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1.
Vet Parasitol Reg Stud Reports ; 45: 100923, 2023 10.
Artigo em Inglês | MEDLINE | ID: mdl-37783532

RESUMO

Point-of-care (POC) ELISA tests are routinely used in US veterinary practices to screen canine patients for antibodies to tick-transmitted pathogens. Results are also used to monitor spatial and temporal trends in canine seroprevalence, and these data can build awareness of the risk to humans of tick-transmitted diseases such as Lyme disease and anaplasmosis. This study utilized a second-generation test that has incorporated additional Anaplasma-specific peptides into a commercial POC ELISA test to allow detection of Anaplasma spp. antibodies earlier post-infection. A convenience population consisting of 19,894 canine samples from a US commercial diagnostic laboratory were tested using the second-generation POC ELISA test to describe regional Anaplasma spp. canine seroprevalence and assess correlation to anaplasmosis cases reported to Centers for Disease Control and Prevention by state. Antibodies to Anaplasma spp. were detected in 1646 samples (8.3%) with the Northeast and Midwest US census regions having the highest proportion of positive samples. At the state level, a significant correlation was found between canine Anaplasma spp. seroprevalence and human anaplasmosis incidence (r2 = 0.64). Although estimates of canine Anaplasma spp. seroprevalence presented here using the second-generation POC ELISA are generally increased, especially in the Northeast and Midwest, the regional distribution of canine samples testing positive for Anaplasma spp. antibodies is consistent with previous reports. The observed correlation with human anaplasmosis incidence indicates that results from the second-generation POC ELISA will continue to add value in epidemiological assessment of human anaplasmosis risk.


Assuntos
Anaplasmose , Borrelia burgdorferi , Dirofilaria immitis , Dirofilariose , Doenças do Cão , Ehrlichiose , Humanos , Cães , Animais , Anaplasmose/epidemiologia , Anaplasma , Estudos Soroepidemiológicos , Incidência , Ehrlichiose/epidemiologia , Ehrlichiose/veterinária , Dirofilariose/epidemiologia , Doenças do Cão/epidemiologia , Anticorpos Antibacterianos
2.
Vet Parasitol ; 324: 110073, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37976897

RESUMO

Intestinal parasites, including cestodes like Dipylidium caninum, are common in dogs in the United States of America (USA), but fecal flotation consistently, and, at times, dramatically, fails to identify many of these infections. To determine the extent to which including coproantigen testing for D. caninum would improve the identification of dogs infected with this cestode, we evaluated fecal samples from 877 dogs (589 pet and 288 from municipal shelters) from six USA states using zinc sulfate (specific gravity 1.24) fecal flotation with centrifugation along with coproantigen detection for Giardia sp., hookworms, ascarids, and Trichuris vulpis. For D. caninum, PCR of perianal swabs was included. Intestinal parasite infections were identified, using centrifugal fecal flotation or coproantigen, in 265 dogs (13.2 % pet, 64.9 % shelter). Dipylidium caninum infection was detected in 5.6 % of dogs with the combination of coproantigen and centrifugal fecal flotation, and 7.3 % of dogs when perianal swab results were included; prevalence varied by diagnostic method, population, and geographic region. In pet dogs, D. caninum infection was identified by fecal flotation (0), coproantigen (2.2 %), or perianal swabs (1.2 %). The same methods revealed infection in 0.3 %, 12.5 %, and 11.1 % of shelter dogs, respectively. Frequent use of praziquantel in shelter dogs (116/288; 40.3 %) may have reduced prevalence. Positive and negative agreement of D. caninum coproantigen with perianal swab PCR in pet dogs was 85.7 % and 98.8 %, respectively. Multiple logistic regression analysis accounting for region, population, and age found D. caninum infection to be more common in shelter dogs relative to pet (adjusted OR 4.91 [2.48, 10.24]) and in the Southcentral and Southeast regions relative to North (adjusted OR 9.59 [1.92, 174.13] and 17.69 [3.67, 318.09] respectively). Coproantigen testing also enhanced the detection of other intestinal parasites over fecal flotation alone, including Giardia sp. (14.7 % vs 3.3 %), hookworms (13.8 % vs 8.4 %), ascarids (2.9 % vs 2.2 %), and T. vulpis (2.9 % vs 1.4 %). Together, these data indicate that the coproantigen assay employed increases detection of D. caninum infections several fold, supporting the use of this test in clinical practice, and add to a growing body of research documenting enhanced diagnosis through implementation of multiple laboratory-based methods.


