Adipose-Derived Stem Cells Decrease Bone Morphogenetic Protein Type 2-Induced Inflammation In Vivo.

PURPOSE: Recombinant human bone morphogenetic protein type 2 (rhBMP-2) has been used to promote bone regeneration. In contrast, some reports have suggested rhBMP-2 does not provide advantages over autogenous bone grafting owing to the undesirable postoperative symptoms of this growth factor. Because the undesirable symptoms of rhBMP-2 are usually promoted by inflammation, this study evaluated the in vivo effect of human adipose-derived stem cells (ASCs) incorporated into polylactic co-glycolic acid (PLGA) scaffolds in decreasing the inflammatory response induced by a low dose of rhBMP-2. MATERIALS AND METHODS: PLGA scaffolds were characterized and loaded with rhBMP-2 1, 2.5, or 5 µg per scaffold (n = 6) and the in vitro released protein amounts were quantified at 7 hours and 1, 7, and 21 days after loading (n = 3). The muscle tissue of 6 beagles received the following treatments: PLGA, PLGA plus rhBMP-2 (2.5 µg), and PLGA plus rhBMP-2 plus ASCs (1 × 10(6) ASCs). The samples were evaluated 45 days after surgery. Statistical analyses were performed and the P value was set at .05. RESULTS: PLGA plus rhBMP-2 plus ASCs yielded the smallest number of inflammatory foci (P < .001) and giant cells (P < .001) and the largest number of angiogenesis sites (P < .001). CONCLUSIONS: Human ASCs administered in vivo into PLGA scaffolds with a low dose of rhBMP-2 decrease tissue inflammation and increase angiogenesis in muscular sites.

Adipose-derived stem cells incorporated into platelet-rich plasma improved bone regeneration and maturation in vivo.

BACKGROUND/AIM: Some cases of tooth loss related to dental trauma require bone-grafting procedures to improve the aesthetics before prosthetic rehabilitation or to enable the installation of dental implants. Bone regeneration is often a challenge and could be largely improved by mesenchymal stem cells therapy. However, the appropriate scaffold for these cells still a problem. This study evaluated the in vivo effect of human adipose-derived stem cells incorporated into autogenous platelet-rich plasma in bone regeneration and maturation. MATERIAL AND METHODS: Adipose-derived stem cells were isolated from lipoaspirate tissues and used at passage 4. Immunophenotyping and multilineage differentiation of cells were performed and mesenchymal stem cells characteristics confirmed. Bicortical bone defects (10 mm diameter) were created in the tibia of six beagle dogs to evaluate the effect of adipose-derived stem cells incorporated into platelet-rich plasma scaffolds, platelet-rich plasma alone, autogenous bone grafts, and clot. Samples were removed 6 weeks postsurgeries and analyzed by quantification of primary and secondary bone formation and granulation tissue. RESULTS: Adipose-derived stem cells incorporated into platelet-rich plasma scaffolds promoted the highest bone formation (primary + secondary bone) (P < 0.001), the highest bone maturation (secondary bone) (P < 0.001), and the lowest amount of granulation tissue (P < 0.001). CONCLUSIONS: Adipose-derived stem cells incorporated into platelet-rich plasma scaffolds promote more bone formation and maturation, and less granulation tissue in bone defects created in canine tibia. Therefore, platelet-rich plasma can be considered as a candidate scaffold for adipose-derived stem cells to promote bone regeneration.

The role of parathyroid hormone-related protein in the regulation of osteoclastogenesis by cementoblasts.

BACKGROUND: Parathyroid hormone-related protein (PTHrP) promotes osteoclastogenesis by inhibiting expression of osteoprotegerin (OPG), a decoy receptor for the receptor activator of nuclear factor kappa B (RANK), and by enhancing production of RANK ligand (RANKL) by osteoblasts. However, little is known regarding the role of PTHrP in regulating cementoblast-mediated osteoclastogenesis. METHODS: This study determined the impact of PTHrP on osteoclastogenesis using: 1) OCCM-30 (immortalized murine cementoblasts), 2) RAW 264.7 cells (murine myeloid cells), or 3) OCCM-30 plus RAW 264.7 cells. Cells were treated with PTHrP (1-34), RANKL, or PTHrP and RANKL combined. Enzyme-linked immunosorbent assays (ELISAs) for OPG and RANKL were performed on media and cell lysates, and tartrate-resistant acid phosphatase (TRAP) and mRNA detection for the osteoclast associated receptor (OSCAR) were performed. RESULTS: The highest numbers of TRAP-positive cells and cells expressing OSCAR were found in the RAW cell group treated with either RANKL alone or RANKL and PTHrP. TRAP-positive cells were fewer when OCCM cells were co-cultured with RAW, but the greatest numbers were still with both PTHrP and RANKL. OPG levels were highest from OCCM cells and PTHrP decreased these levels. In contrast, RANKL levels were low in OCCM cell lysates and PTHrP increased RANKL. In vivo studies also revealed high osteoclastic activity surrounding developing teeth in mice administered PTH. CONCLUSIONS: These results demonstrate that PTHrP influences the balance of OPG and RANKL production by cementoblasts, and further indicate that this effect, in the context of surrounding cells, might have a significant impact on osteoclastogenesis, root resorption, and tooth eruption.

