and Clinic of Orthognathic Surgery from a School of Dentistry. PARTICIPANTS: A sample of adults with repaired nonsyndromic UCLP (n = 52) which was age- and sex-matched with a noncleft Class III malocclusion sample seeking orthognathic surgery (n = 51). In turn, the cleft group was subdivided according to treatment planning into nonsurgical orthodontic and surgical orthodontic approaches. MAIN OUTCOME MEASURE: The whole sample was assessed using the short-form oral health impact profile (OHIP-14), with higher scores indicating a poorer OHRQoL. Statistical comparisons were performed with Mann-Whitney U and Kruskal-Wallis tests, and effect size. Bonferroni adjustment was used for post hoc tests (P < .017). RESULTS: The OHIP-14 scores of the UCLP and Class III groups were significantly different (P = .001, η2 = 0.108), and higher in Class III. The largest commitment was in the physical disability, physical pain, and psychological disability domains. In addition, no differences were found when the UCLP treatment planning was considered. CONCLUSION: Surgical Class III malocclusion have a poorer OHRQoL when compared to patients with UCLP, irrespective of whether they are treated surgically or orthodontically. Therefore, the greater commitment of OHRQoL appears to be influenced by the etiology of Class III, and not by treatment plan.

CD8+ tissue-resident memory T-cell development depends on infection-matching regulatory T-cell types.

Immunological memory is critical for immune protection, particularly at epithelial sites, which are under constant risk of pathogen invasions. To counter invading pathogens, CD8+ memory T cells develop at the location of infection: tissue-resident memory T cells (TRM). CD8+ T-cell responses are associated with type-1 infections and type-1 regulatory T cells (TREG) are important for CD8+ T-cell development, however, if CD8+ TRM cells develop under other infection types and require immune type-specific TREG cells is unknown. We used three distinct lung infection models, to show that type-2 helminth infection does not establish CD8+ TRM cells. Intracellular (type-1) and extracellular (type-3) infections do and rely on the recruitment of response type-matching TREG population contributing transforming growth factor-ß. Nevertheless, type-1 TREG cells remain the most important population for TRM cell development. Once established, TRM cells maintain their immune type profile. These results may have implications in the development of vaccines inducing CD8+ TRM cells.

The fellowship of regulatory and tissue-resident memory cells.

T cells located in non-lymphoid tissues have come to prominence in recent years. CD8+ tissue-resident memory (Trm) cells are important for tissue immune surveillance, provide an important line of defence against invading pathogens and show promise in cancer therapies. These cells differ in phenotype from other memory populations, are adapted to the tissue they home to where they found their cognate antigen and have different metabolic requirements for survival and activation. CD4+ Foxp3+ regulatory T (Treg) cells also consist of specialised populations, found in non-lymphoid tissues, with distinct transcriptional programmes. These cells have equally adapted to function in the tissue they made their home. Both Trm and Treg cells have functions beyond immune defence, involving tissue homeostasis, repair and turnover. They are part of a multicellular communication network. Intriguingly, occupying the same niche, Treg cells are important in the establishment of Trm cells, which may have implications to harness the immune surveillance and tissue homeostasis properties of Trm cells for future therapies.

Risk factors and prediction of acute kidney injury after liver transplantation: Logistic regression and artificial neural network approaches.

