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1.
Ergonomics ; 67(1): 111-122, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-37083559

RESUMO

This study aimed to identify the most important, frequently performed, and physically demanding tasks performed by Brazilian firefighters and to identify tasks that could be used to assess physical fitness. A subjective task analysis was conducted. Five hundred twenty-four firefighters (84% male; 16% females) responded to an online survey and rated 37 tasks across three domains (most important, most frequent, and most physically demanding). A dichotomous decision analysis was used to inform the proposed physical fitness tests. Wildland firefighting tasks presented the highest overall mean rate. Traffic control was considered the most important and frequently performed task. Lifeguard rescue was considered the most physically demanding task. The dichotomous analysis identified 14 essential tasks (seven structural firefighting and seven automobile accidents). The tasks identified may be helpful in developing criterion physical fitness tests and training programs related to firefighters' demands.Practitioner summary: The unpredictability, variability, and dangerousness of firefighting make it challenging to observe the physical demands imposed on firefighters. A subjective task analysis was conducted to identify essential tasks performed by Brazilian firefighters. Wildland firefighting, lifeguard rescue, automobile accidents, and structural firefighting tasks were the most important, frequent, and physically demanding.


Assuntos
Bombeiros , Feminino , Humanos , Masculino , Brasil , Análise e Desempenho de Tarefas , Aptidão Física , Exercício Físico
2.
Vet Surg ; 50 Suppl 1: O128-O137, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33280152

RESUMO

OBJECTIVE: To report the diagnostics, surgical treatment, and outcome of a juvenile foal with solitary osteochondroma (SO) interfering with the podotrochlear apparatus. STUDY DESIGN: Case report ANIMAL: One 36-day-old Arabian colt. METHODS: Clinical, radiographic, ultrasonographic, computed tomographic, and histopathologic examinations were required to characterize and treat an SO located at the palmar aspect of the diaphysis of the second phalanx of the left forelimb. This SO caused severe distal interphalangeal joint (DIPJ) inflammation, marked interference with the podotrochlear apparatus, and associated lameness. RESULTS: Despite the small size of the foal's foot, complete resection of the SO was possible via palmar DIPJ arthroscopy by using motorized equipment. Full resolution of the lameness was achieved within 3 months of surgery. CONCLUSION: Atypical SO located on the palmar aspect of the second phalanx can cause marked nonseptic inflammation of the DIPJ and interference with the podotrochlear apparatus and should be considered among the differential diagnoses for severe lameness in juvenile foals. Arthroscopic resection of the SO led to an excellent outcome.


Assuntos
Doenças dos Cavalos , Osteocondroma , Animais , Artroscopia/veterinária , Membro Anterior , Doenças dos Cavalos/cirurgia , Cavalos , Articulações/cirurgia , Masculino , Osteocondroma/cirurgia , Osteocondroma/veterinária
3.
Mol Biotechnol ; 66(2): 354-364, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-37162721

RESUMO

This work aimed to assess the Sf9 cell metabolism during growth, and infection steps with recombinant baculovirus bearing rabies virus proteins, to finally obtain rabies VLP in two culture systems: Schott flask (SF) and stirred tank reactor (STR). Eight assays were performed in SF and STR (four assays in each system) using serum-free SF900 III culture medium. Two non-infection growth kinetics assays and six recombinant baculovirus infection assays. The infection runs were carried out at 0.1 pfu/cell multiplicity of infection (MOI) for single baculovirus bearing rabies glycoprotein (BVG) and matrix protein (BVM) and a coinfection with both baculoviruses at MOI of 3 and 2 pfu/cell for BVG and BVM, respectively. The SF assays were done in triplicate. The glucose, glutamine, glutamate, lactate, and ammonium uptake or release specific rates were quantified over the exponential growth phase and infection stage. The highest uptake specific rate was observed for glucose (42.5 × 10-12 mmol cell/h) in SF and for glutamine (30.8 × 10-12 mmol/cell/h) in STR, in the exponential growth phases. A wave pattern was observed for assessed analytes throughout the infection phase and the glucose had the highest wave amplitude within the 10-10 mmol cell/h order. This alternative uptake and release behavior is in harmony with the lytic cycle of baculovirus in insect cells. The virus propagation and VLP generation were not limited by glucose, glutamine, and glutamate, neither by the toxicity of lactate nor ammonium under the conditions appraised in this work. The findings from this work can be useful to set baculovirus infection processes at high cell density to improve rabies VLP yield, purity, and productivity.


