RESUMO
Wet weight of rat cerebral cortex was increased by exposure to an enriched environment, as compared with standard colony or impoverished conditions. Dry weights and wet weights were compared and both yielded identical percentage differences between brains of animals experiencing enrichment and those experiencing impoverishment.
Assuntos
Córtex Cerebral/anatomia & histologia , Meio Ambiente , Tamanho do Órgão , Animais , Masculino , RatosRESUMO
Mice were trained in a passive (foot shock)avoidance task. When administered after training, the stimulants caffeine or nicotine blocked amnesia for the task that had been produced by injections of the protein synthesis inhibitor anisomycin given prior to training. With foot shock at a higher intensity, anisomycin did not produce amnesia by itself, but the administration of the depressants chloral hydrate or sodium phenobarbital after training did cause amnesia. Stimulants and depressants did not have an appreciable influence on the overall degree of protein synthesis inhibition produced by anisomycin. The results support the hypothesis that arousal after training is an important factor in the conversion of short-term to long-term memory.
Assuntos
Anisomicina/farmacologia , Aprendizagem da Esquiva/efeitos dos fármacos , Encéfalo/efeitos dos fármacos , Memória/efeitos dos fármacos , Pirrolidinas/farmacologia , Animais , Cafeína/farmacologia , Hidrato de Cloral/farmacologia , Interações Medicamentosas , Masculino , Camundongos , Proteínas do Tecido Nervoso/biossíntese , Nicotina/farmacologia , Fenobarbital/farmacologiaRESUMO
High-affinity, specific binding of radiolabeled alpha-bungarotoxin to particulate fractions derived from rat brain shows saturability (Bmax approximately 37fmol/mg, KDapp = 1.7 nM) and insenstivity to ionic strength, and is essentially irreversibel (Kon = 5 . 10(6) min-1 . mol-1; Kdisplacement = 1.9 . 10(-4) min-1, tau1/2 = 62 h). Subcellular distribution of specific sites is consistent with their location on synaptic junctional complex and post-synaptic membranes. These membrane-bound binding sites exhibit unique sensitivity to cholinergic ligands; pretreatment of membranes with cholinerin binding sites to a high affinity form toward agonist. The effect is most marked for the natural agonist, acetylcholine. These results strongly support the notion that the entity under study is an authentic nicotinic acetylcholine receptor.
Assuntos
Encéfalo/metabolismo , Bungarotoxinas/metabolismo , Receptores Colinérgicos/metabolismo , Receptores Nicotínicos/metabolismo , Animais , Ligação Competitiva , Núcleo Celular/metabolismo , Embrião de Galinha , Cinética , Microssomos/metabolismo , Mitocôndrias/metabolismo , Ratos , Receptores Nicotínicos/isolamento & purificaçãoRESUMO
The purpose of this study was to investigate in more detail the characteristics of the age-related extension of the retrograde amnesia gradient previously demonstrated in a passive avoidance task [6]. In Experiment 1, it was found that while 2-3 month old mice were susceptible to the amnesic effects of anisomycin (ANI) only when given prior to 15 min post-training, memory of 14-16 month old mice was susceptible to disruption when ANI was given as late as 20 min post-training, and retention of 17-20 month old mice was impaired when ANI was injected even as late as 30 min after training. Experiment 2 examined whether the age-related change in susceptibility to the effects of ANI could be ameliorated by chronic pretreatment with a choline-enriched diet. Results showed that ANI injected 20 min after training did not induce amnesia in choline treated mice (14.5 month old), but did induce amnesia when injected 15 min post training. Subsequent assay of choline acetyltransferase (ChAT) and tyrosine hydroxylase (TH) activity showed that choline treatment significantly reduced ChAT activity but did not affect TH activity. It appears that dietary choline treatment can render new long-term memories less susceptible to disruption following training.
