Quantification of maximal power output in well-trained cyclists.

This study aimed to compare mechanical variables derived from torque-cadence and power-cadence profiles established from different cycle ergometer modes (isoinertial and isokinetic) and modelling procedures (second- and third-order polynomials), whilst employing a novel method to validate the theoretical maximal power output (Pmax). Nineteen well-trained cyclists (n = 12 males) completed two experimental sessions comprising six, 6-s maximal isoinertial or isokinetic cycling sprints. Maximal pedal strokes were extracted to construct power-cadence relationships using second- and third-order polynomials. A 6-s sprint at the optimal cadence (Fopt) or optimal resistance (Topt) was performed to assess construct validity of Pmax. No differences were found in the mechanical parameters when derived from isokinetic (Pmax = 1311 ± 415, Fopt = 118 ± 12) or isoinertial modes (Pmax = 1320 ± 421, Fopt = 116 ± 19). However, R2 improved (P < 0.02) when derived from isoinertial sprints. Third-order polynomial modelling improved goodness of fit values (Standard Error, adjusted R2), but derived similar mechanical parameters. Finally, peak power output during the optimised sprint did not significantly differ from the theoretical Pmax in both cycling modes, thus providing construct validity. The most accurate P-C profile can be derived from isoinertial cycling sprints, modelled using third-order polynomial equations.

Quantifying the Activity Profile of Female Beach Volleyball Tournament Match-Play.

Effective time-motion analysis can provide information directly applicable to the design of physical conditioning and testing programmes. The aim of the present study was to determine the external output of female beach volleyball players during tournament match-play and to assess the effect of competition level, margin of score differential and alterations of external output within matches. The external output profile of ten adult level (age 27 ± 3 y) and ten under 23 ('U23'; age 19 ± 2 y) female beach volleyball players were determined using GPS technology (10 Hz) during 60 matches (n = 50 first and second sets and 20 third sets files) played during their respective Australian Beach Volleyball Championship tournaments. Comparisons between groups and the influence of contextual factors on the external output within matches were analysed using linear mixed models. Adult beach volleyball players covered a greater relative distance (i.e., m·min-1) in speed zone 2 (1.0-1.99 m·s-1; 42.0% of total relative distance) and zone 3 (≥2.0 m·s-1; 10.9% of total relative distance) compared to U23 players. Relative distance, mean acceleration/deceleration and the relative distance covered in acceleration zone 2 and deceleration zone 2 and 3 was greater in set 1 compared to set 2. Sets that were decided by smaller score margins (<6-point score differential) were comprised of a greater relative distance, peak speed, greater mean acceleration and deceleration output and a greater relative distance in speed zone 1 and 3 compared to sets decided by larger score margins (>5-point score differential). The findings from this study suggest that there are contextual factors that influence the speed and acceleration/deceleration profile of female beach volleyball players such as tournament level, score margin and set-to-set variations that may have implications for the physiological and mechanical requirements of female players preparing for competition.

Muscle Damage and Metabolic Responses to Repeated-Sprint Running With and Without Deceleration.

Minahan, CL, Poke, DP, Morrison, J, and Bellinger, PM. Muscle damage and metabolic responses to repeated-sprint running with and without deceleration. J Strength Cond Res 34(12): 3423-3430, 2020-This study aimed to determine whether repeated-sprint running with deceleration aggravates markers of muscle damage or delays the recovery of performance compared with repeated-sprint running without deceleration. Fourteen male team-sport athletes performed 2 randomly ordered testing sessions on a nonmotorized treadmill with one session requiring subjects to decelerate (TMd) within 4 seconds before stopping or immediately step to the side of the treadmill belt at the completion of each sprint (TMa). Peak and mean velocities, speed decrement, blood lactate concentrations, and oxygen uptake were monitored during the repeated-sprint running protocols. Countermovement vertical jump (CMJ) performance, perceived muscle soreness, sit-and-reach flexibility, plasma creatine kinase (CK), lactate dehydrogenase (LDH), and myoglobin (Mb) concentrations were quantified immediately before and after and 45 minutes, 24 and 48 hours after repeated-sprint running protocols. Although muscle damage was indicated by increases in CK, LDH, and Mb (p ≤ 0.05) in both groups, there was no significant effect of condition (TMa vs. TMd) on any of the measured performance or physiological variables (p > 0.05). The present study indicated that the removal of deceleration from repeated-sprint running on a nonmotorized treadmill has no effect on metabolism or performance during or after repeated-sprint running or markers of muscle damage.

