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1.
Cell ; 161(2): 374-86, 2015 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-25799384

RESUMO

Cell movement has essential functions in development, immunity, and cancer. Various cell migration patterns have been reported, but no general rule has emerged so far. Here, we show on the basis of experimental data in vitro and in vivo that cell persistence, which quantifies the straightness of trajectories, is robustly coupled to cell migration speed. We suggest that this universal coupling constitutes a generic law of cell migration, which originates in the advection of polarity cues by an actin cytoskeleton undergoing flows at the cellular scale. Our analysis relies on a theoretical model that we validate by measuring the persistence of cells upon modulation of actin flow speeds and upon optogenetic manipulation of the binding of an actin regulator to actin filaments. Beyond the quantitative prediction of the coupling, the model yields a generic phase diagram of cellular trajectories, which recapitulates the full range of observed migration patterns.


Assuntos
Actinas/metabolismo , Movimento Celular , Modelos Biológicos , Animais , Linhagem Celular , Polaridade Celular , Células Cultivadas , Citoesqueleto/metabolismo , Humanos , Camundongos Endogâmicos C57BL , Oryzias
2.
Biophys J ; 120(12): 2394-2399, 2021 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-33961867

RESUMO

Protein aggregation is of particular interest because of its connection with many diseases and disorders. Many factors can alter the dynamics and result of this process, one of them being the diffusivity of the monomers and aggregates in the system. Here, we study experimentally and theoretically an aggregation process in cells, and we identify two distinct physical timescales that set the number and size of aggregates. The first timescale involves fast aggregation of small clusters freely diffusing in the cytoplasm, whereas in the second one, the aggregates are larger than the pore size of the cytoplasm and thus barely diffuse, and the aggregation process is slowed down. However, the process is not entirely halted, potentially reflecting a myriad of active but random forces that stir the aggregates. Such a slow timescale is essential to account for the experimental results of the aggregation process. These results could also have implications in other processes of spatial organization in cell biology, such as phase-separated droplets.


Assuntos
Agregados Proteicos
3.
J Cell Sci ; 132(24)2019 12 13.
Artigo em Inglês | MEDLINE | ID: mdl-31836687

RESUMO

Many studies have investigated the processes that support polarity establishment and maintenance in cells. On the one hand, polarity complexes at the cell cortex and their downstream signaling pathways have been assigned as major regulators of polarity. On the other hand, intracellular organelles and their polarized trafficking routes have emerged as important components of polarity. In this Review, we argue that rather than trying to identify the prime 'culprit', now it is time to consider all these players as a collective. We highlight that understanding the intimate coordination between the polarized cell cortex and the intracellular compass that is defined by organelle positioning is essential to capture the concept of polarity. After briefly reviewing how polarity emerges from a dynamic maintenance of cellular asymmetries, we highlight how intracellular organelles and their associated trafficking routes provide diverse feedback for dynamic cell polarity maintenance. We argue that the asymmetric organelle compass is an indispensable element of the polarity network.


Assuntos
Movimento Celular/fisiologia , Polaridade Celular/fisiologia , Organelas/metabolismo , Animais , Movimento Celular/genética , Polaridade Celular/genética , Humanos , Modelos Biológicos , Transdução de Sinais/genética , Transdução de Sinais/fisiologia
4.
PLoS Genet ; 14(10): e1007676, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30365533

RESUMO

Morphogen gradients provide concentration-dependent positional information along polarity axes. Although the dynamics of the establishment of these gradients is well described, precision and noise in the downstream activation processes remain elusive. A simple paradigm to address these questions is the Bicoid morphogen gradient that elicits a rapid step-like transcriptional response in young fruit fly embryos. Focusing on the expression of the major Bicoid target, hunchback (hb), at the onset of zygotic transcription, we used the MS2-MCP approach which combines fluorescent labeling of nascent mRNA with live imaging at high spatial and temporal resolution. Removing 36 putative Zelda binding sites unexpectedly present in the original MS2 reporter, we show that the 750 bp of the hb promoter are sufficient to recapitulate endogenous expression at the onset of zygotic transcription. After each mitosis, in the anterior, expression is turned on to rapidly reach a plateau with all nuclei expressing the reporter. Consistent with a Bicoid dose-dependent activation process, the time period required to reach the plateau increases with the distance to the anterior pole. Despite the challenge imposed by frequent mitoses and high nuclei-to-nuclei variability in transcription kinetics, it only takes 3 minutes at each interphase for the MS2 reporter loci to distinguish subtle differences in Bicoid concentration and establish a steadily positioned and steep (Hill coefficient ~ 7) expression boundary. Modeling based on the cooperativity between the 6 known Bicoid binding sites in the hb promoter region, assuming rate limiting concentrations of the Bicoid transcription factor at the boundary, is able to capture the observed dynamics of pattern establishment but not the steepness of the boundary. This suggests that a simple model based only on the cooperative binding of Bicoid is not sufficient to describe the spatiotemporal dynamics of early hb expression.