Assuntos
Envelhecimento , Química Encefálica/efeitos dos fármacos , Colina/farmacologia , Memória/efeitos dos fármacos , Animais , Anisomicina/farmacologia , Aprendizagem da Esquiva/efeitos dos fármacos , Córtex Cerebral/enzimologia , Colina O-Acetiltransferase/análise , Corpo Estriado/enzimologia , Dieta , Hipocampo/enzimologia , Masculino , Camundongos , Camundongos Endogâmicos , Atividade Motora/efeitos dos fármacos , Tirosina 3-Mono-Oxigenase/análiseRESUMO
The extent to which protein synthesis is involved in working memory was investigated with the protein synthesis inhibitor anisomycin (ANI). Rats were trained to perform accurately on a 12-arm radial maze when delays of 240 min were interposed between choice 6 and choice 7. Bilateral hippocampal cannulas were then implanted. Accuracy on choices 7-12 was studied when ANI or saline was injected either 30 min before choice 1 or 5-10 min after choice 6 (Experiment 1). Pretrial injection of ANI significantly impaired performance following the 240-min delay, whereas ANI injected during the delay had no such effect. In Experiments 2 and 3, the ANI-induced amnesia was replicated, and the temporal course of development of the amnesia was determined. Pretrial administration of ANI did not significantly affect retention after a 2-min delay but did produce amnesia after delays of 15 min or longer. These data suggest that protein synthesis is important for the formation of temporary memories, provided the retention interval is long enough. It is suggested that working memory includes both short-term and long-term components. Protein synthesis appears to be important for formation of the long-term component, but not the short-term component, of working memory.
Assuntos
Anisomicina/farmacologia , Hipocampo/efeitos dos fármacos , Memória/efeitos dos fármacos , Pirrolidinas/farmacologia , Animais , Hipocampo/fisiologia , Masculino , Memória/fisiologia , Proteínas do Tecido Nervoso/biossíntese , Ratos , Fatores de TempoRESUMO
Previous experiments suggested that working memory of rats trained on a radial maze can be discussed in terms of its short- and long-term temporal components. For example, in Mizumori, Channon, Rosenzweig, and Bennett's (1985) study, long-term working memory was found to be susceptible to disruption by the protein synthesis inhibitor anisomycin (ANI). In Experiment 1 of this report, we examined the neuropharmacological nature of short-term working memory of rats trained to retrieve food from all arms of a 12-arm radial maze. Delay intervals of varying length were placed between Choices 6 and 7. Lanthanum (LaCl3) and glutamate (GLU) injected bilaterally into the hippocampus effectively impaired retention over short delay intervals, which suggests a possible role for calcium and/or potassium and for glutamate in working memory. However, another equally likely explanation for the amnesic effects of LaCl3 and GLU is that these drugs impaired reference memory. To test more directly the hypothesis that LaCl3, GLU, or ANI might differentially affect working and reference memory, we tested the effects of these drugs on performance of rats trained to retrieve food from only 8 arms of the 12-arm maze in Experiment 2. The remaining 4 arms were never baited, in order to test reference memory function. We predicted that rats would make errors only in baited arms (i.e., errors of working memory). Instead, results of Experiment 2 showed that LaCl3, GLU, or ANI injection produced errors in unbaited arms even before a 120-min delay. If rats were injected with LaCl3 or GLU, baited-arm errors were observed only after the delay period.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Memória de Curto Prazo/fisiologia , Memória/fisiologia , Animais , Anisomicina/farmacologia , Glutamatos/farmacologia , Ácido Glutâmico , Lantânio/farmacologia , Memória/efeitos dos fármacos , Memória de Curto Prazo/efeitos dos fármacos , Ratos , Ratos Endogâmicos , Retenção Psicológica/efeitos dos fármacosRESUMO
Stages of formation of memory and the roles of different forebrain structures in memory formation were investigated by injecting various agents into the brains of chicks close to the time of peck-avoidance training. With L-glutamate injected bilaterally into the hyperstriatum 5 min pretraining, retention was good 1 min posttraining but significantly impaired at 5 min and each subsequent time point from 10 min to 24 hr. With ouabain, retention declined more slowly, showing significant impairment at 15 min and thereafter. With any of three protein synthesis inhibitors (anisomycin, cycloheximide, or emetine), retention was still good 60 min posttraining but significantly impaired at 90 min. The three time courses of decline of retention are consistent with hypotheses of three sequentially dependent stages of memory formation. Glutamate, ouabain, and emetine were found to affect only a restricted volume of tissue. Any of these three agents induced amnesia when injected into the left (but not the right) medial hyperstriatum ventrale or into the right (but not the left) lateral neostriatum; so it appears that both structures are required for formation of memory. Agents that are specific for a presumed stage of memory formation and whose action is restricted spatially should help reveal the roles of different brain structures in different stages of memory formation.
