RESUMO
The ability to transfer and implant genetic material continues to have a tremendous impact on the practice of medicine. The techniques involved are constantly being redefined. Gene transfer may be able to ameliorate a number of chronic diseases and has implications in promoting the healing process. We review here the basic approaches involved in gene transfer, including cell-mediated transport, and discuss the implications related to genetic implants.
Assuntos
Técnicas de Transferência de Genes , Terapia Genética/métodos , Transporte Biológico , Vetores Genéticos/metabolismo , Humanos , Veículos Farmacêuticos , Sensibilidade e EspecificidadeRESUMO
The effect of priming and boosting with pspA/EF5668 and purified recombinant PspA/EF5668 was examined. With this strategy CBA/N mice were protected against fatal challenge with Streptococcus pneumoniae EF5668. Anti-PspA antibody titers were elevated, and Western analysis with the immune serum demonstrated cross-reactivity with PspA from several different pneumococcal isolates, representing different PspA clades. Immune serum localized cross-reactive epitopes to the alpha-helical domain of PspA/Rx1 and PspA/EF5668. We demonstrated that DNA/protein prime-boost immunizations can enhance protective immunity against pneumococcal challenge.