Ciprofloxacin and cefazolin as a combination for empirical initial therapy of peritoneal dialysis-related peritonitis: five-year follow-up.

The treatment of peritoneal dialysis (PD)-related peritonitis has been a matter of extensive investigation, frequently generating therapeutic trials. Several combinations of antibiotics have served as newer protocols and tended to be efficacious, comfortable, and cost-effective. According to the more recent recommendations from the International Society for Peritoneal Dialysis, the rationale for empirical initial therapy of clinically detected peritonitis in PD patients has been to follow the bacterial profile derived from cultured specimens of PD effluents. The current study describes 5 year's experience with the use of a new antibiotic regimen for the treatment of peritonitis. We herein analyze the outcome of 95 episodes of peritonitis in 54 patients on either automated PD or continuous ambulatory PD at the dialysis unit of the Federal University of Rio de Janeiro. Peritoneal dialysis-related peritonitis was treated with the combination of oral ciprofloxacin and intraperitoneal cefazolin. The observed cure rate was 85.2% and the sensitivity test was observed to be positive for this combination of antibiotics in 88.9% of positive cultures. Of the 14 unsuccessful episodes, 7 were due to catheter colonization and the rest did not respond to the proposed therapy within 48 hours. These 7 cases were also related to peritoneal fluid cultures that were resistant to both ciprofloxacin and cefazolin. From this study, we propose this combination of oral ciprofloxacin and intraperitoneal cefazolin as a first choice for empirical initial therapy of PD-related peritonitis, given its efficacy and low cost. However, in order to apply the most adequate cost-effective therapy, careful examination of the bacterial profile and sensitivities to antibiotics used in each unit is strongly recommended.

Variability of carbon and water fluxes following climate extremes over a tropical forest in southwestern Amazonia.

The carbon and water cycles for a southwestern Amazonian forest site were investigated using the longest time series of fluxes of CO2 and water vapor ever reported for this site. The period from 2004 to 2010 included two severe droughts (2005 and 2010) and a flooding year (2009). The effects of such climate extremes were detected in annual sums of fluxes as well as in other components of the carbon and water cycles, such as gross primary production and water use efficiency. Gap-filling and flux-partitioning were applied in order to fill gaps due to missing data, and errors analysis made it possible to infer the uncertainty on the carbon balance. Overall, the site was found to have a net carbon uptake of ≈5 t C ha(-1) year(-1), but the effects of the drought of 2005 were still noticed in 2006, when the climate disturbance caused the site to become a net source of carbon to the atmosphere. Different regions of the Amazon forest might respond differently to climate extremes due to differences in dry season length, annual precipitation, species compositions, albedo and soil type. Longer time series of fluxes measured over several locations are required to better characterize the effects of climate anomalies on the carbon and water balances for the whole Amazon region. Such valuable datasets can also be used to calibrate biogeochemical models and infer on future scenarios of the Amazon forest carbon balance under the influence of climate change.

The development and psychometric evaluation of a self-efficacy scale for practicing pelvic floor exercises.

BACKGROUND: Self-efficacy has been shown to be a predictor of many health-related behaviors, including the practice of pelvic floor exercises with a focus on prevention or cure. OBJECTIVES: To describe the process of construction and the psychometric properties of the scale of self-efficacy for the practice of pelvic floor exercises (EAPEAP). METHOD: A cross-sectional study of validation was carried out with 81 from community and 96 postpartum women, 54.8% of them complained of urinary leakage. An exploratory factor analysis and internal consistency analysis was performed. To check predictive capacity, we analyzed the adherence at 3 months post - intervention and compared the scores of self-efficacy between adherent and non-adherent women. Reliability was analyzed by split half procedure. RESULTS: The instrument showed α=0.923, and revealed three factors: performance expectation considering the action, performance expectation considering the preparation for action and outcome expectations. These factors accounted for 65.32% of the total variance. The instrument was able to differentiate between women who adhere and have not adhered to the exercises (U=352, p=0.013) and there was strong correlation between the two halves of the instrument (rho=0.889, p<0.001). CONCLUSION: The scale is a valid and reliable tool to measure self-efficacy to practice pelvic floor exercises.

"Ten Commandments" for the Appropriate use of Antibiotics by the Practicing Physician in an Outpatient Setting.