BACKGROUND: Acute kidney injury (AKI) has serious consequences on the prognosis of patients undergoing liver transplantation. Recently, artificial neural network (ANN) was reported to have better predictive ability than the classical logistic regression (LR) for this postoperative outcome. AIM: To identify the risk factors of AKI after deceased-donor liver transplantation (DDLT) and compare the prediction performance of ANN with that of LR for this complication. METHODS: Adult patients with no evidence of end-stage kidney dysfunction (KD) who underwent the first DDLT according to model for end-stage liver disease (MELD) score allocation system was evaluated. AKI was defined according to the International Club of Ascites criteria, and potential predictors of postoperative AKI were identified by LR. The prediction performance of both ANN and LR was tested. RESULTS: The incidence of AKI was 60.6% (n = 88/145) and the following predictors were identified by LR: MELD score > 25 (odds ratio [OR] = 1.999), preoperative kidney dysfunction (OR = 1.279), extended criteria donors (OR = 1.191), intraoperative arterial hypotension (OR = 1.935), intraoperative massive blood transfusion (MBT) (OR = 1.830), and postoperative serum lactate (SL) (OR = 2.001). The area under the receiver-operating characteristic curve was best for ANN (0.81, 95% confidence interval [CI]: 0.75-0.83) than for LR (0.71, 95%CI: 0.67-0.76). The root-mean-square error and mean absolute error in the ANN model were 0.47 and 0.38, respectively. CONCLUSION: The severity of liver disease, pre-existing kidney dysfunction, marginal grafts, hemodynamic instability, MBT, and SL are predictors of postoperative AKI, and ANN has better prediction performance than LR in this scenario.

Prevalence of Bruxism in Brazilian Children: A Systematic Review and Meta-Analysis.

Purpose: The prevalence of bruxism in children varies considerably. The purpose of this study was to synthesize evidence of the prevalence of bruxism in Brazilian children and consider how proportions differ between genders, assessment approaches, and geographical regions. Methods: A search was conducted using five databases and in gray literature. Two independent investigators selected the studies and extracted data. The risk of bias was assessed via the Joanna Briggs Institute tool for studies on prevalence. The certainty of the evidence was evaluated using Grading of Recommendations Assessment, Development, and Evaluation (GRADE). Results: Twenty-two cross-sectional studies were included after a two-step selection. Overall, 13,076 children from all regions of the country were assessed. The risk of bias ranged from high to low. Data were pooled in a random-effect model and resulted in an overall prevalence of sleep and awake bruxism of 25.8 percent (95 percent confidence interval [95% CI] equals 22.2 to 29.4; I2 equals 96 percent; prediction interval equals 0.07 to 0.44) and 20.1 percent (95% CI equals 18.0 to 22.3; I2 equals 30 percent; prediction interval equals 0.18 to 0.22), respectively. Subgroup and sensibility analysis showed distribution similarity between genders (P=0.96), assessment approaches (P=0.88), and geographical regions (P=0.44). Conclusions: "Possible" and "probable" sleep bruxism affects one in four Brazilian children, and there is evidence with a low level of certainty that its prevalence does not vary between genders, assessment approaches, or geographical regions. The distribution of bruxism is still an unknown subject and presumably occurs because of individual rather than regional or collective factors.

Prevalence of cleft lip and palate and associated factors in Brazil's Midwest: a single-center study.

The aim of this study was to evaluate the prevalence of cleft lip and/or palate (CL/P) and associated factors in patients treated at a referral service in Brazil's Midwest. Data were obtained from medical records on file between 2010 and 2017 for this epidemiologic and associational study. A descriptive analysis of the sociodemographic and clinical data was carried out, after which the data were analyzed using the chi-square test and Poisson regression with robust variance. A total of 1,696 medical records were eligible. The requests for rehabilitation were mainly for children in the early years of life, and were mostly for patients from low-income families in the state of Goiás. CL/P was more prevalent in its most severe morphological representation (cleft lip and palate), and the most frequently affected side was the left. Syndromic cleft was present in 4.1% of the cases, and the Pierre Robin sequence and Apert syndrome appeared more frequently. Adjusted multivariate Poisson regression showed an association between cleft palate and the presence of syndromes, since the prevalence was 2.33 times higher in this case than that of no syndrome. Cleft lip and palate were associated with males, whereas cleft palate was associated with females. This study highlights the importance of collecting and analyzing epidemiological data, managing health service planning, and allocating funds to assist cleft patients.

Deficiency in the glycosyltransferase Gcnt1 increases susceptibility to tuberculosis through a mechanism involving neutrophils.