Assuntos
Compostos de Amônio , Vírus da Raiva , Raiva , Animais , Células Sf9 , Vírus da Raiva/genética , Glutamina , Baculoviridae/genética , Proteínas Recombinantes/genética , Meios de Cultura Livres de Soro , Ácido Glutâmico , Lactatos , Glucose , Spodoptera
4.
Vet Med Sci ; 9(4): 1599-1609, 2023 07.
Artigo em Inglês | MEDLINE | ID: mdl-37221932

RESUMO

BACKGROUND: Upper respiratory disease was reported over many seasons in Arabian foals on a single stud farm in the Middle East. Affected foals were noted to have mucopurulent nasal discharge, cough, fever and tachypnea. All affected foals had been empirically treated with a macrolide and rifampicin, by the referring veterinarian without improvement. On endoscopic examination, all affected foals had significant guttural pouch empyema (GPE). OBJECTIVES: (1) To document a previously unreported presentation of guttural pouch empyema (GPE) in a family of juvenile Arabian foals; (2) To document the cytological and microbial composition of the empyema; (3) To identify clinical signs significantly correlated with the presence of GPE, as predictors for the need for guttural pouch (GP) endoscopy; (4) To demonstrate successful resolution of the identified syndrome with mechanical GP lavage and evidence based antimicrobial use, improving antibiotic stewardship and the one-health approach to respiratory disease in this demographic of foals. METHODS: Evaluation and scoring of clinical signs, upper airway endoscopy and thoracic ultrasound were performed in 14 affected foals and 10 age-matched controls, followed by comparative tracheal and guttural pouch sputum culture and cytological evaluation. Therapeutic GP lavage was performed and response to therapy monitored. RESULTS: GPE, cranioventrally distributed ultrasonographic lesions and opportunistic pathogen infection suggested a primary lesion of GPE with aspiration of GP discharge into the lungs. GP lavage resolved the empyema and associated clinical signs in all cases. CONCLUSIONS: Cytological examination of tracheal and guttural pouch aspirates revealed a neutrophilic exudate with lipid-laden phagocytes, suggestive of engulfed milk. Bacteriology revealed a high prevalence of Streptococcus equi ssp. zooepidemicus admixed with other opportunistic pathogens. Streptococcus equi ssp. equi was not isolated in any case.


Assuntos
Empiema , Doenças dos Cavalos , Streptococcus equi , Animais , Cavalos , Estudos de Casos e Controles , Doenças dos Cavalos/patologia , Endoscopia/veterinária , Empiema/veterinária
5.
J Biotechnol ; 363: 19-31, 2023 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-36587847

RESUMO

This work aimed to quantify growth and biochemical parameters (viable cell density, Xv; cell viability, CV; glucose, lactate, glutamine, glutamate, ammonium, and potassium concentrations) in upstream stages to obtain rabies virus-like particles (rabies VLP) from insect cell-baculovirus system using on-line and off-line Raman spectra to calibrate global models with minimal experimental data. Five cultivations in bioreactor were performed. The first one comprised the growth of uninfected Spodoptera frugiperda (Sf9) cells, the second and third runs to obtain recombinant baculovirus (rBV) bearing Rabies G glycoprotein and matrix protein, respectively. The fourth one involved the generation of rabies VLP from rBVs and the last one was a repetition of the third one with cell inoculum infected by rBV. The spectra were acquired through a Raman spectrometer with a 785-nm laser source. The fitted Partial Least Square models for nutrients and metabolites were comparable with those previously reported for mammalian cell lines (Relative error < 15 %). However, the use of this chemometrics approach for Xv and CV was not as accurate as it was for other parameters. The findings from this work established the basis for bioprocess Raman spectroscopical monitoring using insect cells for VLP manufacturing, which are gaining ground in the pharmaceutical industry.


Assuntos
Vírus da Raiva , Raiva , Animais , Vírus da Raiva/genética , Análise Espectral Raman , Linhagem Celular , Reatores Biológicos , Baculoviridae , Proteínas Recombinantes , Insetos , Spodoptera , Mamíferos
6.
J Equine Vet Sci ; 108: 103795, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34800799

RESUMO

Equine infectious anemia (EIA) is listed by the World Organization for Animal Health (OIE) as one of the equine diseases that must be notified. No effective treatment or vaccine is available. EIA control is based on segregation and euthanasia of positive equids. The disease is caused by the equine infectious anemia virus (EIAV), a member of the genus Lentivirus of the Retroviridae family. Despite the importance of this disease in equids, EIA has been poorly studied in donkeys (Equus asinus). We evaluate the sanitary conditions related to EIAV in donkeys from a shelter of abandoned animals captured on the roads of the Ceará. A total of 124 donkeys were randomly selected, and three horses lived at the same shelter. The animals were clinically evaluated, and a group of the 20 animals was submitted to hematological tests. Three diagnostic tests for EIA were used, agar gel immunodiffusion (AGID), enzyme-linked immunosorbent assay (ELISA) using EIAV recombinant protein gp90 (rgp90) and recombinant protein p26 (rp26) ELISA, and polymerase chain reaction (PCR) for detection of the EIAV tat-gag gene. From the donkeys, only 1 animal was positive using AGID 0.81% (1/124), compared to 21.8% (27/124) in the rgp90 and 10.5% (13/124) in the rp26 ELISA. Proviral DNA was detected by PCR tat-gag in 8.8% (11/124), and phylogenetic analysis confirms that the EIAV sequences of donkeys from the Brazilian Northeast grouped with Pantanal Brazilian sequences. Thus, in light of the results, we conclude that donkeys are carriers of EIAV and could be sources of infection.


