RESUMO
In the disseminated form of histoplasmosis, isolation and further identification of Histoplasma capsulatum can be performed by several methods, namely, bone marrow aspiration, blood culture, and liver biopsy. Lymph node disease usually is diagnosed by excisional biopsy. Although fungal stains can identify this fungus, detection of specific antigens by immunohistochemistry shows a higher specificity and sensitivity. This approach can use the cell block method when the material is not sent to fungal cultures or fresh staining.
Assuntos
Biópsia por Agulha Fina , Histoplasmose/diagnóstico , Linfadenite/microbiologia , Antígenos de Fungos/análise , Histoplasma/imunologia , Histoplasmose/microbiologia , Histoplasmose/patologia , Humanos , Técnicas Imunoenzimáticas , Masculino , Pessoa de Meia-Idade , PescoçoRESUMO
El aislamiento y la posterior identificación de Histoplasma capsulatum en lahistoplasmosis diseminada puede llevarse a cabo por diversos métodos,como la aspiración de médula ósea, el hemocultivo o la biopsia de hígado.La linfadenopatía es habitualmente diagnosticada por extirpación del ganglioafectado. Aunque la tinción del hongo puede llevar a su identificación,la detección de antígenos específicos mediante procedimientos deinmunohistoquímica muestra una mayor sensibilidad y especificidad.Este método permite la fijación de las células cuando el material no va a serprocesado para cultivo micológico o tinción en fresco(AU)
In the disseminated form of histoplasmosis, isolation and further identificationof Histoplasma capsulatum can be performed by several methods, namely,bone marrow aspiration, blood culture, and liver biopsy. Lymph node diseaseusually is diagnosed by excisional biopsy. Although fungal stains can identifythis fungus, detection of specific antigens by immunohistochemistry shows ahigher specificity and sensitivity. This approach can use the cell block methodwhen the material is not sent to fungal cultures or fresh staining(AU)