RESUMO
(1) Background: The validation of biological antigens is the study's utmost goal in biomedical applications. We evaluated three different probes with single and multiple epitopes through electrochemical detection of specific IgG in serum for human strongyloidiasis diagnosis. (2) Methods: Screen-printed gold electrodes were used and probes consisting of two single-epitope synthetic peptides (D3 and C10) with different sequences, and a multi-epitope antigen [detergent phase (DP)-hydrophobic membrane proteins]. Human serum samples from three populations were used: Strongyloides stercoralis positive, positive for other parasitic infections and negative controls. To test the immobilization of probes onto a screen-printed gold electrode and the serum IgG detection, electrochemical analyses were carried out through differential pulse voltammetry (DPV) and the electrode surface analyses were recorded using atomic force microscopy. (3) Results: The electrochemical response in screen-printed gold electrodes of peptides D3 and C10 when using positive serum was significantly higher than that when using the DP. Our sensor improved sensitivity to detect strongyloidiasis. (4) Conclusions: Probes' sequences are critical factors for differential electrochemical responses, and the D3 peptide presented the best electrochemical performance for strongyloidiasis detection, and may efficiently substitute whole antigen extracts from parasites for strongyloidiasis diagnosis in electrochemical immunosensors.
Assuntos
Técnicas Biossensoriais , Estrongiloidíase , Animais , Técnicas Eletroquímicas , Eletrodos , Ouro , Humanos , ImunoensaioRESUMO
Human neurocysticercosis (NCC) is a worldwide neglected disease caused by Taenia solium metacestode and responsible for various complications and neurological disorders. This study aimed to evaluate the use of specific immunoglobulin Y (IgY) produced by laying hens immunized with a hydrophobic fraction of Taenia crassiceps metacestodes (hFTc) in NCC diagnosis. Egg yolk IgY antibodies were fractionated, purified and characterized. Enzyme-linked immunosorbent assay (ELISA) was carried out to evaluate the production kinetics and avidity maturation of anti-hFTc IgY antibodies throughout the IgY obtention process. Antigen recognition tests were carried out by Western blotting and immunofluorescence antibody test using purified and specific anti-hFTc IgY antibodies for detection of parasitic antigens of T. crassiceps and T. solium metacestodes. Sandwich ELISA was performed to detect circulating immune complexes formed by IgG and parasitic antigens in human sera. The results showed high diagnostic values (93.2% sensitivity and 94.3% specificity) for immune complexes detection in human sera with confirmed NCC. In conclusion, specific IgY antibodies produced from immunized hens with hFTc antigens were efficient to detect T. solium immune complexes in human sera, being an innovative and potential tool for NCC immunodiagnosis.
Assuntos
Antígenos de Helmintos/imunologia , Imunoglobulinas/sangue , Testes Imunológicos/métodos , Neurocisticercose/parasitologia , Taenia/isolamento & purificação , Animais , Afinidade de Anticorpos , Galinhas , Feminino , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Neurocisticercose/imunologia , Óvulo , Taenia/imunologiaRESUMO
Parasite infections affect billions of people and their domesticated animals worldwide, and remain as a significant cause of morbidity and mortality, but such diseases are still neglected in endemic countries. Therapeutic interventions consisted mostly of drugs, which are highly toxic and may lead to resistance. The immunopathology of parasites is very complex due to their multistage life cycles and long lifetime involving several hosts, leading many times to chronic infections and sometimes to death, by compromising nutritional status, affecting cognitive processes, and inducing severe tissue reactions. Vaccination is a challenge, and immunotherapy is completely disregarded because of their complex interactions with hosts and vectors. This review will bring concepts of immunological aspects for some important parasitic infections, and present the most recent phage display-derived antibodies or peptidomimetics for parasite targets. This chapter will also discuss the future perspectives of such potential anti-infective immunobiologicals for parasitic diseases.