ß-Alanine supplementation for athletic performance: an update.

ß-alanine supplementation has become a common practice among competitive athletes participating in a range of different sports. Although the mechanism by which chronic ß-alanine supplementation could have an ergogenic effect is widely debated, the popular view is that ß-alanine supplementation augments intramuscular carnosine content, leading to an increase in muscle buffer capacity, a delay in the onset of muscular fatigue, and a facilitated recovery during repeated bouts of high-intensity exercise. ß-alanine supplementation appears to be most effective for exercise tasks that rely heavily on ATP synthesis from anaerobic glycolysis. However, research investigating its efficacy as an ergogenic aid remains equivocal, making it difficult to draw conclusions as to its effectiveness for training and competition. The aim of this review was to update, summarize, and critically evaluate the findings associated with ß-alanine supplementation and exercise performance with the most recent research available to allow the development of practical recommendations for coaches and athletes. A critical review of the literature reveals that when significant ergogenic effects have been found, they have been generally shown in untrained individuals performing exercise bouts under laboratory conditions. The body of scientific data available concerning highly trained athletes performing single competition-like exercise tasks indicates that this type of population receives modest but potentially worthwhile performance benefits from ß-alanine supplementation. Recent data indicate that athletes may not only be using ß-alanine supplementation to enhance sports performance but also as a training aid to augment bouts of high-intensity training. ß-alanine supplementation has also been shown to increase resistance training performance and training volume in team-sport athletes, which may allow for greater overload and superior adaptations compared with training alone. The ergogenic potential of ß-alanine supplementation for elite athletes performing repeated high-intensity exercise bouts, either during training or during competition in sports which require repeated maximal efforts (e.g., rugby and soccer), needs scientific confirmation.

The effect of beta-alanine supplementation on isokinetic force and cycling performance in highly trained cyclists.

Beta-alanine may benefit short-duration, high-intensity exercise performance. The aim of this randomized double-blind placebo-controlled study was to examine the effects of beta-alanine supplementation on aspects of muscular performance in highly trained cyclists. Sixteen highly trained cyclists (mean ± SD; age = 24 ± 7 yr; mass = 70 ± 7 kg; VO2max = 67 ± 4 ml · kg(-1) · min(-1)) supplemented with either beta-alanine (n = 8, 65 mg · kg - 1BM) or a placebo (n = 8; dextrose monohydrate) over 4 weeks. Pre- and postsupplementation cyclists performed a 4-minute maximal cycling test to measure average power and 30 reciprocal maximal isokinetic knee contractions at a fixed angular velocity of 180° · sec(-1) to measure average power/repetition, total work done (TWD), and fatigue index (%). Blood pH, lactate (La-) and bicarbonate (HCO3-) concentrations were measured pre- and postisokinetic testing at baseline and following the supplementation period. Beta-alanine supplementation was 44% likely to increase average power output during the 4-minute cycling time trial when compared with the placebo, although this was not statistically significant (p = .25). Isokinetic average power/repetition was significantly increased post beta-alanine supplementation compared with placebo (beta-alanine: 6.8 ± 9.9 W, placebo: -4.3 ± 9.5 W, p = .04, 85% likely benefit), while fatigue index was significantly reduced (p = .03, 95% likely benefit). TWD was 89% likely to be improved following beta-alanine supplementation; however, this was not statistically significant (p = .09). There were no significant differences in blood pH, lactate, and HCO3- between groups (p > .05). Four weeks of beta-alanine supplementation resulted in worthwhile changes in time-trial performance and short-duration muscular force production in highly trained cyclists.

The Relevance of Muscle Fiber Type to Physical Characteristics and Performance in Team-Sport Athletes.