Modulation of immunity and disease by glycans is increasingly recognized. However, how host glycosylation shapes and is shaped by tuberculosis remains poorly understood. We show that deficiency in the glucosaminyl (N-acetyl) transferase 1 (Gcnt1), a key enzyme for core-2 O-glycans biosynthesis, drives susceptibility to Mycobacterium tuberculosis infection. The increased susceptibility of Gcnt1 deficient mice was characterized by extensive lung immune pathology, mechanistically related to neutrophils. Uninfected Gcnt1 deficient mice presented bone marrow, blood and lung neutrophilia, which further increased with infection. Blood neutrophilia required Gcnt1 deficiency in the hematopoietic compartment, relating with enhanced granulopoiesis, but normal cellular egress from the bone marrow. Interestingly, for the blood neutrophilia to translate into susceptibility to M. tuberculosis infection, Gnct1 deficiency in the stroma was also necessary. Complete Gcnt1 deficiency associated with increased lung expression of the neutrophil chemoattractant CXCL2. Lastly, we demonstrate that the transcript levels of various glycosyltransferase-encoding genes were altered in whole blood of active tuberculosis patients and that sialyl Lewis x, a glycan widely present in human neutrophils, was detected in the lung of tuberculosis patients. Our findings reveal a previously unappreciated link between Gcnt1, neutrophilia and susceptibility to M. tuberculosis infection, uncovering new players balancing the immune response in tuberculosis.

Mycobacterium tuberculosis associated with severe tuberculosis evades cytosolic surveillance systems and modulates IL-1ß production.

Genetic diversity of Mycobacterium tuberculosis affects immune responses and clinical outcomes of tuberculosis (TB). However, how bacterial diversity orchestrates immune responses to direct distinct TB severities is unknown. Here we study 681 patients with pulmonary TB and show that M. tuberculosis isolates from cases with mild disease consistently induce robust cytokine responses in macrophages across multiple donors. By contrast, bacteria from patients with severe TB do not do so. Secretion of IL-1ß is a good surrogate of the differences observed, and thus to classify strains as probable drivers of different TB severities. Furthermore, we demonstrate that M. tuberculosis isolates that induce low levels of IL-1ß production can evade macrophage cytosolic surveillance systems, including cGAS and the inflammasome. Isolates exhibiting this evasion strategy carry candidate mutations, generating sigA recognition boxes or affecting components of the ESX-1 secretion system. Therefore, we provide evidence that M. tuberculosis strains manipulate host-pathogen interactions to drive variable TB severities.

Influence of music on the behavior of children during dental care / Influência da música no comportamento de crianças frente ao atendimento odontológico

ABSTRACT Objective: To evaluate the role of music as a distraction technique to minimize anxiety during dental care in children aged 7 to 9 years when submitted to local anesthetic procedure. Method: This study was carried out in the Dentistry Module of the State University of Southwestern Bahia, located in the city of Jequié, Bahia, Brazil. It was performed through the application of the Facial Image Scale, measurement of heart rate and blood pressure with the aid of the digital sphygmomanometer at two different times during pediatric dentistry care with and without the influence of classical music. Data were tabulated in the Statistical Package for Social Sciences software, version 21.0, expressed as medians and interquartile range. For inferential statistics, the Shapiro-Wilk normality test was used and the analysis of the effect of music was calculated using the paired T-tests and Wilcoxon, considering a significance level of 95%. Results: The sample consisted of 7 children, mostly male, with a median of 8 years of age. Regarding the psychophysiological variables, no significant variations were observed compared to before and after in the groups with music and without music. Conclusion: In this study, no differences were found regarding the reduction of anxiety in the group in which music was used. Additional studies with a representative sample are needed.