Leucine-rich amelogenin peptide: a candidate signaling molecule during cementogenesis.

BACKGROUND: Cementum is a critical mineralized tissue; however, control of its formation remains undefined. One hypothesis is that enamel matrix proteins/peptides secreted by ameloblasts and/or epithelial rest cells contribute to the control of cementum formation via epithelial-mesenchymal interactions. Here, we focused on determining whether or not leucine-rich amelogenin peptide (LRAP), translated from an alternatively spliced amelogenin RNA, altered cementoblast behavior. METHODS: Immortalized murine cementoblasts (OCCM-30) were exposed to LRAP and evaluated for: 1) proliferative activity; 2) gene expression using Northern blot for Cbfal (core binding factor alpha-1); OCN (osteocalcin), OPN (osteopontin), and real-time reverse transcription-polymerase chain reaction (RT-PCR) for OPG (osteoprotegerin); and RANKL (receptor activator of NF-kappaB ligand); 3) signaling pathway using inhibitors of PKA (THFA), PKC (GF109203X), and MAPK (UO126); and 4) mineralization evaluated by von Kossa and Alizarin-red. RESULTS: LRAP had no effect on cell proliferation up to 6 days, with a decrease in cell growth observed at the highest dose by 9 days versus untreated cells. LRAP down regulated OCN and up regulated OPN in a dose- and time-response fashion, and inhibited the capacity of mineral nodule formation. Transcripts for OPG were increased in LRAP-treated cells compared to control, but RANKL mRNA levels were not affected. Core binding factor alpha (Cbfa) mRNA, expressed constitutively, was not affected by LRAP. Signaling pathway assays suggested involvement of the MAPK pathway, since the addition of the MAPK inhibitor suppressed OPN expression in LRAP-treated cells. CONCLUSION: Leucine-rich amelogenin peptide appears to have a direct effect on cementoblast activity that may prove significant during development as well as in regeneration of periodontal tissues.

The use of bovine screws to promote bone formation using a tibia model in dogs.

The objective of this study was to evaluate the use of a unique resorbable bovine bone screw to stimulate bone formation. Bovine bone screws were inserted in the tibia of beagle dogs. Each animal received 8 screws, divided into groups A (screws + no membranes), B (screws + titanium reinforced membranes), and C (bone defects treated with autogenous bone grafts). Animals were killed at 2, 4, and 6 months. New bone was measured with a periodontal probe and reported an average of 7.4 mm in vertical bone gain for group B, 3.6 mm for group A, and 1.7 mm for group C. Submission to Kruskal-Wallis test showed statistical differences among groups (P < .05). Histologic examination revealed an intimate contact between the newly formed bone and the resorbing bone screws. We conclude that bovine bone screws provide an environment for new bone formation and thus may provide an alternative therapy for enhancing bone formation vertically, including for regenerative procedures as well as before implant therapy.

Regeneração tecidual guiada em recessões gengivais - o uso do enxerto gengival autógeno e das membranas de colágeno - uma revisão bibliográfica / Guided tissue regeneration in gingival recessions ­ the use of autogenous gingival enxeration and collagen membranes ­ a bibliographic review

Na odontologia atual, as recessões dos tecidos gengivais são um dos principais motivos de queixa dos pacientes nos consultórios e clínicas odontológicas. O processo de regeneração pode ser definido como a substituição dos tecidos perdidos pelos mesmos tipos teciduais originalmente existentes, recuperando totalmente a arquitetura e a função da área. As técnicas de Regeneração Tecidual Guiada (RTG) são regularmente apresentadas na literatura utilizando diversos materiais para reconstituir o tecido gengival perdido, como o enxerto gengival autógeno e as membranas de colágeno. A etiologia das recessões gengivais é de caráter multifatorial onde os fatores podem agir sozinhos ou concomitantemente. Dentre os fatores etiológicos podemos citar: a posição dos dentes na arcada, sequelas das doenças periodontais, sequelas da movimentação ortodôntica e trauma de escovação. O fato é que, a recessão dos tecidos gengivais é irreversível e pode causar defeitos e sequelas indesejáveis nos pacientes tais como, estética alterada do sorriso (dentes grandes), sensibilidade dentinária (dor) e predisposição ao desenvolvimento de doença periodontal (perda dental), pois compromete a qualidade e quantidade de gengiva ceratinizada que faz parte do periodonto de proteção. Segundo a Academia Americana de Periodontia (AAP), a dentina radicular nunca deve ficar exposta e pode ser recoberta de duas formas viáveis: 1) através de restauração com material não biológico (resina composta ou cimento ionômero de vidro); e 2) cirurgicamente com material biológico através de enxerto gengival autógeno ou membranas de colágeno. Por essa razão o objetivo do estudo foi elaborar uma revisão bibliográfica sobre a regeneração tecidual guiada visando à recuperação das recessões gengivais.(AU)

