RESUMO
BACKGROUND: Multiple sclerosis (MS) is an inflammatory disease of the central nervous system associated with increased oxidative stress (OS) and mitochondrial alterations. Fish oil consumption has neuroprotective, antioxidant and anti-inflammatory effects in patients with relapsing-recurrent MS (RR-MS). OBJECTIVE: To evaluate changes in the hydrolytic activity of ATP synthase and mitochondrial membrane fluidity in patients with RR-MS who receive fish oil or olive oil as a dietary supplement. METHODS: Clinical, controlled, randomized, double-blind trial. Patients consumed fish oil or olive oil for one year. The hydrolytic activity of ATPase and the fluidity of the mitochondrial membrane of platelets were quantified. RESULTS: In patients with RR-MS, a decrease in the fluidity of mitochondrial membranes and an increase in the hydrolytic activity of ATP synthase was observed in comparison with healthy controls. After 6 or 9 months of treatment with fish oil or olive oil, respectively, these values were normalized. CONCLUSION: The consumption of fish oil and olive oil increases the fluidity of the mitochondrial membranes and decreases the catabolic activity of ATP synthase in platelets from patients with RR-MS.
Assuntos
Adenosina Trifosfatases/metabolismo , Óleos de Peixe/farmacologia , Interferon beta/uso terapêutico , Mitocôndrias/enzimologia , Esclerose Múltipla Recidivante-Remitente/tratamento farmacológico , Esclerose Múltipla Recidivante-Remitente/enzimologia , Azeite de Oliva/farmacologia , Adulto , Suplementos Nutricionais , Método Duplo-Cego , Feminino , Humanos , Masculino , Fluidez de Membrana/efeitos dos fármacos , Pessoa de Meia-Idade , Mitocôndrias/efeitos dos fármacosRESUMO
Background: Multiple sclerosis (MS) is an inflammatory disease of the central nervous system associated with increased oxidative stress (OS) and mitochondrial alterations. Fish oil consumption has neuroprotective, antioxidant and anti-inflammatory effects in patients with relapsing-recurrent MS (RR-MS). Objective: To evaluate changes in the hydrolytic activity of ATP synthase and mitochondrial membrane fluidity in patients with RR-MS who receive fish oil or olive oil as a dietary supplement. Methods: Clinical, controlled, randomized, double-blind trial. Patients consumed fish oil or olive oil for one year. The hydrolytic activity of ATPase and the fluidity of the mitochondrial membrane of platelets were quantified. Results: In patients with RR-MS, a decrease in the fluidity of mitochondrial membranes and an increase in the hydrolytic activity of ATP synthase was observed in comparison with healthy controls. After 6 or 9 months of treatment with fish oil or olive oil, respectively, these values were normalized. Conclusion: The consumption of fish oil and olive oil increases the fluidity of the mitochondrial membranes and decreases the catabolic activity of ATP synthase in platelets from patients with RR-MS (AU)
Introducción: la esclerosis multiple (EM) es una enfermedad inflamatoria del sistema nervioso central asociada con estrés oxidativo (EO) y alteraciones mitocondriales. El aceite de pescado tiene efectos neuroprotectores, antioxidantes y antiinflamatorios en pacientes con EM remitente-recurrente (EM-RR). Objetivo: evaluar los cambios en la actividad hidrolítica de la ATPasa y de la fluidez de membrana mitocondrial en pacientes con EM-RR que reciben aceite de pescado o aceite de oliva como suplemento alimenticio. Métodos: ensayo clínico, controlado, aleatorizado, doble ciego. Los pacientes consumieron aceite de pescado o aceite de oliva durante un año. Se cuantifico la actividad hidrolítica de la ATPasa y la fluidez de la membrana mitocondrial de plaquetas. Resultados: en pacientes con EM-RR hay una disminución de la fluidez de las membranas mitocondriales y un incremento de la actividad hidrolítica de la ATPasa en comparación con controles sanos. Después de 6 y 9 meses de tratamiento con aceite de oliva y de aceite de pescado, respectivamente, los valores se normalizaron y se mantuvieron así hasta el fin del estudio. Conclusión: el consumo de aceite de pescado y aceite de oliva incrementan la fluidez de membrana y disminuye la actividad catabólica de la ATP sintasa en pacientes con EM-RR (AU)