Assuntos
Anticorpos/uso terapêutico , Antiparasitários/uso terapêutico , Técnicas de Visualização da Superfície Celular , Parasitos/efeitos dos fármacos , Doenças Parasitárias/tratamento farmacológico , Biblioteca de Peptídeos , Animais , Anticorpos/efeitos adversos , Anticorpos/imunologia , Antiparasitários/efeitos adversos , Antiparasitários/imunologia , Interações Hospedeiro-Parasita , Humanos , Parasitos/imunologia , Parasitos/patogenicidade , Doenças Parasitárias/imunologia , Doenças Parasitárias/parasitologiaRESUMO
Definitive diagnosis of strongyloidiasis in humans is typically achieved by detection of larvae in fecal samples. However, limitations on sensitivity of parasitological methods emphasize the need for more robust diagnostic methods. The aim of this study was to compare the diagnostic value of three methods: eggs per gram of feces (EPG), coproantigen detection by enzyme linked immunosorbent assay (ELISA), and DNA detection by conventional polymerase chain reaction (PCR). The assays were performed at 0 and 5, 8, 13, 21 and 39 days post-infection (dpi) using fecal samples from experimentally infected immunocompetent and immunosuppressed rats. In immunocompetent rats, eggs were detected in feces on days 5, 8 and 13 dpi; coproantigen detection and PCR amplification were successful at all post-infection time points (5, 8, 13, 21 and 39 dpi). In immunosuppressed rats, eggs were detected at 5, 8, 13 and 21; coproantigen detection and PCR amplification were successful at all post-infection time points. In conclusion, these results suggest that coproantigen detection and PCR may be more sensitive alternatives to traditional methods such as EPG for diagnosis of Strongyloides venezuelensis infection.
Assuntos
Antígenos de Helmintos/isolamento & purificação , DNA de Helmintos/isolamento & purificação , Fezes/parasitologia , Strongyloides/isolamento & purificação , Estrongiloidíase/diagnóstico , Animais , Sequência de Bases , DNA de Helmintos/química , Ensaio de Imunoadsorção Enzimática , Fezes/química , Imunocompetência , Hospedeiro Imunocomprometido , Masculino , Contagem de Ovos de Parasitas , Reação em Cadeia da Polimerase , Ratos , Ratos Wistar , Alinhamento de Sequência , Strongyloides/genética , Strongyloides/imunologia , Estrongiloidíase/parasitologiaRESUMO
Neurocysticercosis (NC) is one of the most important diseases caused by parasites affecting the central nervous system. We fractionated by ion-exchange chromatography using diethylaminoethyl (DEAE)-sepharose resin the total saline extract (S) from Taenia solium metacestodes and evaluated obtained fractions (DEAE S1 and DEAE S2) by enzyme-linked immunosorbent assay (ELISA, n = 123) and immunoblotting (IB, n = 22) to detect human NC in serum. Diagnostic parameters were established by ROC and TG ROC curves for ELISA tests. IB was qualitatively analyzed. S and DEAE S1 presented sensitivity of 87. 5% and DEAE S2 90%. The best specificity was observed for DEAE S2 (90.4%). In IB, using DEAE S2 samples from NC patients presented bands of 20-25, 43-45, 55-50, 60-66, 82, 89, and 140 kDa. The great diagnostic parameters reached by DEAE S2 suggest the potential applicability of this fraction in NC immunodiagnosis.
Assuntos
Anticorpos Anti-Helmínticos/sangue , Antígenos de Helmintos/isolamento & purificação , Etanolaminas/química , Imunoglobulina G/sangue , Neurocisticercose/diagnóstico , Taenia solium/imunologia , Animais , Antígenos de Helmintos/imunologia , Fracionamento Químico , Cromatografia por Troca Iônica , Ensaio de Imunoadsorção Enzimática , Humanos , Immunoblotting , Neurocisticercose/sangue , Neurocisticercose/imunologia , Sensibilidade e Especificidade , Testes Sorológicos , Suínos , Taenia solium/isolamento & purificaçãoRESUMO
In order to establish an antigen, antibody and immune complex detection by enzyme-linked immunosorbent assay (ELISA) in serum samples, normal or immunocompromised Wistar rats experimentally infected with Strongyloides venezuelensis were used. The microtitre plates were coated with IgG anti-S. venezuelensis for antigen and immune complex detection and with alkaline parasite extract for antibody detection. Analysis revealed at least 12.5 µg/mL of S. venezuelensis specific antigens in serum samples. Assay for antigen detection was not a good approach for evaluating infection in normal or immunocompromised rats. In normal rats IgG specific for S. venezuelensis was preferentially detected during the first 13 days post-infection (p.i.) and immune complex detection was significantly reduced in 21 p.i. day. On the other hand, in immunocompromised rats, IgG and immune complex were detected during the entire kinetic (5, 8, 13 and 21 p.i). These results suggest that immune complex screening seems to be an alternative for early strongyloidiasis diagnosis in immunocompromised individuals.
