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The Optica Topical Meeting on Digital Holography and 3D Imaging (DH) was held 14-17 August 2023 in Boston, Massachusetts. The meeting was organized co-jointly with the Optica Imaging Congress. Feature issues based on the DH meeting series have been released by Applied Optics (AO) since 2007. Since 2017, AO and the Journal of the Optical Society of America A (JOSA A) have presented a feature issue in each journal. This feature issues includes 17 papers in AO and 9 in JOSA A. Together they cover a large range of topics, reflecting the rapidly expanding techniques and applications of digital holography and 3D imaging. The upcoming DH Conference (DH 2024) will be held from 3 to 6 June in Paestum, Italy.
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The Optica Topical Meeting on Digital Holography and 3D Imaging (DH) was held 14-17 August 2023 in Boston, Massachusetts. The meeting was organized co-jointly with the Optica Imaging Congress. Feature issues based on the DH meeting series have been released by Applied Optics (AO) since 2007. Since 2017, AO and the Journal of the Optical Society of America A (JOSA A) have presented a feature issue in each journal. This feature issues includes 17 papers in AO and 9 in JOSA A. Together they cover a large range of topics, reflecting the rapidly expanding techniques and applications of digital holography and 3D imaging. The upcoming DH Conference (DH 2024) will be held from 3 to 6 June in Paestum, Italy.
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This study presents a novel label-free approach for characterizing cell death states, eliminating the need for complex molecular labeling that may yield artificial or ambiguous results due to technical limitations in microscope resolution. The proposed holographic tomography technique offers a label-free avenue for capturing precise three-dimensional (3D) refractive index morphologies of cells and directly analyzing cellular parameters like area, height, volume, and nucleus/cytoplasm ratio within the 3D cellular model. We showcase holographic tomography results illustrating various cell death types and elucidate distinctive refractive index correlations with specific cell morphologies complemented by biochemical assays to verify cell death states. These findings hold promise for advancing in situ single cell state identification and diagnosis applications.
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Morte Celular , Holografia , Imageamento Tridimensional , Tomografia , Holografia/métodos , Tomografia/métodos , Imageamento Tridimensional/métodos , Humanos , Refratometria/métodosRESUMO
This feature issue is a continuation of a tradition to follow the conclusion of the Optica Topical Meeting on Digital Holography and 3D Imaging (DH+3D). It addresses current research topics in digital holography and 3D imaging that are also in line with the topics of Applied Optics and Journal of the Optical Society of America A.
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This feature issue is a continuation of a tradition to follow the conclusion of the Optica Topical Meeting on Digital Holography and 3D Imaging (DH+3D). It addresses current research topics in digital holography and 3D imaging that are also in line with the topics of Applied Optics and Journal of the Optical Society of America A.
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Digital inline pump-probe holography can be applied to estimate parameters of samples' optical nonlinear properties. Here we propose a mathematical model to describe noncollinear degenerate phase modulation in samples with inhomogeneities of nonlinear refractive index over all three dimensions, namely, two-layered samples and samples with local impurities. The impact of sample parameters in the considered configurations is analyzed. We show that analysis of inline digital holograms obtained by time-resolved inline digital holography can be successfully used for rapid detection and characterization of various types of nonlinear refractive index inhomogeneities.
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Holographic tomography (HT) is an advanced label-free optical microscopic imaging method used for biological studies. HT uses digital holographic microscopy to record the complex amplitudes of a biological sample as digital holograms and then numerically reconstruct the sample's refractive index (RI) distribution in three dimensions. The RI values are a key parameter for label-free bio-examination, which correlate with metabolic activities and spatiotemporal distribution of biophysical parameters of cells and their internal organelles, tissues, and small-scale biological objects. This article provides insight on this rapidly growing HT field of research and its applications in biology. We present a review summary of the HT principle and highlight recent technical advancement in HT and its applications.
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Holografia/instrumentação , Holografia/métodos , Microscopia/instrumentação , Microscopia/métodos , Animais , Linhagem Celular , Simulação por Computador , Humanos , Processamento de Imagem Assistida por Computador , Imageamento Tridimensional , Lasers , Metaboloma , Modelos Químicos , Organelas/ultraestrutura , Refratometria , Análise de Célula Única , SoftwareRESUMO
Data acquisition and processing is a critical issue for high-speed applications, especially in three-dimensional live cell imaging and analysis. This paper focuses on sparse-data sample rotation tomographic reconstruction and analysis with several noise-reduction techniques. For the sample rotation experiments, a live Candida rugosa sample is used and controlled by holographic optical tweezers, and the transmitted complex wavefronts of the sample are recorded with digital holographic microscopy. Three different cases of sample rotation tomography were reconstructed for dense angle with a step rotation at every 2°, and for sparse angles with step rotation at every 5° and 10°. The three cases of tomographic reconstruction performance are analyzed with consideration for data processing using four noise-reduction techniques. The experimental results demonstrate potential capability in retaining the tomographic image quality, even at the sparse angle reconstructions, with the help of noise-reduction techniques.
