Avalanches and Extreme Value Statistics of a Mesoscale Moving Contact Line.

We report direct atomic force microscopy measurements of pinning-depinning dynamics of a circular moving contact line (CL) over the rough surface of a micron-sized vertical hanging glass fiber, which intersects a liquid-air interface. The measured capillary force acting on the CL exhibits sawtoothlike fluctuations, with a linear accumulation of force of slope k (stick) followed by a sharp release of force δf, which is proportional to the CL slip length. From a thorough analysis of a large volume of the stick-slip events, we find that the local maximal force F_{c} needed for CL depinning follows the extreme value statistics and the measured δf follows the avalanche dynamics with a power law distribution in good agreement with the Alessandro-Beatrice-Bertotti-Montorsi (ABBM) model. The experiment provides an accurate statistical description of the CL dynamics at mesoscale, which has important implications to a common class of problems involving stick-slip motion in a random defect or roughness landscape.

Escaping speed of bacteria from confinement.

Microswimmers such as bacteria exhibit large speed fluctuation when exploring their living environment. Here, we show that the bacterium Escherichia coli with a wide range of length speeds up beyond its free-swimming speed when passing through narrow and short confinement. The speedup is observed in two modes: for short bacteria with L <20 µm, the maximum speed occurs when the cell body leaves the confinement, but a flagellar bundle is still confined. For longer bacteria (L ≥ 20 µm), the maximum speed occurs when the middle of the cell, where the maximum number of flagellar bundles locate, is confined. The two speed-up modes are explained by a vanishing body drag and an increased flagella drag-a universal property of an "ideal swimmer." The spatial variance of speed can be quantitatively explained by a simple model based on the resistance matrix of a partially confined bacterium. The speed change depends on the distribution of motors, and the latter is confirmed by fluorescent imaging of flagellar hooks. By measuring the duration of slowdown and speedup, we find that the effective chemotaxis is biased in filamentous bacteria, which might benefit their survival. The experimental setup can be useful to study the motion of microswimmers near surfaces with different surface chemistry.

Wetting Dynamics of a Lipid Monolayer.

Direct measurement and control of the dynamic wetting properties of a lipid-coated water-air interface over a wide range of surface tension variations have many important applications. However, the wetting dynamics of the interface near its partial-to-complete wetting transition has not been fully understood. Here, we report a systematic study of the wetting dynamics of a lipid-coated water-air interface around a thin glass fiber of diameter 1-5 µm and length 100-300 µm. The glass fiber is glued onto the front end of a rectangular cantilever to form a "long-needle" atomic-force-microscope probe. Three surface modifications are applied to the glass fiber to change its wetting properties from hydrophilic to hydrophobic. A monolayer of phospholipid dipalmitoylphosphatidylcholine (DPPC) is deposited on the water-air interface in a homemade Langmuir-Blodgett trough, and the surface tension γL of the DPPC-coated water-air interface is varied in the range of 2.5 ≲ γL ≲ 72 mN/m. From the measured hysteresis loop of the capillary force for the three coated fiber surfaces with varying γL, we observe a sharp transition from partial to complete wetting when γL is reduced to a critical value (γL)c. The obtained values of (γL)c are 27 ± 1 mN/m for a DPPC-coated fiber surface and 23 ± 1 mN/m for an trichloro(1H,1H,2H,2H-perfluorooctyl) silane (FTS)-coated surface. Below (γL)c, the contact angle θ0 of the liquid interface is found to be zero for both hydrophobic fiber surfaces and the corresponding spreading parameter S becomes positive. For the FTS-coated fiber surface, the height of capillary rise exhibits a jump when γL is reduced to (γL)c, which indicates that a rapidly advancing liquid film is formed on the fiber surface when the partial-to-complete wetting transition takes place. Our experiment thus establishes a quantitative method by which many other liquid interfaces coated with polymers, surfactants, and biomolecules (such as proteins and lipids) may be characterized dynamically.

Symptomatic cholelithiasis patients have an increased risk of pancreatic cancer: A population-based study.

