Treatment with hyperbaric oxygen in a Crohn`s disease patient.

Despite advances in the treatment of inflammatory bowel disease, particularly with biological therapies and new small molecules, a significant gap still exists in achieving persistent remission from a symptomatic, biomarker, and endoscopic perspective. In this context, hyperbaric oxygen therapy (HBOT) is considered as a therapeutic strategy. This approach has also been suggested for managing ischemic ulcers located at anastomotic sites. In this clinical case, we describe the clinical and endoscopic evolution of a challenging-to-manage Crohn's disease (CD) patient with an ischemic ulcer at the ileo-rectal anastomosis who underwent HBOT.

Personalised therapy in inflammatory bowel disease. / Terapia personalizada en la enfermedad inflamatoria intestinal.

Inflammatory bowel diseases (IBD), with ulcerative colitis and Crohn's disease being their most common presentations, comprise a spectrum of diverse disease phenotypes, exhibiting variable behaviors ranging from an indolent course to aggressive phenotypes that impact quality of life of these patients. The last two decades have been marked by the development of new medications (biological therapy and novel small molecules) with diverse mechanisms of action, which have revolutionized the management of IBD, thereby enhancing the quality of life for these patients. This landscape of multiple therapeutic options underscores the need to define which medication will benefit each patient the most and at what speed it should be started. The objective of this review is to present personalized approaches for patients with IBD, thus contributing to therapeutic management.

Evaluation of the sensitivity and specificity of sigmoidoscopy in comparison to colonoscopy regarding the detection of intestinal inflammatory activity in the follow-up of patients with ulcerative colitis. / Evaluación de la sensibilidad y especificidad de la sigmoidoscopia en comparación con la colonoscopia en la detección de actividad inflamatoria intestinal en el seguimiento de pacientes con colitis ulcerosa.

INTRODUCTION: Ulcerative colitis (UC) is a chronic disease characterized by periods of inflammatory activity and remission, which vary from the rectum to the proximal colon. Currently, mucosal healing is a long-term goal in the management of inflammatory bowel disease, with colonoscopy and sigmoidoscopy being the recommended tools for evaluation. OBJECTIVE: To assess the effectiveness of both examinations in determining the presence of inflammatory activity in the follow-up of patients with UC. METHODS: Retrospective observational study analyzing colonoscopies performed as part of the follow-up of UC patients between January 2021 and July 2023 by gastroenterologists from the Inflammatory Bowel Disease Program at the Clínica Universidad de los Andes. The study compared endoscopic and histological activity observed in the rectosigmoid region with that found in the rest of the colon. Sensitivity and specificity were determined using concordance and correlations tests. RESULTS: A very good concordance and correlation were observed regarding endoscopic findings, with a Kappa index of 0.97 and a Spearman coefficient of 0.97. The Positive Predictive Value (PPV) of sigmoidoscopy for endoscopic activity was 1, and the Negative Predictive Value (NPV) was 0.96. In relation to histological activity, the concordance had a Kappa index of 0.93 and a Spearman coefficient of 0.93, with a PPV of sigmoidoscopy for histological activity being 1 and an NPV of 0.91. CONCLUSION: This cohort suggests that sigmoidoscopy is a cost-effective option for evaluating mucosal healing in UC patients in symptomatic and biomarker remission. However, complete colonoscopy should be considered in cases of discrepancies with the clinical picture or in colorectal cancer surveillance.

Vancomycin in the treatment of inflammatory bowel disease: there is a role beyond Clostridioides difficile infection.

The development of new biological agents and small molecules has revolutionized the treatment of Inflammatory Bowel Disease (IBD). However, many patients do not respond or gradually lose their response, necessitating the search for other therapeutic strategies (1). In this clinical case, we describe the evolution of a patient with difficult-to-manage Crohn's Disease (CD) who was treated with oral vancomycin.

The putative phosphate transporter PitB (PP1373) is involved in tellurite uptake in Pseudomonas putida KT2440.