Assuntos
Anemia Infecciosa Equina , Vírus da Anemia Infecciosa Equina , Animais , Equidae , Anemia Infecciosa Equina/diagnóstico , Eutanásia Animal , Cavalos , Vírus da Anemia Infecciosa Equina/genética , Filogenia
7.
Vaccines (Basel) ; 11(1)2022 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-36679884

RESUMO

This work aimed to assess, following upstream optimization in Schott flasks, the scalability from this culture platform to a stirred-tank bioreactor in order to yield rabies-recombinant baculovirus, bearing genes of G (BVG) and M (BVM) proteins, and to obtain rabies virus-like particles (VLP) from them, using Sf9 insect cells as a host. Equivalent assays in Schott flasks and a bioreactor were performed to compare both systems and a multivariate statistical approach was also carried out to maximize VLP production as a function of BVG and BVM's multiplicity of infection (MOI) and harvest time (HT). Viable cell density, cell viability, virus titer, BVG and BVM quantification by dot-blot, and BVG quantification by Enzyme-Linked Immunosorbent Assay (ELISA) were monitored throughout the assays. Furthermore, transmission electron microscopy was used to characterize rabies VLP. The optimal combination for maximum VLP expression was BVG and BVM MOI of 2.3 pfu/cell and 5.1 pfu/cell, respectively, and 108 h of harvest time. The current study confirmed that the utilization of Schott flasks and a benchtop bioreactor under the conditions applied herein are equivalent regarding the cell death kinetics corresponding to the recombinant baculovirus infection process in Sf9 cells. According to the results, the hydrodynamic and chemical differences in both systems seem to greatly affect the virus and VLP integrity after release.

8.
J Biotechnol, v. 363, p. 19-31, fev. 2023
Artigo em Inglês | SES-SP, SESSP-IBPROD, SES-SP | ID: bud-4763

RESUMO

This work aimed to quantify growth and biochemical parameters (viable cell density, Xv; cell viability, CV; glucose, lactate, glutamine, glutamate, ammonium, and potassium concentrations) in upstream stages to obtain rabies virus-like particles (rabies VLP) from insect cell-baculovirus system using on-line and off-line Raman spectra to calibrate global models with minimal experimental data. Five cultivations in bioreactor were performed. The first one comprised the growth of uninfected Spodoptera frugiperda (Sf9) cells, the second and third runs to obtain recombinant baculovirus (rBV) bearing Rabies G glycoprotein and matrix protein, respectively. The fourth one involved the generation of rabies VLP from rBVs and the last one was a repetition of the third one with cell inoculum infected by rBV. The spectra were acquired through a Raman spectrometer with a 785-nm laser source. The fitted Partial Least Square models for nutrients and metabolites were comparable with those previously reported for mammalian cell lines (Relative error < 15 %). However, the use of this chemometrics approach for Xv and CV was not as accurate as it was for other parameters. The findings from this work established the basis for bioprocess Raman spectroscopical monitoring using insect cells for VLP manufacturing, which are gaining ground in the pharmaceutical industry.

9.
Ciênc. anim. bras. (Impr.) ; 24: 74483, 2023. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1439864

RESUMO

The objective of this work was to evaluate several attempts to knock down neats and to correlate the success obtained with actions performed by the horse/rider sets through an analysis of public domain videos of vaquejadas. Approximately 300 videos were considered, and the groups were evaluated according to the competition category, that is, amateur/aspirant and professional, and the animals used in the test, that is, pulling or tracking of the neat. It was found that for the pull set in the professional competition category, only the characteristics of directing the cow on the track, and the guiding behavior and positioning in the pull were significant (p<0.01), while for the amateur/aspirant category, the characteristics of remaining in the pull after falling of the cattle and of the behavior at the gate had an effect (p<0.01). The characteristic of remaining in the pull after the fall of the neats, in the category of professional pulling athletes, exists in greater prevalence during the competition with 85.3%, while the characteristic of directing the neat to the side of the track is performed by 87.6% of the competitors. For the set used to track the cattle, the values of the dimensions 1 and 2 were 96.56% and 3.43%, respectively, which is 99.9% of the total variance of the data based on those selected for the final result of "earn the ox". The use of multiple diversity analysis in this study of variables associated with the vaquejada set proved to be quite advantageous, and there was not much variation in the elements obtained with a greater weight in the determination of the final result.(AU)