PURPOSE: The aim of this systematic review was to (1) determine the muscle fiber-type composition (or muscle fiber typology [MFT]) of team-sport athletes and (2) examine associations between MFT and the physical characteristics and performance tasks in team-sport athletes. METHODS: Searches were conducted across numerous databases-PubMed, SPORTDiscus, MEDLINE, and Google Scholar-using consistent search terms. Studies were included if they examined the MFT of team-sport athletes. Included studies underwent critical appraisal using the McMasters University critical appraisal tool for quantitative research. RESULTS: A total of 10 studies were included in the present review, wherein the MFT of athletes was measured from 5 different team sports (soccer, rugby union, rugby league, handball, and volleyball). There was large variability in the MFT of team-sport athletes both within (up to 27.5%) and between sports (24.0% relative difference). Male football players with a higher proportion of type II fibers had faster 10- and 30-m sprint times, achieved a greater total distance sprinting (distance at >6.67 m·s-1), and a greater peak 1-minute sprint distance. CONCLUSIONS: MFT varies considerably between athletes both within and between different team sports. The results from some studies suggest that variation in MFT is associated with high-intensity running performance in a football match, as well as 10- and 30-m sprint times. Further experimental studies should focus on how determination of the MFT of team-sport athletes could be utilized to influence talent identification, team selection, and the individualization of training.

Match Running Performance in Australian Football Is Related to Muscle Fiber Typology.

PURPOSE: To examine the association between muscle fiber typology and match running performance in professional Australian football (AF) athletes. METHODS: An observational time-motion analysis was performed on 23 professional AF athletes during 224 games throughout the 2020 competitive season. Athletes were categorized by position as hybrid, small, or tall. Athlete running performance was measured using Global Navigation Satellite System devices. Mean total match running performance and maximal mean intensity values were calculated for moving mean durations between 1 and 10 minutes for speed (in meters per minute), high-speed-running distance (HSR, >4.17 m·s-1), and acceleration (in meters per second squared), while intercept and slopes were calculated using power law. Carnosine content was quantified by proton magnetic resonance spectroscopy in the gastrocnemius and soleus and expressed as a carnosine aggregate z score (CAZ score) to estimate muscle fiber typology. Mixed linear models were used to determine the association between CAZ score and running performance. RESULTS: The mean (range) CAZ score was -0.60 (-1.89 to 1.25), indicating that most athletes possessed a greater estimated proportion of type I muscle fibers. A greater estimated proportion of type I fibers (ie, lower CAZ score) was associated with a larger accumulation of HSR (>4.17 m·s-1) and an increased ability to maintain HSR as the peak period duration increased. CONCLUSION: AF athletes with a greater estimated proportion of type I muscle fibers were associated with a greater capacity to accumulate distance running at high speeds, as well as a greater capacity to maintain higher output of HSR running during peak periods as duration increases.

No Influence of Prematch Subjective Wellness Ratings on External Load During Elite Australian Football Match Play.

PURPOSE: To determine whether there is an association between external match load and contextual factors on subjective wellness in the days before and after Australian Rules football match play. METHODS: A total of 34 elite male Australian football players completed a subjective wellness questionnaire in the days leading into the match (-3, -2, and -1 d), the day of (match day), and the days after each match (+3, +2, and +1 d). Players subjectively rated each item (mood, energy, stress, leg heaviness, muscle soreness, sleep quality, hours slept, and total wellness [a sum of the total response score]) on a visual analog scale ranging from 1 to 10, with 1 representing the negative end of the continuum. External load during competitive matches was quantified using accelerometer-derived PlayerLoad, and running activity was quantified using global positioning system technology across 2 competitive seasons. The relationships between perceptions of wellness (within-individual z score), external match load, and contextual factors (match result, match location, and between-matches recovery duration) were analyzed using linear mixed models. RESULTS: Mixed-effect linear models revealed trivial effects of match-day wellness z score on subsequent external match load metrics. Match result (win) and PlayerLoad in the anteroposterior vector (au·min-1) were associated with an increased (estimate ± SE: 0.30 ± 0.13 z score) and reduced subjective wellness (-0.15 ± 0.06 z score), respectively. CONCLUSION: The results of this study suggest that prematch perceived wellness does not relate to external match load in elite Australian football players. The between-matches microcycle length appears to be sufficient to restore perceived wellness to values that do not affect the subsequent external match loads.

Overreaching Attenuates Training-induced Improvements in Muscle Oxidative Capacity.