Assuntos
Encéfalo/fisiologia , Galinhas/fisiologia , Memória/fisiologia , Animais , Anisomicina/farmacologia , Mapeamento Encefálico , Cicloeximida/farmacologia , Emetina/farmacologia , Glutamatos/farmacologia , Ácido Glutâmico , Masculino , Memória/efeitos dos fármacos , Ouabaína/farmacologiaRESUMO
This study examined effects on memory formation produced by [Leu]enkephalin and [Met]enkephalin administration in 2 regions of the 2-day-old chick brain involved in memory formation: the intermediate medial hyperstriatum ventrale (IMHV) and the lobus parolfactorius (LPO). Basal concentrations of endogenous [Leu]enkephalin and [Met]enkephalin were determined for 5 brain regions, and effects of 1-trial peck-avoidance training on enkephalin concentrations were examined in the IMHV and LPO. [Leu]enkephalin was amnestic when administered in the IMHV but not in the LPO. In contrast, [Met]enkephalin may be amnestic when administered in the LPO but not in the IMHV. Training decreased [Met]enkephalin concentration in the LPO but not in the IMHV. Training had no effect on [Leu]enkephalin concentration in either the IMHV or the LPO. Thus, amnestic effects of [Leu]- or [Met]enkephalin administration are brain-region specific. Regional activity of endogenous [Met]enkephalin during memory formation is consistent with localized amnestic effects produced by [Met]enkephalin administration.
Assuntos
Aprendizagem da Esquiva/fisiologia , Encéfalo/fisiologia , Encefalinas/fisiologia , Rememoração Mental/fisiologia , Fatores Etários , Animais , Comportamento Apetitivo/fisiologia , Encéfalo/crescimento & desenvolvimento , Mapeamento Encefálico , Galinhas , Condicionamento Clássico/fisiologia , Encefalina Leucina/fisiologia , Encefalina Metionina/fisiologia , Masculino , Plasticidade Neuronal/fisiologia , Paladar/fisiologiaRESUMO
Chelerythrine (CHELE), a specific, potent protein kinase C (PKC) inhibitor, disrupts memory formation for a one-trial peck-avoidance task. Three predictions were made about how CHELE, injected into chick brain near the time of training, would affect memory formation, based on previous work with two classes of protein kinase inhibitors (M. R. Rosenzweig et al., 1992; P. A. Serrano et al., 1994) and the in vitro inhibition of PKC by CHELE: (a) CHELE, injected into the intermediate medial hyperstriatum ventrale, would significantly impair memory formation; (b) the amnestic dose would be approximately 10 nmol; (c) CHELE would not produce amnesia for about 45 min after training, but significantly impair memory by 60 min. Experimental tests confirmed each prediction. This study adds to evidence that PKC activity is part of a cascade of neurochemical events initiated by learning and that PKC activity shortly after training is necessary for long-term memory.
Assuntos
Comportamento Apetitivo/efeitos dos fármacos , Aprendizagem da Esquiva/efeitos dos fármacos , Encéfalo/efeitos dos fármacos , Rememoração Mental/efeitos dos fármacos , Fenantridinas/farmacologia , Proteína Quinase C/antagonistas & inibidores , Alcaloides , Animais , Comportamento Apetitivo/fisiologia , Aprendizagem da Esquiva/fisiologia , Benzofenantridinas , Encéfalo/fisiologia , Mapeamento Encefálico , Galinhas , Corpo Estriado/efeitos dos fármacos , Corpo Estriado/fisiologia , Relação Dose-Resposta a Droga , Potenciação de Longa Duração/efeitos dos fármacos , Potenciação de Longa Duração/fisiologia , Masculino , Rememoração Mental/fisiologia , Proteína Quinase C/fisiologiaRESUMO
Several experiments were conducted to examine the effects of intracranial injection of opioid peptides and antagonists on learning and memory in the chick. Pretraining injection of [leu5]enkephalin and the selective delta receptor agonist [D-Pen2,L-Pen5]enkephalin (DPLPE) into the intermediate medial hyperstriatum ventrale (IMHV) produced impairment. ICI 174,864, a delta-selective antagonist, reversed the impairment produced by either [leu5]enkephalin or DPLE, results indicating that delta receptors may play a role in learning in the chick and suggesting that the impairment produced by [leu5]enkephalin is mediated through delta opioid receptors. beta-endorphin produced a naloxone-reversible impairment in performance, which suggests that this impairment is mediated by opioid receptors. Bilateral injection of beta-endorphin into the IMHV produced impairment, as did unilateral injection into the right, but not left, IMHV. Only bilateral injections into IMHV of [leu5]enkephalin were effective. These results suggest that the effects of beta-endorphin are centrally mediated whereas the effects of [leu5]enkephalin may be localized to other brain regions or are peripherally mediated. These initial results suggest that opioids are associated with learning and memory in the chick.
