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1.
Dev Sci ; : e13549, 2024 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-39020452

RESUMO

Harlow's seminal work on the nature of attachment focused on the importance of warm, responsive, and loving relationships in children's healthy development. While the need for love and care is arguably universal, the ways in which these emotions are expressed can vary across cultural contexts. We examined how Chinese American parents' expressions of love were associated with children's attachment security. A total of 110 Chinese American immigrant parent-child dyads (children 7-11 years old, 49% girls) participated in 3-min conversations in which parents were instructed to communicate love and care. Proposition-level analyses in speech (total 8825 propositions) identified three types of affection: training (guan and chiao shun); relational affection (qin); and validation (acceptance and encouragement of child's own expression of emotion, thoughts, and behaviors). Higher training was observed in parents with lower American orientation and lower income. Higher relational affection was observed in parents with lower income. Higher validation was observed in parents with higher income. Using path analyses, training and validation were found to be positively associated with children's attachment security beyond parenting styles. Effects of parents' relational affection were moderated by children's American orientation. Results demonstrate how immigrant parents draw on multiple cultural scripts to express love and care. These findings expand traditional concepts of parental love in immigrant families and illustrate how bicultural expressions of love can shape attachment security in middle childhood. A video abstract of this article can be viewed at https://youtu.be/HqUfIDxkFsE RESEARCH HIGHLIGHTS: Analyses of parent-child discussions identified three parental affection styles (i.e., training, relational affection, and validation) as expressions of love and care in Chinese American immigrant families. Training and validation were positively associated with children's attachment security. Relational affection was associated with lower attachment security for children with higher American cultural orientation, suggesting the effects of parent-child expressions of love are shaped by acculturation.

2.
Child Dev ; 92(6): e1126-e1137, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34138465

RESUMO

A central theme of acculturative specificity is the heterogeneity of the immigrant experience. This study integrated this application of the Specificity Principle with intergenerational transmission models of self-regulation and identified both common and specific pathways in the self-regulatory development of Chinese American children in immigrant families (N = 169, Mage = 9.2 years). Consistent with intergenerational transmission models, results indicated associations between parents' and children's effortful control, with the mediation of these associations via authoritarian parenting. Parental education, family income, and children's bilingual proficiency were also uniquely associated with children's executive function and effortful control. Together, findings provide new directions for research with ethnic minority immigrant families, and underscore the utility of within-group approaches in advancing research on ethnic minority children's development.


Assuntos
Emigrantes e Imigrantes , Minorias Étnicas e Raciais , Criança , China , Etnicidade , Humanos , Grupos Minoritários , Relações Pais-Filho , Poder Familiar
3.
Cultur Divers Ethnic Minor Psychol ; 27(1): 72-81, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32352807

RESUMO

OBJECTIVES: The present study examined how English proficiency, aspects of social status (education, income, and shifts in subjective social status), and interpersonal support were directly and indirectly associated with variations in depressive symptoms among Chinese immigrant mothers. METHOD: Individual semistructured interviews and questionnaires were administered to 257 first-generation Chinese immigrant mothers in the United States (Mage = 37.87 years). Participants reported on their English proficiency, perceived shifts in subjective social status, income, education, and levels of interpersonal support. Depressive symptoms were assessed through semistructured individual interviews using a measure previously developed and validated with Chinese American immigrant adults. RESULTS: Path analyses indicated that participants' perceived upward shifts in subjective social status, higher levels of interpersonal support, and higher annual household income were associated with fewer depressive symptoms. Associations between English proficiency and participants' depressive symptoms were mediated by shifts in subjective social status, income, and interpersonal support. CONCLUSION: Chinese immigrant mothers' perceptions of postmigration changes in subjective social status and the availability of interpersonal support play important roles in their mental health, even accounting for objective indicators of socioeconomic status. (PsycInfo Database Record (c) 2021 APA, all rights reserved).