Assuntos
Infecções por Cestoides , Doenças do Cão , Enteropatias Parasitárias , Parasitos , Animais , Cães , Enteropatias Parasitárias/diagnóstico , Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/veterinária , Infecções por Cestoides/diagnóstico , Infecções por Cestoides/epidemiologia , Infecções por Cestoides/veterinária , Trichuris , Giardia , Fezes/parasitologia , Prevalência , Doenças do Cão/diagnóstico , Doenças do Cão/epidemiologia , Doenças do Cão/parasitologia
3.
J Vet Intern Med ; 36(2): 532-540, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35132685

RESUMO

BACKGROUND: Infection with Bartonella species is common in cats but reported effects of bacteremia on laboratory variables differ. OBJECTIVES: Evaluate for associations between Bartonella bacteremia and CBC and serum biochemical changes in sick and healthy cats throughout the United States. ANIMALS: A total of 3964 client-owned cats. METHODS: Retrospective cohort study using submissions to a commercial laboratory between 2011 and 2017. Serum biochemistry and CBC abnormalities (categorized as above or below reference intervals), age, and location (high- or low-risk state for Ctenocephalides felis) in presumed healthy and sick cats were evaluated for associations with presence of Bartonella spp. DNA, detected by PCR. Univariate and multivariable logistic regression analyses were performed. RESULTS: Bartonella spp. DNA was amplified from 127 (3.2%) of 3964 cats; 126 (99.2%) of 127 were from high flea risk states and 121 (95.3%) of 127 were presumed sick. Fever of unknown origin was the most common PCR panel requested. In the multivariable analysis, neutrophilia, decreased ALP activity, clinical status (presumed sick), and young age (≤2 years) each were positively associated whereas neutropenia and hyperproteinemia both were negatively associated with Bartonella spp. bacteremia. Presence of Bartonella spp. DNA had no association with test results for other infectious disease agents. CONCLUSIONS AND CLINICAL IMPORTANCE: In both healthy and sick cats, active Bartonella infections had minimal association with clinically relevant laboratory abnormalities. However, based on these results, in areas considered high risk for C. felis, active infection with Bartonella spp. is a reasonable differential diagnosis for cats presented with unexplained fever and neutrophilia, particularly if the cat is young.


Assuntos
Infecções por Bartonella , Bartonella , Doenças do Gato , Animais , Bartonella/genética , Infecções por Bartonella/veterinária , Contagem de Células Sanguíneas/veterinária , Gatos , DNA , Humanos , Estudos Retrospectivos
4.
Parasit Vectors ; 14(1): 10, 2021 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-33407758