Assuntos
Anticorpos Anti-Helmínticos/sangue , Complexo Antígeno-Anticorpo/sangue , Antígenos de Helmintos/sangue , Strongyloides/imunologia , Estrongiloidíase/imunologia , Animais , Fezes/parasitologia , Hospedeiro Imunocomprometido , Imunoglobulina G/sangue , Masculino , Contagem de Ovos de Parasitas , Ratos , Ratos Wistar , Estrongiloidíase/sangue , Estrongiloidíase/diagnósticoRESUMO
This study was performed with the objective of developing innovative procedures for the diagnosis of strongyloidiasis. Enzyme-linked immunosorbent assay (ELISA) was conducted to detect coproantigen in the faecal samples of normal and of immunosuppressed rats using an anti-L3 polyclonal antibody produced in rabbits. Analysis revealed the kinetics of egg shedding in the non-immunosuppressed and immunosuppressed rats infected with S. venezuelensis. Further analysis verified the ability of the immune serum to detect L3 antigens in faecal samples from infected animals. The number of eggs recovered in the faeces at 8 days p.i was significantly higher for both groups. Immunosuppressed animals eliminated increased quantities of eggs. The immune serum was able to detect 0.39 microg/ml of L3 antigens. The antigen recognition in the immunosuppressed group was anticipated on the 8th day p.i. In conclusion, these results may represent a first step in the development of a rapid coproantigen detection kit for strongyloidiasis.
Assuntos
Antígenos de Helmintos/análise , Fezes/parasitologia , Soros Imunes , Strongyloides/isolamento & purificação , Estrongiloidíase/diagnóstico , Animais , Anticorpos Anti-Helmínticos/imunologia , Antígenos de Helmintos/imunologia , Modelos Animais de Doenças , Ensaio de Imunoadsorção Enzimática , Soros Imunes/imunologia , Terapia de Imunossupressão , Cinética , Masculino , Contagem de Ovos de Parasitas , Coelhos , Ratos , Ratos Wistar , Strongyloides/imunologia , Estrongiloidíase/imunologiaRESUMO
Neurocysticercosis (NC) is the most important neurological disease of parasitic origin in humans. IgA and IgG detection in serum from neurocysticercosis patients was tested using some antigenic preparations of total saline extract from Taenia saginata: detergent (D) and aqueous (A) phases extracted with Triton X-114 and the jacalin bound (JBF) and unbound fractions (JUF) obtained by affinity chromatography using jacalin column. Samples were obtained from 45 patients with definitive NC, who were subdivided into active-NC group and inactive-NC group; 35 patients with other parasitoses; and 30 apparently healthy individuals. Sensitivity and specificity were calculated. Specificity to detect IgA and IgG for D phase, respectively, were 89.8% and 86.9% and for IgG detection 91.3% and 76.8% when using D phase and JUF, respectively. D phase and JBF proved to be specific and efficient and could be efficiently utilized as an alternative antigen for IgA detection in NC, with comparable results with IgG.