RESUMO Objetivo: avaliar o papel da música como técnica de distração para minimizar a ansiedade durante o atendimento odontológico em crianças de 7 a 9 anos de idade quando submetidas a procedimento anestésico local. Métodos: Este estudo foi realizado no Módulo de Odontologia da Universidade Estadual do Sudoeste da Bahia, situado na cidade de Jequié - BA. Foi realizado a partir da aplicação da Facial Image Scale, mensuração da frequência cardíaca e pressão arterial com auxílio do esfigmomanômetro digital em dois momentos distintos ao longo do atendimento em odontopediatria com e sem a influência da música clássica. Os dados foram tabulados no software Statistical Package for Social Sciences, versão 21.0, expressos como medianas e intervalo interquartílico. Para a estatística inferencial, utilizou-se o teste de normalidade Shapiro-Wilk e a análise do efeito da música foi calculada utilizando os testes T-pareado e o Wilcoxon, considerando nível de significância de 95%. Resultados: A amostra foi constituída por 7 crianças, pertencendo sua maioria ao sexo masculino, com uma mediana de 8 anos de idade. Em relação às variáveis psicofisiológicas, não foram observadas variações significativas em comparação ao antes e depois nos grupos com música e sem música. Conclusões: Neste estudo não foram encontradas diferenças no que diz respeito à redução da ansiedade no grupo em que a música foi utilizada, havendo necessidade de estudos adicionais, com amostra representativa.

Response of microbial communities colonizing salt marsh plants rhizosphere to copper oxide nanoparticles contamination and its implications for phytoremediation processes.

This study aimed to investigate Cu oxide nanoparticles (CuO NP) effect on microbial communities associated with salt marsh plants (Halimione portulacoides and Pragmites australis) rhizosphere and its implications for phytoremediation processes. Experiments were conducted, under controlled conditions, over one week. Rhizosediment soaked in the respective elutriate (a simplified natural medium) with or without plants, was doped with CuO NP or with Cu in ionic form. Microbial community in rhizosediments was characterized in terms of abundance (by DAPI) and structure (by ARISA). Metal uptake by plants was evaluated by measuring Cu in plant tissues (by atomic absorption spectroscopy). Results revealed significant metal uptake but only in plant roots, which was significantly lower (H. portulacoides) or not significant (P. australis) when the metal was in NP form. Microbial community structure was significantly changed by the treatment (absence/presence of Cu, ionic Cu or CuO NP) as showed by multivariate analysis of ARISA profiles and confirmed by analysis of similarities (Global test - one way ANOSIM). Moreover, in P. australis rhizosediments microbial abundance, bacterial richness and diversity indexes were significantly affected (increased or decreased) due to metal presence whereas in H. portulacoides rhizosediment microbial abundance showed a significant decrease, particularly when the metal was in NP form. Accordingly, Cu presence affected the response of the rhizosphere microbial community and in some cases that response was significantly different when Cu was in NP form. The response of the microbial communities to Cu NP might also contribute to the lower metal accumulation by plants when the metal was in this form. So, Cu NP may cause disturbances in ecosystems functions, ultimately affecting phytoremediation processes. These facts should be considered regarding the use of appropriate salt marshes plants to remediate moderately impacted areas such as estuaries, where NPs can be found.

Prevalence of cleft lip and palate and associated factors in Brazil's Midwest: a single-center study

Abstract: The aim of this study was to evaluate the prevalence of cleft lip and/or palate (CL/P) and associated factors in patients treated at a referral service in Brazil's Midwest. Data were obtained from medical records on file between 2010 and 2017 for this epidemiologic and associational study. A descriptive analysis of the sociodemographic and clinical data was carried out, after which the data were analyzed using the chi-square test and Poisson regression with robust variance. A total of 1,696 medical records were eligible. The requests for rehabilitation were mainly for children in the early years of life, and were mostly for patients from low-income families in the state of Goiás. CL/P was more prevalent in its most severe morphological representation (cleft lip and palate), and the most frequently affected side was the left. Syndromic cleft was present in 4.1% of the cases, and the Pierre Robin sequence and Apert syndrome appeared more frequently. Adjusted multivariate Poisson regression showed an association between cleft palate and the presence of syndromes, since the prevalence was 2.33 times higher in this case than that of no syndrome. Cleft lip and palate were associated with males, whereas cleft palate was associated with females. This study highlights the importance of collecting and analyzing epidemiological data, managing health service planning, and allocating funds to assist cleft patients.