Assuntos
Drosophila melanogaster/embriologia , Proteínas de Homeodomínio/fisiologia , Morfogênese/fisiologia , Transativadores/fisiologia , Animais , Sítios de Ligação/genética , Padronização Corporal/genética , Proteínas de Ligação a DNA/genética , Proteínas de Drosophila/genética , Drosophila melanogaster/genética , Embrião não Mamífero/metabolismo , Regulação da Expressão Gênica no Desenvolvimento/genética , Proteínas de Homeodomínio/genética , Imagem Óptica/métodos , Regiões Promotoras Genéticas/genética , Transativadores/genética , Fatores de Transcrição/genética , Zigoto/metabolismo
5.
Int J Mol Sci ; 21(18)2020 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-32911745

RESUMO

The remote actuation of cellular processes such as migration or neuronal outgrowth is a challenge for future therapeutic applications in regenerative medicine. Among the different methods that have been proposed, the use of magnetic nanoparticles appears to be promising, since magnetic fields can act at a distance without interactions with the surrounding biological system. To control biological processes at a subcellular spatial resolution, magnetic nanoparticles can be used either to induce biochemical reactions locally or to apply forces on different elements of the cell. Here, we show that cell migration and neurite outgrowth can be directed by the forces produced by a switchable parallelized array of micro-magnetic pillars, following the passive uptake of nanoparticles. Using live cell imaging, we first demonstrate that adherent cell migration can be biased toward magnetic pillars and that cells can be reversibly trapped onto these pillars. Second, using differentiated neuronal cells we were able to induce events of neurite outgrowth in the direction of the pillars without impending cell viability. Our results show that the range of forces applied needs to be adapted precisely to the cellular process under consideration. We propose that cellular actuation is the result of the force on the plasma membrane caused by magnetically filled endo-compartments, which exert a pulling force on the cell periphery.


Assuntos
Movimento Celular/efeitos dos fármacos , Magnetismo/métodos , Nanopartículas de Magnetita/uso terapêutico , Espaço Intracelular/fisiologia , Campos Magnéticos , Nanopartículas de Magnetita/análise , Fenômenos Mecânicos , Crescimento Neuronal/efeitos dos fármacos , Fenômenos Físicos , Medicina Regenerativa/métodos
6.
Nat Mater ; 17(8): 740-746, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29967464

RESUMO

The diffusivity of macromolecules in the cytoplasm of eukaryotic cells varies over orders of magnitude and dictates the kinetics of cellular processes. However, a general description that associates the Brownian or anomalous nature of intracellular diffusion to the architectural and biochemical properties of the cytoplasm has not been achieved. Here we measure the mobility of individual fluorescent nanoparticles in living mammalian cells to obtain a comprehensive analysis of cytoplasmic diffusion. We identify a correlation between tracer size, its biochemical nature and its mobility. Inert particles with size equal or below 50 nm behave as Brownian particles diffusing in a medium of low viscosity with negligible effects of molecular crowding. Increasing the strength of non-specific interactions of the nanoparticles within the cytoplasm gradually reduces their mobility and leads to subdiffusive behaviour. These experimental observations and the transition from Brownian to subdiffusive motion can be captured in a minimal phenomenological model.


Assuntos
Citosol/metabolismo , Nanopartículas/química , Difusão , Células HeLa , Humanos , Tamanho da Partícula , Pontos Quânticos/química , Pontos Quânticos/metabolismo
7.
Nat Mater ; 17(11): 1048, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30232394

RESUMO

In the version of this Article originally published, Supplementary Videos 3-5 were incorrectly labelled; 3 should have been 5, 4 should have been 3 and 5 should have been 4. This has now been corrected.