Tellurium oxyanions are chemical species of great toxicity and their presence in the environment has increased because of mining industries and photovoltaic and electronic waste. Recovery strategies for this metalloid that are based on micro-organisms are of interest, but further studies of the transport systems and enzymes responsible for implementing tellurium transformations are required because many mechanisms remain unknown. Here, we investigated the involvement in tellurite uptake of the putative phosphate transporter PitB (PP1373) in soil bacterium Pseudomonas putida KT2440. For this purpose, through a method based on the CRISPR/Cas9 system, we generated a strain deficient in the pitB gene and characterized its phenotype on exposing it to varied concentrations of tellurite. Growth curves and transmission electronic microscopy experiments for the wild-type and ΔpitB strains showed that both were able to internalize tellurite into the cytoplasm and reduce the oxyanion to black nano-sized and rod-shaped tellurium particles, although the ΔpitB strain showed an increased resistance to the tellurite toxic effects. At a concentration of 100 µM tellurite, where the biomass formation of the wild-type strain decreased by half, we observed a greater ability of ΔpitB to reduce this oxyanion with respect to the wild-type strain (~38 vs ~16 %), which is related to the greater biomass production of ΔpitB and not to a greater consumption of tellurite per cell. The phenotype of the mutant was restored on over-expressing pitB in trans. In summary, our results indicate that PitB is one of several transporters responsible for tellurite uptake in P. putida KT2440.

[Body image, psychological symptoms and eating disorders among Chilean adolescents and young adults]. / Comparación por sexo en imagen corporal, síntomas psicopatológicos y conductas alimentarias en jóvenes entre 14 y 25 años.

UNLABELLED: The construction of body image is crucial during adolescent development. Several studies show that body dissatisfaction is common, especially among women. This is a risk factor for eating behavior disorders. AIM: To describe psychological variables and dimensions about body image among adolescents and young adults. MATERIAL AND METHODS: Three self-administered questionnaires, MBSRQ (Multidimensional Body Self Relations Questionnaire) that measures body image, Symptom Checklist (SCL-90) that measures the presence of psychological and psychiatric symptoms and the Eating Disorder Inventory (EDI-2), which measures eating problems, were applied to 1,438 students aged 19 ± 2.7 years (53% women) from three Chilean regions. RESULTS: Sixty five percent of respondents would like to weigh less. Compared with men, women have greater psychological distress, concerns about their appearance and their weight, are more obsessed with thinness, and have fewer behaviors aimed at solving these problems. CONCLUSIONS: A high percentage of respondents want to lose weight. In addition, women have serious desires and search for thinness.

Gut microbiota DPP4-like enzymes are increased in type-2 diabetes and contribute to incretin inactivation.

BACKGROUND: The gut microbiota controls broad aspects of human metabolism and feeding behavior, but the basis for this control remains largely unclear. Given the key role of human dipeptidyl peptidase 4 (DPP4) in host metabolism, we investigate whether microbiota DPP4-like counterparts perform the same function. RESULTS: We identify novel functional homologs of human DPP4 in several bacterial species inhabiting the human gut, and specific associations between Parabacteroides and Porphyromonas DPP4-like genes and type 2 diabetes (T2D). We also find that the DPP4-like enzyme from the gut symbiont Parabacteroides merdae mimics the proteolytic activity of the human enzyme on peptide YY, neuropeptide Y, gastric inhibitory polypeptide (GIP), and glucagon-like peptide 1 (GLP-1) hormones in vitro. Importantly, administration of E. coli overexpressing the P. merdae DPP4-like enzyme to lipopolysaccharide-treated mice with impaired gut barrier function reduces active GIP and GLP-1 levels, which is attributed to increased DPP4 activity in the portal circulation and the cecal content. Finally, we observe that linagliptin, saxagliptin, sitagliptin, and vildagliptin, antidiabetic drugs with DPP4 inhibitory activity, differentially inhibit the activity of the DPP4-like enzyme from P. merdae. CONCLUSIONS: Our findings confirm that proteolytic enzymes produced by the gut microbiota are likely to contribute to the glucose metabolic dysfunction that underlies T2D by inactivating incretins, which might inspire the development of improved antidiabetic therapies.