Assuntos
Aprendizagem por Discriminação/efeitos dos fármacos , Endorfinas/farmacologia , Memória/efeitos dos fármacos , Rememoração Mental/efeitos dos fármacos , Córtex Visual/efeitos dos fármacos , Animais , Atenção/efeitos dos fármacos , Mapeamento Encefálico , Galinhas , Dominância Cerebral/efeitos dos fármacos , Relação Dose-Resposta a Droga , D-Penicilina (2,5)-Encefalina , Encefalina Leucina/análogos & derivados , Encefalina Leucina/farmacologia , Encefalinas/farmacologia , Masculino , Naloxona/farmacologia , Receptores Opioides/efeitos dos fármacos , Receptores Opioides delta , beta-Endorfina/farmacologiaRESUMO
To examine the role of kappa opioid receptors in memory formation, 2-day-old chicks were injected intracerebrally with either the endogenous opioid peptide dynorphin(1-13), the highly kappa selective agonist U-50,488 or the kappa selective antagonist nor-binaltorphimine (nor-BNI), given one-trial peck-avoidance training, and tested 24 h later. Dynorphin(1-13) impaired memory in a dose dependent manner at 24 h test. Injection of U-50,488 caused a biphasic dose-dependent effect on memory; low doses caused a trend toward enhanced memory and high doses caused significant impairment. Conversely, injection of low doses of nor-BNI caused a trend toward memory impairment, and higher doses caused significant memory enhancement. The results indicate that memory formation for one-trial peck-avoidance training may be modulated by kappa opioid receptor activity.
Assuntos
Animais Recém-Nascidos/psicologia , Aprendizagem da Esquiva/fisiologia , Memória/fisiologia , Receptores Opioides kappa/fisiologia , (trans)-Isômero de 3,4-dicloro-N-metil-N-(2-(1-pirrolidinil)-ciclo-hexil)-benzenoacetamida , Analgésicos/farmacologia , Animais , Aprendizagem da Esquiva/efeitos dos fármacos , Galinhas , Relação Dose-Resposta a Droga , Dinorfinas/farmacologia , Masculino , Memória/efeitos dos fármacos , Naltrexona/análogos & derivados , Naltrexona/farmacologia , Fragmentos de Peptídeos/farmacologia , Pirrolidinas/farmacologiaRESUMO
The opioid peptide dynorphin(1-13) impairs memory formation in chicks (5). We examined whether this occurs for both aversively and appetitively motivated learning. Four-day-old chicks were injected with dynorphin(1-13) into the intermediate medial hyperstriatum ventrale and trained on either a peck avoidance (PA) or an appetitive discrimination (AD) task; 2-day-old chicks were trained on PA. In 2-day-old chicks, dynorphin was amnestic for PA at 0.01, 0.03, or 0.1 mM. In 4-day-old chicks, dynorphin impaired memory formation for PA at 0.1 mM, and for AD training at 0.03 mM. Thus, similar doses of dynorphin impair memory formation for both appetitive and aversive conditioning.
Assuntos
Comportamento Apetitivo/efeitos dos fármacos , Aprendizagem da Esquiva/efeitos dos fármacos , Aprendizagem por Discriminação/efeitos dos fármacos , Dinorfinas/farmacologia , Memória/efeitos dos fármacos , Fragmentos de Peptídeos/farmacologia , Animais , Animais Recém-Nascidos , Galinhas , MasculinoAssuntos
Encéfalo , Memória , RNA , Extratos de Tecidos , Animais , Comportamento Animal , Condicionamento Psicológico , Técnicas In Vitro , RatosRESUMO
Supporting Hebb's 1949 hypothesis of use-induced plasticity of the nervous system, our group found in the 1960s that training or differential experience induced neurochemical changes in cerebral cortex of the rat and regional changes in weight of cortex. Further studies revealed changes in cortical thickness, size of synaptic contacts, number of dendritic spines, and dendritic branching. Similar effects were found whether rats were assigned to differential experience at weaning (25 days of age), as young adults (105 days) or as adults (285 days). Enriched early experience improved performance on several tests of learning. Cerebral results of experience in an enriched environment are similar to results of formal training. Enriched experience and training appear to evoke the same cascade of neurochemical events in causing plastic changes in brain. Sufficiently rich experience may be necessary for full growth of species-specific brain characteristics and behavioral potential. Clayton and Krebs found in 1994 that birds that normally store food have larger hippocampi than related species that do not store. This difference develops only in birds given the opportunity to store and recover food. Research on use-induced plasticity is being applied to promote child development, successful aging, and recovery from brain damage; it is also being applied to benefit animals in laboratories, zoos and farms.