Relação entre empregabilidade e habilidades sociais na Rede Federal de Educação Tecnológica / Relationship between employability and social skills in the federal network of technological education

O mercado de trabalho conectado, integrado e tecnológico impulsiona a busca pelo desenvolvimento de habilidades sociais para o trabalho e o potencial de empregabilidade. Nesse contexto, o objetivo é avaliar a existência de correlação entre Habilidades Sociais e Empregabilidade. A abordagem utilizada na pesquisa foi quantitativa com a análise de correlação entre as variáveis. Participaram da amostra, 260 estudantes de uma Instituição de Educação Básica, Técnica e Tecnológica. Os resultados evidenciam que existe uma correlação positiva entre o repertório Habilidades Sociais e as subescalas de Empregabilidade para a amostra geral e amostra estratificada em sexo masculino, curso superior e estudantes não trabalhadores. Destaca-se que em todas as correlações a subescala ‘otimismo’ está associada ao reportório de Habilidades Sociais. Outras correlações foram verificadas como ‘eficácia de busca’, ‘enfrentamento de dificuldades’, ‘responsabilidade’ e ‘decisão com o repertório de habilidades sociais’. Esses resultados possibilitam que diferentes instituições de ensino proponham ações voltadas para desenvolvimento de Habilidades Sociais e Empregabilidades de seus estudantes. Por fim, são apresentadas as limitações da pesquisa e sugestões para futuros trabalhos.(AU)

The connected, integrated, and technological labor market drives the search for the development of social skills for work and the potential for employability. In this context, the objective of this article is to assess the existence of a correlation between Social Skills and Employability. The approach used in the research was quantitative with the analysis of the correlation between the variables. Two hundred sixty students from a Basic, Technical, and Technological Education Institution participated in the sample. The results show a positive correlation between the Social Skills repertoire and the Employability subscales for the general sample and stratified sample in male, college, and non-working students. It is noteworthy that in all correlations, the subscale ‘optimism’ is associated with the Social Skills repertoire. Other correlations were verified, such as 'search effectiveness,’ ‘facing difficulties,’ ‘responsibility,’ and ‘decision with the social skills repertoire.’ These results make it possible for different educational institutions to propose actions aimed at developing Social Skills and Employability of their students. Finally, the limitations of the research and suggestions for future work are presented.(AU)

Avaliação da qualidade de vida relacionada à saúde bucal entre adolescentes do sudoeste baiano / Evaluation of oral health-related quality of life in adolescents in Southeast of Bahia, Brazil / Evaluación de la calidad de vida relacionada con la salud bucal de adolescentes en el suroeste de Bahia

Qualidade vida é um conceito amplo, referente à percepção subjetiva das funções físicas, psicológicas e sociais. A avaliação da qualidade de vida de adolescentes é importante para subsidiar as políticas públicas de promoção e atenção à saúde desse grupo. Nesse contexto, este artigo tem o objetivo de avaliar a qualidade de vida relacionada à saúde bucal de adolescentes de 11 a 15 anos de idade. Trata-se de um estudo do tipo Survey, com amostra aleatória de 224 adolescentes de três colégios públicos de uma cidade do Sudoeste baiano. Para a avaliação da qualidade de vida e aspectos da saúde bucal, foi aplicado o questionário CPQ 11-14. A escala de Likert de cinco pontos foi usada para quantificar as opções pelos escores. Foi feita a divisão da pontuação do CPQ por percentis para elaborar a proposta de interpretação do escore do questionário, utilizando os percentis 25% (baixo impacto), 50% (médio impacto) e 75% (elevado impacto). A mediana da idade foi de 12 anos, sendo 54,46% da amostra correspondente ao sexo feminino (122). Na comparação da média do CPQ entre as escolas de acordo com o sexo, observou-se que o feminino obteve as maiores pontuações. Na correlação entre o CPQ e faixa de idade, constatou-se que a maior mediana está concentrada na faixa etária de 11 anos. A presença de problemas orais teve domínio mais elevado. Conclui-se, a partir dos resultados, que a saúde bucal interferiu na qualidade vida dos adolescentes avaliados, sendo que a maior parte deles obteve um impacto considerado moderado.