Assuntos
Emigrantes e Imigrantes , Mães , Adulto , Depressão , Feminino , Humanos , Relações Interpessoais , Percepção , Distância Psicológica , Apoio Social , Estados Unidos
4.
J Exp Child Psychol ; 200: 104946, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32791380

RESUMO

Children's executive function (EF) may be negatively affected by their exposure to traumatic events. However, few investigations have examined the relations between EF and the associated developmental outcomes of young children exposed to trauma. Likewise, although growing evidence highlights intergenerational associations between caregiver and child EF, these associations remain unexplored among preschool-aged children exposed to trauma. The current study used a multimethod approach to test the direct and indirect associations of children's trauma symptomatology, caregiver and child EF, and children's concurrent behavioral and cognitive functioning in a sample of trauma-exposed, preschool-aged children (N = 109; Mage = 52.11 months, SD = 12.19) and their caregivers. Results indicated positive associations between children's trauma symptomatology and their behavior problems, positive associations between caregiver and child performance on tasks of EF, and positive associations between children's EF and a latent factor of cognitive functioning indicated by children's performance on standardized tests of IQ and receptive vocabulary. However, the hypothesized indirect effects of caregiver and child EF were not supported. Implications for clinical interventions with young children and families exposed to trauma are discussed.


Assuntos
Cuidadores/psicologia , Comportamento Infantil/psicologia , Cognição/fisiologia , Função Executiva/fisiologia , Família/psicologia , Criança , Maus-Tratos Infantis/psicologia , Pré-Escolar , Exposição à Violência/psicologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Comportamento Problema/psicologia
5.
J Cross Cult Psychol ; 50(3): 381-395, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31543545

RESUMO

Cultural values and social status are two salient factors in the psychological experiences of immigrant families, and both have been associated with immigrant parents' patterns of emotional expression in previous studies. The present study examined how endorsement of cultural values (collectivism and conformity) and social status were uniquely associated with immigrant parents' emotional expressivity in the family. First-generation Chinese American immigrant parents (N = 239, 80% mothers; M = 41.31 years old) of elementary-age children reported on their endorsement of values of collectivism and conformity, their patterns of emotional expressivity in the family context, and their family income and education levels. Path analyses indicated unique positive associations between family income and all domains of parents' emotional expressivity and negative associations between family income and parents' endorsement of collectivism and conformity. Parents' endorsement of collectivism was negatively associated with negative-dominant expressivity. We discuss implications of our findings for theories of culture and emotion, as well as for future intersectional approaches with Asian American populations.

6.
Artigo em Inglês | MEDLINE | ID: mdl-33132738

RESUMO

Though a number of language socialization processes are theorized to promote children's heritage language proficiency (HLP), little research has considered these processes in a single study and examined their prospective relations to multiple domains of HLP in school-age children. In a two-wave longitudinal study of Chinese American children of immigrant parents (N = 258, age = 7-11 years), language socialization processes (e.g., adult HL use at home, parental attitudes towards HL, child participation in HL classes or extracurricular activities) were assessed using parent reports and behavioral observation at Time 1 (1st to 2nd grade). Children's HLP (Cantonese or Mandarin) was assessed using vocabulary and literacy tests at Time 2. Results of structural equation modeling showed that adults' Chinese language use with children at home predicted children's higher Chinese receptive and expressive vocabulary two years later, and children's participation in Chinese language extra-curricular activities predicted their higher Chinese receptive and expressive vocabulary and higher Chinese word reading. By contrast, parental valuing of Chinese language and children's exposure to Chinese media did not predict children's Chinese proficiency. These findings provided support for the benefits of HL use at home and HL classes in promoting HL development in children in immigrant families.

7.
Cultur Divers Ethnic Minor Psychol ; 21(4): 619-29, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25133412

RESUMO

The present study examined 2 measures of Chinese American immigrant parents' emotional expression in the family context: self-reported emotional expressivity and observed emotional expression during a parent-child interaction task. Path analyses were conducted to examine the concurrent associations between measures of emotional expression and (a) parents' American and Chinese cultural orientations in language proficiency, media use, and social affiliation domains, and (b) parents' and teachers' ratings of children's emotion-related regulation. Results suggested that cultural orientations were primarily associated with parents' self-reported expressivity (rather than observed emotional expression), such that higher American orientations were generally associated with higher expressivity. Although parents' self-reported expressivity was only related to their own reports of children's regulation, parents' observed emotional expression was related to both parents' and teachers' reports of children's regulation. These results suggest that self-reported expressivity and observed emotional expression reflect different constructs and have differential relations to parents' cultural orientations and children's regulation.