INTRODUCTION: The aim of this study was to determine whether muscle oxidative capacity is influenced by alterations in training volume in middle-distance runners. METHODS: Twenty-four highly trained middle-distance runners (n = 16 males; V˙O2peak = 73.3(4.3) mL·kg·min; n = 8 females, V˙O2peak = 63.2(3.4) mL·kg·min) completed 3 wk of normal training (NormTr), 3 wk of high-volume training (HVTr; a 10%, 20%, and 30% increase in training volume during each successive week from NormTr), and a 1-wk taper (TapTr; 55% exponential reduction in training volume from HVTr week 3). Before and immediately after each training period, the rate of recovery of muscle oxygen consumption (mV˙O2) of the gastrocnemius medialis was measured using near-infrared spectroscopy, with the rate constant indicating muscle oxidative capacity. Time to exhaustion (TTE) and V˙O2peak were determined during a maximal incremental treadmill test. RESULTS: Twelve subjects were classified as being functionally overreached (FOR) after HVTr (decreased running TTE and high perceived fatigue), whereas the other 12 subjects were classified as acutely fatigued (AF; no decrease in running TTE). The AF group demonstrated a significant increase in muscle oxidative capacity after HVTr (rate constant: 15.1% ± 9.7% min; P = 0.009), with no further improvement after TapTr, whereas there was no change in muscle oxidative capacity for FOR at any time point (P > 0.05). Compared with the FOR group, the AF group had substantially larger improvements in TTE from pre-HVTr to post-TapTr (FOR, 8.8% ± 3.7%; AF, 3.2% ± 3.0%; P = 0.04). CONCLUSION: The present study was able to demonstrate that muscle oxidative capacity was increased in response to a period of HVTr, but only in runners who did not develop FOR. Furthermore, runners who did not develop FOR had substantially larger performance improvements after a taper period.

Reliability of salivary cortisol and immunoglobulin-A measurements from the IPRO® before and after sprint cycling exercise.

BACKGROUND: The purpose of this study was to evaluate the inter-day reliability of the IPRO method for determining resting and postexercise salivary cortisol (sCort) and rate of salivary Immunoglobulin-A (sIgA) secretion. METHODS: Fourteen males (32±11 years) performed two trials (T1 and T2) separated by 7 d, comprising saliva sampling before and 15 min after completion of two, 30-s Wingate Anaerobic Tests separated by 3.5 min (2 x WAnT). sCort increased after the 2 x WAnT in both trials (T1: P<0.001; T2: P<0.001), whereas rate of sIgA secretion decreased in both trials (T1: P=0.002; T2: P=0.002). RESULTS: The intraclass correlation coefficients for resting and postexercise sCort and rate of sIgA secretion ranged from 0.96-0.99. Mean reliability, expressed as the coefficient of variation (%) and the typical error of measurement over the two trials were resting sCort 9.4%, 0.14 ng·mL-1 (9% CI: 0.1-0.2), postexercise sCort 11.9%, 0.44 ng·mL-1 (9% CI: 0.3-0.7), resting rate of sIgA secretion 7.4%, 85.5 µg·mL-1·min-1 (9% CI: 65.2-127.1) and postexercise rate of sIgA secretion 10.5%, 82.9 µg·mL-1·min-1 (9% CI: 63.2-123.1). CONCLUSIONS: The IPRO is a reliable method for determining sCort and rate of sIgA secretion at rest and after sprint-cycling exercise.

Additive Benefits of ß-Alanine Supplementation and Sprint-Interval Training.

PURPOSE: The present study investigated the effects of ß-alanine supplementation only, and in combination with sprint-interval training (SIT), on training intensity, and energy provision and performance during exhaustive supramaximal-intensity cycling and a 4- and 10-km time trial (TT). METHODS: Fourteen trained cyclists (V˙O2max = 4.5 ± 0.6 L·min) participated in this placebo-controlled, double-blind study. Subjects performed a supramaximal cycling test to exhaustion (equivalent to 120% V˙O2max) and a 4- and 10-km TT and 4 × 1-km sprints at three time points: before and after 28 d of supplementation loading (6.4 g·d) with ß-alanine (n = 7) or a placebo (n = 7), and after a 5-wk supervised, SIT program performed twice weekly (repeated 1-km cycling sprints) while maintaining supplementation with ß-alanine (1.2 g·d) or a placebo. RESULTS: After the loading period, sprints 3 and 4 of the 4 × 1-km sprint intervals were improved with ß-alanine supplementation (4.5% ± 3.4% and 7.0% ± 4.0%; P < 0.05, respectively). After 5 wk of SIT, training intensity increased in both groups but the change was greater with ß-alanine supplementation (9.9% ± 5.0% vs 4.9% ± 5.0%; P = 0.04). ß-alanine supplementation also improved supramaximal cycling time to exhaustion to a greater extent than placebo (14.9% ± 9.2% vs 9.0% ± 6.9%; P = 0.04), whereas 4- and 10-km TT performance improved to a similar magnitude in both groups. After SIT, ß-alanine also increased anaerobic capacity (5.5% ± 4.2%; P = 0.04), whereas V˙O2peak increased similarly in each group (3.1% ± 2.9% vs 3.5% ± 2.9%; P < 0.05). CONCLUSIONS: These findings indicate that ß-alanine supplementation enhances training intensity during SIT and provides additional benefits to exhaustive supramaximal cycling compared with SIT alone.