A multi-national working group on antibiotic stewardship, from the International Society of Chemotherapy, put together ten recommendations to physicians prescribing antibiotics to outpatients. These recommendations are: (1) use antibiotics only when needed; teach the patient how to manage symptoms of non-bacterial infections; (2) select the adequate ATB; precise targeting is better than shotgun therapy; (3) consider pharmacokinetics and pharmacodynamics when selecting an ATB; use the shortest ATB course that has proven clinical efficacy; (4) encourage patients' compliance; (5) use antibiotic combinations only in specific situations; (6) avoid low quality and sub-standard drugs; prevent prescription changes at the drugstore; (7) discourage self-prescription; (8) follow only evidence-based guidelines; beware those sponsored by drug companies; (9) rely (rationally) upon the clinical microbiology lab; and (10) prescribe ATB empirically - but intelligently; know local susceptibility trends, and also surveillance limitations.

The development and psychometric evaluation of a self-efficacy scale for practicing pelvic floor exercises / Construcao e avaliacao psicometrica da escala de autoeficacia para pratica de exercicios do assoalho pelvico

BACKGROUND: Self-efficacy has been shown to be a predictor of many health-related behaviors, including the practice of pelvic floor exercises with a focus on prevention or cure. OBJECTIVES: To describe the process of construction and the psychometric properties of the scale of self-efficacy for the practice of pelvic floor exercises (EAPEAP). METHOD: A cross-sectional study of validation was carried out with 81 from community and 96 postpartum women, 54.8% of them complained of urinary leakage. An exploratory factor analysis and internal consistency analysis was performed. To check predictive capacity, we analyzed the adherence at 3 months post - intervention and compared the scores of self-efficacy between adherent and non-adherent women. Reliability was analyzed by split half procedure. RESULTS: The instrument showed α=0.923, and revealed three factors: performance expectation considering the action, performance expectation considering the preparation for action and outcome expectations. These factors accounted for 65.32% of the total variance. The instrument was able to differentiate between women who adhere and have not adhered to the exercises (U=352, p=0.013) and there was strong correlation between the two halves of the instrument (rho=0.889, p<0.001). CONCLUSION: The scale is a valid and reliable tool to measure self-efficacy to practice pelvic floor exercises. .

CONTEXTUALIZAÇÃO: A autoeficácia tem se mostrado preditora de muitos comportamentos relacionados à saúde, dentre eles, a prática de exercícios do assoalho pélvico (AP) com foco na prevenção ou cura. OBJETIVO: Descrever o processo de construção e as propriedades psicométricas da Escala de Autoeficácia para Prática de Exercícios do Assoalho Pélvico (EAPEAP). MÉTODO: Estudo transversal de validação com 81 mulheres da comunidade e 96 puérperas, sendo que 54,8% queixavam-se de perdas urinárias. Procedeu-se com análise fatorial exploratória e análise de consistência interna. Para verificar capacidade preditiva, analisou-se a aderência em três meses pós- intervenção e comparou-se o escore de autoeficácia entre mulheres aderentes e não aderentes. A fidedignidade foi avaliada pela técnica das metades partidas. RESULTADOS: O instrumento obteve α=0,923 e revelou três fatores: expectativa de performance considerando a ação, expectativa de performance considerando a preparação para a ação e expectativa de resultado. Tais fatores foram responsáveis por 65,32% da variância total. O instrumento foi capaz de diferenciar as mulheres que aderiram daquelas que não aderiram aos exercícios (U=352, p=0,013), e houve correlação forte entre as duas metades do instrumento (rho=0,889, p<0,001). CONCLUSÃO: A escala é válida e confiável para mensurar autoeficácia para prática de exercícios do assoalho pélvico. .

Cultural universals and differences in male homosexuality: the case of a Brazilian fishing village.

