RESUMO
<p><b>OBJECTIVE</b>To develop an HILIC method for determination of dencichine in Sanqi tablet and evaluate the quality of Sanqi tablet of different hatches from various manufactures in the market.</p><p><b>METHOD</b>The chromatographic separation was conducted on a Thermo HILIC column (4.6 mm x 250 mm, 5 microm) kept at 25 degrees C with acetonitrile and 0.1% H3PO4 (60:40) as the mobile phase. The flow rate was set at 1 mL x min(-1) and the detection wavelength was set at 213 nm.</p><p><b>RESULT</b>The contents of dencichine in Sanqi tablet ranged from 1.60 to 4.31 mg x g(-1).</p><p><b>CONCLUSION</b>The well established method was successfully applied to determine dencichine in Sanqi tablet. The results demonstrated that this method was simple, accurate and could be applied for quality control of Sanqi as well as its associated preparations.</p>