RESUMO
Candida nivariensis is a cryptic fungal species classified within the Candida glabrata complex. It was described for the first time in 2005 by the means of DNA sequencing. We report a rare case of C. nivariensis deep-seated infection occurring in a 77-year-old man hospitalized for cysto-prostatectomy. Phenotypic testing based on the direct examination and the macroscopic features of the in vitro culture initially suggested C. glabrata species, while MALDI-TOF mass spectrometry enables correct identification. The isolate was found resistant to fluconazole, like in almost 20% of the reported cases. Herein, we present our practical strategy to reliably characterize this rare cryptic species. To date, MALDI-TOF mass spectrometry-based analysis showed very good results for such a purpose.
Assuntos
Candidemia/microbiologia , Saccharomycetales/classificação , Saccharomycetales/isolamento & purificação , Adenocarcinoma de Pulmão/complicações , Adenocarcinoma de Pulmão/imunologia , Adenocarcinoma de Pulmão/microbiologia , Idoso , Candidemia/etiologia , Carcinoma de Células de Transição/microbiologia , Carcinoma de Células de Transição/patologia , França , Humanos , Neoplasias Pulmonares/complicações , Neoplasias Pulmonares/imunologia , Neoplasias Pulmonares/microbiologia , Masculino , Testes de Sensibilidade Microbiana , Técnicas de Tipagem Micológica/métodos , Recidiva , Neoplasias da Bexiga Urinária/microbiologia , Neoplasias da Bexiga Urinária/patologia , Urotélio/patologiaRESUMO
In January 2016, a large-scale outbreak of acute gastroenteritis was reported among French armed forces deployed in the Central African Republic. Challenging investigations, conducted from France, made it possible to identify a norovirus genogroup II in both stool and food samples, confirming a norovirus foodborne disease outbreak. Infected food handler management is discussed.
Assuntos
Infecções por Caliciviridae/virologia , Doenças Transmitidas por Alimentos/virologia , Gastroenterite/virologia , Norovirus/isolamento & purificação , Adulto , Infecções por Caliciviridae/epidemiologia , República Centro-Africana/epidemiologia , Surtos de Doenças , Fezes/virologia , Feminino , Doenças Transmitidas por Alimentos/epidemiologia , Gastroenterite/epidemiologia , Genótipo , Humanos , Masculino , Instalações Militares , Militares/estatística & dados numéricos , Norovirus/genética , RNA Viral/genética , Recursos Humanos , Adulto JovemRESUMO
The ability of human lymphoblastoid cells to secrete large amounts of biologically active human hematopoietic growth factors from adenovirus-based expression vectors was investigated. The gene for human erythropoietin (EPO) was inserted into integrative (pTS39) and episomal (pTS53) vectors. Cell clones, originating from pTS39 or pTS53-transfected and stably selected cells, secreted recombinant human EPO (re-hEPO) at similar levels. The highest production, 60 mu/10(6) cells per 24 h, was obtained from a subclone of pTS39-transfected cells, grown in nonselective medium. The re-hEPO was shown to be biologically active in vivo by incorporation of 59Fe into red blood cells of polycythemic mice and in vitro by the proliferative response of the EPO-dependent cell line UT7. The purified protein of 36 kDa in SDS-PAGE slightly differed from re-hEPO from CHO cells. pTS39 vector was integrated at 15-30 copies per genome, whereas the pTS53 vector replicated at 10 copies per cell. Genes encoding human interleukin-3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) were also expressed in the integrative system as biologically active growth factors, demonstrating that our host-vector system allows the expression of any little gene or cDNA and efficient secretion of the re-protein produced.