Assuntos
Comportamento Animal/fisiologia , Comportamento/fisiologia , Encéfalo/fisiologia , Plasticidade Neuronal/fisiologia , Animais , Meio Ambiente , Humanos , Aprendizagem/fisiologiaRESUMO
This paper focuses on the temporal dimension of memory formation and storage. Is the usual two-fold separation between short-term memory (STM) and long-term memory (LTM) sufficient to encompass all the phenomena of memory? The traditional view is that STM grades into LTM. Evidence for an intermediate-term memory (ITM) has been proposed by some investigators. We have used both rats and chicks to investigate the stages of memory formation. In this paper, the advantages of chicks for this type of research are briefly discussed. Using a paradigm that produces weak training, the retention function for control chicks appears to be made up of four successive components which we have interpreted as representing the memory buffer, STM, ITM, and LTM. In experiments using a variety of kinase inhibitors, we have obtained evidence that ITM and LTM depend on different classes of protein kinase activities. Agents that act on calcium/calmodulin kinase cause amnesia in the ITM range--15 to 30 min post-training. Another class of inhibitors act on one or more of the kinases PKA, PKC, or PKG and cause amnesia by 60 min post-training, so we interpret this group of inhibitors as inhibiting the formation of LTM. However, the three-stage model of memory may be over-simple. For example some agents including [Leu]enkephalin and MK801 cause amnesia 4 or more h after training.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Envelhecimento/fisiologia , Memória de Curto Prazo/fisiologia , Rememoração Mental/fisiologia , Retenção Psicológica/fisiologia , Animais , Encéfalo/fisiologia , Galinhas , Proteínas Quinases/fisiologia , Receptores de Neurotransmissores/fisiologia , Sistemas do Segundo Mensageiro/fisiologiaRESUMO
The memorial effects of glutamate, LaCl3, ouabain, or anisomycin injection around the time of active avoidance training in mice were assessed in this study. Based on the Gibbs and Ng hypothesis of memory formation in chicks (Biobehav. Rev., 1 [1977] 113-136), it was predicted that these pharmacological agents would not only induce significant amnesia but, more specifically, short duration memory should be selectively impaired by glutamate and LaCl3, intermediate duration memory should be impaired by ouabain, and anisomycin should affect only long-lasting memories. Results of the experiments described below indicate these drugs are potent inhibitors of memory formation in rodents. In addition, LaCl3-induced amnesia was fully prevented by CaCl2. However, the mechanism by which glutamate and ouabain affected memory may not be exactly as described by Gibbs and Ng: gamma-D-glutamylglycine and diphenylhydantoin did not completely prevent glutamate- and ouabain-induced amnesias, respectively. Finally, all amnestic agents induced amnesia that developed within minutes of training, and the time course of development of amnesia for each drug could not be distinguished from one another. These data are discussed in terms of their implications for the Gibbs and Ng model of memory formation.
Assuntos
Aprendizagem da Esquiva/efeitos dos fármacos , Memória , Animais , Anisomicina/farmacologia , Cloreto de Cálcio/farmacologia , Dipeptídeos/farmacologia , Glutamatos/farmacologia , Ácido Glutâmico , Hipocampo/efeitos dos fármacos , Hipocampo/fisiologia , Lantânio/farmacologia , Masculino , Memória/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos , Ouabaína/farmacologia , Fenitoína/farmacologiaRESUMO
Several experiments were conducted to test whether, as suggested by Welch et al. in this journal, mere group living (social stimulation) can account for the significant differences in measures of brain anatomy and brain chemistry that develop between rodents housed in groups in enriched environments and rodents housed singly in restricted environments; the alternative hypothesis was that features of the inanimate environment can significantly affect brain measures of animals living in a social group. Groups of 12 male rats were assigned for 30 days to several types of environment: (a) large cage without stimulus objects, (b) large cage containing varied stimulus objects, (c) large cage containing a maze whose pattern of barriers was changed daily, and (d) a seminatural outdoor environment; in each experiment, littermates of rats in the social conditions were housed in isolation in small colony cages. At the end of the 30-day period, measures were taken of weights of brain regions, RNA and DNA contents of regions of cerebral cortex, and acetylcholinesterase activities of brain regions. Although the number of rats housed together was constant for conditions a--d and cage size was constant for conditions a--c, the magnitudes of the cerebral measures varied significantly as a function of the inanimate stimulus conditions. The differences from isola;ion-housed littermates was greatest in condition d and smallest in condition a. Thus, social grouping alone is inadequate to explain the cerebral effects of enriched environments and the inanimate stimulus conditions must be taken into account.