8.
PLoS Comput Biol ; 14(10): e1006513, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30307984

RESUMO

Fly development amazes us by the precision and reproducibility of gene expression, especially since the initial expression patterns are established during very short nuclear cycles. Recent live imaging of hunchback promoter dynamics shows a stable steep binary expression pattern established within the three minute interphase of nuclear cycle 11. Considering expression models of different complexity, we explore the trade-off between the ability of a regulatory system to produce a steep boundary and minimize expression variability between different nuclei. We show how a limited readout time imposed by short developmental cycles affects the gene's ability to read positional information along the embryo's anterior posterior axis and express reliably. Comparing our theoretical results to real-time monitoring of the hunchback transcription dynamics in live flies, we discuss possible regulatory strategies, suggesting an important role for additional binding sites, gradients or non-equilibrium binding and modified transcription factor search strategies.


Assuntos
Proteínas de Ligação a DNA , Proteínas de Drosophila , Drosophila melanogaster , Regulação da Expressão Gênica no Desenvolvimento/genética , Modelos Genéticos , Fatores de Transcrição , Animais , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/genética , Drosophila melanogaster/crescimento & desenvolvimento , Proteínas de Homeodomínio/genética , Proteínas de Homeodomínio/metabolismo , Larva , Transativadores/genética , Transativadores/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
9.
Nano Lett ; 18(12): 7635-7641, 2018 12 12.
Artigo em Inglês | MEDLINE | ID: mdl-30380877

RESUMO

The mechanical manipulation of magnetic nanoparticles is a powerful approach to probing and actuating biological processes in living systems. Implementing this technique in high-throughput assays can be achieved using biocompatible micromagnet arrays. However, the magnetic properties of these arrays are usually indirectly inferred from simulations or Stokes drag measurements, leaving unresolved questions about the actual profile of the magnetic fields at the micrometer scale and the exact magnetic forces that are applied. Here, we exploit the magnetic field sensitivity of nitrogen-vacancy color centers in diamond to map the 3D stray magnetic field produced by a single soft ferromagnetic microstructure. By combining this wide-field optical magnetometry technique with magneto-optic Kerr effect microscopy, we fully analyze the properties of the micromagnets, including their magnetization saturation and their size-dependent magnetic susceptibility. We further show that the high magnetic field gradients produced by the micromagnets, greater than 104 T·m-1 under an applied magnetic field of about 100 mT, enables the manipulation of magnetic nanoparticles smaller than 10 nm inside living cells. This work paves the way for quantitative and parallelized experiments in magnetogenetics and magnetomechanics in cell biology.


Assuntos
Materiais Biocompatíveis/química , Diamante/química , Magnetometria/métodos , Imãs/química , Fenômenos Biomecânicos , Desenho de Equipamento , Células HeLa , Humanos , Lasers , Campos Magnéticos , Magnetometria/instrumentação , Microscopia/instrumentação , Microscopia/métodos , Nanopartículas/química , Nitrogênio/química , Dispositivos Ópticos , Tamanho da Partícula
10.
Int J Mol Sci ; 19(12)2018 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-30558189

RESUMO

Cellular activation of RAS GTPases into the GTP-binding "ON" state is a key switch for regulating brain functions. Molecular protein structural elements of rat sarcoma (RAS) and RAS homolog protein enriched in brain (RHEB) GTPases involved in this switch are discussed including their subcellular membrane localization for triggering specific signaling pathways resulting in regulation of synaptic connectivity, axonal growth, differentiation, migration, cytoskeletal dynamics, neural protection, and apoptosis. A beneficial role of neuronal H-RAS activity is suggested from cellular and animal models of neurodegenerative diseases. Recent experiments on optogenetic regulation offer insights into the spatiotemporal aspects controlling RAS/mitogen activated protein kinase (MAPK) or phosphoinositide-3 kinase (PI3K) pathways. As optogenetic manipulation of cellular signaling in deep brain regions critically requires penetration of light through large distances of absorbing tissue, we discuss magnetic guidance of re-growing axons as a complementary approach. In Parkinson's disease, dopaminergic neuronal cell bodies degenerate in the substantia nigra. Current human trials of stem cell-derived dopaminergic neurons must take into account the inability of neuronal axons navigating over a large distance from the grafted site into striatal target regions. Grafting dopaminergic precursor neurons directly into the degenerating substantia nigra is discussed as a novel concept aiming to guide axonal growth by activating GTPase signaling through protein-functionalized intracellular magnetic nanoparticles responding to external magnets.