Influence of Body Image, Risk of Eating Disorder, Psychological Characteristics, and Mood-Anxious Symptoms on Overweight and Obesity in Chilean Youth.

This study investigates the relationship between body image, eating disorders, psychological characteristics, and mood and anxiety symptoms in Chilean youth, with nutritional status, particularly overweight and obesity. With a sample of 1001 participants from five regions of Chile, aged 15 to 23 years. The Eating Disorder Inventory 3 (EDI-3), the Multidimensional Body-Self Relations Questionnaire Appearance Scales (MBSRQ_AS), and the Symptom Inventory Derogatis Revised (SCL90-R) and a sociodemographic questionnaire were used to analyze these variables. A model including nine exogenous (independent) variables and ten endogenous variables, based on a literature review, was evaluated by path analysis. The results show a significant association between factors such as sex, family history of overweight, self-classification by weight, and body dissatisfaction with body mass index (BMI). Eating behaviors such as overeating, and lack of appetite were also found to be influenced by interpersonal sensitivity, overweight preoccupation, and drive for thinness. The study underscores the importance of promoting a positive body image and addressing overweight/obesity from a combined health psychology and public health perspective, highlighting the need for interventions that consider nutritional status, and in particular overweight and obesity, as a phenomenon with multifactorial causes and maintainers.

The microbiota composition drives personalized nutrition: Gut microbes as predictive biomarkers for the success of weight loss diets.

The investigation of the human gut microbiome during recent years has permitted us to understand its relevance for human health at a systemic level, making it possible to establish different functional axes (e.g., the gut-brain, gut-liver, and gut-lung axes), which support the organ-like status conferred to this microecological component of our body. The human gut microbiota is extremely variable but modifiable via diet, a fact that allows targeting of microbes through defined dietary strategies to uncover cost-effective therapies to minimize the burden of non-communicable diseases such as pandemic obesity and overweight and its metabolic comorbidities. Nevertheless, randomly controlled dietary interventions regularly exhibit low to moderate degrees of success in weight control, making their implementation difficult in clinical practice. Here, we review the predictive value of the baseline gut microbiota configurations to anticipate the success of dietary interventions aimed at weight loss, mostly based on caloric restriction regimes and oral fiber supplementation. This emergent research concept fits into precision medicine by considering different diet patterns and adopting the best one, based on the individual microbiota composition, to reach significant adiposity reduction and improve metabolic status. We review the results from this fresh perspective of investigation, taking into account studies released very recently. We also discuss some future outlooks in the field and potential pitfalls to overcome with the aim of gaining knowledge in the field and achieving breakthroughs in personalized nutrition.

[Parental feeding strategies in children with Avoidant/Restrictive Food Intake Disorder (ARFID): Association with food refusal behavior]. / Estrategias parentales de alimentación en niños con un Trastorno de Evitación/Restricción de la Ingesta de Alimentos (ARFID): Asociación con la conducta de rechazo a los alimentos.

Avoidance/Restriction of Food Intake Disorder (ARFID) is characterized by persistent avoidance and/or restriction of food intake, with three clinical presentations: lack of interest in food, selectivity based on sensory sensitivity, and fear of aversive consequences. The strategies used by parents during mealtimes may predispose or maintain the child's food refusal. OBJECTIVE: to determine the associa tion between parental strategies used during mealtimes and food refusal behavior in ARFID children. PATIENTS AND METHOD: Cross-sectional research. Non-probabilistic sampling was used for the selec tion of participants. Twenty-four parents whose children had been diagnosed with ARFID participa ted. ARFID subtypes were considered according to DSM-5 criteria: limited appetite, selective eating and fear of eating. Exclusion criteria were ARFID of organic cause and/or pervasive developmental disorders. The Child Eating Behaviors Questionnaire and the Child Feeding Questionnaire were used for data collection. RESULTS: An association was evidenced between the parental eating strategy of pressure to eat with food refusal behavior, and with child eating behaviors of emotional underfeeding (p = 0.046), slowness to eat (p = 0.016), refusal in front of food (p = 0.019) and satiety response (p = 0.003). CONCLUSION: Eating behaviors frequently perceived by parents with children diagnosed with ARFID are related to the dimension of negative approach towards food, such as satiety response, food refusal, slowness to eat and emotional underfeeding.