RESUMO

BACKGROUND: Dogs in the US are commonly infected with vector-borne pathogens, including heartworm and tick-borne disease agents. The geographic distribution of both arthropod vectors and the pathogens they transmit continues to expand. METHODS: To describe the current geographic distribution and prevalence of antigen of Dirofilaria immitis and antibody to Borrelia burgdorferi, Ehrlichia spp., and Anaplasma spp. in dogs, we summarized over 144 million test results from 2013 to 2019, inclusive, by county, state, and region. Canine seroprevalence by state was compared to population-adjusted human reports of tick-borne diseases. RESULTS: Results varied regionally, with D. immitis antigen and Ehrlichia spp. antibodies more frequently detected in the Southeast (2.6% and 5.2%, respectively) and antibody to B. burgdorferi and Anaplasma spp. most common in the Northeast (12.1% and 7.3%, respectively). Overall, percent positive test results to D. immitis decreased in the Southeast by 33.3% when compared to earlier summaries using the same strategy (from 3.9 to 2.6%). Geographic expansion of areas where dogs commonly test positive for Ehrlichia spp. was evident, likely because of a change in the test made in 2012 to allow detection of antibodies to E. ewingii concomitant with expansion of vector tick populations. Percent positive test results to Ehrlichia spp. increased in every region; this shift was particularly pronounced in the Southeast, where percent positive test results increased fourfold (from 1.3 to 5.2%). Continued geographic expansion of B. burgdorferi and A. phagocytophilum was apparent in the Northeast, Midwest, and Upper South, although canine seroprevalence of antibody to B. burgdorferi was much lower than prior surveys in many Lyme-endemic areas. Annual reports of human cases of Lyme disease, ehrlichiosis, and anaplasmosis were associated with percent positive canine results by state for the three tick-borne disease agents (R2 = 0.812, 0.521, and 0.546, respectively). Within endemic areas, percent positive test results for all three tick-borne agents demonstrated evidence of geographic expansion. CONCLUSIONS: Large scale analysis of results from screening dogs in practice for evidence of vector-borne infections, including those with zoonotic importance, continues to be a valuable strategy for understanding geographic trends in infection risk over time.


Assuntos
Anaplasma , Borrelia burgdorferi , Dirofilaria immitis , Cães , Ehrlichia , Doenças Transmitidas por Carrapatos/veterinária , Anaplasma/imunologia , Anaplasma/isolamento & purificação , Anaplasmose/epidemiologia , Animais , Anticorpos Antibacterianos/sangue , Anticorpos Anti-Helmínticos/sangue , Antígenos de Helmintos/sangue , Borrelia burgdorferi/imunologia , Borrelia burgdorferi/isolamento & purificação , Dirofilaria immitis/imunologia , Dirofilaria immitis/isolamento & purificação , Dirofilariose/epidemiologia , Doenças do Cão/epidemiologia , Cães/microbiologia , Cães/parasitologia , Ehrlichia/imunologia , Ehrlichia/isolamento & purificação , Ehrlichia canis/imunologia , Ehrlichia canis/isolamento & purificação , Ehrlichiose/epidemiologia , Ehrlichiose/veterinária , Humanos , Doença de Lyme/epidemiologia , Doença de Lyme/veterinária , Prevalência , Estudos Retrospectivos , Estudos Soroepidemiológicos , Doenças Transmitidas por Carrapatos/epidemiologia , Estados Unidos/epidemiologia , Doenças Transmitidas por Vetores/microbiologia , Doenças Transmitidas por Vetores/parasitologia , Doenças Transmitidas por Vetores/veterinária , Zoonoses/microbiologia , Zoonoses/parasitologia
5.
Vet Parasitol Reg Stud Reports ; 22: 100471, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33308727

RESUMO

Babesia species are important canine pathogens with a nearly worldwide distribution. Our understanding of the distribution of these parasites is continually improving. This is in large part, due to improved molecular diagnostic capabilities. However, it can be difficult to assimilate and compare previous reports from various regions due to differences in molecular methods. In this report, we characterize the results of over 100,000 canine samples from 52 different countries and territories spanning 4 continents that were submitted to a commercial diagnostic laboratory for Babesia testing by polymerase chain reaction. The same diagnostic algorithm was used for all samples and is designed to identify and differentiate B. gibsoni, B. canis, B. vogeli, B. rossi and B. conradae. Overall 3.4% of the samples submitted tested positive for the presence of Babesia sp. DNA and were differentiated to the species level. Babesia gibsoni was the most commonly identified species (48.8% of the positive results) followed by B. canis (35.2%) then B. vogeli (15.3%). Babesia gibsoni and B. vogeli were more widely distributed than B. canis, which was primarily found in Europe. This is the largest study of its type and these data provide a global overview of which Babesia species veterinarians could expect to find in their practice area.