Assuntos
Asiático/psicologia , Comportamento Infantil/psicologia , Emigrantes e Imigrantes/psicologia , Emoções , Pais/psicologia , Adulto , Criança , China/etnologia , Cultura , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fatores Socioeconômicos , Estados Unidos
8.
Cultur Divers Ethnic Minor Psychol ; 20(2): 202-12, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24041263

RESUMO

Using data from a socioeconomically diverse sample of Chinese American children (n = 258, aged 6-9 years) in immigrant families, we examined the concurrent relations among neighborhood economic disadvantage and concentration of Asian residents, parenting styles, and Chinese American children's externalizing and internalizing problems. Neighborhood characteristics were measured with 2000 U.S. Census tract-level data, parents (mostly mothers) rated their own parenting styles, and parents and teachers rated children's behavioral problems. Path analysis was conducted to test two hypotheses: (a) parenting styles mediate the relations between neighborhood characteristics and children's behavioral problems, and (b) children's behavioral problems mediate the relations between neighborhood and parenting styles. We found that neighborhood Asian concentration was positively associated with authoritarian parenting, which in turn was associated with Chinese American children's higher externalizing and internalizing problems (by parents' reports). In addition, neighborhood economic disadvantage was positively related to children's externalizing problems (by parents' reports), which in turn predicted lower authoritative parenting. The current results suggest the need to consider multiple pathways in the relations among neighborhood, family, and child adjustment, and they have implications for the prevention and intervention of behavioral problems in Chinese American children.


Assuntos
Asiático/psicologia , Comportamento Infantil/etnologia , Emigrantes e Imigrantes/psicologia , Poder Familiar/etnologia , Características de Residência , Adaptação Psicológica , Adulto , Criança , Comportamento Infantil/psicologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Modelos Psicológicos , Mães , Relações Pais-Filho , Poder Familiar/psicologia , Pais , Fatores Socioeconômicos , Inquéritos e Questionários
9.
J Clin Psychol ; 68(7): 829-42, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22623269

RESUMO

OBJECTIVE: The present study proposed and tested a cognitive model of mania and reward. METHOD: Undergraduates (N = 284; 68.4% female; mean age = 20.99 years, standard deviation ± 3.37) completed measures of family goal setting and achievement values, personal reward-related beliefs, cognitive symptoms of mania, and risk for mania. RESULTS: Correlational analyses and structural equation modeling supported two distinct, but related facets of mania-relevant cognition: stably present reward-related beliefs and state-dependent cognitive symptoms in response to success and positive emotion. Results also indicated that family emphasis on achievement and highly ambitious extrinsic goals were associated with these mania-relevant cognitions. Finally, controlling for other factors, cognitive symptoms in response to success and positive emotion were uniquely associated with lifetime propensity towards mania symptoms. CONCLUSIONS: Results support the merit of distinguishing between facets of mania-relevant cognition and the importance of the family in shaping both aspects of cognition.


Assuntos
Transtorno Bipolar/psicologia , Cognição , Família/psicologia , Logro , Transtorno Bipolar/etiologia , Feminino , Humanos , Masculino , Modelos Psicológicos , Testes Psicológicos , Recompensa , Fatores de Risco , Adulto Jovem
10.
J Am Coll Health ; 70(3): 665-669, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-32610035

RESUMO

ObjectiveAsian Women's Action for Resilience and Empowerment (AWARE) is a psychotherapy intervention designed to improve the mental health of Asian American women. This study documented the feasibility and preliminary efficacy of AWARE at three university health service centers in Massachusetts. Participants: 174 female Asian American college/graduate students were screened, and 48 (64%) met the eligibility criteria and enrolled in the study. Methods: This study was a non-randomized, pre-post design at three university/college health service centers in Massachusetts. Retention rates and changes in depression, anxiety, and PTSD symptoms were measured. Results: Results demonstrated high feasibility and significant preliminary efficacy of AWARE across the sites. After the completion of AWARE, participants showed statistically and clinically significant reduction of depression, anxiety, and PTSD symptoms (p-values <.001). Conclusions: This study provides strong evidence that AWARE can be successfully implemented in university settings and provides a promising model of mental health treatment for Asian American women.