Objetivou-se com este trabalho avaliar por meio de análise de vídeos de domínio público de competições de vaquejadas, diversas tentativas de derrubada de rezes e correlacionar o êxito obtido com ações desempenhadas pelos conjuntos cavalo/ cavaleiro. Foram considerados aproximadamente 300 vídeos, sendo os grupos avaliados quanto à categoria de competição, Amador/Aspirante e Profissional, e aos animais utilizados na prova, Puxar ou Esteirar a rês. Verificou-se que para o conjunto de puxar, da categoria profissional de competição, apenas as características de direcionamento da rês na pista, comportamento na condução e posicionamento na puxada foram significativos (p<0,01), enquanto para a categoria amador/ aspirante houve efeito (p<0,01) para a característica de permanecer ou não na puxada após queda da rês e para o comportamento na porteira. A característica, permanecer na puxada após a queda da rês, na categoria de atletas puxadores profissionais, existe em maior prevalência em ações durante a prova com 85,3% dos casos, enquanto a característica de direcionar a rês para a lateral da pista é realizada por 87,6% dos competidores. Para o conjunto utilizado para esteirar a rês, os valores das dimensões 1 e 2 foram de 96,56% e 3,43%, respectivamente, totalizando 99,9% da variância total dos dados com base nos critérios selecionados para o resultado final de "Valeu o boi". O uso da análise de correspondência múltipla no estudo de variáveis associadas ao sucesso em competições de vaquejada mostrou-se bastante eficaz, no que tange a diminuição do universo de elementos com maior peso de determinação no resultado final obtido.(AU)


Assuntos
Animais , Atletismo/fisiologia , Esforço Físico/fisiologia , Cavalos , Comportamento Animal
10.
Mol Biotechnol, v. 66, p. 354-364, abr. 2023
Artigo em Inglês | SES-SP, SESSP-IBPROD, SES-SP | ID: bud-4915

RESUMO

This work aimed to assess the Sf9 cell metabolism during growth, and infection steps with recombinant baculovirus bearing rabies virus proteins, to fnally obtain rabies VLP in two culture systems: Schott fask (SF) and stirred tank reactor (STR). Eight assays were performed in SF and STR (four assays in each system) using serum-free SF900 III culture medium. Two non-infection growth kinetics assays and six recombinant baculovirus infection assays. The infection runs were carried out at 0.1 pfu/cell multiplicity of infection (MOI) for single baculovirus bearing rabies glycoprotein (BVG) and matrix protein (BVM) and a coinfection with both baculoviruses at MOI of 3 and 2 pfu/cell for BVG and BVM, respectively. The SF assays were done in triplicate. The glucose, glutamine, glutamate, lactate, and ammonium uptake or release specifc rates were quantifed over the exponential growth phase and infection stage. The highest uptake specifc rate was observed for glucose (42.5× 10–12 mmol cell/h) in SF and for glutamine (30.8× 10–12 mmol/cell/h) in STR, in the exponential growth phases. A wave pattern was observed for assessed analytes throughout the infection phase and the glucose had the highest wave amplitude within the 10–10 mmol cell/h order. This alternative uptake and release behavior is in harmony with the lytic cycle of baculovirus in insect cells. The virus propagation and VLP generation were not limited by glucose, glutamine, and glutamate, neither by the toxicity of lactate nor ammonium under the conditions appraised in this work. The fndings from this work can be useful to set baculovirus infection processes at high cell density to improve rabies VLP yield, purity, and productivity.

11.
Vaccines, v. 11, n. 9, 39, 2023.
Artigo em Inglês | SES-SP, SESSP-IBPROD, SES-SP | ID: bud-4762

RESUMO

This work aimed to assess, following upstream optimization in Schott flasks, the scalability from this culture platform to a stirred-tank bioreactor in order to yield rabies-recombinant baculovirus, bearing genes of G (BVG) and M (BVM) proteins, and to obtain rabies virus-like particles (VLP) from them, using Sf9 insect cells as a host. Equivalent assays in Schott flasks and a bioreactor were performed to compare both systems and a multivariate statistical approach was also carried out to maximize VLP production as a function of BVG and BVM’s multiplicity of infection (MOI) and harvest time (HT). Viable cell density, cell viability, virus titer, BVG and BVM quantification by dot-blot, and BVG quantification by Enzyme-Linked Immunosorbent Assay (ELISA) were monitored throughout the assays. Furthermore, transmission electron microscopy was used to characterize rabies VLP. The optimal combination for maximum VLP expression was BVG and BVM MOI of 2.3 pfu/cell and 5.1 pfu/cell, respectively, and 108 h of harvest time. The current study confirmed that the utilization of Schott flasks and a benchtop bioreactor under the conditions applied herein are equivalent regarding the cell death kinetics corresponding to the recombinant baculovirus infection process in Sf9 cells. According to the results, the hydrodynamic and chemical differences in both systems seem to greatly affect the virus and VLP integrity after release.