Assuntos
Babesia , Babesiose , Doenças do Cão , Cães/parasitologia , Animais , Babesia/classificação , Babesiose/diagnóstico , Babesiose/epidemiologia , Doenças do Cão/diagnóstico , Doenças do Cão/epidemiologia , Doenças do Cão/parasitologia , Laboratórios , Reação em Cadeia da Polimerase/veterinária
6.
Pathogens ; 9(6)2020 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-32580453

RESUMO

In the last two decades, reports of canine heartworm (HW) infection have increased even in non-endemic areas, with a large variability in prevalence data due to the diagnostic strategy employed. This study evaluated the relative performance of two microtiter plate ELISA methods for the detection of HW antigen in determining the occurrence of Dirofilaria immitis in a dog population previously tested by the modified Knott's test and SNAP 4Dx Plus test. The prevalence of this infection in the sheltered dog population (n = 363) from a high-risk area for HW infection was 44.4% according to the modified Knott's test and 58.1% according to a point-of-care antigen ELISA. All serum samples were then evaluated by a microtiter plate ELISA test performed with and without immune complex dissociation (ICD). The prevalence increased from 56.5% to 79.6% following ICD, indicating a high proportion of samples with immune complexing. Comparing these results to that of the modified Knott's test, the samples negative for microfilariae (mfs) and those positive only for D. repens mfs demonstrated the greatest increase in the proportion of positive results for D. immitis by ELISA following ICD. While the ICD method is not recommended for routine screening, it may be a valuable secondary strategy for identifying HW infections in dogs.

7.
Appl Environ Microbiol ; 75(16): 5345-55, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19542347

RESUMO

The deep sea (water depth of >2,000 m) represents the largest biome on Earth. Yet relatively little is known about its microbial community's structure, function, and adaptation to the cold and deep biosphere. To provide further genomic insights into deep-sea planktonic microbes, we sequenced a total of approximately 200 Mbp of a random whole-genome shotgun (WGS) library from a microbial community residing at a depth of 4,000 m at Station ALOHA in the Pacific Ocean and compared it to other available WGS sequence data from surface and deep waters. Our analyses indicated that the deep-sea lifestyle is likely facilitated by a collection of very subtle adaptations, as opposed to dramatic alterations of gene content or structure. These adaptations appear to include higher metabolic versatility and genomic plasticity to cope with the sparse and sporadic energy resources available, a preference for hydrophobic and smaller-volume amino acids in protein sequences, unique proteins not found in surface-dwelling species, and adaptations at the gene expression level. The deep-sea community is also characterized by a larger average genome size and a higher content of "selfish" genetic elements, such as transposases and prophages, whose propagation is apparently favored by more relaxed purifying (negative) selection in deeper waters.


Assuntos
Archaea , Bactérias , Ecossistema , Genômica , Água do Mar/microbiologia , Archaea/classificação , Archaea/genética , Proteínas Arqueais/genética , Bactérias/classificação , Bactérias/genética , Proteínas de Bactérias/genética , Biologia Computacional , Biblioteca Genômica , Havaí , Redes e Vias Metabólicas , Dados de Sequência Molecular , Oceano Pacífico , Plâncton/classificação , Plâncton/genética , Análise de Sequência de DNA
8.
JFMS Open Rep ; 5(2): 2055116919869053, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31523446

RESUMO

OBJECTIVES: The aim of this study was to compile commercial reference laboratory data over a 10-year period to determine the distribution of Aelurostrongylus abstrusus, commonly known as feline lungworm, within the USA based on widespread fecal testing in cats. METHODS: The results of 3,610,455 feline ova and parasite (O&P) zinc sulfate centrifugation fecal flotation tests performed at IDEXX Reference Laboratories in the USA from January 2008 to December 2017 were compiled and sorted for tests positive for A abstrusus larvae. The results of 3625 Baermann tests, currently considered the gold standard diagnostic for feline lungworm, were also retrieved from the same period. RESULTS: Of the tests performed, 4721 (0.13%) feline O&P zinc sulfate centrifugation fecal flotation tests and 75 (2.07%) of the Baermann tests conducted were positive for the presence of A abstrusus larvae. The O&P data revealed a significant association between infection status and sex, while male cats in both the O&P and Baermann data sets had a higher risk of A abstrusus infection than females. Significant variation in positive rates were observed by region and most positive cases were clustered in the Northeast, Midwest and West regions of the USA. CONCLUSIONS AND RELEVANCE: This study highlights the distribution of feline lungworm in the USA and the limitations of using current testing to diagnose this infection. The introduction of higher throughput, less labor-intensive diagnostic methods could help increase awareness of this parasite among veterinary professionals, achieve a greater understanding of epidemiological factors, and improve the care and treatment for clinically ill feline patients.