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Holografia/instrumentação , Holografia/métodos , Tomografia/instrumentação , Tomografia/métodos , Aprendizado Profundo , Processamento de Imagem Assistida por Computador , Imageamento Tridimensional , Pinças Ópticas , Rotação , Saccharomycetales , Razão Sinal-RuídoRESUMO
This feature issue of Applied Optics is dedicated to the international meeting of Information Photonics 2020 (IP'20), which was held September 11-12, 2020, in Taipei, Taiwan. IP'20 covered a broad range of topics, including advanced display techniques, optical computing, and optical storage. This feature issue, however, limits topics to unconventional imaging techniques, such as digital holography, artificial-intelligence associated imaging, compressive imaging, and single-pixel imaging.
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The study focuses on a methodology providing noninvasive monitoring and evaluation of the antitumor effect of traditional Chinese medicine, cantharides complex (canth), on 4T1 breast tumor cells. Digital holographic tomography (DHT) and developed data post-processing algorithms were used for quantitative estimation of changes in optical and morphological parameters of cells. We calculated and compared data on the refractive index, thickness, and projected area of 4T1 breast tumor cells in control untreated specimens and those treated with doxorubicin hydrochloride (DOX), canth, and their combinations. Post-treatment changes in cellular morphology recorded by DHT demonstrated that the two drugs led to noticeably different morphological changes in cells that can be presumably associated with different pathways of their death, apoptosis, or necrosis. The effect of combined treatment with these two drugs strongly depended on their relative concentrations and could lead to changes characteristic either for DOX or for canth; however, being more profound than those obtained when using each drug solely. The results obtained by DHT are in a good correspondence with commonly used cell viability analysis and immunofluorescent analysis of changes in cellular cytoskeleton.
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Antineoplásicos/farmacologia , Neoplasias da Mama/tratamento farmacológico , Cantaridina/farmacologia , Holografia/métodos , Tomografia/métodos , Animais , Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia , Neoplasias da Mama/diagnóstico por imagem , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Doxorrubicina/farmacologia , Feminino , Imunofluorescência , Medicina Tradicional Chinesa , Camundongos , Refratometria/métodosRESUMO
This paper proposes one-shot synthetic aperture digital holographic microscopy using a combination of angular-multiplexing and coherence gating. The proposed angular-multiplexing technique uses multiple noncoplanar incident beams into the synthetic aperture to create tight packed passbands so as to extend spatial frequency spectrum. Coherence gating is performed to prevent the self-interference among the multiple beams. Based on the design guideline proposed herein, a phase-only spatial light modulator is employed as an adjustable blazed grating to split multiple noncoplanar beams and perform angular-multiplexing, and then using coherence gating based on low-coherence-light, superresolution imaging is achieved after one-shot acquisition.
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This study proposes a novel data augmentation method based on numerical focusing of digital holography to boost the performance of learning-based pattern classification. To conduct digital holographic data augmentation (DHDA), a complex pattern diffraction approach is used to provide the least separation of confusion in the effective diffraction regime to access the full-field wavefront information of a target sample. By using DHDA, the accessible amount of labeled data is increased to complement the data manifold and to provide various three-dimensional diffraction characteristics for improving the performance of learning-based pattern classification. Experimental results demonstrated that overall accuracy of pattern classification with DHDA (95.1%) was higher than that without DHDA (90.9%).
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This paper proposes a coded aperture structured illumination (CASI) technique in digital holographic microscopy (DHM). A CASI wave is generated using two binary phase codes (0° and 120°) for spatial phase shifting. The generated CASI wave then interferes with a reference wave to form a coded Fresnel hologram at a single exposure with compressive sensing (CS) to avoid the temporal phase-shifting process of the structured illumination (SI). The CS algorithm is applied to retrieve the missing data of decoded phase-shifted SI-modulated waves, which are used to separate overlapped spatial frequencies for obtaining a larger spatial frequency coverage to provide superresolution imaging. Two phase-only spatial light modulators are applied to generate a directional SI pattern for obtaining a coded aperture with a suitable size to perform one-shot acquisition in the DHM system.
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This study presents a time-resolved imaging technique for detecting ultrafast events in the sample with tunable tick-tock pulses in common-path digital holographic microscopy. The tick-tock pulses are generated from the same single femtosecond pulse source and manipulated through a spatial-multiplexing encoding/decoding scheme with two complementary binary codes for digital hologram recording and reconstruction. The spatial-multiplexing encoding/decoding scheme with compressive sensing on the Fresnel digital hologram is used to recover missing data and achieve high-fidelity wavefront reconstruction. The elapsed time of tick-tock pulses can be adjusted by changing the optical path difference between the pulse pair arms for dynamic observation at various timescales. The proposed method is applied to explore the ultrafast physical phenomenon by quantitative phase imaging with a stopwatch-like tunable timescale ranging from nanoseconds to femtoseconds.