Quality of life is a broad concept regarding the perception of physical, psychological and social functions. An assessment of the quality of life of adolescents is important to support public policies for the promotion and health care of this group. This article aims at evaluating the quality of useful life in oral health in adolescents aged 11 to 15 years. This is a research-type study with a random sample of 224 adolescents from three public schools in a city in the southwest of Bahia, Brazil. To assess quality of life and aspects of oral health, a CPQ 11-14 questionnaire was applied. A 5-point Likert scale was used to quantify options by scores. The CPQ score was divided by percentage to make a proposal to interpret the questionnaire, using the percentiles 25% (low impact), 50% (medium impact) and 75% (high impact). The median age was 12 years old, with 54.46% being female (122). When comparing the average CPQ between schools according to sex, women suffer the lowest number of scores. In the correlation between CPQ and constant age range, which is the highest median concentrated in the age group of 11 years. The presence of oral problems using higher domain. Oral health affected the quality of life of the affected adolescents, with most adolescents affected by an impact considered to be moderate.

La calidad de vida es un concepto amplio con respecto a la percepción de las funciones físicas, psicológicas y sociales. Una evaluación de la calidad de vida de los adolescentes es importante para apoyar las políticas públicas de promoción y atención médica a este grupo. Este estudio tuvo como objetivo evaluar la calidad de vida en salud bucal de adolescentes de 11 a 15 años de edad. Este es un estudio de tipo survey realizado con una muestra aleatoria de 224 adolescentes de tres escuelas públicas en una ciudad del suroeste de Bahia (Brasil). Para evaluar la calidad de vida y los aspectos de la salud oral, se aplicó el cuestionario CPQ 11-14. Se usó la escala Likert de cinco puntos para cuantificar las opciones por puntajes. El CPQ se dividió por porcentaje para hacer una propuesta de interpretación del puntaje del cuestionario utilizando los percentiles 25% (bajo impacto), 50% (mediano impacto) y 75% (alto impacto). La mediana de edad fue de 12 años, siendo el 54,46% mujeres (122). Al comparar el CPQ promedio entre las escuelas según el sexo, se observó que las mujeres obtuvieron los mayores puntajes. En la correlación entre CPQ y rango de edad, la mediana más alta estuvo concentrada en el grupo de edad de 11 años. La presencia de problemas bucales tuvo un dominio superior. Se concluye que la salud oral influyó en la calidad de vida de los adolescentes, y la mayoría de ellos se vieron afectados por un impacto considerado moderado.

A Safe and Stable Neonatal Vaccine Targeting GAPDH Confers Protection against Group B Streptococcus Infections in Adult Susceptible Mice.

Group B Streptococcus (GBS), a commensal organism, can turn into a life-threatening pathogen in neonates and elderly, or in adults with severe underlying diseases such as diabetes. We developed a vaccine targeting the GBS glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme detected at the bacterial surface, which was proven to be effective in a neonatal mouse model of infection. Since this bacterium has emerged as an important pathogen in non-pregnant adults, here we investigated whether this vaccine also confers protection in an adult susceptible and in a diabetic mouse model of infection. For immunoprotection studies, sham or immunized adult mice were infected with GBS serotype Ia and V strains, the two most prevalent serotypes isolated in adults. Sham and vaccinated mice were also rendered diabetic and infected with a serotype V GBS strain. For toxicological (pre-clinical) studies, adult mice were vaccinated three times, with three concentrations of recombinant GAPDH adjuvanted with Allydrogel, and the toxicity parameters were evaluated twenty-four hours after the last immunization. For the stability tests, the vaccine formulations were maintained at 4°C for 6 and 12 months prior immunization. The results showed that all tested doses of the vaccine, including the stability study formulations, were immunogenic and that the vaccine was innocuous. The organs (brain, blood, heart, and liver) of vaccinated susceptible or diabetic adult mice were significantly less colonized compared to those of control mice. Altogether, these results demonstrate that the GAPDH-based vaccine is safe and stable and protects susceptible and diabetic adult mice against GBS infections. It is therefore a promising candidate as a global vaccine to prevent GBS-induced neonatal and adult diseases.