This study investigated whether homosexually identified males in a small Brazilian fishing community are similar to homosexually identified males described in the international literature. In addition, it attempted to distinguish heterosexuals who have sex with homosexuals from other heterosexuals. A total of 41 men were classified into three sexual categories: paneleiros ("pan cabinets"), who have sex only with men, and invariably so in a bottom position; persistent heterosexuals, who have sex only with women; and paneleiro lovers, who have sex with women and with paneleiros, but invariably in a top position. There were significant differences in childhood play activities, sexual inclinations, and sports preferences between paneleiros and non-paneleiros; however, paneleiro lovers and persistent heterosexuals were not significantly different with regard to these variables or with regard to access to women. These findings add to the cross-cultural literature on childhood cross-gender behavior and homoerotic activities among non-homosexually identified males.

Força dos músculos do assoalho pélvico e função sexual em gestantes / Strength of pelvic floor muscles and sexual function during pregnancy

Contextualização: O bem-estar sexual depende de músculos do assoalho pélvico (MAP) fortes o suficiente para manter a sua função. Durante a gestação, tanto a função sexual como a força dos MAP podem modificar-se. OBJETIVOS: Comparar o grau de força dos MAP e a função sexual em gestantes do segundo e terceiro trimestres. MÉTODOS: Pesquisa descritiva causal comparativa realizada com 37 gestantes de Florianópolis (18 do segundo e 19 do terceiro trimestre), com média de idade de 25,22 anos (±5,7 anos). Os instrumentos utilizados foram o Questionário Female Sexual Function Index (FSFI) e o Teste Manual da Musculatura do Assoalho Pélvico, utilizando a escala de Oxford modificada para graduação da força. Os dados foram analisados por meio de estatística descritiva e inferencial (teste t independente, teste U de Mann Whitney, correlação de Spearman), nível de significância de 0,05. RESULTADOS: Não houve diferença significativa entre a média dos valores dos postos do grau de contração dos MAP de gestantes do segundo e do terceiro trimestre (U=150,5; p=0,512). Todavia, a função sexual das gestantes do segundo trimestre de gestação foi melhor que as do terceiro (U=104; p=0,042), e o grau de contração dos MAP apresentou correlações estatisticamente significativas com a idade (ρ=0,320, p=0,041) e com o escore do FSFI (ρ=0,540, p<0,001). CONCLUSÕES: A função sexual diminuiu significativamente do segundo para o terceiro trimestre, enquanto que a força dos MAP não apresentou diferença entre os trimestres.

Background: Sexual well-being depends on pelvic floor muscles (PFMs) that are strong enough to maintain their function. During pregnancy, both the sexual function and the strength of the PFMs may be altered. OBJECTIVES: to compare the degree of PFM strength and the sexual function of pregnant women in the second and the third trimesters. METHODS: a descriptive, causal-comparative study was carried out with 37 pregnant women in Florianópolis (18 in the second trimester and 19 in the third trimester) with a mean age of 25.22 years (±5.7 years). The instruments used were the Female Sexual Function Index (FSFI) Questionnaire and the Manual Test of Pelvic Floor Muscle Strength, using the modified Oxford scale to grade strength. The data were analyzed using descriptive and inferential statistics (independent t test, the Mann-Whitney U test, Spearman's correlation) with a significance level of 0.05. RESULTS: There was no significant difference between the mean rank values of PFM strength of pregnant women in the second and third trimester (U=150.5; p=0.512). However, the sexual function of the pregnant women in the second trimester of pregnancy was better than that of the women in the third trimester (U=104; p=0.042). In addition, PFM strength had statistically significant correlations with age (ρ=0.320, p=0.041) and with FSFI score (ρ=0.540, p<0.001). CONCLUSIONS: Sexual function decreased significantly from the second to the third trimester while PFM strength did not differ between trimesters.

Whole blood assay to access T cell-immune responses to Mycobacterium tuberculosis antigens in healthy Brazilian individuals.

The production of interferon gamma (IFNgamma) guarantees effective T cell-mediated immunity against Mycobacterium tuberculosis infection. In the present study, we simply compare the in vitro immune responses to Mycobacterium antigens in terms of IFNg production in a total of 10 healthy Brazilian volunteers. Whole blood and mononuclear cells were cultivated in parallel with PPD, Ag85B, and M. bovis hsp65, and five-days supernatants were harvested for cytokine detection by ELISA. The inter-assay result was that the overall profile of agreement in response to antigens was highly correlated (r(2) = 0.9266; p = 0.0102). Potential analysis is in current progress to dictate the usefulness of this method to access the immune responses also in tuberculosis patients and its contacts.