Control of nuclear size by osmotic forces in Schizosaccharomyces pombe.

The size of the nucleus scales robustly with cell size so that the nuclear-to-cell volume ratio (N/C ratio) is maintained during cell growth in many cell types. The mechanism responsible for this scaling remains mysterious. Previous studies have established that the N/C ratio is not determined by DNA amount but is instead influenced by factors such as nuclear envelope mechanics and nuclear transport. Here, we developed a quantitative model for nuclear size control based upon colloid osmotic pressure and tested key predictions in the fission yeast Schizosaccharomyces pombe. This model posits that the N/C ratio is determined by the numbers of macromolecules in the nucleoplasm and cytoplasm. Osmotic shift experiments showed that the fission yeast nucleus behaves as an ideal osmometer whose volume is primarily dictated by osmotic forces. Inhibition of nuclear export caused accumulation of macromolecules in the nucleoplasm, leading to nuclear swelling. We further demonstrated that the N/C ratio is maintained by a homeostasis mechanism based upon synthesis of macromolecules during growth. These studies demonstrate the functions of colloid osmotic pressure in intracellular organization and size control.

Psychometric Properties of the Eating Disorder Inventory-3 (EDI-3) in Chilean Youth.

The aim of this study was to analyze the psychometric properties of the Eating Disorder Inventory (EDI)-3 test to evaluate eating disorders in young Chilean population. Methods: The sample consisted of 1,091 Chilean adolescents and young people (i.e., 476 men and 615 women) between 15 and 28 years old, from the metropolitan region, and four regions from the coast and south-central zone of the country. The reliability and factorial structure of the instrument were analyzed, replicating the confirmatory factor analyses of Brookings et al. (2020), evaluating four additional models that included bifactor exploratory structural equation modeling (ESEM), bifactor, and two-bifactor. Results: A majority of the subscales presented alphas and omegas equal to or greater than 0.70, with the exception of asceticism (α = 0.543, ω = 0.552) and interpersonal alienation (α = 0.684, ω = 0.695) scales, which are consistent with the values of the Spanish and Mexican non-clinical samples. The best fit indices were obtained by the ESEM two-bifactor model, with twelve specific factors corresponding to the EDI-3 subscales and two general orthogonal factors (i.e., risk subscales and psychological subscales), consistently with the theoretical basis.

Psychometric Properties of the Multidimensional Body-Self Relations Questionnaire-Appearance Scales (MBSRQ-AS) in Chilean Youth.

The aim of this study is to analyze the factorial structure of the Multidimensional Body-Self Relations Questionnaire-Appearance Scales (MBSRQ-AS) to determine the adjustment of the study structure proposed in the Spanish short version in a young, non-clinical Chilean population and to evaluate the possible influence of sex, age, and BMI on body image measurement. The sample consisted of 614 Chilean youth (259 male and 355 female) between 15 and 28 years old (M = 18.81; SE = 2.46), from the Metropolitan Region, and four regions of the coast and south-central zone of the country. The average Body Mass Index (BMI) was 22.5 kg/m2 (SD = 3.16). The model fit was evaluated by confirmatory factor analysis (WLSMV) using the following: a model with a single general factor, a model with the five factors of the original version, a five-factor ESEM model, and a MIMIC model analyzed including sex, age, and BMI. The results show that the MIMIC sex, age, and BMI model presents an acceptable fit, observing that four factors, Appearance Orientation (AO), Body Areas Satisfaction (BAS), Overweight Preoccupation (OP), and Self-Classification Weight (SCW), are affected significantly for the sex variable, one for age, Appearance Evaluation (AE), and four for BMI (AE, BAS, OP, and SCW). In conclusion, MBSRQ-AS replicates the five-dimensional structure in a non-clinical sample of young Chileans; however, their scores are not invariant as they depend on sex, age, and BMI.