Assuntos
Anticorpos Anti-Helmínticos/sangue , Antígenos de Helmintos , Antígenos Heterófilos , Imunoglobulina A/sangue , Neurocisticercose/diagnóstico , Parasitologia/métodos , Taenia saginata/química , Animais , Antígenos de Helmintos/isolamento & purificação , Antígenos Heterófilos/isolamento & purificação , Fracionamento Celular , Cromatografia de Afinidade , Humanos , Imunoglobulina G/sangue , Neurocisticercose/imunologia , Sensibilidade e Especificidade , Taenia saginata/imunologiaRESUMO
Strongyloides venezuelensis is an important alternative source of antigen for the serologic diagnosis of human strongyloidiasis. Proteomics techniques applied to the analysis of the protein content of infective third stage larvae (iL3) of S. venezuelensis provide a powerful tool for the discovery of new candidates for immunodiagnosis. This study presents an overview of the protein iL3 S. venezuelensis focusing on the diagnosis of strongyloidiasis. A total of 877 proteins were identified by shotgun proteomics. Many of these proteins are involved in different cellular processes, metabolic as well as structural maintenance. Our results point to a catalog of possible diagnostic targets for human strongyloidiasis and highlight the need for evaluation of uncharacterized proteins, especially the proteins within the CAP domain, transthyretin, and BTPI inhibitor domains, as a repertoire as yet unexplored in the context of strongyloidiasis diagnostic markers. We believe that the protein profile presented in this shotgun analysis extends our understanding of the protein composition within the Strongyloides genus, opening up new perspectives for research on biomarkers that may help with the diagnosis of human strongyloidiasis. Data are available via ProteomeXchange with identifier PXD013703.
Assuntos
Biomarcadores/metabolismo , Larva/metabolismo , Proteoma , Strongyloides/metabolismo , Estrongiloidíase/diagnóstico , Animais , Catepsinas/metabolismo , Galectinas/metabolismo , Interações Hospedeiro-Parasita , Humanos , Testes Imunológicos , Metaloproteases/metabolismo , Patologia Molecular , ProteômicaRESUMO
BACKGROUND: Neurocysticercosis (NCC) is a neglected tropical disease and its diagnosis is still a challenge due to non-specific manifestations. Neuroimaging techniques are used in the diagnosis of NCC, however, due to the high cost of these methods and the advantages presented in the use of immunological tests, such as ease of performance and satisfactory results, immunoassays are commonly used to detect antibodies against Taenia sp. antigens. The aim of the present study was to produce, characterize and apply specific polyclonal immunoglobulin Y (IgY) anti-Taenia crassiceps extracted from egg yolk of hens immunized with T. crassiceps metacestodes. METHODS: Indirect enzyme-linked immunosorbent assay (ELISA), avidity ELISA, immunoblotting and indirect immunofluorescence tests were performed for characterization of IgY antibodies. Diagnostic performance was verified by ELISA for immune complex detection testing 90 serum samples. RESULTS: Values of sensitivity, specificity, positive and negative likelihood ratios (LR+/LR-) and area under the curve (AUC) were calculated and presented the following results: sensitivity 83.3%, specificity 96.7%, AUC 0.966, LR+ 25.0 and LR- 0.17. CONCLUSIONS: Results of this pioneering and innovative study demonstrate that anti-T. crassiceps IgY antibodies present potential applicability and can be used as an efficient tool in human NCC serodiagnosis.
Assuntos
Neurocisticercose , Animais , Anticorpos Anti-Helmínticos , Complexo Antígeno-Anticorpo , Antígenos de Helmintos , Galinhas , Gema de Ovo , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imunoglobulinas , Neurocisticercose/diagnóstico , Sensibilidade e EspecificidadeRESUMO
Strongyloides stercoralis is an intestinal nematode capable of chronic, persistent infection and hyperinfection of the host; this can lead to dissemination, mainly in immunosuppressive states, in which the infection can become severe and result in the death of the host. In this study, we investigated the immune response against Strongyloides venezuelensis infection in major histocompatibility complex (MHC) class I or class II deficient mice. We found that MHC II(-/-) animals were more susceptible to S. venezuelensis infection as a result of the presence of an elevated number of eggs in the faeces and a delay in the elimination of adult worms compared with wild-type (WT) and MHC I(-/-) mice. Histopathological analysis revealed that MHC II(-/-) mice had a mild inflammatory infiltration in the small intestine with a reduction in tissue eosinophilia. These mice also presented a significantly lower frequency of eosinophils and mononuclear cells in the blood, together with reduced T helper type 2 (Th2) cytokines in small intestine homogenates and sera compared with WT and MHC I(-/-) animals. Additionally, levels of parasite-specific immunoglobulin M (IgM), IgA, IgE, total IgG and IgG1 were also significantly reduced in the sera of MHC II(-/-) infected mice, while a non-significant increase in the level of IgG2a was found in comparison to WT or MHC I(-/-) infected mice. Together, these data demonstrate that expression of MHC class II but not class I molecules is required to induce a predominantly Th2 response and to achieve efficient control of S. venezuelensis infection in mice.