Assuntos
Encéfalo/fisiologia , Proteína Enriquecida em Homólogo de Ras do Encéfalo/metabolismo , Proteínas ras/metabolismo , Animais , Diferenciação Celular , Movimento Celular , Humanos , Neurogênese , Optogenética , Transdução de Sinais
11.
PLoS Comput Biol ; 12(12): e1005256, 2016 12.
Artigo em Inglês | MEDLINE | ID: mdl-27942043

RESUMO

The simultaneous expression of the hunchback gene in the numerous nuclei of the developing fly embryo gives us a unique opportunity to study how transcription is regulated in living organisms. A recently developed MS2-MCP technique for imaging nascent messenger RNA in living Drosophila embryos allows us to quantify the dynamics of the developmental transcription process. The initial measurement of the morphogens by the hunchback promoter takes place during very short cell cycles, not only giving each nucleus little time for a precise readout, but also resulting in short time traces of transcription. Additionally, the relationship between the measured signal and the promoter state depends on the molecular design of the reporting probe. We develop an analysis approach based on tailor made autocorrelation functions that overcomes the short trace problems and quantifies the dynamics of transcription initiation. Based on live imaging data, we identify signatures of bursty transcription initiation from the hunchback promoter. We show that the precision of the expression of the hunchback gene to measure its position along the anterior-posterior axis is low both at the boundary and in the anterior even at cycle 13, suggesting additional post-transcriptional averaging mechanisms to provide the precision observed in fixed embryos.


Assuntos
Proteínas de Ligação a DNA/genética , Proteínas de Drosophila/genética , Drosophila/genética , Modelos Genéticos , Fatores de Transcrição/genética , Transcrição Gênica/genética , Animais , Ciclo Celular/genética , Biologia Computacional , Proteínas de Ligação a DNA/metabolismo , Proteínas de Drosophila/metabolismo , Embrião não Mamífero , Desenvolvimento Embrionário/genética , Fatores de Tempo , Fatores de Transcrição/metabolismo
12.
Nano Lett ; 15(5): 3487-94, 2015 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-25895433

RESUMO

Tools for controlling the spatial organization of proteins are a major prerequisite for deciphering mechanisms governing the dynamic architecture of living cells. Here, we have developed a generic approach for inducing and maintaining protein gradients inside living cells by means of biofunctionalized magnetic nanoparticles (MNPs). For this purpose, we tailored the size and surface properties of MNPs in order to ensure unhindered mobility in the cytosol. These MNPs with a core diameter below 50 nm could be rapidly relocalized in living cells by exploiting biased diffusion at weak magnetic forces in the femto-Newton range. In combination with MNP surface functionalization for specific in situ capturing of target proteins as well as efficient delivery into the cytosplasm, we here present a comprehensive technology for controlling intracellular protein gradients with a temporal resolution of a few tens of seconds.


Assuntos
Citosol/efeitos dos fármacos , Nanopartículas de Magnetita/química , Proteínas/química , Linhagem Celular , Citosol/química , Humanos , Nanopartículas de Magnetita/administração & dosagem , Tamanho da Partícula , Proteínas/administração & dosagem , Propriedades de Superfície
13.
Biophys J ; 109(9): 1785-97, 2015 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-26536256

RESUMO

Recently developed optogenetic methods promise to revolutionize cell biology by allowing signaling perturbations to be controlled in space and time with light. However, a quantitative analysis of the relationship between a custom-defined illumination pattern and the resulting signaling perturbation is lacking. Here, we characterize the biophysical processes governing the localized recruitment of the Cryptochrome CRY2 to its membrane-anchored CIBN partner. We develop a quantitative framework and present simple procedures that enable predictive manipulation of protein distributions on the plasma membrane with a spatial resolution of 5 µm. We show that protein gradients of desired levels can be established in a few tens of seconds and then steadily maintained. These protein gradients can be entirely relocalized in a few minutes. We apply our approach to the control of the Cdc42 Rho GTPase activity. By inducing strong localized signaling perturbation, we are able to monitor the initiation of cell polarity and migration with a remarkable reproducibility despite cell-to-cell variability.