BACKGROUND AND AIM: Pancreatic cancer is a fatal disease; currently, the risk factor survey is not suitable for sporadic pancreatic cancer, which has neither family history nor the genetic analysis data. The aim of the present study was to evaluate the roles of cholelithiasis and cholelithiasis treatments on pancreatic cancer risk. METHODS: Symptomatic adult patients with an index admission of cholelithiasis were selected from one million random samples obtained between January 2005 and December 2009. The control group was matched with a 1:1 ratio for sex, age, chronic pancreatitis, and pancreatic cystic disease. Subsequent pancreatic cancer, which we defined as pancreatic cancer that occurred ≥ 6 months later, and total pancreatic cancer events were calculated in the cholelithiasis and control groups. The cholelithiasis group was further divided into endoscopic sphincterotomy/endoscopic papillary balloon dilatation, cholecystectomy, endoscopic sphincterotomy/endoscopic papillary balloon dilatation and cholecystectomy, and no-intervention groups for evaluation. RESULTS: The cholelithiasis group and the matched control group included 8265 adults. The cholelithiasis group contained 86 cases of diagnosed pancreatic cancer, and the control group contained 8 cases (P < 0.001). The incidence rate ratio (IRR) of subsequent pancreatic cancer was significantly higher in the cholelithiasis group than in the control group (IRR: 5.28, P < 0.001). The IRR of subsequent pancreatic cancer was higher in the no-intervention group comparing with cholecystectomy group (IRR = 3.21, P = 0.039) but was similar in other management subgroups. CONCLUSION: Symptomatic cholelithiasis is a risk factor for pancreatic cancer; the risk is similar regardless of the intervention chosen for cholelithiasis.

Favorable gallbladder cancer mortality-to-incidence ratios of countries with good ranking of world's health system and high expenditures on health.

BACKGROUND: The mortality-to-incidence ratio (MIR) is a marker that reflects the clinical outcome of cancer treatment. MIR as a prognostic marker is more accessible when compared with long-term follow-up survival surveys. Theoretically, countries with good health care systems would have favorable outcomes for cancer; however, no report has yet demonstrated an association between gallbladder cancer MIR and the World's Health System ranking. METHODS: We used linear regression to analyze the correlation of MIRs with the World Health Organization (WHO) rankings and total expenditures on health/gross domestic product (e/GDP) in 57 countries selected according to the data quality. RESULTS: The results showed high crude rates of incidence/mortality but low MIR in more developed regions. Among continents, Europe had the highest crude rates of incidence/mortality, whereas the highest age-standardized rates (ASR) of incidence/mortality were in Asia. The MIR was lowest in North America and highest in Africa (0.40 and 1.00, respectively). Furthermore, favorable MIRs were correlated with good WHO rankings and high e/GDP (p = 0.01 and p = 0.030, respectively). CONCLUSIONS: The MIR variation for gallbladder cancer is therefore associated with the ranking of the health system and the expenditure on health.

Mechanical pressure, surface excess, and polar order of a dilute rod-like nanoswimmer suspension: role of swimmer-wall interactions.

The mechanical pressure, surface excess, and polar order of a dilute rod-like nanoswimmer suspension confined by two parallel plates are explored by dissipative particle dynamics. The accumulation and preferred orientation of swimmers near the walls are distinctly shown through the density and polar order distributions for various active force, Fa, values and rod lengths. As Fa is increased, it is interesting to observe that there exists a maximum of the polar order, revealing that the dominant mechanism of the swimmer behavior can be altered by the coupling between the active force and the rod-wall interaction. As a result, the influences of the active force on the swim pressure Π(w)a contributed by the swimmers directly and the surface excess Γ* can be classified into two scaling regimes, natural rotation (weak propulsion) and forced rotation (strong propulsion). Π(w)a and Γ* are proportional to Fa2 in the former regime but become proportional to Fa in the latter regime. For all rod-wall repulsions, the swim pressure of active rods in confined systems Π(w)a always differs from that in unbounded systems Π(b)a which is simply proportional to Fa2 associated with the active diffusivity. That is, unlike thermal equilibrium systems, Π(w)a is not a state function because of the presence of the wall-torque.

Hydrodynamic interaction induced breakdown of the state properties of active fluids.