Assuntos
Citocinas/imunologia , Antígenos de Histocompatibilidade Classe II/imunologia , Antígenos de Histocompatibilidade Classe I/imunologia , Intestino Delgado/imunologia , Strongyloides , Estrongiloidíase/imunologia , Animais , Citocinas/biossíntese , Eosinófilos/imunologia , Eosinófilos/parasitologia , Fezes/parasitologia , Antígenos de Histocompatibilidade Classe I/genética , Antígenos de Histocompatibilidade Classe II/genética , Imunoglobulinas/sangue , Intestino Delgado/parasitologia , Leucócitos Mononucleares/imunologia , Leucócitos Mononucleares/parasitologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Contagem de Ovos de Parasitas , Estrongiloidíase/genética , Estrongiloidíase/parasitologia , Células Th2/imunologia , Células Th2/parasitologiaRESUMO
The aim of the present research was to test the application of Taenia saginata metacestodes as an alternative antigen for use in enzyme-linked immunosorbent assay (ELISA) and Western Blotting (WB) tests compared with the use of metacestodes antigen of Taenia solium in cerebrospinal fluid (CSF) samples. The samples were obtained from 35 patients with definitive neurocysticercosis (NCC)-group 1-and 44 patients with other neurological disorders (control)-group 2. The sensitivity and specificity of ELISA, using antigen obtained from T. solium, applied to the patients of group 1 yielded results of 100%. When the tests were conducted using T. saginata metacestodes, results were 100% and 93.2%, respectively. The 47-52-, 64-68-, and 70-kDa antigens showed high frequencies in CSF samples from patients with NCC when WB was conducted with both antigens. The results indicate that T. saginata metacestodes can be used as an alternative antigen for the diagnosis of human NCC in CSF samples.
Assuntos
Anticorpos Anti-Helmínticos/líquido cefalorraquidiano , Antígenos de Helmintos/isolamento & purificação , Neurocisticercose/diagnóstico , Taenia saginata/química , Taenia solium/química , Adolescente , Adulto , Animais , Western Blotting/métodos , Ensaio de Imunoadsorção Enzimática/métodos , Feminino , Humanos , Testes Imunológicos , Masculino , Pessoa de Meia-Idade , Sensibilidade e Especificidade , Taenia saginata/imunologia , Taenia solium/imunologia , Adulto JovemRESUMO
Due to the epidemiological problem of the neglected condition of human strongyloidiasis, rapid and effective diagnosis is extremely important, with the development of new diagnostic tools being essential to reduce infections and chronic cases. Avian immunoglobulin Y (IgY) technology is an alternative for antibody production that has high specificity and profitability. This study aimed to produce and fractionate IgY antibodies from the egg yolks of hens that were immunized with the total antigenic extracts of Strongyloides venezuelensis infectious filariform larvae (iL3) and parthenogenetic females (pF). IgY antibodies were then evaluated by their recognition of antigenic proteins, evolutive helminth forms, and serological diagnosis of human strongyloidiasis by the detection of immune complexes in serum samples. Egg yolks were fractionated to obtain IgY antibodies by thiophilic interaction chromatography. Immune complex detection in serum samples showed diagnostic values for anti-iL3 IgY and anti-pF IgY antibodies at 95.56% and 88.89% sensitivity and 95.56% and 91.11% specificity, respectively. Therefore, IgY technology is a promising tool for the detection of blood circulating Strongyloides antigens, with possible application as a serological diagnostic method.