In current dentistry, recessions of gingival tissues are a major reason for patients' complaints in dental offices and clinics. The regeneration process can be defined as the replacement of tissues lost by the same tissue types originally existing, fully recovering the architecture and function of the area. Guided Tissue Regeneration (RTG) techniques are regularly presented in the literature using various materials to reconstitute lost gingival tissue, such as autogenous gingival graft and collagen membranes. The etiology of gingival recessions is multifactorial where factors can act alone or concomitantly. Among the aetiological factors we can mention: the position of the teeth in the arch, sequelae of periodontal diseases, sequelae of orthodontic movement, and brushing trauma. The fact is that the recession of the gingival tissues is irreversible and can cause undesirable defects and sequelae in patients such as altered aesthetics of the smile (large teeth), dentin sensitivity (pain) and predisposition to the development of periodontal disease (dental loss), because it compromises the quality and quantity of keratinized gingiva that is part of the periodontal protection. According to the American Academy of Periodontology (AAP), root dentin should never be exposed and can be covered in two viable ways: 1) through restoration with non-biological material (composite resin or glass ionomer cement) and 2) surgically with material through autologous gingival graft or collagen membranes. For this reason the objective of this study was to elaborate a literature review on guided tissue regeneration aiming at the recovery of gingival recessions.(AU)

Development of tooth germ heterotopically grafted within the ear skin. An histological study in the rat.

The main goal of this study was the analysis of the developmental potentiality of tooth germ from late bell stage on, after its heterotopic placement within the skin. Teeth germs of newborn rats were grafted within a skin pouch of the ear of adult rats. Seven to fourteen days after grafting, dental germs developed normal dental structures in which ameloblasts and odontoblasts were well differentiated. Twenty to forty-one days after graft, the inflammatory host reaction destroyed the dental developed tissues by cell infiltration. The dentin of the grafts was of osteoid characteristics, and its size increased depending on grafting time until the complete substitution of all dental tissues. This atypical dentin showed several degrees of polymerisation from collagen fibres smooth dentin devoid near the graft a to fibres rich dentin far from the dental germ. Present results suggest that this type of dental graft could be a valuable model to study the self-development of dental tissues and the reactive mechanisms taking place after dental injuries.

Evolución del germen dental trasplantado heterotópicamente en la dermis. Un estudio histológico en la rata / Development of tooth germ heterotopically grafted within the ear skin. An histological study in the rat

En este estudio hemos analizado la potencialidad de desarrollo del germen dental en fase de corona. Para ello, los gérmenes dentales de rata recién nacida fueron trasplantados en las bolsas dérmicas de la oreja de ratas isogénicas. Siete y catorce días después del trasplante, se desarrollaron las estructuras morfológicas dentales típicas, con ameloblastos y odontoblastos bien diferenciados. Ulteriormente, los procesos de reacción inflamatoria del tejido huésped con infiltración celular abocaron a la desaparición de los tejidos dentales. Desde el primer momento de nuestro análisis, los trasplantes desarrollaron una dentina anómala u osteoide cuyo tamaño fue paulatinamente incrementando con el tiempo, llegando a sustituir al resto de los tejidos dentales. Esta dentina osteoide, fruto tanto de la transformación de los odontoblastos del trasplante en células de alta actividad secretora como de la inducción que el germen dental ejerce en el tejido huésped circundante; y a diferencia de la observada en estudios previos, mostró diversos grados de polimerización fibrilar, lo que nos induce a sugerir que nuestro modelo puede ser un buen medio de estudiar los mecanismos de reacción en la formación de tejidos reactivos a la lesión dental (AU)

The main goal of this study was the analysis of the developmental potentiality of tooth germ from late bell stage on, after its heterotopic placement within the skin. Teeth germs of newborn rats were grafted within a skin pouch of the ear of adult rats. Seven to fourteen days after grafting, dental germs developed normal dental structures in which ameloblasts and odontoblasts were well differentiated. Twenty to forty-one days after graft, the inflammatory host reaction destroyed the dental developed tissues by cell infiltration. The dentin of the grafts was of osteoid characteristics, and its size increased dependinng on grafting time until the complete substitution of all dental tissues. This atypical dentin showed several degrees of polymerisation from collagen fibres smooth dentin devoid near the graft a to fibres rich dentin far from the dental germ. Present results suggest that this type of dental graft could be a valuable model to study the self-development of dental tissues and the reactive mechanisms taking place after dental injuries (AU)