Assessing inbred-hybrid relationships for developing drought-tolerant provitamin A-quality protein maize hybrids.

Drought-tolerant early-maturing maize (Zea mays L.) inbred lines with high levels of provitamin A (PVA) and quality protein (QPM) are urgently needed for development of superior hybrids to mitigate malnutrition and to intensify maize production and productivity in sub-Saharan Africa (SSA). This study was designed to identify early-maturing inbred lines with combined tolerance to drought, elevated tryptophan, and PVA contents; to examine inbred-hybrid relationships for tryptophan and PVA accumulation; and to select hybrids with outstanding grain yield (GY) performance. A total of 64 inbred lines and six checks, plus 96 hybrids and four checks, were evaluated under drought and well-watered environments in Nigeria for 2 yr. Eighteen parental lines and 54 derived hybrids were assayed for tryptophan and PVA contents. Ten drought-tolerant inbred lines with high tryptophan and elevated PVA levels were identified in the top 10 hybrid combinations across managed drought and well-watered conditions. The inbred-hybrid relationship was significant for GY under each and across the two contrasting environments. Significant average heterosis was found for tryptophan and PVA under well-watered conditions. This indicated that the selected inbred lines could be used for developing high-yielding PVA-QPM hybrids tolerant to drought stress in SSA. The 10 top-performing PVA-QPM hybrids identified are being extensively evaluated in different locations and subsequently in on-farm trials for commercialization throughout SSA.

Genetic characterization of cassava (Manihot esculenta Crantz) genotypes using agro-morphological and single nucleotide polymorphism markers.

Dearth of information on extent of genetic variability in cassava limits the genetic improvement of cassava genotypes in Sierra Leone. The aim of this study was to assess the genetic diversity and relationships within 102 cassava genotypes using agro-morphological and single nucleotide polymorphism markers. Morphological classification based on qualitative traits categorized the germplasm into five different groups, whereas the quantitative trait set had four groups. The SNP markers classified the germplasm into three main cluster groups. A total of seven principal components (PCs) in the qualitative and four PCs in the quantitative trait sets accounted for 79.03% and 72.30% of the total genetic variation, respectively. Significant and positive correlations were observed between average yield per plant and harvest index (r = 0.76***), number of storage roots per plant and harvest index (r = 0.33*), height at first branching and harvest index (0.26*), number of storage roots per plant and average yield per plant (r = 0.58*), height at first branching and average yield per plant (r = 0.24*), length of leaf lobe and petiole length (r = 0.38*), number of leaf lobe and petiole length (r = 0.31*), width of leaf lobe and length of leaf lobe (r = 0.36*), number of leaf lobe and length of leaf lobe (r = 0.43*), starch content and dry matter content (r = 0.99***), number of leaf lobe and root dry matter (r = 0.30*), number of leaf lobe and starch content (r = 0.28*), and height at first branching and plant height (r = 0.45**). Findings are useful for conservation, management, short term recommendation for release and genetic improvement of the crop.

Phenotypic characterization and validation of provitamin A functional genes in early maturing provitamin A-quality protein maize (Zea mays) inbred lines.

The number of drought and low-N tolerant hybrids with elevated levels of provitamin A (PVA) in sub-Saharan Africa could increase when PVA genes are optimized and validated for developed drought and low-N tolerant inbred lines. This study aimed to (a) determine the levels of drought and low-N tolerance, and PVA concentrations in early maturing PVA-quality protein maize (QPM) inbred lines, and (b) identify lines harbouring the crtRB1 and LcyE genes as sources of favourable alleles of PVA. Seventy early maturing PVA-QPM inbreds were evaluated under drought, low-N and optimal environments in Nigeria for two years. The inbreds were assayed for PVA levels and the presence of PVA genes using allele-specific PCR markers. Moderate range of PVA contents was observed for the inbreds. Nonetheless, TZEIORQ 55 combined high PVA concentration with drought and low-N tolerance. The crtRB1-3'TE primer and the KASP SNP (snpZM0015) consistently identified nine inbreds including TZEIORQ 55 harbouring the favourable alleles of the crtRB1 gene. These inbreds could serve as donor parents of the favourable crtRB1-3'TE allele for PVA breeding in maize.