The mechanical pressure of active fluids in which swimmers are modeled by soft run-and-tumble spheres is investigated by dissipative particle dynamics simulations. The incremental pressure (Π) with respect to the system pressure with inactive swimmers comprises the direct contribution of the swimmers (π) and the indirect contribution of fluids associated with hydrodynamic interactions (HIs). The pressure can be determined from the bulk and confining wall and the former is always less than the latter. The π of dilute active dispersions is proportional to their active diffusivity while Π grows generally with propulsive force and run time. However, Π is always substantially less than π because of negative contributions to pressure by HIs. The wall pressure depends on the swimmer-wall interactions, verifying that pressure is not a state function for active spheres due to the HIs. Owing to the distinct flow patterns, Π varies with the swim-type (pusher and puller) subject to the same run-and-tumble parameters at high concentrations.

Directed drift and fluid pumping of nanoswimmers by periodic rectification-diffusion.

The steady ratchet transport of run-and-tumble nanoswimmers in a 3D microfluidic channel constructed by periodic chambers separated by half-cylinder funnels is explored by dissipative particle dynamics. Two regions in a chamber are identified: rectification and active diffusion. While the concentration gradient is driven by the concentration jump in the rectification region, the ratchet current is dominated by the diffusion rate in the active diffusion region, which is classified into normal and Knudsen types. The former obeys Fick's law and is proportional to va2τ, where va is the self-propulsion velocity and τ the run time. In addition, autonomous pumping of fluids is induced by aligned force dipoles associated with nanoswimmers accumulated near funnels, similar to the mechanism of bacteria carpet. The direction of fluid flow is the same as that of the ratchet current but the former is one order of magnitude smaller than the latter. Thus, the fluid velocity depends on the characteristics of nanoswimmers.

Induced polar order in sedimentation equilibrium of rod-like nanoswimmers.

The active diffusion and sedimentation equilibrium of rod-like nanoswimmers with length L are investigated by dissipative particle dynamics. In the absence of propulsion, the nanorod has the rotational correlation time τθ and mobility µ0, which vary with L. On the basis of the mean squared displacement, the diffusive behavior of rod-like nanoswimmers subject to active force Fa is found to follow (D - D0) ∝ (µ0Fa)(2)τθ, where D0 depicts the Brownian diffusivity. When the nanoswimmer suspension is under the external force Fe, the balance between the downward migration and upward active diffusion yields the sedimentation length δ = D/(µ0Fe), which no longer obeys δ0 = kBT/Fe obtained from the Einstein-Smoluchowski relationship, D0 = µ0kBT. Different from the suspension of passive rods, the polar order is clearly seen for active rods. The local polar order is essentially constant within the distance of about 2δ from the bottom wall but decays as the distance is further increased. In this work, the active Peclet number is small compared to unity and the maximum polar order grows linearly with Fe/Fa. The polar order arises because it is easier for the nanoswimmer with the swimming direction opposite to the external force to escape from the bottom wall.

Diffusion, sedimentation equilibrium, and harmonic trapping of run-and-tumble nanoswimmers.

The diffusion of self-propelling nanoswimmers is explored by dissipative particle dynamics in which a nanoswimmer swims by forming an instantaneous force dipole with one of its nearest neighboring solvent beads. Our simulations mimic run-and-tumble behavior by letting the swimmer run for a time τ, then it randomly changes its direction for the next run period. Our simulations show that the swimming speed (ν(a)) of a nanoswimmer is proportional to the propulsion force and the mobility of a pusher is the same as that of a puller. The effective diffusivity is determined by three methods: mean squared displacement, velocity autocorrelation function, and sedimentation equilibrium. The active colloid undergoes directed propulsion at short time scales but changes to random motion at long time scales. The velocity autocorrelation function decreases with time and becomes zero beyond the run time. Under gravity, the concentration profile of active colloids follows Boltzmann distribution with a sedimentation length consistent with that acquired from the drift-diffusion equation. In our simulation, all three methods yield the same result, the effective diffusivity of an active colloid is the sum of the diffusivity of a passive colloid and ν(a)²τ/6. When the active colloids are confined by a harmonic well, they are trapped within a confinement length defined by the balance between the swimmer active force and restoring force of the well. When the confinement length is large compared to the run length, the stationary density profile follows the Boltzmann distribution. However, when the run length exceeds the confinement length, the density distribution is no longer described by Boltzmann distribution, instead we found a bimodal distribution.