Assuntos
Asiático , Psicoterapia de Grupo , Feminino , Serviços de Saúde , Humanos , Estudantes , Universidades
11.
Proteomics ; 10(8): 1658-72, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-20186751

RESUMO

Differential protein profiling by 2-D PAGE is generally useful in biomarker discovery, proteome analysis and routine sample preparation prior to analysis by MS. The goal of this study was to compare 2-D PAGE-resolved protein profile of lymphatic endothelial cells to those of venous, and arterial endothelial cells isolated from lymphatic and blood vessels of bovine mesentery (bm). Three 2-D PAGE electrophoretograms were produced for each of the three cell types and quantitatively analyzed. Protein identification by LC-MS/MS was performed to identify 39 proteins found to be present at statistically significantly different levels in the three cell types (p<0.05). Most of the 39 proteins have not been previously reported in EC proteomic studies of 2-D PAGE electrophoretograms. Three proteins, HSPA1B (HSP70 family member), HSPB1 (HSP27 family member), and UBE2D3 (a member of E2 ubiquitin-conjugating enzymes) found to be at highest levels in bm arterial endothelial cells, bm venous endothelial cells, and bm lymphatic endothelial cells, respectively, were validated by immunoblotting with appropriate antibodies. The lack of substantial overlap between our results and those of other groups' comparative studies are discussed. Functional implications of differences in levels of various proteins identified in the three cell types are also discussed.


Assuntos
Artérias/química , Células Endoteliais/química , Vasos Linfáticos/química , Mesentério/química , Proteoma/análise , Veias/química , Animais , Bovinos , Células Cultivadas , Proteômica
12.
Child Abuse Negl ; 108: 104635, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32739596

RESUMO

The present study used a bioecological framework to examine associations between trauma exposure, trauma-related symptomatology, and executive function (EF) in an urban sample of 88 predominantly ethnic-minority, low-income preschoolers (age 2-5) exposed to interpersonal trauma. Contrary to hypotheses based on past literature documenting associations between trauma exposure and EF deficits in childhood, in regressions adjusting for child gender, family income, and caregiver education, neither trauma exposure or trauma-related symptoms (post-traumatic stress symptoms, internalizing behaviors, or externalizing behaviors) were significantly associated with children's EF performance. Associations between child trauma exposure, symptomatology, and executive function were not moderated by parental PTSD symptomatology; and EF was not differentially predicted by type of trauma. Results suggest that, within an ethnically-diverse sample of preschool-aged children exposed to multiple traumas, associations between trauma exposure, symptomatology, and EF may be particularly nuanced. Keywords: child trauma, posttraumatic stress, executive function, preschool-age children, child mental health.


Assuntos
Função Executiva , Transtornos de Estresse Pós-Traumáticos/psicologia , Cuidadores , Criança , Saúde da Criança , Pré-Escolar , Feminino , Humanos , Masculino , Pais , Pobreza , Análise de Regressão , Transtornos de Estresse Pós-Traumáticos/etiologia
13.
Psychiatr Serv ; 71(8): 868-871, 2020 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-32576121

RESUMO

Children who experience transnational separation (TS) from their parents, often referred to as "satellite babies," endure a relatively common but underdiscussed experience. To date, no evaluations of clinical interventions to specifically support transnationally separated families have been described. This column describes implementation of a group therapy pilot program for parents to address parenting and emotional concerns related to TS at a social services agency in Boston's Chinatown. Parent and therapist interviews indicated increased skills in negotiating parental feelings of guilt, shame, and regret caused by TS. These insights can guide practitioners and researchers who wish to address family separation in their communities.