Assuntos
Córtex Cerebral/enzimologia , Meio Social , Acetilcolinesterase/metabolismo , Animais , Encéfalo/anatomia & histologia , Colinesterases/metabolismo , DNA/metabolismo , Masculino , Tamanho do Órgão , RNA/metabolismo , Ratos , Privação SensorialRESUMO
Many studies suggest that protein synthesis is required for formation of long-term memory. To test whether the protein synthesis inhibitor anisomycin (ANI) actually inhibits long-term memory formation or whether apparent amnesia could be attributed to state-dependency, chicks were both trained and tested under the influence of anisomycin (ANI). Two-day-old cockerels were trained in a 1-trial passive avoidance task. Intracerebral injections (10 microliters per hemisphere) of either saline (SAL) or 11.0 mM ANI were made into the medial hyperstriatum ventrale 5 min pretraining and 5 min pretest. The ANI inhibited cerebral protein synthesis by 70-80%, a level necessary to cause amnesia. Chicks that pecked a small bead dipped in methylanthranilate (MeA) and were injected with SAL both pretraining and pretest avoided pecking at test, showing memory for the bitter substance; chicks given ANI pretraining and SAL pretest pecked at the bead during test, which suggests amnesia. However, those given ANI both pretraining and pretest showed marked avoidance at test. Chicks trained to peck at a small bead dipped in water and given injections of either SAL or ANI pretraining and SAL pretest pecked readily at test. However, water-trained chicks given ANI pretest, regardless of pretraining injection, showed significantly higher avoidance at test. We conclude that peck aversion in the ANI-MeA-ANI group was not due to state-dependency but to generalized avoidance induced by pretest ANI.
Assuntos
Amnésia/induzido quimicamente , Anisomicina/farmacologia , Aprendizagem da Esquiva/efeitos dos fármacos , Encéfalo/fisiopatologia , Pirrolidinas/farmacologia , Amnésia/metabolismo , Amnésia/fisiopatologia , Animais , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Galinhas , Proteínas do Tecido Nervoso/metabolismoRESUMO
Anisomycin, an inhibitor of brain protein synthesis, was used to control the time and duration of protein synthesis occurring in mice after they were trained on a one-trial passive avoidance task. It was found that if synthesis was strongly inhibited for 6 to 8 hr, a high percentage of the animals was amnesic. However, if small amounts of protein synthesis were allowed to occur by permitting intervals of partial recovery of protein synthesis during the period of inhibition, then fewer mice were amnesic. The longer the duration of this interval of controlled synthesis and the closer it occurred to training, the greater the percentage of subjects remembering the training.
Assuntos
Encéfalo/metabolismo , Memória , Proteínas do Tecido Nervoso/biossíntese , Amnésia/induzido quimicamente , Animais , Anisomicina/administração & dosagem , Anisomicina/farmacologia , Aprendizagem da Esquiva/efeitos dos fármacos , Química Encefálica/efeitos dos fármacos , Depressão Química , Feminino , Humanos , Injeções , Memória/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos C57BL , Proteínas do Tecido Nervoso/antagonistas & inibidores , Tempo de Reação/efeitos dos fármacos , Fatores de TempoRESUMO
Inhibition of brain protein synthesis by anisomycin and acetoxycycloheximide was studied in mice for its biochemical and behavioral effects. By employing both drugs in low doses in a series of injections, we were able to maintain inhibition of protein synthesis of 80% or greater for up to 14 hr without causing detectable permanent physiological impairment. The drugs were employed as amnestic agents in mice trained to avoid footshock in a T-maze. As the duration of inhibition increased, the percentage of mice classed as amnesic increased. This amnesia could be reduced by increasing (a) the rate of acquisition, or (b) the number of training trials. Anisomycin was shown to cause a significant degree of amnesia for the escape component as well as the avoidance component of the learning. A single injection of anisomycin given 15 min prior to training did not cause significant changes in the acquisition or retention of avoidance conditioning, when comparison was made with saline-injected controls. Only additional injections given after training to prolong inhibition caused amnesia. Thus, those injections critical in obtaining amnesia were given at a time at which interference with acquisition could not have occurred, so the results bear clearly on memory processes.