Physical properties of the cytoplasm modulate the rates of microtubule polymerization and depolymerization.

The cytoplasm is a crowded, visco-elastic environment whose physical properties change according to physiological or developmental states. How the physical properties of the cytoplasm impact cellular functions in vivo remains poorly understood. Here, we probe the effects of cytoplasmic concentration on microtubules by applying osmotic shifts to fission yeast, moss, and mammalian cells. We show that the rates of both microtubule polymerization and depolymerization scale linearly and inversely with cytoplasmic concentration; an increase in cytoplasmic concentration decreases the rates of microtubule polymerization and depolymerization proportionally, whereas a decrease in cytoplasmic concentration leads to the opposite. Numerous lines of evidence indicate that these effects are due to changes in cytoplasmic viscosity rather than cellular stress responses or macromolecular crowding per se. We reconstituted these effects on microtubules in vitro by tuning viscosity. Our findings indicate that, even in normal conditions, the viscosity of the cytoplasm modulates the reactions that underlie microtubule dynamic behaviors.

Acute Heat Stress Leads to Reversible Aggregation of Nuclear Proteins into Nucleolar Rings in Fission Yeast.

Upon acute heat stress (HS), overall mRNA transcription, processing, and export are inhibited, leading to cell growth arrest. However, how cells turn off mRNA metabolism is not fully understood. Here, we show that acute HS results in the segregation and aggregation of multiple nuclear and nucleolar proteins into ring-like structures located at the nucleolar periphery (nucleolar rings [NuRs]). NuRs sequester essential factors required for nuclear mRNA metabolism and nuclear pore complex function, as well as cell-cycle regulators. When cells are switched back to growing temperatures, NuRs disaggregate, and their components relocate to their functional environments in an Hsf1- and Hsp104-dependent manner, and concomitantly with the reinitiation of cell growth. These findings highlight the contribution of reversible protein aggregation to the inhibition of overall RNA-related activities in the nucleus and its functional relevance in the maintenance of cellular homeostasis during acute HS.

Copro-prevalence of Fasciola hepatica in Chilean breed horses in the province of Concepción, Chile.

The aim of this research was to assess the copro-prevalence of Fasciola hepatica in owned Chilean breed horses (Equus caballus) residing in the province of Concepción, Chile. The study was carried out throughout October 2017. Samples were taken from all (100%) Chilean breed horse (124 specimens; 45 females and 79 males, aged between 7 months and 24 years old) from the Concepción province. A rectal stool sample was obtained from each animal. This was analyzed using the Army Medical School method (AMS III) technique to detect F. hepatica eggs. Information about the horse's age, sex, previous fasciolicide application, and an assessment of body condition was also obtained. Fisher tests were carried out to analyze the results. Ten horses (8.06%) were positive for the presence of F. hepatica eggs. No significant difference of copro-prevalence was found between age (young 8.47%, old 7.69%), sex (female 11.1%, male 6.33%), the previous application of fasciolicide (dewormed 10%, not dewormed 7.89%), or body condition (low condition 11.1%, high condition 0.7%) categories (Fisher tests: P>0.05 in all cases). The results of this study suggest that Chilean breed horses constitute part of the reservoir of F. hepatica for both ruminant populations and humans in the Concepción province.

[Differences in body image of young normal weight and overweight/obese people]. / Diferencias en imagen corporal de jóvenes con normopeso y con sobrepeso/obesidad.