Assuntos
Imunoglobulinas/imunologia , Testes Imunológicos/métodos , Strongyloides/imunologia , Estrongiloidíase/diagnóstico , Animais , Anticorpos Anti-Helmínticos/sangue , Antígenos de Helmintos , Galinhas , Gema de Ovo , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imunoglobulina G/sangue , Larva/imunologia , Sensibilidade e Especificidade , Testes Sorológicos , Estrongiloidíase/imunologiaRESUMO
Strongyloidiasis is a human parasitosis that is considered a public health problem. Early diagnosis of this infection is extremely important in immunocompromised patients (i.e. subjects with alcoholism). This study aimed to evaluate anti-Strongyloides immunoglobulin G (IgG) and immunoglobulin A (IgA), assess levels of circulating immune complexes (IC) and determine IgG avidity in serum samples from alcoholic and nonalcoholic individuals. A total of 140 blood samples were collected from male individuals (70 alcoholic and 70 nonalcoholic subjects). Serum was obtained and analysed by enzyme-linked immunosorbent assay for IgG, IgA, IC detection and avidity determination. Anti-Strongyloides IgG was detected in 55.7% of alcoholic subjects and 32.8% nonalcoholics, while IC levels showed frequencies of 38.6% and 17.1% in these groups, respectively. Anti-Strongyloides IgA was lower among alcoholics (4.3%) than nonalcoholics (34.3%). Spearman's correlation coefficient reported a positive correlation between IgG, IC and IgA in alcoholic individuals and no correlation in nonalcoholics. The median avidity index was higher in alcoholics (83.8%) than nonalcoholic subjects (73.2%). In conclusion, this study shows that alcoholic subjects produced specific antibodies against S. stercoralis regardless of the possible immunosuppression caused by chronic alcoholism. Considering that alcoholics are more susceptible to the severe forms of strongyloidiasis, the implementation of immunological methods as a complementary approach to parasitological diagnostics (i.e. detection of IgG, IC and antibody avidity) appears to be an alternative method for early diagnosis in these individuals.
Assuntos
Alcoólicos , Anticorpos Anti-Helmínticos/sangue , Complexo Antígeno-Anticorpo/sangue , Estrongiloidíase/diagnóstico , Estrongiloidíase/imunologia , Adulto , Animais , Afinidade de Anticorpos , Estudos Transversais , Ensaio de Imunoadsorção Enzimática , Fezes/parasitologia , Humanos , Hospedeiro Imunocomprometido , Imunoglobulina A/sangue , Imunoglobulina G/sangue , Masculino , Pessoa de Meia-Idade , Strongyloides stercoralis , Estrongiloidíase/sangueRESUMO
INTRODUCTION: Detection of Strongyloides stercoralis larvae is particularly challenging because only a small number of larvae are released into the feces, regardless of infection stage. OBJECTIVE: Our objective was to apply conventional polymerase chain reaction (PCR) to the detection of S. stercoralis DNA in feces samples to evaluate its performance in samples of patients with strongyloidiasis and compare results with those of immunodiagnosis. METHODS: Stool, serum, and saliva samples were collected from each individual (n = 48) at the clinic hospital of the State University of Londrina, Brazil, for parasitological, immunological, and molecular tests. Stool samples were processed via parasitological methods. Serum samples were used for immunoglobulin G (IgG) detection and saliva samples for IgA detection by ELISA. RESULTS: For amplification by conventional PCR, two different primers were used: species specific (101 bp) and genus specific (392 bp). The results showed that 34 (97.1%) of the 35 copro-positive individuals for S. stercoralis were positive for serum IgG and 19 (54.3%) were positive for salivary IgA. Regarding molecular analysis, both primers (species and genus specific) demonstrated positivity in 100% of the samples, which was confirmed by sequencing the positive samples. CONCLUSION: Complementary examinations of the parasitological method demonstrated excellent results in the context of the diagnosis of strongyloidiasis, especially in asymptomatic patients with irregular larval release in the feces.