Assuntos
Membrana Celular/metabolismo , Criptocromos/metabolismo , Optogenética/métodos , Proteína cdc42 de Ligação ao GTP/metabolismo , Animais , Difusão , Recuperação de Fluorescência Após Fotodegradação , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Células HeLa , Humanos , Luz , Proteínas Luminescentes/genética , Proteínas Luminescentes/metabolismo , Camundongos , Células NIH 3T3 , Proteína cdc42 de Ligação ao GTP/genética , Proteína Vermelha Fluorescente
14.
Development ; 137(14): 2253-64, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20570935

RESUMO

Morphogen gradients provide embryonic tissues with positional information by inducing target genes at different concentration thresholds and thus at different positions. The Bicoid morphogen gradient in Drosophila melanogaster embryos has recently been analysed quantitatively, yet how it forms remains a matter of controversy. Several biophysical models that rely on production, diffusion and degradation have been formulated to account for the observed dynamics of the Bicoid gradient, but no one model can account for all its characteristics. Here, we discuss how existing data on this gradient fit the various proposed models and what aspects of gradient formation these models fail to explain. We suggest that knowing a few additional parameters, such as the lifetime of Bicoid, would help to identify and develop better models of Bicoid gradient formation.


Assuntos
Drosophila melanogaster/embriologia , Modelos Biológicos , Animais , Difusão , Drosophila melanogaster/genética , Drosophila melanogaster/metabolismo
15.
FEBS Lett ; 597(6): 836-849, 2023 03.
Artigo em Inglês | MEDLINE | ID: mdl-36658753

RESUMO

RhoGTPases are well known for being controllers of cell cytoskeleton and share common features in the way they act and are controlled. These include their switch from GDP to GTP states, their regulations by different guanine exchange factors (GEFs), GTPase-activating proteins and guanosine dissociation inhibitors (GDIs), and their similar structure of active sites/membrane anchors. These very similar features often lead to the common consideration that the differences in their biological effects mainly arise from the different types of regulators and specific effectors associated with each GTPase. Focusing on data obtained through biosensors, live cell microscopy and recent optogenetic approaches, we highlight in this review that the regulation of RhoA appears to depart from Cdc42 and Rac1 modes of regulation through its enhanced lability at the plasma membrane. RhoA presents a high dynamic turnover at the membrane that is regulated not only by GDIs but also by GEFs, effectors and a possible soluble conformational state. This peculiarity of RhoA regulation may be important for the specificities of its functions, such as the existence of activity waves or its putative dual role in the initiation of protrusions and contractions.


Assuntos
Fatores de Troca do Nucleotídeo Guanina , Proteína rhoA de Ligação ao GTP , Proteína rhoA de Ligação ao GTP/genética , Proteína rhoA de Ligação ao GTP/metabolismo , Fatores de Troca do Nucleotídeo Guanina/metabolismo , Proteínas Ativadoras de GTPase/metabolismo , Inibidores de Dissociação do Nucleotídeo Guanina/química , Inibidores de Dissociação do Nucleotídeo Guanina/metabolismo
16.
Curr Opin Genet Dev ; 18(4): 342-7, 2008 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-18602472

RESUMO

The first direct studies of morphogen gradients were done in the end of 1980s, in the early Drosophila embryo, which is patterned under the action of four maternally determined morphogens. Since the early studies of maternal morphogens were done with fixed embryos, they were viewed as relatively static signals. Several recent studies analyze dynamics of the anterior, dorsoventral, and terminal patterning signals. The results of these quantitative studies provide critical tests of classical models and reveal new modes of morphogen regulation and readout in one of the most extensively studied patterning systems.


Assuntos
Padronização Corporal/fisiologia , Drosophila/embriologia , Proteínas de Insetos/metabolismo , Morfogênese/fisiologia , Animais , Padronização Corporal/genética , Drosophila/genética , Drosophila/metabolismo , Embrião não Mamífero , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , MAP Quinases Reguladas por Sinal Extracelular/fisiologia , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Proteínas de Insetos/farmacologia , Modelos Biológicos , Morfogênese/genética , Transporte Proteico , Transdução de Sinais/fisiologia
17.
Proc Natl Acad Sci U S A ; 106(4): 1087-92, 2009 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-19147842