INTRODUCTION: Obesity is a chronic and growing problem. Its study requires the understanding of multiple associated variables. Body image has an important role in the health condition. The purpose of this study was to compare the perception of body image of young normal weight and overweight/obese people. MATERIAL AND METHODS: A sample of 376 young men and women aged 18 to 25 was compared. Body mass index classified them in two groups: normal weight and overweight/obese. To evaluate body image participants answered the Multidimensional Body Self Relations Questionnaire (MBSRQ). To evaluate eating behavior they answered the Eating Disorders Inventory (EDI-3). RESULTS: Overweight/obese young people are less satisfied with their appearance and their body when it is evaluated separately. They evaluate their physical condition and their physical abilities badly. They show fewer behaviors intended to maintain or improve their physical appearance, perceive a worse diagnosis of their health and show less body satisfaction. They are more concerned about their weight and they are obsessed about thinness. In addition, women, compared to men, have a worse negative assessment of their body image. CONCLUSION: Despite the low assessment that overweight/obese students made in relation to their body, they are not willing to perform activities that allow them to change their condition. Further research is needed in order to develop preventive programs and more treatments that are effective.

INTRODUCCIÓN: la obesidad es un problema crónico y en aumento. Su estudio requiere comprender múltiples variables asociadas. La imagen corporal tiene un papel importante en esta condición de salud. El propósito de este estudio fue comparar la percepción de imagen corporal de jóvenes normopeso y con sobrepeso/obesidad. MATERIAL Y MÉTODOS: se comparó a 376 jóvenes de ambos sexos, de una muestra general y sin tratamiento, de entre 18 y 25 años de edad, clasificados por su índice de masa corporal en dos grupos: grupo normopeso y grupo sobrepeso u obesidad. Para evaluar imagen corporal, los participantes respondieron el Multidimensional Body Self Relations Questionnaire (MBSRQ) y el Eating Disorders Inventory (EDI-3). RESULTADOS: los participantes con sobrepeso/obesidad están menos satisfechos con su apariencia y con su cuerpo cuando este es evaluado por partes, evalúan peor su estado físico y/o sus capacidades físicas, presentan menos conductas orientadas a mantener o mejorar su forma física, perciben un peor diagnóstico de su salud y tienen menor satisfacción corporal. Asimismo, están más preocupados por su peso, se autoclasifican más por su peso y presentan una mayor obsesión por la delgadez. Además, las mujeres, en comparación con los hombres, presentan una evaluación más negativa de su imagen corporal. CONCLUSIÓN: a pesar de la mala evaluación que los estudiantes con sobrepeso/obesidad realizan en relación a su cuerpo, no están dispuestos a realizar actividades concretas que les permitan cambiar su estado. Es necesario seguir investigando con el fin de desarrollar programas preventivos y de tratamiento más efectivos.

Contextos habituales de alimentación en edad temprana: estudio en díadas cuidador principal e hijo o hija / Usual feeding context in early childhood. Study of the caregiver-.baby dyed and the alimentary style of the main caregiver

Es importante investigar el inicio de la conducta alimentaria dadas las altas tasas de obesidad infantil a nivel mundial y en Chile. Diversas investigaciones dan importancia al rol que cumplen los padres en la formacion de hábitos alimentarios durante la primera infancia. Este trabajo pretende relacionar las características del contexto alimentario cotidiano en infancia temprana con la emocionalidad de la díada cuidador-bebé, y estilo alimentario del cuidador principal. Estudio descriptivo, correlacional y transversal. Observación por jueces expertos de 29 videograbaciones de díadas cuadador-bebé en una práctica alimentaria habitual. La media de edad de los/las niños/as fue de 2,12 años (DS= 0,48), la mayoría presentan un estado nutricional normal (51,7%), y el 62% eran varones. Cuestionarios aplicados: encuesta sociodemográfica, lista de chequeo de conductas alimentarias habituales, medición de estado socioemocional del niño (CBCL y ASQ-SE), sintomatología afectiva y alimentaria del cuidador principal (DASS 21 y DEBQ ). Las conductas alimentarias problemáticas más frecuentes de los niños son: rechazar comer alimentos específicos por textura/sabor y un estado emocional tenso durante la alimentación. Los síntomas depresivos y de estrés del cuidador se asociaron significativamente con el comportamiento del menor durante la práctica alimentaria cotidiana. Se observa una relación significativa entre la percepción de los estados emocionales del niño por parte de su cuidador, un contexto no habitual para realizar la práctica alimentaria y la actitud más directiva que asume el adulto durante la interacción alimentaria. Es necesario investigar la interacción alimentaria especialmente durante los primeros años de vida, momento en que se establecen los hábitos alimentarios.

