Ketoacidosis - Where Do the Protons Come From?

In extreme conditions ketosis can progress to ketoacidosis, a dangerous and potentially life-threatening condition. Ketoacidosis is most common in new or poorly treated type 1 diabetes. The acidosis is usually attributed to the 'acidic' nature of the ketone bodies (acetoacetate, 3-hydroxybutyrate, and acetone). However, acetoacetate and 3-hydroxybutyrate are produced not as acids but as their conjugate bases, and acetone is neither an acid nor a base. This raises the question of why severe ketosis is accompanied by acidosis. Here, we analyze steps in ketogenesis and identify four potential sources: adipocyte lipolysis, hydrolysis of inorganic pyrophosphate generated during synthesis of fatty acyl-coenzyme A (CoA), the reaction catalyzed by an enzyme in the ß-oxidation pathway (3-hydroxyacyl-CoA dehydrogenase), and increased synthesis of CoA.

Historical analysis of oil and gas well plugging in New York: is the regulatory system working?

The aim of this work was to evaluate New York State's regulatory program for plugging inactive oil and gas wells. Analysis of reports from the Division of Mineral Resources, Department of Environmental Conservation, reveals that three-fourths of the state's abandoned oil and gas wells were never plugged. Inadequate enforcement efforts have resulted in steady increases of unplugged oil and gas wells abandoned since 1992. Further, no program exists or is proposed to monitor abandoned wells which were plugged. These results strongly suggest that comprehensive reform and increased agency resources would be required to effectively regulate conventional oil and gas development in New York. Industrial expansion into shale oil and gas development should be postponed to avoid adding stress to an already compromised regulatory system.