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This study presents a novel tomographic imaging technique for living biomedical samples using an optically driven full-angle rotation scheme based on digital holographic microscopy, in which the three-dimensional refractive index distribution inside the sample can be measured and analyzed. To accomplish the full-angle sample rotation, two optical traps are driven by highly focused spots on the top and bottom of the sample. The rim image of the sample outside the focal depth at the different rotation angles and propagation distances can be corrected and compensated, respectively, via numerical focusing; therefore, tomographic imaging of the sample can be conducted. The proposed approach shows that an entire symmetric spectrum can be acquired for tomographic reconstruction without the missing apple core problem as in traditional sample-rotation schemes. The three-dimensional refractive index of living yeast in a fluid medium is measured and verified.
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Holografia/métodos , Microscopia/métodos , Rotação , Tomografia Óptica/métodosRESUMO
In this study, we introduce what we believe is a novel holographic optical element called a chiral square Fresnel zone plate (CSFZP). The chirality is imposed on a square Fresnel zone plate (SFZP) using a nonclassical technique by rotating the half-period zones relative to one another. The rotation of the half-period zones, in turn, twists the side lobes of the diffraction pattern without altering the focusing properties inherent to a SFZP. As a consequence, the beam profile is hybrid, consisting of a strong central Gaussian focal spot with gradient force similar to that generated by a lens and twisted side lobes with orbital angular momentum. The optical fields at the focal plane were calculated and found to possess a whirlpool-phase profile and a twisted intensity profile. Analysis of the field variation along the direction of propagation revealed a spiraling phase and amplitude distribution. Poynting vector plot of the fields revealed the presence of angular momentum in the regions of chiral side lobes. The phase of the CSFZPs were displayed on a phase-only reflective spatial light modulator and illuminated using a laser. The intensity patterns recorded in the experiment match the calculated ones, with a strong central focal spot and twisted side lobes. The beam pattern was implemented in an optical trapping experiment and was found to possess particle trapping capabilities.
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This work presents a novel hardware phase-unwrapping architecture for digital holographic microscopy. The architecture is based on an iterative region-referenced algorithm because of its simplicity and effectiveness for phase unwrapping. The architecture therefore consumes fewer hardware resources for very large-scale integration implementation. In addition, a novel data reuse scheme is adopted for reducing the memory bandwidth required by the architecture. The architecture can then have fast computation speed for the iterative operations. The architecture has been implemented by field programmable gate array. It acts as a hardware accelerator in an embedded system developed by a network-on-chip platform for performance measurement. The superiorities of the proposed architecture have been confirmed by the experiments.
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This study describes the overlapping of spatial frequency bands for synthetic aperture in digital holography using spectrum normalization to effectively enhance the spatial resolutions of image reconstruction. Synthesized spectrum swelling induced by excessive frequency overlaps can be normalized through the inverse apodization of an adjustable window function, which is similar to the effects of suppressing low-frequency expansion and strengthening high-frequency components of the reconstructed images. The results indicated that using the normalized spectrum synthesis that requires only a few frequency bands effectively enhances the spatial resolution and phase sensitivity of reconstructed images in digital holographic microscopy.
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A secure watermarking algorithm for hologram authentication is presented in this paper. The algorithm exploits the noise-like feature of holograms to randomly embed a watermark in the domain of the discrete cosine transform with marginal degradation in transparency. The pseudo random number (PRN) generators based on a cellular automata algorithm with asymmetrical and nonlocal connections are used for the random hiding. Each client has its own unique PRN generators for enhancing the watermark security. In the proposed algorithm, watermarks are also randomly generated to eliminate the requirements of prestoring watermarks in the clients and servers. An authentication scheme is then proposed for the algorithm with random watermark generation and hiding.
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Therapeutic use of Chinese herbal medicines (CHMs) is a new approach to treat neurodegenerative diseases, such as Alzheimer's disease and Parkinson's disease. The detection of soma volume and neurite outgrowth of living neurons is a highly relevant biomarker related to various application fields, including therapy efficacy and drug safety evaluation. Through the use of digital holographic microscopy (DHM), we may evaluate the therapeutic effect of CHMs in curing neurodegeneration. Panax ginseng has been used in traditional Chinese herbal medicine for centuries. In this study, DHM is applied to monitor the three-dimensional morphology change of retinoic acid-induced human neuroblastoma SH-SY5Y cells during Panax ginseng treatment. We demonstrate the capability of DHM to detect noninvasively SH-SY5Y cell apoptosis and rescue through the measurement of neuronal volume and neurite outgrowth regulation without any labeling reagent. Through DHM, we observed the phase images of the rapidly shrinking cells with decreasing soma volume and shortening neurite outgrowth during glutamate treatments. Then shrinkage in glutamate-induced cells is significantly alleviated during Panax ginseng treatment. The results through DHM are consistent with the result from MTT assay for assessing cell viability during Panax ginseng treatment. Thus, we suggest that application of DHM for measuring soma volume and neurite outgrowth of living neurons may be one appropriate therapeutic evaluation for CHMs.