Assuntos
Imunoensaio , Técnicas de Diagnóstico Molecular , Strongyloides/genética , Strongyloides/imunologia , Estrongiloidíase/diagnóstico , Estrongiloidíase/parasitologia , Animais , Ensaio de Imunoadsorção Enzimática , Humanos , Imunoglobulina A Secretora , Imunoglobulina G/imunologia , Reação em Cadeia da Polimerase , RNA Ribossômico 18S/genética , Ratos , Saliva/imunologiaRESUMO
DNA is often damaged by many environmental agents, which lead to the up-regulation of several genes involved in different repair pathways. Schistosoma mansoni has a complex life cycle, being exposed to a subset of DNA-damaging agents, such as those present in the environment and host immune response. Recently, studies showed that nucleotide excision repair (NER) is an indispensable mechanism for removing a broad spectrum of different DNA lesions. In the present report, we showed the gene expression of nucleotide excision repair factor 2 (NEF2) SmRad23 and SmRad4, in different developmental stages of S. mansoni, as well as the differential expression of these genes in S. mansoni adult worms treated with DNA-damaging agents. Furthermore, it was revealed the correlation of these genes with their orthologues in other eukaryotes. Our reports suggest that NER is an important repair pathway during the complex life cycle of S. mansoni.
Assuntos
Reparo do DNA , Proteínas de Ligação a DNA/genética , Estágios do Ciclo de Vida , Schistosoma mansoni/crescimento & desenvolvimento , Schistosoma mansoni/genética , Sequência de Aminoácidos , Animais , Ensaio Cometa/métodos , Dano ao DNA , Proteínas de Ligação a DNA/biossíntese , Regulação da Expressão Gênica no Desenvolvimento , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Complexo de Endopeptidases do Proteassoma/genética , Complexo de Endopeptidases do Proteassoma/metabolismo , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Schistosoma mansoni/metabolismo , Caramujos/parasitologiaRESUMO
To determine the frequency of Strongyloides stercoralis antibodies by means of the enzyme linked immunosorbent assay (ELISA) in Chile, in 2001-2003, 675 blood samples of patients of two psychiatric hospitals and 172 of healthy individuals (doctors, nurses and paramedicals) of these institutions, and 1,200 serum samples of blood donors of Northern region (Arica and Antofagasta), Central region (Valparaiso and Santiago) and Southern region (La Union) were collected. ELISA showed positivity of 12.1% in psychiatric hospitalized patients, none (0%) in the health personnel and 0.25% in blood donors (p < 0.05). Only in blood donors of Arica (1%) and La Union (0.5%) the ELISA test was positive suggesting that strongyloidiasis is focalized in determinate zones of the country. In Chile, human infections by S. stercoralis are endemic with very low frequency in apparently healthy individuals and high prevalence in risk groups such as the mentally ill hospitalized patients.
Assuntos
Anticorpos Anti-Helmínticos/sangue , Strongyloides stercoralis/imunologia , Estrongiloidíase/epidemiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Doadores de Sangue , Chile/epidemiologia , Ensaio de Imunoadsorção Enzimática , Feminino , Hospitais Psiquiátricos , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , Estudos Soroepidemiológicos , Estrongiloidíase/diagnósticoRESUMO
Strongyloidosis is a neglected disease that affects millions of people around the world. The cases that particularly deserve attention are those related to hyperinfection, mainly in immunocompromised patients. In this sense, there is a need to improve the serological diagnosis of this helminth. The objective of this study was therefore to produce and characterize excretory/secretory (E/S) antigens of Strongyloides venezuelensis infective larvae (L3) for use as a heterologous antigen in the diagnosis of human strongyloidosis and other parasitic infection groups. Soluble antigenic preparations were produced as total saline extract (SE), E/S in Roswell Park Memorial Institute 1640 (RPMI) and E/S in phosphate buffered saline (PBS). The three antigenic preparations showed similar protein bands. An ELISA showed that the E/S antigens were profitable, easy to use, and more sensitive and specific than SE, eliminating cross-reactivity with other parasites in serum samples. The detection of anti-Strongyloides stercoralis in the sera of patients with strongyloidosis and those with immunosuppressive conditions using S. venezuelensis L3 larvae E/S antigens was satisfactory. RPMI and PBS E/S antigens were also superior in terms of specificity than SE.