12.
Proc Bioch, v. 124, p. 189-200, jan. 2023
Artigo em Inglês | SES-SP, SESSP-IBPROD, SES-SP | ID: bud-4718

RESUMO

The technologies used in rabies vaccines manufacturing for human use are based on the inactivated virus platform. An alternative to traditional vaccines is virus-like particles (VLPs). This work aimed to characterize the oxygen uptake and transfer rate parameters throughout recombinant baculovirus (rBV) and rabies VLPs production using Sf9 cells in stirred tank bioreactor (STB) for a better bioprocess understanding and scalability. Four runs in a bench STB were performed: cell culture without infection; cells infected singly with rBV bearing rabies virus glycoprotein (rBVG, multiplicity of infection, MOI=0.1 pfu/cell) and matrix protein (rBVM, MOI=0.1 pfu/cell), and coinfected with BVG and BVM at MOI of 3 and 2 pfu/cell, respectively. The specific oxygen uptake rate () and volumetric oxygen transfer coefficient () were monitored throughout the reactions, as well as viable cell concentration, viability, rBV titers, and protein concentration. According to the results herein, the aeration and agitation systems in a bioreactor at a higher scale could be designed using the criterium for scale-up of constant , without oxygen facilities. Besides, rabies VLPs volumetric yield of 2.8 mg/L with a typical size (55–68 nm) was obtained. These findings suggest a promising bioprocess for rabies VLPs at a commercial scale.

13.
Acta sci., Anim. sci ; 44: e55947, 2022. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1380147

RESUMO

The objective of this study was to evaluate the effect of replacement of corn with pre-dried cassava root silage (CRS) on intake, ingestive behavior, production and composition of goat milk. Five lactating Saanen goats were used, with 42.5 ± 4.2 kg body weight and production of 2 kg milk day-1, distributed in a 5 x 5 Latin square with five treatments: 0, 28.6, 58.0, 81.6 and 100% replacement of corn with CRS in the diet dry matter. Goats were monitored for five periods of 15 days each. The intake of dry matter, crude protein and neutral detergent fiber decreased linearly (p <0.05) with the replacement of corn with CRS. Feeding (348.5 ± 62.1 min.) and rumination (468.8 ± 83.8 min.) times were not influenced (p >0.05) by the replacement of corn with CRS, but the ingestion and rumination efficiencies had a linear increase (p > 0.05). Milk production (2.17 ± 0.45 kg day-1), fat (3.49 ± 0.42%), protein (3.11 ± 0.13%) and lactose (4.47 ± 0,11%) of goat milk were not influenced (p >0.05) by the replacement of corn with CRS. Therefore, it is recommended the total replacement of corn with pre-dried cassava root silage in the diet for dairy goats with an average production of 2 kg day-1.(AU)


Assuntos
Animais , Feminino , Silagem/análise , Cabras/fisiologia , Manihot/química , Leite/química , Controle de Qualidade , Lactação/fisiologia , Ingestão de Alimentos/fisiologia
14.
Vet Parasitol ; 209(1-2): 129-32, 2015 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-25747488

RESUMO

The occurrence of antibodies against Toxoplasma gondii was determined in donkeys, mules, and horses from different regions of Brazil. Serum samples from 304 donkeys (67.11%), 118 horses (26.05%), and 31 mules (6.84%) were analyzed by means of the indirect fluorescent antibody test (cutoff=64). Antibodies against T. gondii were detected in 129 equids (28.47%) (82 donkeys, 32 horses, and 15 mules). Tissue samples from 19 seropositive and 50 seronegative animals were obtained in order to isolate the parasite by means of mouse bioassay, and T. gondii was isolated from a donkey. Through genotypic characterization of the isolate, by means of polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) using 11 genotypic markers, the genotype #163 (TgCkBr220), which has already been described in chickens in Brazil, was identified.


Assuntos
Anticorpos Antiprotozoários/sangue , Equidae , Genótipo , Toxoplasma/genética , Toxoplasmose Animal/imunologia , Animais , Brasil/epidemiologia , Especificidade da Espécie , Toxoplasma/imunologia , Toxoplasmose Animal/sangue , Toxoplasmose Animal/epidemiologia
15.
Eng. sanit. ambient ; 22(2): 251-259, mar.-abr. 2017. tab, graf
Artigo em Português | LILACS | ID: biblio-840406