Assuntos
Povo Asiático/psicologia , Emigrantes e Imigrantes/psicologia , Saúde da Família , Poder Familiar , Pais/psicologia , Isolamento Social , Serviço Social/organização & administração , Boston , Criança , Pré-Escolar , China , Humanos , Lactente , Relações Pais-Filho , Poder Familiar/psicologia , Projetos Piloto , Avaliação de Programas e Projetos de Saúde , Psicologia da Criança
14.
Dev Psychol ; 55(5): 1111-1123, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30652886

RESUMO

Research in developmental psychology has traditionally focused on parents' roles as agents of emotion socialization in their children's socioemotional development. By contrast, little longitudinal research has examined sociocultural mechanisms shaping parents' own emotional development. Immigrant parents are an ideal population in which to examine these processes and advance conceptual models of culture and continuing emotional development in adulthood. Using developmental functionalism and bioecological models of development as theoretical frameworks, the present study examined how immigrant parents' and children's cultural orientations were prospectively related to parents' self-reported emotional expressivity in the family context. Chinese American immigrant parents (n = 210) with elementary-aged children were assessed at two time points approximately 2 years apart. Path analyses using longitudinal panel models indicated that immigrant parents' cultural orientation in various cultural domains (language, social relationships, media) prospectively predicted their emotional expressivity in the family context. Parents' emotional expressivity was also predicted by children's cultural orientation and by discrepancies between their own and their children's cultural orientations. Our results underscore pathways through which immigrants' interactions with the family and broader sociocultural context can contribute to continued emotional development in adulthood. We discuss implications of our findings for developmentally informed approaches to the study of culture and emotion. (PsycINFO Database Record (c) 2019 APA, all rights reserved).


Assuntos
Asiático/psicologia , Cultura , Emoções/fisiologia , Pais/psicologia , Socialização , Criança , Comportamento Infantil/psicologia , Desenvolvimento Infantil , China/etnologia , Emigrantes e Imigrantes/psicologia , Feminino , Humanos , Estudos Longitudinais , Masculino , Relações Pais-Filho , Estudos Prospectivos , Inquéritos e Questionários , Estados Unidos
15.
PLoS One ; 13(10): e0205949, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30339671

RESUMO

Compared to individuals in lower positions of power, higher-power individuals are theorized to be less motivated to attend to social cues. In support of this theory, previous research has consistently documented negative correlations between social class and emotion perception. Prior studies, however, were limited by the size and diversity of the participant samples as well as the systematicity with which social class and emotion perception were operationalized. Here, we examine the generalizability of prior research across 10,000+ total participants. In an initial modest sample, (n = 179), Study 1 partially replicated past results: emotion identification correlated negativity with subjective social class (ß = -0.15, 95% CI = [-0.28,-0.02]) and one of two objective social class measures (participant education ß = -0.15, 95% CI = [-0.03,-0.01]). Studies 2-4 followed up on Study 1's mixed results for objective social class in three much larger samples. These results diverged from past literature. In Study 2, complex emotion identification correlated non-significantly with participant education (ß = 0.02, p = 0.25; 95% CI = [-0.01, 0.05], n = 2,726), positively with childhood family income (ß = 0.03, 95% CI = [0.01,0.06], n = 4,312), and positively with parental education (ß = 0.06, 95% CI = [0.04,0.09], n = 4,225). In Study 3, basic emotion identification correlated positively with participant education (ß = 0.05, 95% CI = [0.02, 0.09]), n = 2,564). In Study 4, basic emotion discrimination correlated positively with participant education (ß = 0.09, 95% CI = [0.05,0.13], n = 2,079), positively with parental education (ß = 0.06, 95% CI = [0.02,0.09], n = 3,225), and non-significantly with childhood family income (ß = 0.2, 95% CI = [0.01,0.07], n = 3,272). Results remained similar when restricting analyses to U.S.-based participants. Taken together, these findings suggest that previously reported negative correlations between emotion perception and social class may generalize poorly past select samples and/or subjective measures of social class. Data from the three large web-based samples used in Studies 2-4 are available at osf.io/jf7r3 as normative datasets and to support future investigations of these and other research questions.