Assuntos
Anticorpos Anti-Helmínticos/sangue , Antígenos de Helmintos/imunologia , Imunoglobulina G/sangue , Strongyloides stercoralis/imunologia , Estrongiloidíase/diagnóstico , Animais , Antígenos de Helmintos/química , Antígenos de Helmintos/isolamento & purificação , Antígenos de Helmintos/metabolismo , Reações Cruzadas , Ensaio de Imunoadsorção Enzimática/métodos , Humanos , Hospedeiro Imunocomprometido , Testes Imunológicos , Sensibilidade e Especificidade , Estrongiloidíase/imunologiaRESUMO
This study aimed to evaluate the total extract of Taenia crassiceps metacestodes (TC) and its antigenic fractions obtained by Triton X-114 fractionation techniques, such as detergent (DC) and aqueous (AC), in the immunodiagnosis of human neurocysticercosis (NCC). Cerebrospinal fluid samples were divided into two groups: Group 1 (n=40), which was further divided into active (n=20) and inactive (n=20) NCC, and Group 2 (control group), which comprised 39 CSF samples from patients who had another neurological disorder, were suffering from other infectious diseases of the brain or had other parasitic infections. The total extracts and antigenic fractions were tested by enzyme-linked immunosorbent assay (ELISA) to detect human IgG anti-Taenia solium. T. crassiceps fractions (DC and AC) showed the same value of sensitivity (Se), 100%, for active and inactive NCC and a specificity (Sp) of 97.4%. The DS fraction obtained from T. solium showed 100% Se for active NCC, 95% Se for inactive NCC and a 92.3% Sp. The AS fraction obtained from T. solium showed 100% Se for both active and inactive NCC and a 94.9% Sp. There was a positive correlation between the total saline extract of T. crassiceps (TC) and T. solium (TS) and their fractions (DC, AC, DS and AS). Positive predictive value, negative predictive value, diagnostic efficiency and Youden index were calculated. In conclusion, these results demonstrated that detergent and aqueous fractions obtained from T. crassiceps metacestodes are important sources of specific antigens and are efficient for immunodiagnosis of active and inactive NCC.
Assuntos
Anticorpos Anti-Helmínticos/líquido cefalorraquidiano , Antígenos de Helmintos/imunologia , Antígenos de Helmintos/isolamento & purificação , Ensaio de Imunoadsorção Enzimática , Neurocisticercose/diagnóstico , Neurocisticercose/imunologia , Taenia/imunologia , Animais , Antígenos de Helmintos/química , Fracionamento Químico/métodos , Feminino , Humanos , Interações Hidrofóbicas e Hidrofílicas , Imunoglobulina G/líquido cefalorraquidiano , Larva/química , Larva/imunologia , Masculino , Camundongos , Neurocisticercose/líquido cefalorraquidiano , Octoxinol , Polietilenoglicóis , Valor Preditivo dos Testes , Sensibilidade e Especificidade , Cloreto de Sódio , Taenia/fisiologiaRESUMO
The purpose of this study was to determine the frequency of association between positive Strongyloides stercoralis serology and diabetes mellitus. A total of 78 diabetic patients and 42 controls were evaluated. For a parasitological diagnosis, Baermann and Hoffman et al.'s methods were applied. The immunological diagnosis involved the indirect fluorescence antibody test, ELISA and Western blotting to detect IgG antibodies. The frequency of positive S. stercoralis serology in diabetics was 23% versus 7.1% in the control group (P<0.05). The odds ratio for diabetics was 3.9 (CI, 1.6-15.9, P<0.05). Diabetic patients with HbA(1c)< or =7 had a greater chance of testing negatively for S. stercoralis infection (OR: 1.5, P>0.05). Provided there are related cases of disseminated strongyloidiasis in diabetics and there is a higher frequency of asymptomaticity of the infection in this group, the immunological screening of these patients at risk could prevent severe and fatal outcomes of the disease.