Effect of topical medication on the nasomaxillary skin-fold microbiome in French bulldogs.

BACKGROUND: Host-microbe interactions may influence dermatitis pathogenesis in the nasomaxillary folds of French bulldogs, which is often complicated by secondary bacterial and fungal infections. OBJECTIVE: To assess the skin-fold microbiome in systemically healthy French bulldogs and to determine the influence of topical medications on this microbiome. ANIMALS: Nineteen healthy French bulldogs. METHODS AND MATERIALS: Next-generation DNA sequencing was applied to characterise the microbiome composition in the nasomaxillary folds of systemically healthy French bulldogs. Subsequently, the effect of two topical products on the fold microbiome was assessed. Seven dogs were treated with a protease product (Kalzyme; enzyme) that inhibits biofilm formation without biocidal activity, six dogs were treated with a 2% chlorhexidine diacetate solution (Nolvasan; CHX) with biocidal activity, and six dogs were untreated. Dogs were randomly assigned to each group, and the investigator was blinded. RESULTS: The primary skin bacterial phyla inhabiting the folds at inclusion were Firmicutes, Actinobacteria and Proteobacteria. The primary skin fungal phyla were Ascomycota and Basidiomycota. Topical treatment increased the diversity of bacterial and fungal compositions over time (increase in microbial diversity score: enzyme 38%, chlorhexidine 11%, control <5%) and the relative abundance of pathogens reduced significantly (enzyme, P = 0.028; CHX, P = 0.048). A clear correlation (r2 = 0.83) was observed between the abundance of clinically relevant pathogens and microbial diversity. CONCLUSIONS: The nasomaxillary skin-fold microbiome of healthy French bulldogs contained a high abundance of clinically relevant pathogens (mean 36.4%). Topical therapy with enzyme increased microbial diversity of skin folds and reduced the relative abundance of pathogens.

Microchip Health Monitoring System Using the FLL Circuit.

Here a solution for a Microchip Health Monitoring (MHM) system using MTOL (Multi-Temperature Operational Life) reliability testing assessment data is proposed. The module monitors frequency degradation over time compared to lab tested data. Since trends in performance degradation in recently developed devices have transitioned from multiple failure mechanisms to a single dominant failure mechanism, development of the monitor is greatly simplified. The monitor uses a novel circuit customized to deliver optimum accuracy by combining the concepts of ring oscillator (RO) and phase locked loop (PLL) circuits. The modified circuit proposed is a new form of the frequency locked loop (FLL) circuit. We demonstrate that the collection of frequency degradation data from the ring circuits of each test produces Weibull distributions with steep slopes. This implies that the monitor can predict accurate end-of-life (EOL) predictions at early stages of chip degradations. The design of the microchip health monitoring system projected in this work can have great benefit in all systems using FPGA and ASIC devices.

Hair and saliva analysis fails to accurately identify atopic dogs or differentiate real and fake samples.

BACKGROUND: The availability of direct-to-consumer medical testing for human and veterinary health conditions has increased in recent years. For allergies, several companies market proprietary hair and saliva tests directly to pet owners. These tests have not been validated and there is limited regulatory oversight for such tests in veterinary medicine. HYPOTHESIS/OBJECTIVES: To examine the accuracy and reproducibility of a commercial direct-to-consumer hair and saliva allergen test. ANIMALS: Seven healthy animals (six dogs, one cat); six animals (five dogs, one cat) with atopic dermatitis; 11 samples of synthetic fur and sterile saline. METHODS AND MATERIALS: Duplicate animal hair and saliva, and 11 synthetic fur and saline samples were collected (total samples 35) and submitted to the company for analysis, yielding 12,075 outcomes for statistical analysis. RESULTS: Positive test results were provided by the direct-to-consumer pet allergy for all submitted samples, including synthetic fur and saline. The test results for healthy and atopic animal samples were no different from each other or from synthetic fur and saline samples. Reproducibility for paired samples was not different from random chance. The results for real animals correlated strongly with results for synthetic fur and saline samples (r = 0.71, P < 0.05). CONCLUSIONS AND CLINICAL IMPORTANCE: The direct-to-consumer hair and saliva test for pet allergies examined in this study performed no better than chance and the results were not reproducible.

Pediatric airway study: Endoscopic grading system for quantifying tonsillar size in comparison to standard adenotonsillar grading systems.

SIGNIFICANCE: Current grading systems may not allow clinicians to reliably document and communicate adenotonsillar size in the clinical setting. A validated endoscopic grading system may be useful for reporting tonsillar size in future clinical outcome studies. This is especially important as tonsillar enlargement is the cause of a substantial health care burden on children. OBJECTIVE: To propose and validate an easy-to-use flexible fiberoptic endoscopic grading system that provides physicians with a more accurate sense of the three-dimensional relationship of the tonsillar fossa to the upper-airway. METHODS: 50 consecutive pediatric patients were prospectively recruited between February 2015 and February 2016 at a pediatric otolaryngology outpatient clinic. The patients had no major craniofacial abnormalities and were aged 1 to 16years. Each patient had data regarding BMI, Friedman palate position, OSA-18 survey results collected. For each child, digital video clips of fiberoptic nasopharyngeal, oropharyngeal and laryngeal exams were presented to 2 examiners. Examiners were asked to independently use the proposed Endoscopic tonsillar grading system, the Brodsky tonsillar grading scale, the Modified Brodsky tonsillar grading scale with a tongue depressor, and the Parikh adenoid grading system to rate adenotonsillar hypertrophy. Cohen's Kappa and weighted Kappa scores were used to assess interrater reliability for each of the four grading scales. The Spearman correlation was used to test the associations between each scale and OSA-18 scores, as well as Body Mass Index (BMI). RESULTS: 50 pediatric patients were included in this study (mean age 6.1years, range of 1year to 16years). The average BMI was 20. The average OSA-18 score was 61.7. The average Friedman palate position score was 1.34. Twelve percent of the patients had a Friedman palate position score≥3, which made traditional Brodsky grading of their tonsils impossible without a tongue depressor. All four scales showed strong agreement between the two raters. The weighted Kappa was 0.83 for the Modified Brodsky scale, 0.89 for the Brodsky scale, 0.94 for the Parikh scale to 0.98 for the Endoscopic scale (almost perfect agreement). The Endoscopic scale showed the most consistent agreement between the raters during the study. There was a moderate association between the Parikh adenoid grading system with OSA-18 scores (Spearman's ρ=0.58, p<0.001) compared to a low association of the tonsillar grading systems with OSA- 18 scores. None of the scales correlated with patient BMI. CONCLUSIONS: The proposed Endoscopic tonsillar grading system is as reliable of a method of grading tonsillar size as conventional grading systems. It offers the advantage of allowing for critical evaluation of the tonsils without any anatomic distortion which may occur with the use of a tongue blade. This new validated endoscopic grading system provides a tool for communicating the degree of airway obstruction at the level of the oropharynx regardless of Friedman palate position and may be used in future outcomes projects.