RESUMO

RESUMO O objetivo deste estudo foi realizar um diagnóstico da qualidade das águas dos igarapés Rabo de Porco e Precuá, localizados na área de implantação da Refinaria Premium I, no município de Bacabeira, Maranhão. Foram avaliadas as características físico-químicas e microbiológicas da água em pontos a montante e a jusante de cada corpo d'água, confrontando os resultados com a Resolução CONAMA nº 357/2005 e com o índice de qualidade da água (IQA). No período de maio a agosto de 2012, foram efetuadas amostragens mensais dos seguintes parâmetros físicos e químicos da água: temperatura, turbidez, pH, sólidos totais (ST), oxigênio dissolvido (OD), demanda bioquímica de oxigênio (DBO5), nitrogênio total, fósforo total (PT) e coliformes fecais. Os resultados mostraram diferenças significantes dos parâmetros entre os pontos a montante e a jusante apenas para as variáveis ST e turbidez, especialmente no Precuá. A análise dos componentes principais (PCA) revelou uma correlação moderada entre as variáveis. Os resultados obtidos enquadraram as águas da maioria dos pontos amostrados predominantemente na classe 3, seguida da classe 2 da Resolução CONAMA nº 357/2005. O IQA evidenciou ligeiras diferenças espaciais e temporais entre os corpos d'água investigados: o igarapé do Rabo de Porco registrou o melhor IQA, enquanto o pior IQA ocorreu na coleta de maio no ponto amostral Precuá a jusante. As informações levantadas servirão de base para avaliações de mudanças nas características e na qualidade dos corpos d'água estudados no decorrer da implantação e operação do empreendimento.


ABSTRACT The aim of this study was to assess the water quality for the creeks Rabo de Porco and Precuá, located in the area of deployment of the Premium I refinery in the municipality of Bacabeira, Maranhão, Brazil. The physico-chemical and microbiological characteristics of the water at points upstream and downstream of each water body were evaluated, comparing the results with the CONAMA Resolution 357/2005 and the water quality index (WQI). In the period from May to August 2012, monthly samples of the following physical and chemical parameters of water were made: temperature, turbidity, pH, total solids, dissolved oxygen, biochemical oxygen demand, total nitrogen, total phosphorus and fecal coliforms. The results showed significant differences in the parameters between upstream and downstream points, only for the parameters total solids and turbidity, especially for the Precuá creek. The principal component analysis revealed a moderate correlation between the parameters. The data indicated that water from most of the points can be classified in the class 3, followed by the class 2 of the CONAMA Resolution 357/2005. The WQI showed slight spatial and temporal differences between the water bodies investigated: Rabo de Porco creek registered the best WQI, while the worst one occurred in sampling at the downstream Precuá point in May. The information registered will be used as a basis for changes evaluation in characteristics and quality of water bodies studied during the deployment and operation of the enterprise.

16.
Cien Saude Colet ; 15 Suppl 1: 1835-40, 2010 Jun.
Artigo em Português | MEDLINE | ID: mdl-20640346

RESUMO

The aim of the present study was to evaluate the fluoride levels in public water supply in São Luís island, Maranhão State, Brazil, during the month of October of 2006. A stratification sampling was applied, considering the water treatment station (06) and the different districts (46). For fluoride analysis it was used a specific ion electrode. The fluoride concentration varied for 0.05 to 0.84 ppm. Only one water treatment station (Italuís) presented an ideal concentration of fluoride. It was concluded that the fluoride levels need adjustments, evidencing that it is essential an external control for monitoring of those levels in public water supply of São Luis.


Assuntos
Fluoretos/análise , Abastecimento de Água/análise , Brasil , Setor Público
17.
Ciênc. cogn ; 20(1): 61-78, mar. 2015. ilus, tab
Artigo em Português | LILACS, Index Psi (psicologia) | ID: biblio-1017156

RESUMO

Este estudo tem como objetivo principal delinear um perfil das pesquisas científicas que versam sobre o julgamento da auditoria. Para isso, foi realizado um estudo descritivo, tendo-se como instrumento de coleta dos dados artigos selecionados em periódicos via portal de periódicos da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Para a seleção dos artigos, foram utilizadas as palavras-chaves: audit, judgement, behavior, accounting e bias. Como técnica de coleta de dados foi realizada análise do artigo, identificando:temática, objetivo, número de autores, tipo de pesquisa e instrumento estatístico utilizado na análise dos resultados. Nos resultados foram identificadas publicações com a existência de coautores, com predominância do tipo de pesquisa experimental e empírico-analítico, sendo as análises de regressão e fatorial os instrumentos mais utilizados. Os estudos revelam preocupação dos autores em verificar a relação do julgamento na auditoria e seus efeitos em questões como o gerenciamento de resultados e relacionadas ao padrão ético, bem como ao grau de independência, confiabilidade, materialidade e, principalmente,de influências comportamentais, tais como o excesso de confiança e alcances no resultado final dos serviços prestados


This study aims at sketching out a profile of scientific papers dealing with judgments in the auditing. A descriptive study was carried out with data gathered from articles in journals found on the CAPES (government agency for higher education training) website. The keywords audit,judgment, behavior, accounting and bias were used for the selection of the articles. As a data collection technique, the articles were scanned for theme, aim, number of authors, type of research and statistical instrument used in the analysis of the results. Results showed the existence ofco-authored publications; most of them were based on experimental and empirical-analytic research; regression and factor analyses were the instruments most used. The studies show a concern by the authors in verifying the relationships between judgments in the auditing and their effects on issues such as results management,issues related to their ethical standards as well as the degree of independence, reliability and materiality, and especially that of behavioral influences such as overconfidence and the scope of the final results of the services provided