9.
Parasit Vectors ; 12(1): 476, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31610803

RESUMO

BACKGROUND: Borrelia burgdorferi and Anaplasma phagocytophilum are tick-borne infections transmitted by Ixodes scapularis in the eastern USA; both agents cause disease in dogs and people. To characterize changes in seroprevalence over time, Cochran Armitage trend tests were used to evaluate percent positive test results for antibodies to B. burgdorferi and Anaplasma spp. in approximately 20 million canine tests from 2010-2017 in 25 states and 905 counties in the eastern USA. RESULTS: A significant decreasing trend in seroprevalence to B. burgdorferi was evident in eight states along the mid-Atlantic coast from Virginia to New Hampshire, and in Wisconsin. In contrast, a continued increasing trend was evident in five northeastern and Midwestern states where Lyme borreliosis is endemic or emerging, as well as in three southern states where endemicity has not yet been widely established. Similarly, seroprevalence to Anaplasma spp. showed a significant, although smaller, decreasing trend in five states along the mid-Atlantic coast from Virginia to Connecticut and Rhode Island, as well as in Minnesota and Wisconsin in the Midwest; despite the fact that those trends were significant they were weak. However, a strong increasing trend was evident in Massachusetts and three states in northern New England as well as in Pennsylvania. CONCLUSIONS: As expected, seroprevalence continued to increase in regions where Lyme borreliosis and anaplasmosis are more newly endemic. However, the declining seroprevalence evident in other areas was not anticipated. Although the reasons for the decreasing trends are not clear, our finding may reflect shifting ecologic factors that have resulted in decreased infection risk or the combined positive influence of canine vaccination, tick control, and routine testing of dogs in regions where these infections have long been endemic. Analysis of trends in canine test results for tick-borne infections continues to be a valuable tool to understand relative geographical and temporal risk for these zoonotic agents.


Assuntos
Anaplasma/imunologia , Anaplasmose/epidemiologia , Borrelia burgdorferi/imunologia , Doenças do Cão/epidemiologia , Ehrlichiose/epidemiologia , Doença de Lyme/veterinária , Anaplasma phagocytophilum/imunologia , Animais , Anticorpos Antibacterianos/sangue , Doenças do Cão/microbiologia , Cães , Doenças Endêmicas/estatística & dados numéricos , Doenças Endêmicas/veterinária , Doença de Lyme/epidemiologia , Estudos Soroepidemiológicos , Estados Unidos/epidemiologia
10.
Environ Microbiol Rep ; 1(6): 524-34, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23765931

RESUMO

Carotenoids are isoprenoid pigments synthesized in plants, fungi, bacteria and archaea, with roles in light harvesting, protection from stress, and membrane and protein structures. To characterize carotenoid biosynthesis genes from oceanic microbes, a fosmid library derived from microbial samples collected in surface water of the Pacific Ocean was screened in Escherichia coli for pigment-expressing recombinant strains. One DNA fragment enabled production of a bright orange pigment, and was analysed further by sequence analysis and phenotypic characterization. The cloned DNA encoded a five-gene cluster predicted to be involved in the synthesis of canthaxanthin, a ketolated carotenoid. Each of these genes was inactivated by insertion of a transposon, and the biochemical function of each gene product was confirmed. Sequencing of related fosmids generated a 67 kb genomic contig, and comparative analyses suggested that the DNA may originate from a deltaproteobacterium. The carotenoid biosynthesis genes described here are related to well-characterized families of carotenoid biosynthesis genes, but also indicate that the organism harbouring them is only distantly related to any previously characterized bacterial types.

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