Genetically modified indigenous Pseudomonas aeruginosa drove bacterial community to change positively toward microbial enhanced oil recovery applications.

AIMS: Microbial enhanced oil recovery (MEOR) is cost-effective and eco-friendly for oil exploitation. Genetically modified biosurfactants-producing high-yield strains are promising for ex-situ MEOR. However, can they survive and produce biosurfactants in petroleum reservoirs for in-situ MEOR? What is their effect on the native bacterial community? METHODS AND RESULTS: A genetically modified indigenous biosurfactants-producing strain Pseudomonas aeruginosa PrhlAB was bioaugmented in simulated reservoir environments. Pseudomonas aeruginosa PrhlAB could stably colonize in simulated reservoirs. Biosurfactants (200 mg l-1) were produced in simulated reservoirs after bio-augmenting strain PrhlAB. The surface tension of fluid was reduced to 32.1 mN m-1. Crude oil was emulsified with an emulsification index of 60.1%. Bio-augmenting strain PrhlAB stimulated the MEOR-related microbial activities. Hydrocarbon-degrading bacteria and biosurfactants-producing bacteria were activated, while the hydrogen sulfide-producing bacteria were inhibited. Bio-augmenting P. aeruginosa PrhlAB reduced the diversity of bacterial community, and gradually simplified the species composition. Bacteria with oil displacement potential became dominant genera, such as Shewanella, Pseudomonas, and Arcobacter. CONCLUSIONS: Culture-based and sequence-based analyses reveal that genetically modified biosurfactants-producing strain P. aeruginosa PrhlAB are promising for in-situ MEOR as well.

High mono-rhamnolipids production by a novel isolate Pseudomonas aeruginosa LP20 from oily sludge: characterization, optimization, and potential application.

This study aims to isolate microbial strains for producing mono-rhamnolipids with high proportion. Oily sludge is rich in petroleum and contains diverse biosurfactant-producing strains. A biosurfactant-producing strain LP20 was isolated from oily sludge, identified as Pseudomonas aeruginosa based on phylogenetic analysis of 16S rRNA. High-performance liquid chromatography-mass spectrometry results indicated that biosurfactants produced from LP20 were rhamnolipids, mainly containing Rha-C8-C10, Rha-C10-C10, Rha-Rha-C8-C10, Rha-Rha-C10-C10, Rha-C10-C12:1, and Rha-C10-C12. Interestingly, more mono-rhamnolipids were produced by strain LP20 with a relative abundance of 64.5%. Pseudomonas aeruginosa LP20 optimally produced rhamnolipids at a pH of 7.0 and a salinity of 0.1% using glycerol and nitrate. The culture medium for rhamnolipids by strain LP20 was optimized by response surface methodology. LP20 produced rhamnolipids up to 6.9 g L-1, increased by 116%. Rhamnolipids produced from LP20 decreased the water surface tension to 28.1 mN m-1 with a critical micelle concentration of 60 mg L-1. The produced rhamnolipids emulsified many hydrocarbons with EI24 values higher than 56% and showed antimicrobial activity against Staphylococcus aureus and Cladosporium sp. with inhibition rates 48.5% and 17.9%, respectively. Pseudomonas aeruginosa LP20 produced more proportion of mono-rhamnolipids, and the LP20 rhamnolipids exhibited favorable activities and promising potential in microbial-enhanced oil recovery, bioremediation, and agricultural biocontrol.

Glycerol or crude glycerol as substrates make Pseudomonas aeruginosa achieve anaerobic production of rhamnolipids.

BACKGROUND: The anaerobic production of rhamnolipids is significant in research and application, such as foamless fermentation and in situ production of rhamnolipids in the anoxic environments. Although a few studies reported that some rare Pseudomonas aeruginosa strains can produce rhamnolipids anaerobically, the decisive factors for anaerobic production of rhamnolipids were unknown. RESULTS: Two possible hypotheses on the decisive factors for anaerobic production of rhamnolipids by P. aeruginosa were proposed, the strains specificity of rare P. aeruginosa (hypothesis 1) and the effect of specific substrates (hypothesis 2). This study assessed the anaerobic growth and rhamnolipids synthesis of three P. aeruginosa strains using different substrates. P. aeruginosa strains anaerobically grew well using all the tested substrates, but glycerol was the only carbon source that supported anaerobic production of rhamnolipids. Other carbon sources with different concentrations still failed for anaerobic production of rhamnolipids by P. aeruginosa. Nitrate was the excellent nitrogen source for anaerobic production of rhamnolipids. FTIR spectra analysis confirmed the anaerobically produced rhamnolipids by P. aeruginosa using glycerol. The anaerobically produced rhamnolipids decreased air-water surface tension to below 29.0 mN/m and emulsified crude oil with EI24 above 65%. Crude glycerol and 1, 2-propylene glycol also supported the anaerobic production of rhamnolipids by all P. aeruginosa strains. Prospects and bottlenecks to anaerobic production of rhamnolipids were also discussed. CONCLUSIONS: Glycerol substrate was the decisive factor for anaerobic production of rhamnolipids by P. aeruginosa. Strain specificity resulted in the different anaerobic yield of rhamnolipids. Crude glycerol was one low cost substrate for anaerobic biosynthesis of rhamnolipids by P. aeruginosa. Results help advance the research on anaerobic production of rhamnolipids, deepen the biosynthesis theory of rhamnolipids and optimize the anaerobic production of rhamnolipids.