T-cell responses to the Mycobacterium tuberculosis-specific antigen ESAT-6 in Brazilian tuberculosis patients.

The Mycobacterium tuberculosis-specific ESAT-6 antigen induces highly potent T-cell responses and production of gamma interferon (IFN-gamma), which play a critical role in protective cell-mediated immunity against tuberculosis (TB). In the present study, IFN-gamma secretion by peripheral blood mononuclear cells (PBMCs) in response to M. tuberculosis ESAT-6 in Brazilian TB patients was investigated in relation to clinical disease types, such as pleurisy and cavitary pulmonary TB. Leprosy patients, patients with pulmonary diseases other than TB, and healthy donors were assayed as control groups. Sixty percent of the TB patients indeed recognized M. tuberculosis ESAT-6, as did 50% of the leprosy patients and 60% of the non-TB controls. Nevertheless, the levels of IFN-gamma in response to the antigen ESAT, but not to antigen 85B (Ag85B) and purified protein derivative (PPD), were significantly lower in controls than in patients with treated TB or pleural or cavitary TB. Moreover, according to Mycobacterium bovis BCG vaccination status, only 59% of the vaccinated TB patients responded to ESAT in vitro, whereas 100% of them responded to PPD. Both CD4 and CD8 T cells were able to release IFN-gamma in response to ESAT. The present data demonstrate the specificity of ESAT-6 of M. tuberculosis and its ability to discriminate TB patients from controls, including leprosy patients. However, to obtain specificity, it is necessary to include quantitative IFN-gamma production in response to the antigen as well, and this might limit the use of ESAT-6-based immunodiagnosis of M. tuberculosis infection in an area of TB endemicity.

Whole blood assay to access T cell-immune responses to Mycobacterium tuberculosis antigens in healthy Brazilian individuals

The production of interferon gamma (IFNgamma) guarantees effective T cell-mediated immunity against Mycobacterium tuberculosis infection. In the present study, we simply compare the in vitro immune responses to Mycobacterium antigens in terms of IFNg production in a total of 10 healthy Brazilian volunteers. Whole blood and mononuclear cells were cultivated in parallel with PPD, Ag85B, and M. bovis hsp65, and five-days supernatants were harvested for cytokine detection by ELISA. The inter-assay result was that the overall profile of agreement in response to antigens was highly correlated (r² = 0.9266; p = 0.0102). Potential analysis is in current progress to dictate the usefulness of this method to access the immune responses also in tuberculosis patients and its contacts.

T-cell responses to the Mycobacterium tuberculosis-specific antigen ESAT-6 in Brazilian tuberculosis patients

The Mycobacterium tuberculosis-specific ESAT-6 antigen induces highly potent T-cell responses and production of gamma interferon (IFN-gamma), which play a critical role in protective cell-mediated immunity against tuberculosis (TB). In the present study, IFN-gamma secretion by peripheral blood mononuclear cells (PBMCs) in response to M. tuberculosis ESAT-6 in Brazilian TB patients was investigated in relation to clinical disease types, such as pleurisy and cavitary pulmonary TB. Leprosy patients, patients with pulmonary diseases other than TB, and healthy donors were assayed as control groups. Sixty percent of the TB patients indeed recognized M. tuberculosis ESAT-6, as did 50 per cent of the leprosy patients and 60 per cent of the non-TB controls. Nevertheless, the levels of IFN-gamma in response to the antigen ESAT, but not to antigen 85B (Ag85B) and purified protein derivative (PPD), were significantly lower in controls than in patients with treated TB or pleural or cavitary TB. Moreover, according to Mycobacterium bovis BCG vaccination status, only 59 per cent of the vaccinated TB patients responded to ESAT in vitro, whereas 100 per cent of them responded to PPD. Both CD4 and CD8 T cells were able to release IFN-gamma in response to ESAT. The present data demonstrate the specificity of ESAT-6 of M. tuberculosis and its ability to discriminate TB patients from controls, including leprosy patients. However, to obtain specificity, it is necessary to include quantitative IFN-gamma production in response to the antigen as well, and this might limit the use of ESAT-6-based immunodiagnosis of M. tuberculosis infection in an area of TB endemicity.