Intra-articular injection of two different doses of autologous bone marrow mesenchymal stem cells versus hyaluronic acid in the treatment of knee osteoarthritis: multicenter randomized controlled clinical trial (phase I/II).

BACKGROUND: Mesenchymal stromal cells are a promising option to treat knee osteoarthritis. Their safety and usefulness must be confirmed and the optimal dose established. We tested increasing doses of bone marrow mesenchymal stromal cells (BM-MSCs) in combination with hyaluronic acid in a randomized clinical trial. MATERIALS: A phase I/II multicenter randomized clinical trial with active control was conducted. Thirty patients diagnosed with knee OA were randomly assigned to intraarticularly administered hyaluronic acid alone (control), or together with 10 × 10(6) or 100 × 10(6) cultured autologous BM-MSCs, and followed up for 12 months. Pain and function were assessed using VAS and WOMAC and by measuring the knee motion range. X-ray and magnetic resonance imaging analyses were performed to analyze joint damage. RESULTS: No adverse effects were reported after BM-MSC administration or during follow-up. BM-MSC-administered patients improved according to VAS during all follow-up evaluations and median value (IQR) for control, low-dose and high-dose groups change from 5 (3, 7), 7 (5, 8) and 6 (4, 8) to 4 (3, 5), 2 (1, 3) and 2 (0,4) respectively at 12 months (low-dose vs control group p = 0.005 and high-dose vs control group p < 0.009). BM-MSC-administered patients were also superior according to WOMAC, although improvement in control and low-dose patients could not be significantly sustained beyond 6 months. On the other hand, the BM-MSC high-dose group exhibited an improvement of 16.5 (12, 19) points at 12 months (p < 0.01). Consistent with WOMAC and VAS values, motion ranges remained unaltered in the control group but improved at 12 months with BM-MSCs. X-ray revealed a reduction of the knee joint space width in the control group that was not seen in BM-MSCs high-dose group. MRI (WORMS protocol) showed that joint damage decreased only in the BM-MSC high-dose group, albeit slightly. CONCLUSIONS: The single intraarticular injection of in vitro expanded autologous BM-MSCs together with HA is a safe and feasible procedure that results in a clinical and functional improvement of knee OA, especially when 100 × 10(6) cells are administered. These results pave the way for a future phase III clinical trial. CLINICAL TRIALS: gov identifier NCT02123368. Nº EudraCT: 2009-017624-72.

The Relationship Between the Sesamoid Complex and the First Metatarsal After Hallux Valgus Surgery Without Lateral Soft-Tissue Release: A Prospective Study.

Some investigators have emphasized restoring the relationship between the sesamoid complex and the first metatarsal head to reduce the risk of hallux valgus recurring after surgical reconstruction. In a prospective study, we analyzed whether the first metatarsophalangeal joint could be realigned after scarf-Akin bunionectomy without lateral soft tissue release. A total of 25 feet, in 22 patients, were prospectively enrolled and analyzed using anteroposterior radiographs and coronal computed tomography scans obtained before and 3 months after surgery. The Yildirim sesamoid position decreased from a preoperative of 2 (range 1 to 3) to a postoperative position of 0 (range 0 to 1; p < .001), the mean first intermetatarsal angle decreased from 12.6° ± 2.4° to 5.8° ± 2.1° (p < .001), and the mean distance between the second metatarsal and the tibial sesamoid changed from 25.7 ± 4.6 to 25.9 ± 4.6 (p = .59). Our findings suggest that dislocation of the sesamoid complex is actually caused by displacement of the first metatarsal. In conclusion, the scarf-Akin bunionectomy adequately restores the alignment of the first metatarsophalangeal joint, including restoration of the sesamoid apparatus, without direct plantar-lateral soft tissue release.

Imaging colorectal trauma using 64-MDCT technology.

Computed tomography has evolved to become the principal imaging modality in the evaluation of colorectal trauma. Direct signs of colorectal trauma are usually highly specific but relatively insensitive and, therefore, indirect signs are important in the diagnostic of colorectal injuries. In addition, prompt recognition of colorectal injury is crucial as even short delays in its diagnosis have been shown to significantly increase both morbidity and mortality. All these things make colorectal injury a challenge for radiologists. The purpose of this review is to describe and analyze the various computed tomography (CT) findings of colorectal injuries in both penetrating and blunt trauma using 64-multirow detector computed tomography technology, based on our experience in a level I trauma center. Additionally, the CT protocol methods specific to colorectal injuries are detailed.

Struma ovarii maligno no sospechado: diagnóstico citológico mediante punción aspirativa con aguja fina de lesión vertebral metastásica / Unsuspected malignant struma ovarii: cytological diagnosis on fine needle aspiration of a spinal metastatasis

El struma ovarii maligno es un tumor excepcional. A pesar de su rareza, puede dar metástasis igual que un tumor maligno de origen tiroideo. Presentamos un caso, en una paciente mujer de 56 años, que se inició con metástasis pulmonares y óseas. Aportamos un caso único de struma ovarii maligno que se manifestó con metástasis óseas y se diagnosticó mediante citología por punción aspirativa con aguja fina guiada por tomografía computarizada. Queremos enfatizar la utilidad de la inmunohistoquímica, especialmente TTF1 y tiroglobulina, en el material de punción, que permite identificar los nidos epiteliales y microfolículos como tejido tiroideo. Describimos, además, la correlación citohistológica(AU)

Malignant struma ovarii is an exceptionally rare tumour which may metastasize, in the same way as other malignant tumours of thyroid origin. We report a case of a 56-year-old woman who presented with a pelvic mass and lung and bone lesions. To our knowledge this is the first case of malignant struma ovarii with bone metastases diagnosed by CT-guided FNA. The aim of this report is to emphasize the usefulness of immunocytochemistry, in particular TTF1 and thyroglobulin, in cytological samples in order to identify the epithelial cells and microfollicles as thyroid tissue. We also describe the correlation between the cytology and histology of this lesion(AU)