Athermalization of dual-waveband infrared systems containing diffractive optical elements via optical-digital joint design.

Because of material limitations, achieving an athermal design for dual-waveband infrared systems is difficult. This study integrates single-layer diffractive elements to reduce the volume and weight of such a design and introduces optical-digital joint methods to eliminate the impact of low diffraction efficiency. To achieve athermalization, temperature polychromatic integral diffraction efficiency and temperature integral wavelength weight are incorporated in the point spread function (PSF) model. Influence of low diffraction efficiency is eliminated via subsequent algorithm processing. Accordingly, athermal design and processing of a cooled dual-waveband infrared system is achieved and verified via experimental results.

Achromatic annular folded lens with reflective-diffractive optics.

This paper proposes an achromatic annular folded lens (AFL) with a reflective-diffractive optical element (RDOE). We derive novel mathematical models of the diffraction efficiency and polychromatic integral diffraction efficiency (PIDE) of the RDOE and an expression for its microstructure height. An AFL with an RDOE made of an optical plastic substrate material is designed in the visible waveband. To minimize the influence of incident angle on the diffraction efficiency and PIDE, the microstructure height is optimized. The design results indicate that the lateral color of the AFL is corrected, the modulation transfer function considering the diffraction efficiency is larger than 0.25 at 111 cycles/mm for all field of views. The hybrid AFL outperforms the conventional refractive imaging system in terms of the system size, volume, and image quality under the same specifications. It can be used in new-generation miniaturized imaging systems.

Design of dual-band infrared zoom lens with multilayer diffractive optical elements.

A mid-wave infrared (MWIR)/long-wave infrared (LWIR) dual-band zoom lens design with multilayer diffractive optical elements (MLDOEs) is presented. The mathematical relationship between the substrate material selection for dual-band MLDOE and polychromatic integral diffraction efficiency (PIDE) is deduced in the oblique incident situation, and further, a method for optimal selection of substrate material is proposed to obtain the high PIDE in an incident angle range. In the optimization process, the optimal substrate material combination is selected based on the proposed method, and the principle of lens material replacement is discussed. After optimization, the 5× hybrid dual-band infrared zoom system is obtained, which consists of seven lenses. The modulation transfer function values in all configurations are larger than 0.5 and 0.3 in MWIR and LWIR, respectively. The distortion values are less than 2% both in MWIR and LWIR for all configurations.

Optical athermalization in the visible waveband using the 1+∑ method.

In this paper, the 1+∑ method, which is based on the double-lens equivalent model, is proposed to achieve optical athermalization in the visible waveband. The glass in the system was replaced by calculating and analyzing the synthetic weights of the thermal power and chromatic power. A complex aerial camera with long focal length was designed over a temperature difference of 100°C. The results demonstrated that the designed system could maintain good image quality and stable optical performance under large temperature differences.

PSF model for diffractive optical elements with improved imaging performance in dual-waveband infrared systems.

Single-layer diffractive optical elements (SLDOEs) have advantages in terms of configuration, fabrication, range of angles and cost; however, the diffraction efficiency decreases sharply with wavelength deviating from the design wavelength, especially for dual-waveband imaging, causing apparent image blur. We propose a point spread function (PSF) model affected by the diffraction efficiency, which is called PSFDOE, and a method of restoring the blurred image to improve imaging performance in dual-waveband infrared systems with an SLDOE. Then, a design example of cooled MWIR and LWIR is presented. Furthermore, imaging simulations with different grades noises and restorations are conducted. Results reveal that the PSFDOE model can significantly improve the image blur caused by the decreased diffraction efficiency.

