RESUMO
Using both sequence- and function-based metagenomic approaches, multiple antibiotic resistance determinants were identified within metagenomic libraries constructed from DNA extracted from bacterial chromosomes, plasmids, or viruses within an activated sludge microbial assemblage. Metagenomic clones and a plasmid that in Escherichia coli expressed resistance to chloramphenicol, ampicillin, or kanamycin were isolated, with many cloned DNA sequences lacking any significant homology to known antibiotic resistance determinants.
Assuntos
DNA Bacteriano/genética , DNA Viral/genética , Farmacorresistência Bacteriana , Metagenoma , Plasmídeos , Esgotos/microbiologia , Ampicilina/farmacologia , Antibacterianos/farmacologia , Cloranfenicol/farmacologia , Escherichia coli/genética , Biblioteca Gênica , Canamicina/farmacologia , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Análise de Sequência de DNARESUMO
The viral metagenome within an activated sludge microbial assemblage was sampled using culture-dependent and culture-independent methods and compared to the diversity of activated sludge bacterial taxa. A total of 70 unique cultured bacterial isolates, 24 cultured bacteriophages, 829 bacterial metagenomic clones of 16S rRNA genes, and 1,161 viral metagenomic clones were subjected to a phylogenetic analysis.