Evaluation of Children's and Adolescents's Anxiety Previously Dental Treatment: A Cross-Sectional Study

Objective: To evaluate levels of anxiety in children and adolescents related to age and experience of visits to the dentist using the modified Venham Picture Test (VPTm). Material and Methods: This is a quantitative cross-sectional study with convenience sample. Children and adolescents of both sexes, subdivided into age groups of 6-9 years (A1 and A2) and 10-13 years (B1 and B2), in the waiting room of a Dental Clinic, were grouped according to the experience of visits to the dentist (have been to the dentist / have never been to the dentist). Descriptive statistics were mean, standard deviation and frequency of cases. The Kolmogorov- Smirnov (K-S) was used for normality analysis, with 95% significance level. Inferential statistics was performed using the Chi-Square and Mann-Whitney tests, with p<0.05. Results: In total, 60 children and 60 adolescents were evaluated, 65 (54.16%) males and 55 (45.83%) females. No differences were observed between genders in groups A1 and A2 (p = 0.30) and B1 and B2 (p = 0.94). The highest means of the VPTm scale were observed in groups A2 and B2 (2.87 ± 2.30 and 2.13 ± 1.29) and the lowest in groups A1 and B1 (1.13 ± 1.45 and 1.20 ± 1.29), respectively. Statistical differences were observed for the mean VPTm scores between age groups A1 and A2 (p = 0.004) and B1 and B2 (p = 0.018). Conclusion: The level of anxiety was inversely proportional to age, so younger children were more anxious; it was also observed that children and adolescents who had never been to the dentist tended to be more anxious.

Caracterização físico-química e nutricional de variedades de tomate (Lycopersicum esculentum, Mill) orgânico / Physicochemical and nutritional characterization of organic tomato varieties (Lycopersicum esculentum, Mill)

provavelmente na Região Andina, parte ocidental da América do Sul. Seus frutos são bagas carnosas, suculentas, variando em aspecto, tamanho e peso, dependendo do cultivar e do manejo adotado. Este projeto procurou estudar a composição físico-química e nutricional de sete variedades de tomate obtidas sob cultivo orgânico de produção. Os resultados apresentaram os seguintes limites: massa variou entre 17 e 143g, o pH de 3,99 a 4,88, sólidos solúveis totais de 3,83 a 4,60 ºBrix, acidez total titulável de 0,28 a 0,36%, relação SST/ATT de 11,2 a 13,9, umidade de 88,8 a 96,2%, cinzas de 0,44 a 0,97%, proteínas de 0,25 a 0,94%, lipídeos de 0,12 a 0,90%, carboidratos de 2,02 a 8,70% e VET de 15,9 a 42,5 kcal. Cada amostra foi analisada em triplicata e apresentou entre baixa a média dispersão, mas com diferenças entre cultivares que podem ser atribuídas à genética e as condições edafoclimáticas. Com os resultados obtidos, concluiu-se que, além de terem sido obtidas sob cultivo orgânico, o que já confere vantagem por não possuírem resíduos químicos, os cultivares apresentaram alta qualidade do ponto de vista físico-químico e nutricional.(AU)

The tomatoes probably have their origin in the Andean Region, western part of South America. Its berries are fleshy, succulent, varying in appearance, size and weight, depending on the cultivar and management adopted. This project sought to study the physical and chemical composition and nutritional seven tomato varieties produced under organic crop production. The results showed the following limits: mass ranged between 17 and 143g, the pH from 3.99 to 4.88, soluble solids from 3.83 to 4.60 ºBrix, titratable acidity from 0.28 to 0.36%, TSS / TTA ratio from 11.2 to 13.9 ºBrix/%, 88.8 to 96.2 moisture%, ash 0.44 to 0.97%, protein 0.25 to 0.94%, lipid 0, 12 to 0.90%, carbohydrates from 2.02 to 8.70% and from 15.9 to 42.5 VET kcal. Each sample was analyzed in triplicate and showed low and medium dispersion, but with differences between cultivars that can be attributed to genetics and the soil and climatic conditions. With these results, it was concluded that in addition to having been obtained under organic cultivation, which already gives advantage by not having chemical residues, the cultivars showed high quality in terms of physical-chemical and nutritional.