Assuntos
Humanos , Viés , Auditoria Financeira , Contabilidade , Moral , Variações Dependentes do Observador , Julgamento Moral Retrospectivo
18.
Cienc. cogn ; 20(1): 61-78, 30 abr 2015. tab, graf
Artigo em Português | Index Psi (psicologia) | ID: psi-67293

RESUMO

Este estudo tem como objetivo principal delinear um perfil das pesquisas científicas que versam sobre o julgamento da auditoria. Para isso, foi realizado um estudo descritivo, tendo-se como instrumento de coleta dos dados artigos selecionados em periódicos via portal de periódicos da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Para aseleção dos artigos, foram utilizadas as palavras-chaves:audit, judgement, behavior, accounting e bias. Como técnica de coleta de dados foi realizada análise do artigo, identificando:temática, objetivo, número de autores, tipo de pesquisa e instrumento estatístico utilizado na análise dos resultados. Nos resultados foram identificadas publicações com a existência de coautores, com predominância do tipo de pesquisa experimental e empírico-analítico, sendo as análises de regressão e fatorial os instrumentos mais utilizados. Os estudos revelam preocupação dos autores em verificar a relação do julgamento na auditoria e seus efeitos em questões como o gerenciamento de resultados e relacionadas ao padrão ético, bem como ao grau de independência, confiabilidade, materialidade e, principalmente,de influências comportamentais, tais como o excesso de confiança e alcances no resultado final dos serviços prestados (AU)


This study aims at sketching out a profile of scientific papers dealing with judgments in the auditing. A descriptive study was carried out with data gathered from articles in journals found on the CAPES (government agency for higher education training) website. The keywords audit,judgment, behavior, accounting and bias were used for the selection of the articles. As a data collection technique, the articles were scanned for theme, aim, number of authors, type of research and statistical instrument used in the analysis of the results. Results showed the existence ofco-authored publications; most of them were based on experimental and empirical-analytic research; regression and factor analyses were the instruments most used. The studies show a concern by the authors in verifying the relationships between judgments in the auditing and their effects on issues such as results management,issues related to their ethical standards as well as the degree of independence, reliability and materiality, and especially that of behavioral influences such as overconfidence and the scope of the final results of the services provided (AU)


Assuntos
Humanos , Contabilidade , Auditoria Financeira , Moral , Viés , Julgamento Moral Retrospectivo , Variações Dependentes do Observador
19.
Rev. bras. ciênc. vet ; 22(3-4): 165-170, jul.-dez.2015. il.
Artigo em Português | LILACS | ID: biblio-997164

RESUMO

Este estudo teve como objetivo descrever casos de abortamento por leptospirose em éguas, ocorrido no município de Mossoró, Rio Grande do Norte. Cinco éguas com histórico de abortamento foram submetidas a exames clínicos, hematológico e pesquisa de aglutininas anti-Leptospira para 22 diferentes sorovares, pelo Teste de Soroaglutinação Microscópica (SAM). Foi realizado exame anatomopatológico da placenta e de um feto equino abortado com 9 meses de idade. A infecção por Leptospira sp. ocorreu em três éguas que abortaram, sendo uma positiva para o sorovar Pomona, título 800; uma para o sorovar Hardjo, título 400; uma para o sorovar Icterohaemorrhagiae, título 400. No exame anatomopatológico da placenta foram observados cotilédones edemaciados de coloração vermelho escuro, com áreas focais enegrecidas, friáveis e necróticos. No exame necroscópico do feto observou-se icterícia, presença de líquido sero-sanguinolento na cavidade abdominal, torácica e saco pericárdico, áreas multifocais de hemorragia no fígado, coração e pulmão, rins de consistência friável e áreas multifocais branco amareladas. Histologicamente, verificou-se necrose e inflamação do epitélio placentário coriônico; necrose difusa de hepatócitos e hepatite mononuclear; acentuada hemorragia no interstício do córtex renal, degeneração hidrópica e necrose das células do epitélio tubular e presença de cilindros hemáticos intratubular; além de nefrite intersticial mononuclear e pneumonia intersticial mononuclear difusa acentuada. Os resultados deste estudo demonstraram títulos elevados de aglutininas anti-Leptospira em éguas e a presença de lesões histológicas nos órgãos fetais e placenta, confirmando a importância da Leptospira como agente causador de abortamento