Design of a built-in baffle for a Ritchey-Chretien optical system.

A baffle effectively prevents direct rays of stray light from entering an optical system. Existing design methods can result in very long outer baffle lengths, increasing the weight and volume of the optical system, or large secondary mirror baffle sizes, increasing the central obscuration and resulting in the reduction of the illumination beam on the image. To overcome these challenges, a design method for a built-in baffle is presented. The designed built-in baffle for a typical Ritchey-Chretien optical system is compared with a conventionally designed baffle. The results show that when the length of outer baffle is shortened from 3456 to 2349.9 mm, the modulation transfer function (MTF) increases from 0.361 to 0.421 in case 1, and when the length is shortened from 3456 to 1924.2 mm, the MTF increases from 0.361 to 0.408 in case 2. Meanwhile, the stray light suppression capability is close to that of the traditional method.

Effect of the surface roughness on the efficiency of diffractive optical elements.

The proposed study aims to investigate the effect of surface roughness on the efficiency of diffractive optical elements (DOEs) used in imaging optical systems. A mathematical model describing the influence of surface roughness on diffraction efficiency and polychromatic integral diffraction efficiency (PIDE) is presented, and its effects are analyzed and simulated. Examples of single-layer DOEs containing PMMA substrate and multi-layer DOEs composed of a combination of PMMA and polycarbonate substrates for each layer used in the visible region are analyzed. The results can be used to analyze the effects of surface roughness on the diffraction efficiency and PIDE of DOEs.

Optimization method of multilayer diffractive optical elements with consideration of ambient temperature.

A method for the optimal design of multilayer diffractive optical elements (MLDOEs) with consideration of ambient temperature is presented to improve the image quality over the entire temperature range. The relationship between diffraction efficiency and temperature is analyzed, and an optimization process of surface relief height for the MLDOEs is given. A practical 3-5 µm athermal hybrid optical system with a double-layer diffractive optical element is designed in the temperature range from -20°C to 60°C, and the image quality of two hybrid optical systems with optimized MLDOE and original MLDOE is compared. The result shows that the comprehensive modulation transfer function is obviously improved in the whole working temperature range. This method can be used during the passive athermalization hybrid optical system design with MLDOEs.

Optimal design method on diffractive optical elements with antireflection coatings.

The effect of antireflection coatings on diffraction efficiency of diffractive optical elements (DOEs) was studied and the mathematical model of diffraction efficiency affected by antireflection coatings for DOEs is presented. We found antireflection coatings can cause a significant reduction on diffraction efficiency at the designed, or the central wavelength. In order to solve this problem, we proposed a method to keep 100% diffraction efficiency at the designed wavelength by ensuring the 2π phase induced by DOEs and the antireflection coatings. Diffraction efficiency affected by antireflection coatings for DOEs with consideration of antireflection coatings are simulated. Analysis results can be utilized for refractive-diffractive hybrid imaging optical system optimal design and image quality evaluation.

Substrate material selection method for multilayer diffractive optics in a wide environmental temperature range.

We present a substrate material selection method for multilayer diffractive optical elements (MLDOEs) to obtain high polychromatic integral diffraction efficiency (PIDE) in a wide environmental temperature range. The extended expressions of the surface relief heights for the MLDOEs are deduced with consideration of the influence of the environmental temperature. The PIDE difference Δη¯(λ) and PIDE change factor F are introduced to select a reasonable substrate material combination. A smaller value of Δη¯(λ) or F indicates a smaller decrease of the PIDE in a wide temperature range, and the corresponding substrate material combination is better. According to the deduced relation, double-layer and three-layer DOEs with different combinations are discussed. The results show that IRG26 and zinc sulfide is the best substrate material combination in the infrared waveband for double-layer DOEs, and polycarbonate is more reasonable than polymethyl methacrylate as the middle filling optical material for three-layer DOEs when the two substrate materials are the same.

Aberration and boresight error correction for conformal aircraft windows using the inner window surface and tilted fixed correctors.

A static solution to aberrations and boresight error for tilted conformal aircraft windows at different look angles is reported. The solution uses the inner window surface to correct the window aberrations at a 0° look angle and uses fixed correctors behind the window to correct the residual window aberrations at other look angles. Then, the boresight error for the window at different look angles is corrected by tilting the fixed correctors. The principle of the solution is discussed, and a design example shows that the solution is effective in correcting the aberrations and boresight error for a tilted conformal aircraft window at different look angles.

High diffraction efficiency of three-layer diffractive optics designed for wide temperature range and large incident angle.

