Antineuroinflammatory Effect of Amburana cearensis and Its Molecules Coumarin and Amburoside A by Inhibiting the MAPK Signaling Pathway in LPS-Activated BV-2 Microglial Cells.

Microglia plays an important role in the neuroinflammatory response, identified as one of the major factors in the development and progression of neurodegenerative diseases. Amburana cearensis and its bioactive compounds, including coumarin (CM), vanillic acid (VA), and amburoside A (AMB), exert antioxidant, anti-inflammatory, and neuroprotective activities, on 6-OHDA-induced neurotoxicity in rat mesencephalic cells determined by our group. The present study investigated the anti-inflammatory effect of the dry extract from A. cearensis (DEAC), CM, AMB, and VA on lipopolysaccharide- (LPS-) stimulated microglial cells and elucidated the possible molecular mechanism of action. The DEAC was characterized by HPLC-PDA (chemical markers: CM, AMB, and VA). The BV-2 microglial cell line was pretreated with increasing concentrations of DEAC, CM, AMB, or VA in the presence or absence of LPS to evaluate the toxicity and anti-inflammatory activity. The cytotoxicity of DEAC, CM, AMB, or VA on BV-2 cells was evaluated by the MTT test, the free radical scavenging activity of test drugs was investigated, and the nitric oxide (NO) production was determined using the Griess reagent, while cytokine levels were measured by ELISA. The expressions of toll-like receptor 4 (TLR-4), nuclear factor kappa B (NF-κB), MAPK members (JNK and ERK1/2), and iNOS were determined through Western blot analysis. DEAC, CM, AMB, or VA (5-100 µg/mL) did not induce any detectable cytotoxicity in BV-2 cells. All test drugs (100 µg/mL) showed free radical scavenging activity (hydroxyl and superoxide radicals); however, only DEAC, CM, and AMB (5-100 µg/mL) significantly reduced NO production. DEAC (100 µg/mL), as well as CM (50 and 100 µg/mL) and AMB (25 µg/mL), reduced at least 50% of NO produced and markedly decrease the production of TNF-α and IL-6 but they did not significantly affect IL-10 levels. Only DEAC (100 µg/mL) and AMB (25 µg/mL) reduced the expression of iNOS, and they did not affect arginase activity. DEAC (100 µg/mL) suppressed the activation of the MAPKs JNK and ERK1/2 in LPS-activated BV-2 cells but it did not suppress the expression of TLR-4 nor the phosphorylation of NF-κB. In conclusion, DEAC, CM, and AMB exerted anti-inflammatory activity in LPS-activated microglial cells as observed by the reduction in the production of inflammatory mediators and the expression of iNOS. We identified the MAPK signaling pathway as a probable mechanism of action to the anti-inflammatory effects observed.

Efeito do tamanho da semente, subtrato ambiente na produção de mudas de Copernicia Hospita Martius / Effect of seed size, substrate, and environment on the production of Copernicia hospita Martius seedlings

A carnaúba hospedeira faz parte de um grupo de plantas da família Arecaceae, de relevante importância social e econômica para as regiões tropicais. Neste trabalho, objetivou-se determinar a influência do tamanho da semente, tipo de substrato e do ambiente na produção de mudas de carnaúba hospedeira (Copernicia hospita Martius). As sementes de diferentes tamanhos (pequena, média, grande e mistura) foram semeadas em substratos constituídos de areia vermelha + bagana de carnaúba + húmus (2,5:2,5:1 em volume) e solo + arisco + composto orgânico Polefértil (2:2:1 em volume) e cultivadas em ambientes distintos (pleno sol e casa de vegetação). As variáveis analisadas foram: comprimento do limbo foliar principal, área foliar, diâmetro do coleto, comprimento da maior raiz e massa da matéria seca da parte aérea e radicular. O tamanho da semente exerceu efeito significativo sobre todas as variáveis. O substrato influenciou, significativamente, o comprimento do limbo e da maior raiz e área foliar. Quanto ao ambiente, as únicas variáveis não afetadas por esse fator foram as produções de matéria seca, tanto da parte aérea como radicular. Para a produção de mudas de C. hospita recomenda-se a utilização de sementes grandes, semeadas no substrato constituído de areia vermelha + bagana de carnaúba + húmus, em cultivo a pleno sol.

The 'Hostess' Carnauba belongs to a group of plants from the Arecaceae family, which is of relevant social and economic importance for tropical areas in Brazil. The objective of this research was to study the effect of the size of the seed , substrate, and environment on the production of seedlings of 'Hostess' Carnauba (Copernicia hospita Martius). Seeds of different sizes (small, medium, large, and a mixture of different sizes) were planted in different substrates composed of red sand + carnauba straw + humus (2,5:2,5:1 in volume) and soil + dark sand + organic compost Polefértil (2:2:1 in volume), and cultivated in different environments (outdoors and indoors). The analyzed variables were: leaf length , leaf area, stem diameter, length of the longest root, and dry matter of the above and below ground parts. The size of the seed greatly affected all of the studied variables. Substrate type significantly affected leaf length, length of the longest root, and leaf area. The only variables not affected by the environment were of the dry matter from above and below ground parts. Thus, the results suggest that large seeds should be recommended for the production of C. hospita seedlings, and should be planted in a subtract composed of red sand + carnauba straw + humus for unshaded outdoor cultivation.

Biodiversidade em sistemas agroecológicos no município de Choró, CE, Brasil / Biodiversity in agroecological systems in Choró-CE, Brazil

Tendo em vista que a biodiversidade é de fundamental importância para a qualidade do agrossistema, este trabalho teve o objetivo de avaliar componentes da biodiversidade em sistemas agroecológicos conduzidos por agricultores familiares no município de Choró, Ceará (CE). Para tanto, áreas cultivadas foram selecionadas pelo tempo de adoção das técnicas de cultivo em consórcio, bem como áreas de vegetação natural próximas, utilizadas como referência. Nessas áreas foram avaliados a fauna edáfica, a flora do extrato herbáceo e arbóreo, o solo e os coprólitos, amostrados aleatoriamente ou em transectos. A riqueza de grupos e os índices de diversidade de Shannon e equalibilidade de Pielou também foram calculados. Os resultados obtidos permitem concluir que o consórcio favoreceu a diversidade da fauna do solo e da flora comparativamente às áreas de vegetação natural.

Biodiversity is important for the quality of agroecosystems. This study aims to analyze some components of biodiversity in agroecological systems lead by family farmers in the municipality of Choró, CE. Agricultural areas were selected by the time of adoption of intercropping systems as well as areas under natural vegetation used as reference. The soil fauna, the flora of the herbaceous and tree-shrub extracts, soil samples and coprolites were collected randomly or in transects to be analysed. The richness, and diversity and uniformity index (Shannon and Pielou, respectively) were also calculated. The adopted intercropping systems favour the soil fauna and flora diversity when compared to the natural vegetation areas.