This study aimed to describe cases of leptospirosis abortions in horses, which occurred in the city of Mossoró, Rio Grande do Norte. Five mares with abortion history, were submitted to clinical, hematological and detection of specific anti-Leptospira agglutinins facing 22 different serotypes, by Microscopic Agglutination Test (MAT). It was performed a pathological examination of the placenta and an equine aborted fetus with 9 months old. The Leptospira sp. infection occurred in three mares that aborted. One was positive for Pomona, title 800; one for the Hardjo serovar, title 400 and one for Icterohaemorrhagiae serovar, title 400. In examination of the placenta were observed swollen cotyledons, dark red color with blackened focal areas, friable and necrotic. In the fetus's autopsy was observed icterus, serous-sanguineous fluid in the abdominal cavity, chest and pericardium, multifocal areas of hemorrhage in the liver, heart, lungs, and friable multifocal yellowish white areas in the kidney. Histologically, it was found necrosis and inflammation of the placental chorionic epithelium; diffuse necrosis hepatocyte and mononuclear hepatitis; severe hemorrhage in the interstitium of the renal cortex, hydropic degeneration and necrosis of the tubular epithelial cells and the presence of haematic intratubular cylinders; mononuclear interstitial nephritis and a severe diffuse interstitial mononuclear pneumonia. The results showed high levels of anti-Leptospira agglutinins in mares and presence of histological lesions in the placenta and fetal organs, confirming the importance of Leptospira as abortion-causing agent.


Assuntos
Animais , Aborto Animal , Reprodução , Cavalos , Leptospira
20.
R. bras. Ci. Vet. ; 22(3-4): 165-170, 2015. ilus, tab
Artigo em Português | VETINDEX | ID: vti-338100

RESUMO

This study aimed to describe cases of leptospirosis abortions in horses, which occurred in the city of Mossoró, Rio Grande do Norte. Five mares with abortion history, were submitted to clinical, hematological and detection of specific anti-Leptospira agglutinins facing 22 different serotypes, by Microscopic Agglutination Test (MAT). It was performed a pathological examination of the placenta and an equine aborted fetus with 9 months old. The Leptospira sp. infection occurred in three mares that aborted. One was positive for Pomona, title 800; one for the Hardjo serovar, title 400 and one for Icterohaemorrhagiae serovar, title 400. In examination of the placenta were observed swollen cotyledons, dark red color with blackened focal areas, friable and necrotic. In the fetuss autopsy was observed icterus, serous-sanguineous fluid in the abdominal cavity, chest and pericardium, multifocal areas of hemorrhage in the liver, heart, lungs, and friable multifocal yellowish white areas in the kidney. Histologically, it was found necrosis and inflammation of the placental chorionic epithelium; diffuse necrosis hepatocyte and mononuclear hepatitis; severe hemorrhage in the interstitium of the renal cortex, hydropic degeneration and necrosis of the tubular epithelial cells and the presence of haematic intratubular cylinders; mononuclear interstitial nephritis and a severe diffuse interstitial mononuclear pneumonia. The results showed highlevels of anti-Leptospira agglutinins in mares and presence of histological lesions in the placenta and fetal organs, confirming the importance of Leptospira as abortion-causing agent.(AU)


Este estudo teve como objetivo descrever casos de abortamento por leptospirose em éguas, ocorrido no município de Mossoró, Rio Grande do Norte. Cinco éguas com histórico de abortamento foram submetidas a exames clínicos, hematológico e pesquisa de aglutininas anti-Leptospira para 22 diferentes sorovares, pelo Teste de Soroaglutinação Microscópica (SAM). Foi realizado exame anatomopatológico da placenta e de um feto equino abortado com 9 meses de idade. A infecção por Leptospira sp. ocorreu em três éguas que abortaram, sendo uma positiva para o sorovar Pomona, título 800; uma para o sorovar Hardjo, título 400; uma para o sorovar Icterohaemorrhagiae, título 400. No exame anatomopatológico da placenta foram observados cotilédones edemaciados de coloração vermelho escuro, com áreas focais enegrecidas, friáveis e necróticos. No exame necroscópico do feto observou-se icterícia, presença de líquido sero-sanguinolento na cavidade abdominal, torácica e saco pericárdico, áreas multifocais de hemorragia no fígado, coração e pulmão, rins de consistência friável e áreas multifocais branco amareladas. Histologicamente, verificou-se necrose e inflamação do epitélio placentário coriônico; necrose difusa de hepatócitos e hepatite mononuclear; acentuada hemorragia no interstício do córtex renal, degeneração hidrópica e necrose das células do epitélio tubular e presença de cilindros hemáticos intratubular; além de nefrite intersticial mononuclear e pneumonia intersticial mononuclear difusa acentuada. Os resultados desteestudo demonstraram títulos elevados de aglutininas anti-Leptospira em éguas e a presença de lesões histológicas nos órgãos fetais e placenta, confirmando a importância da Leptospira como agente causador de abortamento.(AU)


Assuntos
Animais , Feminino , Gravidez , Aborto Animal/diagnóstico , Aborto Animal/patologia , Leptospirose/diagnóstico , Leptospirose/veterinária , Cavalos , Necrose/veterinária
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