RESUMO

Biological networks frequently use cascades, generally defined as chain-like arrangements of similar modules. Spatially lumped cascades can serve as noise filters, time-delay, or thresholding elements. The operation and functional capabilities of spatially distributed cascades are much less understood. Motivated by studies of pattern formation in the early Drosophila embryo, we analyze cascades of 2-state reaction-diffusion systems. At each stage within such as a cascade, a diffusible particle is reversibly bound by immobile traps and can be annihilated in both mobile and immobile states. When trapped, these particles drive the next stage by converting mobile particles of a different type from a passive to active form. The cascade initiated by injection of mobile particles into the first stage. We derive analytical expressions for the steady-state concentration profiles of mobile and immobile particles and analyze how the output of a cascade is controlled by properties of the constituent stages.


Assuntos
Drosophila melanogaster/metabolismo , Transdução de Sinais , Animais , Difusão , Drosophila melanogaster/enzimologia , Sistema de Sinalização das MAP Quinases , Fosforilação
18.
Elife ; 112022 03 18.
Artigo em Inglês | MEDLINE | ID: mdl-35302488

RESUMO

Migrating cells present a variety of paths, from random to highly directional ones. While random movement can be explained by basal intrinsic activity, persistent movement requires stable polarization. Here, we quantitatively address emergence of persistent migration in (hTERT)-immortalizedRPE1 (retinal pigment epithelial) cells over long timescales. By live cell imaging and dynamic micropatterning, we demonstrate that the Nucleus-Golgi axis aligns with direction of migration leading to efficient cell movement. We show that polarized trafficking is directed toward protrusions with a 20-min delay, and that migration becomes random after disrupting internal cell organization. Eventually, we prove that localized optogenetic Cdc42 activation orients the Nucleus-Golgi axis. Our work suggests that polarized trafficking stabilizes the protrusive activity of the cell, while protrusive activity orients this polarity axis, leading to persistent cell migration. Using a minimal physical model, we show that this feedback is sufficient to recapitulate the quantitative properties of cell migration in the timescale of hours.


Assuntos
Polaridade Celular , Complexo de Golgi , Movimento Celular/fisiologia , Polaridade Celular/fisiologia
19.
Elife ; 112022 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-35363606

RESUMO

For over 40 years, the Bicoid-hunchback (Bcd-hb) system in the fruit fly embryo has been used as a model to study how positional information in morphogen concentration gradients is robustly translated into step-like responses. A body of quantitative comparisons between theory and experiment have since questioned the initial paradigm that the sharp hb transcription pattern emerges solely from diffusive biochemical interactions between the Bicoid transcription factor and the gene promoter region. Several alternative mechanisms have been proposed, such as additional sources of positional information, positive feedback from Hb proteins or out-of-equilibrium transcription activation. By using the MS2-MCP RNA-tagging system and analysing in real time, the transcription dynamics of synthetic reporters for Bicoid and/or its two partners Zelda and Hunchback, we show that all the early hb expression pattern features and temporal dynamics are compatible with an equilibrium model with a short decay length Bicoid activity gradient as a sole source of positional information. Meanwhile, Bicoid's partners speed-up the process by different means: Zelda lowers the Bicoid concentration threshold required for transcriptional activation while Hunchback reduces burstiness and increases the polymerase firing rate.


Assuntos
Proteínas de Drosophila , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Homeodomínio/metabolismo , Regiões Promotoras Genéticas , Transativadores/metabolismo
20.
Sci Adv ; 8(39): eabp8416, 2022 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-36179021

RESUMO

Cell migration is essential to living organisms and deregulated in cancer. Single cell's migration ranges from traction-dependent mesenchymal motility to contractility-driven propulsive amoeboid locomotion, but collective cell migration has only been described as a focal adhesion-dependent and traction-dependent process. Here, we show that cancer cell clusters, from patients and cell lines, migrate without focal adhesions when confined into nonadhesive microfabricated channels. Clusters coordinate and behave like giant super cells, mobilizing their actomyosin contractility at the rear to power their migration. This polarized cortex does not sustain persistent retrograde flows, of cells or actin, like in the other modes of migration but rather harnesses fluctuating cell deformations, or jiggling. Theoretical physical modeling shows this is sufficient to create a gradient of friction forces and trigger directed cluster motion. This collective amoeboid mode of migration could foster metastatic spread by enabling cells to cross a wide spectrum of environments.

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