Diffusion and surface excess of a confined nanoswimmer dispersion.

The diffusivity and surface excess of nanoswimmers which are confined in two plates with the separation H are explored by dissipative particle dynamics. Both mean squared displacement and velocity autocorrelation function methods are used to study the diffusive behavior of nanoswimmers with the Brownian diffusivity D0 and the results obtained from both methods are consistent. The active diffusivity of confined nanoswimmers (D - D0) depends on the wall separation, swimming speed v(a), and run time τ. Our simulation results show that (D-D0)/v(a)(2)τ is a function of v(a)τ/H. The reduction in the diffusivity of active colloids is more significant than that of passive particles. The distribution of nanoswimmers between two parallel walls is acquired and two regions can be identified. The accumulation of nanoswimmers near walls is quantitatively described by the surface excess Γ. It is found that Γ grows as the nanoswimmer concentration c(b), swimming speed v(a), and run time τ are increased. The coupling between the ballistic trajectory of nanoswimmers and the walls results in nanoswimmer accumulation. The simulation outcomes indicate that Γ/Hc(b) is a function of H/v(a)τ.

Bidirectional bacterial gliding motility powered by the collective transport of cell surface proteins.

The gliding motility of Flavobacterium johnsoniae is driven by moving surface adhesive proteins. Recently, these motility components were observed to travel along a closed loop on the cell surface. The mechanism by which such moving surface adhesins give rise to cell motion remains unknown. On the basis of the unique motility properties of F. johnsoniae, we present a generic model for bidirectional motion of rigidly coupled adhesins, which are propelled in opposite directions. Using analytical and numerical methods, we demonstrate that, for a sufficiently large adhesin speed, bidirectional motion arises from spontaneous symmetry breaking. The model also predicts that, close to the bifurcation point, a weak asymmetry in the binding dynamics is sufficient to facilitate directed motility, indicating that the direction of motion could be sensitively regulated internally in response to inhomogeneity of the environment.

Coarse-grain simulations of active molecular machines in lipid bilayers.

A coarse-grain method for simulations of the dynamics of active protein inclusions in lipid bilayers is described. It combines the previously proposed hybrid simulations of bilayers [M.-J. Huang, R. Kapral, A. S. Mikhailov, and H.-Y. Chen, J. Chem. Phys. 137, 055101 (2012)], based on molecular dynamics for the lipids and multi-particle collision dynamics for the solvent, with an elastic-network description of active proteins. The method is implemented for a model molecular machine which performs active conformational motions induced by ligand binding and its release after reaction. The situation characteristic for peripheral membrane proteins is considered. Statistical investigations of the effects of single active or passive inclusions on the shape of the membrane are carried out. The results show that the peripheral machine produces asymmetric perturbations of the thickness of two leaflets of the membrane. It also produces a local saddle in the midplane height of the bilayer. Analysis of the power spectrum of the fluctuations of the membrane midplane shows that the conformational motion of the machine perturbs these membrane fluctuations. The hydrodynamic lipid flows induced by cyclic conformational changes in the machine are analyzed. It is shown that such flows are long-ranged and should provide an additional important mechanism for interactions between active inclusions in biological membranes.

Statistical laws of stick-slip friction at mesoscale.

Friction between two rough solid surfaces often involves local stick-slip events occurring at different locations of the contact interface. If the apparent contact area is large, multiple local slips may take place simultaneously and the total frictional force is a sum of the pinning forces imposed by many asperities on the interface. Here, we report a systematic study of stick-slip friction over a mesoscale contact area using a hanging-beam lateral atomic-force-microscope, which is capable of resolving frictional force fluctuations generated by individual slip events and measuring their statistical properties at the single-slip resolution. The measured probability density functions (PDFs) of the slip length δxs, the maximal force Fc needed to trigger the local slips, and the local force gradient [Formula: see text] of the asperity-induced pinning force field provide a comprehensive statistical description of stick-slip friction that is often associated with the avalanche dynamics at a critical state. In particular, the measured PDF of δxs obeys a power law distribution and the power-law exponent is explained by a new theoretical model for the under-damped spring-block motion under a Brownian-correlated pinning force field. This model provides a long-sought physical mechanism for the avalanche dynamics in stick-slip friction at mesoscale.