Performance effects of acute ß-alanine induced paresthesia in competitive cyclists.

ß-alanine is a common ingredient in supplements consumed by athletes. Indeed, athletes may believe that the ß-alanine induced paresthesia, experienced shortly after ingestion, is associated with its ergogenic effect despite no scientific mechanism supporting this notion. The present study examined changes in cycling performance under conditions of ß-alanine induced paresthesia. Eight competitive cyclists (VO2max = 61.8 ± 4.2 mL·kg·min(-1)) performed three practices, one baseline and four experimental trials. The experimental trials comprised a 1-km cycling time trial under four conditions with varying information (i.e., athlete informed ß-alanine or placebo) and supplement content (athlete received ß-alanine or placebo) delivered to the cyclist: informed ß-alanine/received ß-alanine, informed placebo/received ß-alanine, informed ß-alanine/received placebo and informed placebo/received placebo. Questionnaires were undertaken exploring the cyclists' experience of the effects of the experimental conditions. A possibly likely increase in mean power was associated with conditions in which ß-alanine was administered (±95% CL: 2.2% ± 4.0%), but these results were inconclusive for performance enhancement (p = 0.32, effect size = 0.18, smallest worthwhile change = 56% beneficial). A possibly harmful effect was observed when cyclists were correctly informed that they had ingested a placebo (-1.0% ± 1.9%). Questionnaire data suggested that ß-alanine ingestion resulted in evident sensory side effects and six cyclists reported placebo effects. Acute ingestion of ß-alanine is not associated with improved 1-km TT performance in competitive cyclists. These findings are in contrast to the athlete's "belief" as cyclists reported improved energy and the ability to sustain a higher power output under conditions of ß-alanine induced paresthesia.

The effect of ß-alanine supplementation on cycling time trials of different length.

The varying results reported in response to ß-alanine supplementation may be related to the duration and nature of the exercise protocol employed. We investigated the effects of ß-alanine supplementation on a wide range of cycling performance tests in order to produce a clear concise set of criteria for its efficacy. Fourteen trained cyclists (Age = 24.8 ± 6.7 years; VO2max = 65.4 ± 10.2 mL·kg·min(-1)) participated in this placebo-controlled, double-blind study. Prior to supplementation, subjects completed two (familiarization and baseline) supramaximal cycling bouts until exhaustion (120% pre-supplementation VO2max) and two 1-, 4- and 10-km cycling time trial (TT). Subjects then supplemented orally for 4 weeks with 6.4 g/d placebo or ß-alanine and repeated the battery of performance tests. Blood lactate was measured pre-exercise, post-exercise and 5  min post-exercise. ß-alanine supplementation elicited significant increases in time to exhaustion (TTE) (17.6 ± 11.5 s; p = 0.013, effect compared with placebo) and was likely to be beneficial to 4-km TT performance time (-7.8 ± 8.1 s; 94% likelihood), despite not being statistically different (p = 0.060). Performance times in the 1- and 10-km TT were not affected by treatment. For the highly trained cyclists in the current study, ß-alanine supplementation significantly extended supramaximal cycling TTE and may have provided a worthwhile improvement to 4-km TT performance. However, 1- and 10-km cycling TT performance appears to be unaffected by ß-alanine supplementation.

Metabolic consequences of ß-alanine supplementation during exhaustive supramaximal cycling and 4000-m time-trial performance.