It is important to investigate the beginning of eating behavior given the high rates of child obesity that exist all around the world and in Chile. A large number of investigations give importance to the role of parents in the formation of eating habits during early childhood. This work aims to relate the characteristics of the usual feeding context in early childhood with the emotionality of the caregiverbaby dyad, and the alimentary style of the main caregiver. Descriptive, correlational and transversal study. Observation by expert judges of 29 video recordings of caregiver-baby dyad in a habitual feeding practice. The average age of the children was 2.12 years (DS = 0.48), most of them had normal nutritional status (51.7%), and 62% were males. Applied questionnaires: socio-demographic survey, checklist of habitual eating behaviors, measurement of the child's socio-emotional state (CBCL Y ASQ-SE), affective and alimentary symptomatology of the main caregiver (DASS 21 Y DEBQ ). The most frequent problematic eating behaviors of children are: Reject to eat specific foods due to texture/flavour and a tense emotional state during the feeding. The depressive and stress symptoms of the caregiver are significantly associated with the behavior of the child during the habitual feeding practice. It is noted a significant relationship between the caregiver's perception of the child's emotional state, an unusual context to perform feeding practice and the more directive attitude assumed by the adult during the alimentary interaction. It is necessary to investigate the food interaction especially during the first years of life, when eating habits are established.

Copro-prevalence of Fasciola hepatica in Chilean breed horses in the province of Concepción, Chile / Copro-prevalência de Fasciola hepatica em equinos de raça chilena na província de Concepción, Chile

Abstract The aim of this research was to assess the copro-prevalence of Fasciola hepatica in owned Chilean breed horses (Equus caballus) residing in the province of Concepción, Chile. The study was carried out throughout October 2017. Samples were taken from all (100%) Chilean breed horse (124 specimens; 45 females and 79 males, aged between 7 months and 24 years old) from the Concepción province. A rectal stool sample was obtained from each animal. This was analyzed using the Army Medical School method (AMS III) technique to detect F. hepatica eggs. Information about the horse's age, sex, previous fasciolicide application, and an assessment of body condition was also obtained. Fisher tests were carried out to analyze the results. Ten horses (8.06%) were positive for the presence of F. hepatica eggs. No significant difference of copro-prevalence was found between age (young 8.47%, old 7.69%), sex (female 11.1%, male 6.33%), the previous application of fasciolicide (dewormed 10%, not dewormed 7.89%), or body condition (low condition 11.1%, high condition 0.7%) categories (Fisher tests: P>0.05 in all cases). The results of this study suggest that Chilean breed horses constitute part of the reservoir of F. hepatica for both ruminant populations and humans in the Concepción province.

Resumo O objetivo desta pesquisa foi avaliar a coproprevalência de Fasciola hepatica em equinos chilenos (Equus caballus), residentes na província de Concepción, região de Bío-Bío, Chile. O estudo foi realizado durante o mês de outubro de 2017. Foram coletadas amostras de todos os equinos estabulados (124 espécimes; 45 fêmeas e 79 machos, com idades entre 7 meses e 24 anos) da província de Concepción. Uma amostra de fezes transretal foi obtida de cada animal, analisada no Laboratório "Dr. Luis Rubilar", da Universidad de Concepción, usando-se a técnica AMS III, para detectar a presença de ovos de F. hepatica. O diagnóstico fecal foi complementado com informações sobre a idade do animal, sexo, aplicação prévia de fasciolicida e uma avaliação da condição corporal. Testes de Fisher foram realizados para analisar os resultados. Dos 124 equinos, 10 (8,06%) foram positivos para ovos de F. hepatica. Não foi encontrada associação significativa entre idade, sexo, aplicação prévia de fasciolicida ou condição corporal com a presença de ovos. Um cavalo tratado com triclabendazol foi positivo para ovos de F. hepatica.