Assuntos
Emoções/fisiologia , Classe Social , Percepção Social , Adulto , Idoso , Idoso de 80 Anos ou mais , Discriminação Psicológica , Escolaridade , Face , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Análise e Desempenho de Tarefas , Adulto Jovem
16.
BMC Cell Biol ; 8: 10, 2007 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-17341311

RESUMO

BACKGROUND: The lymphatic system complements the blood circulatory system in absorption and transport of nutrients, and in the maintenance of homeostasis. Angiopoietins 1 and 2 (Ang1 and Ang2) are regulators of both angiogenesis and lymphangiogenesis through the Tek/Tie-2 receptor tyrosine kinase. The response of endothelial cells to stimulation with either Ang1 or Ang2 is thought to be dependent upon the origin of the endothelial cells. In this study, we examined the effects of the angiopoietins on lymphatic, venous and arterial primary endothelial cells (bmLEC, bmVEC and bmAEC, respectively), which were isolated and cultured from bovine mesenteric vessels. RESULTS: BmLEC, bmVEC and bmAEC cell populations all express Tie-2 and were shown to express the appropriate cellular markers Prox-1, VEGFR3, and Neuropilin-1 that define the particular origin of each preparation. We showed that while bmLECs responded slightly more readily to angiopoietin-2 (Ang2) stimulation, bmVECs and bmAECs were more sensitive to Ang1 stimulation. Furthermore, exposure of bmLECs to Ang2 induced marginally higher levels of proliferation and survival than did exposure to Ang1. However, exposure to Ang1 resulted in higher levels of migration in bmLECs than did to Ang2. CONCLUSION: Our results suggest that although both Ang1 and Ang2 can activate the Tie-2 receptor in bmLECs, Ang1 and Ang2 may have distinct roles in mesenteric lymphatic endothelial cells.


Assuntos
Angiopoietina-1/fisiologia , Angiopoietina-2/fisiologia , Células Endoteliais/fisiologia , Receptor TIE-2/fisiologia , Sequência de Aminoácidos , Animais , Bovinos , Morte Celular , Movimento Celular , Proliferação de Células , Células Cultivadas , Humanos , Camundongos , Dados de Sequência Molecular , Alinhamento de Sequência
17.
Mol Cell Biol ; 23(8): 2658-68, 2003 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-12665569

RESUMO

Tie2/Tek is an endothelial cell receptor tyrosine kinase that induces signal transduction pathways involved in cell migration upon angiopoietin-1 (Ang1) stimulation. To address the importance of the various tyrosine residues of Tie2 in signal transduction, we generated a series of Tie2 mutants and examined their signaling properties. Using this approach in conjunction with a phosphorylation state-specific antibody, we identified tyrosine residue 1106 on Tie2 as an Ang1-dependent autophosphorylation site that mediates binding and phosphorylation of the downstream-of-kinase-related (Dok-R) docking protein. This tyrosine residue is contained within a unique interaction motif for the phosphotyrosine binding domain of Dok-R, and the pleckstrin homology domain of Dok-R further contributes to Tie2 binding in a phosphatidylinositol 3'-kinase-dependent manner. Introduction of a Tie2 mutant lacking tyrosine residue 1106 into endothelial cells interferes with Dok-R phosphorylation in response to Ang1. Furthermore, this mutant is unable to restore the migration potential of endothelial cells derived from mice lacking Tie2. Together, these findings demonstrate that tyrosine residue 1106 on Tie2 is critical for coupling downstream cell migration signal transduction pathways with Ang1 stimulation in endothelial cells.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Proteínas de Transporte/química , Proteínas de Transporte/metabolismo , Fosfoproteínas/química , Fosfoproteínas/metabolismo , Receptores Proteína Tirosina Quinases/química , Receptores Proteína Tirosina Quinases/metabolismo , Motivos de Aminoácidos , Sequência de Aminoácidos , Indutores da Angiogênese/farmacologia , Angiopoietina-1 , Animais , Sítios de Ligação , Células COS , Proteínas de Transporte/genética , Linhagem Celular , Membrana Celular/metabolismo , Movimento Celular/fisiologia , Sequência Consenso , Endotélio Vascular/citologia , Endotélio Vascular/efeitos dos fármacos , Endotélio Vascular/metabolismo , Humanos , Glicoproteínas de Membrana/farmacologia , Mutagênese Sítio-Dirigida , Fosfoproteínas/genética , Fosforilação , Fosfotirosina/química , Estrutura Terciária de Proteína , Receptores Proteína Tirosina Quinases/genética , Receptor TIE-2 , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Transdução de Sinais
18.
Physiol Genomics ; 22(2): 257-67, 2005 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-15899944