The present study investigated the effects of ß-alanine supplementation on the resultant blood acidosis, lactate accumulation, and energy provision during supramaximal-intensity cycling, as well as the aerobic and anaerobic contribution to power output during a 4000-m cycling time trial (TT). Seventeen trained cyclists (maximal oxygen uptake = 4.47 ± 0.55 L·min(-1)) were administered 6.4 g of ß-alanine (n = 9) or placebo (n = 8) daily for 4 weeks. Participants performed a supramaximal cycling test to exhaustion (equivalent to 120% maximal oxygen uptake) before (PreExh) and after (PostExh) the 4-week supplementation period, as well as an additional postsupplementation supramaximal cycling test identical in duration and power output to PreExh (PostMatch). Anaerobic capacity was quantified and blood pH, lactate, and bicarbonate concentrations were measured pre-, immediately post-, and 5 min postexercise. Subjects also performed a 4000-m cycling TT before and after supplementation while the aerobic and anaerobic contributions to power output were quantified. ß-Alanine supplementation increased time to exhaustion (+12.8 ± 8.2 s; P = 0.041) and anaerobic capacity (+1.1 ± 0.7 kJ; P = 0.048) in PostExh compared with PreExh. Performance time in the 4000-m TT was reduced following ß-alanine supplementation (-6.3 ± 4.6 s; P = 0.034) and the mean anaerobic power output was likely to be greater (+6.2 ± 4.5 W; P = 0.035). ß-Alanine supplementation increased time to exhaustion concomitant with an augmented anaerobic capacity during supramaximal intensity cycling, which was also mirrored by a meaningful increase in the anaerobic contribution to power output during a 4000-m cycling TT, resulting in an enhanced overall performance.

Anaerobic Energy Production During Sprint Paddling in Junior Competitive and Recreational Surfers.

This study compared determinants of a 30-s all-out paddling effort (30-s sprint-paddling test) between junior surfboard riders (surfers) of varying ability. Eight competitive (COMP) and 8 recreational (REC) junior male surfers performed a 30-s sprint-paddling test for the determination of peak sprint power and accumulated O2 deficit. Surfers also performed an incremental-paddling test for the determination of the O2 uptake-power output relationship that was subsequently used to calculate the accumulated O2 deficit for the 30-s sprint-paddling test. During the 30-s sprint-paddling test, peak sprint power (404 ± 98 vs 292 ± 56 W, respectively, P = .01) and the accumulated O2 deficit (1.60 ± 0.31 vs 1.14 ± 0.38 L, respectively, P = .02) were greater in COMP than in REC surfers, whereas peak O2 uptake measured during the incremental-paddling test was not different (2.7 ± 0.1 vs 2.5 ± 0.2 L/min, respectively, P = .11). The higher peak sprint power and larger accumulated O2 deficit observed in COMP than in REC surfers during a 30-s sprint paddling test suggest that surfing promotes development of the anaerobic energy systems. Furthermore, peak sprint power determined during 30 s of sprint paddling may be considered a sensitive measure of surfing ability or experience in junior male surfers.

Effect of combined ß-alanine and sodium bicarbonate supplementation on cycling performance.

PURPOSE: The purpose of this study was to investigate the effects of 28 d of ß-alanine supplementation on 4-min cycling time trial performance and to determine whether there was an additive effect of combined ß-alanine and sodium bicarbonate (NaHCO3) supplementation on high-intensity cycling performance. METHODS: Fourteen highly trained cyclists (mean ± SD: age = 25.4 ± 7.2 yr, mass = 71.1 ± 7.1 kg, V˙O(2max) = 66.6 ± 5.7 mL·kg·min) supplemented for 28 d with ß-alanine (65 mg·kg body mass each day) or placebo. A maximal 4-min bout of cycling was performed before supplementation (baseline) and twice after supplementation: after ingestion of NaHCO3 (300 mg·kg body mass) and ingestion of a placebo using a randomized crossover design with 2 d between trials. Blood pH and HCO3 concentration were determined before loading (postsupplementation trials) and at pretest and posttest. RESULTS: In the acute NaHCO3 loading trials, blood pH and HCO3 were elevated from before loading to pretest, and the magnitude of the change in HCO3 from pretest to posttest was significantly greater compared with the acute placebo loading trial (P < 0.001). Average power output in the 4-min cycling performance trial was increased in placebo + NaHCO3 (+3.1% ± 1.8%) and ß-alanine + NaHCO3 (+3.3% ± 3.0%) compared with baseline (P < 0.05). ß-alanine + placebo did not significantly improve average power output compared with baseline (+1.6% ± 1.7%, P = 0.20); however, magnitude-based inferences demonstrated that ß-alanine + placebo was associated with a 37% likelihood of producing average power improvements. CONCLUSIONS: In trained cyclists, ß-alanine supplementation did not significantly improve 4-min cycling performance; however, there may be a small meaningful improvement in performance. Acute NaHCO3 supplementation significantly improved 4-min cycling performance. There seemed to be a minimal additive effect of combined ß-alanine and NaHCO3 supplementation.