Nano-swimmers in biological membranes and propulsion hydrodynamics in two dimensions.

Active protein inclusions in biological membranes can represent nano-swimmers and propel themselves in lipid bilayers. A simple model of an active inclusion with three particles (domains) connected by variable elastic links is considered. First, the membrane is modeled as a two-dimensional viscous fluid and propulsion behavior in two dimensions is examined. After that, an example of a microscopic dynamical simulation is presented, where the lipid bilayer structure of the membrane is resolved and the solvent effects are included by multiparticle collision dynamics. Statistical analysis of data reveals ballistic motion of the swimmer, in contrast to the classical diffusion behavior found in the absence of active transitions between the states.

Coarse-grain model for lipid bilayer self-assembly and dynamics: multiparticle collision description of the solvent.

A mesoscopic coarse-grain model for computationally efficient simulations of biomembranes is presented. It combines molecular dynamics simulations for the lipids, modeled as elastic chains of beads, with multiparticle collision dynamics for the solvent. Self-assembly of a membrane from a uniform mixture of lipids is observed. Simulations at different temperatures demonstrate that it reproduces the gel and liquid phases of lipid bilayers. Investigations of lipid diffusion in different phases reveals a crossover from subdiffusion to normal diffusion at long times. Macroscopic membrane properties, such as stretching and bending elastic moduli, are determined directly from the mesoscopic simulations. Velocity correlation functions for membrane flows are determined and analyzed.

Utility of Acute Physiology and Chronic Health Evaluation (APACHE II) in Predicting Mortality in Patients with Pyogenic Liver Abscess: A Retrospective Study.

Pyogenic liver abscess (PLA) is a major life-threatening disease with varied clinical features. This study aimed to determine predictors of mortality in patients with PLA using criteria determined upon admission. We retrospectively examined the data of 324 hospitalized adults in whom liver abscesses were confirmed using abdominal ultrasound and/or computed tomography. The relationship between various risk factors was assessed using multivariate analysis. A total of 109 (33.6%) patients were admitted to the intensive care unit (ICU). The overall mortality rate was 7.4% and was higher among ICU patients than non-ICU patients (21.1% vs. 0.5%, p < 0.001). PLA patients with an Acute Physiology and Chronic Health Evaluation (APACHE) II score ≥18 had a 19.31-fold increased risk, and those with concomitant infections had a 34.33-fold increased risk of 30-day mortality according to multivariate analysis. The estimated area under the receiver operating characteristic curve for predicting 30-day mortality revealed that APACHE II score ≥18 (sensitivity of 75% and specificity of 84%, p < 0.0001) had better discriminative power than Sequential Organ Failure Assessment (SOFA) ≥6 (sensitivity of 81% and specificity of 66%, p < 0.0001). APACHE II has shown better discrimination ability than SOFA in predicting mortality in PLA patients. To improve outcomes in patients with PLA, future management strategies should focus on high-risk patients.

Dynamics of a membrane coupled to an active fluid.

The dynamics of a membrane coupled to an active fluid on top of a substrate is considered theoretically. It is assumed that the director field of the active fluid has rotational symmetry in the membrane plane. This situation is likely to be relevant for in vitro reconstructed actomyosin-membrane system. Different from a membrane coupled to a polar active fluid, this model predicts that only when the viscosity of the fluid above the membrane is sufficiently large, a contractile active fluid is able to slow down the relaxation of the membrane for perturbations with wavelength comparable to the thickness of the active fluid. Hence, our model predicts a finite-wavelength instability in the limit of strong contractility, which is different from a membrane coupled to a polar active fluid. However, a membrane coupled to an extensile active fluid is always unstable against long-wavelength perturbations due to active extensile stress enhanced membrane undulation.