RESUMO

The elaboration of the vasculature during embryonic development involves restructuring of the early vessels into a more complex vascular network. Of particular importance to this vascular remodeling process is the requirement of the Tek/Tie2 receptor tyrosine kinase. Mouse gene-targeting studies have shown that the Tie2-deficient embryos succumb to embryonic death at midgestation due to insufficient sprouting and remodeling of the primary capillary plexus. To identify the underlying genetic mechanisms regulating the process of vascular remodeling, transcriptomes modulated by Tie2 signaling were analyzed utilizing serial analysis of gene expression (SAGE). Two libraries were constructed and sequenced using embryonic day 8.5 yolk sac tissues from Tie2 wild-type and the Tie2-null littermates. After tag extraction, 45,689 and 45,275 SAGE tags were obtained for the Tie2 wild-type and Tie2-null libraries, respectively, yielding a total of 21,376 distinct tags. Close to 62% of the tags were uniquely annotated, whereas 10% of the total tags were unknown. Using semiquantitative PCR, the differential expression of eight genes was confirmed that included Elk3, an important angiogenic switch gene which was upregulated in the absence of Tie2 signaling. The results of this study provide valuable insight into the potential association between Tie2 signaling and other known angiogenic pathways as well as genes that might have novel functions in vascular remodeling.


Assuntos
Regulação da Expressão Gênica , Receptor TIE-2/metabolismo , Transdução de Sinais/genética , Animais , Embrião de Mamíferos/metabolismo , Camundongos , Camundongos Knockout , Reação em Cadeia da Polimerase , Transcrição Gênica/genética
19.
Front Psychol ; 5: 1069, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25324795

RESUMO

The present study examined whether bilingualism-related advantages in self-regulation could be observed: (a) among Chinese American immigrant children with varying levels of Chinese and English proficiencies, and (b) across different domains of self-regulation in laboratory, home, and classroom contexts. A socioeconomically diverse sample of first- and second-generation Chinese American immigrant children between ages 7 and 10 (n = 223) was administered assessments of Chinese and English language proficiencies and a multi-method, multi-informant battery of self-regulation measures. Multiple regression analyses suggested that controlling for covariates (child age, gender, and SES), children's bilingualism-related advantages were limited to higher performance only on computerized tasks of cognitive flexibility, and only among children with higher degrees of fluency in both Chinese and English. By contrast, proficiencies in one language (either Chinese or English) were uniquely and positively associated with other domains of self-regulation, including parent and teacher-reported effortful control. These results suggest that the bilingual advantage for self-regulation may be observed as a continuous variable among immigrant children with varying levels of bilingual fluency; however, this advantage may not extend across all domains and contexts of self-regulation.

20.
Dev Psychol ; 50(1): 189-201, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23566081

RESUMO

Direct and indirect/mediated relations of (a) children's and parents' cultural orientations and (b) parent-child gaps in cultural orientations to children's psychological adjustment were examined in a socioeconomically diverse sample of 258 Chinese American children (age = 6-9 years) from immigrant families. Parents reported on children's and their own Chinese and American orientations in language proficiency, media use, and social relationships. Parents and teachers rated children's externalizing and internalizing problems and social competence. Using structural equation modeling, we found evidence for both the effects of children's and parents' cultural orientations and the effects of parent-child gaps. Specifically, children's American orientations across domains were associated with their better adjustment (especially social competence). These associations were partly mediated by authoritative parenting. Parents' English and Chinese media use were both associated with higher authoritative parenting, which in turn was associated with children's better adjustment. Furthermore, greater gaps in parent-child Chinese proficiency were associated with children's poorer adjustment, and these relations were partly mediated by authoritative parenting. Together, the findings underscore the complex relations between immigrant families' dual orientations to the host and heritage cultures and children's psychological adjustment.


Assuntos
Adaptação Psicológica , Asiático/psicologia , Cultura , Emigrantes e Imigrantes/psicologia , Orientação/fisiologia , Relações Pais-Filho , Criança , Feminino , Humanos , Masculino , Modelos Psicológicos , Multilinguismo , Pais/psicologia , Inquéritos e Questionários
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