Parental care behaviour in response to perceived paternity is not mediated by 11-ketotestosterone in bluegill sunfish.

Parental care is critical for the survival of many young animals, but parental care can be costly to the individual providing care. To balance this cost, parents can allocate their care to offspring based on their value, which can be dependent on the offspring's relatedness to the parent. Bluegill (Lepomis macrochirus) is a fish characterized by uniparental male care and high levels of cuckoldry. While parental males of this species have been shown to adaptively adjust their care in response to paternity, the mechanisms for this adjustment are not well understood. Androgens are steroid hormones that are associated with parental care behaviours in many species including bluegill. Here, we test the hypothesis that circulating androgen concentrations mediate the adjustment in care provided by bluegill parental males by manipulating perceived paternity and then measuring circulating 11-ketotestosterone concentration and parental care behaviour. We show that males with higher perceived paternity provide higher levels of nurturing and nest defense behaviour, but contrary to expectations, we found that these males had lower concentrations of 11-ketotestosterone. Furthermore, we found positive correlations between individual circulating plasma 11-ketotestosterone concentrations and nurturing behaviour, but not with the aggressive behaviours that differed between paternity treatments. While bluegill make behavioural changes in response to perceived paternity, these changes do not appear to be modulated by 11-ketotestosterone.

Detection of Circulating Tumor DNA After Stereotactic Ablative Radiotherapy in Patients With Unbiopsied Lung Tumors (SABR-DETECT).

For patients with stage I/IIA non-small-cell lung cancer (NSCLC), surgical resection is the standard treatment. However, some of these patients are not candidates for surgery or refuse a surgical option. Definitive stereotactic ablative radiotherapy (SABR) is a standard approach in these patients. Approximately 15% of patients undergoing SABR for localized NSCLC will experience a recurrence within 2 years. Furthermore, many of these patients are deemed appropriate for SABR without a tissue diagnosis, based on the likelihood of malignancy which can be calculated by validated models. A liquid biopsy, detecting ctDNA, would be useful in early detection of recurrences, and documenting a cancer diagnosis in patients without a biopsy. This is a multi-institutional study enrolling patients with suspected stage I/IIA NSCLC and a pretreatment likelihood of malignancy of ≥60% using the validated models for patients without a tissue diagnosis, in cohort 1 (n = 45). The second cohort will consist of biopsied patients (n = 30-60). SABR will be delivered as per risk-adapted protocol. Plasma will be collected for ctDNA analysis prior to the first fraction of SABR, 24 to 72 hours after first fraction, and at 3, 6, 9, 12, 18, and 24-months. The patients will be followed up with imaging at 3, 6, 9, 12, 18, and 24-months. The primary objective is to assess whether a cancer detection liquid biopsy platform can predict recurrence of NSCLC. The secondary objectives are to assess the impact of SABR on detection rates of ctDNA in patients undergoing SABR and to correlate ctDNA positivity and pretreatment probability of malignancy (NCT05921474).

Leptin administration does not influence migratory behaviour in white-throated sparrows (Zonotrichia albicollis).

Migratory flights by birds are among the most energetically demanding forms of animal movement, and are primarily fueled by fat as an energy source. Leptin is a critical fat-regulation hormone associated with energy balance in non-avian species but its function in birds is highly controversial. Prior research indicated the effects of leptin differed between birds in migratory condition or not, but no research has assessed the effect of leptin on migratory behaviour itself. In this study, our objective was to determine if leptin affects migratory restlessness and fat deposition in migratory songbirds. We used photoperiod manipulation to induce spring migratory condition, and measured migratory restlessness in leptin-injected and saline-injected white-throated sparrows (Zonotrichia albicollis). Leptin treatment had no effect on migratory restlessness nor fat deposition, providing evidence that leptin does not influence avian migratory motivation or behaviour. Our results also further support the idea that birds in a hyperphagic migratory condition may be insensitive to leptin.