A mathematical model of diffraction efficiency and polychromatic integral diffraction efficiency affected by environment temperature change and incident angle for three-layer diffractive optics with different dispersion materials is put forward, and its effects are analyzed. Taking optical materials N-FK5 and N-SF1 as the substrates of multilayer diffractive optics, the effect on diffraction efficiency and polychromatic integral diffraction efficiency with intermediate materials POLYCARB is analyzed with environment temperature change as well as incident angle. Therefore, three-layer diffractive optics can be applied in more wide environmental temperature ranges and larger incident angles for refractive-diffractive hybrid optical systems, which can obtain better image quality. Analysis results can be used to guide the hybrid imaging optical system design for optical engineers.

Tolerance analysis of multilayer diffractive optics based on polychromatic integral diffraction efficiency.

Multilayer diffractive optical elements (MLDOEs) can achieve high diffraction efficiency for broadband wavelength. Polychromatic integral diffraction efficiency (PIDE) is the key concern for evaluating diffraction efficiency over the waveband. The modulation transfer function of a hybrid refractive-diffractive optical system is directly affected by the PIDE. The relationship between PIDE and continuous manufacturing errors for microstructure heights and periodic widths of MLDOEs is studied theoretically in this paper, and an example of MLDOEs is discussed in the visible waveband. The analysis results can be used for manufacturing error control in microstructure heights and periodic widths.

Microbial abundance and community composition influence production performance in a low-temperature petroleum reservoir.

Enhanced oil recovery using indigenous microorganisms has been successfully applied in the petroleum industry, but the role of microorganisms remains poorly understood. Here, we investigated the relationship between microbial population dynamics and oil production performance during a water flooding process coupled with nutrient injection in a low-temperature petroleum reservoir. Samples were collected monthly over a two-year period. The microbial composition of samples was determined using 16S rRNA gene pyrosequencing and real-time quantitative polymerase chain reaction analyses. Our results indicated that the microbial community structure in each production well microhabitat was dramatically altered during flooding with eutrophic water. As well as an increase in the density of microorganisms, biosurfactant producers, such as Pseudomonas, Alcaligenes, Rhodococcus, and Rhizobium, were detected in abundance. Furthermore, the density of these microorganisms was closely related to the incremental oil production. Oil emulsification and changes in the fluid-production profile were also observed. In addition, we found that microbial community structure was strongly correlated with environmental factors, such as water content and total nitrogen. These results suggest that injected nutrients increase the abundance of microorganisms, particularly biosurfactant producers. These bacteria and their metabolic products subsequently emulsify oil and alter fluid-production profiles to enhance oil recovery.

Improving Rhamnolipids Biosynthesis in Pseudomonas sp. L01 through Atmospheric and Room-Temperature Plasma (ARTP) Mutagenesis.

Biosurfactants have significant applications in various industries, including microbial-enhanced oil recovery (MEOR). While the state-of-the-art genetic approaches can generate high-yield strains for biosurfactant production in fermenters, there remains a critical challenge in enhancing biosurfactant-producing strains for use in natural environments with minimal ecological risks. The objectives of this work are enhancing the strain's capacity for rhamnolipids production and exploring the genetic mechanisms for its improvement. In this study, we employed atmospheric and room-temperature plasma (ARTP) mutagenesis to enhance the biosynthesis of rhamnolipids in Pseudomonas sp. L01, a biosurfactant-producing strain isolated from petroleum-contaminated soil. Following ARTP treatment, we identified 13 high-yield mutants, with the highest yield of 3.45 ± 0.09 g/L, representing a 2.7-fold increase compared to the parent strain. To determine the genetic mechanisms behind the enhanced rhamnolipids biosynthesis, we sequenced the genomes of the strain L01 and five high-yield mutants. A comparative genomic analysis suggested that mutations in genes related to the synthesis of lipopolysaccharides (LPS) and the transport of rhamnolipids may contribute to the improved biosynthesis. To the best of our knowledge, this is the first instance of utilizing the ARTP approach to improve rhamnolipid production in Pseudomonas strains. Our study provides valuable insights into the enhancement of biosurfactant-producing strains and the regulatory mechanisms of rhamnolipids biosynthesis.

Effects of manufacturing errors on diffraction efficiency for multilayer diffractive optical elements.

The effect of manufacturing errors on diffraction efficiency for multilayer diffractive optical elements (MLDOEs) used in imaging optical systems is discussed in this paper. The relationship of diffraction efficiency and depth-scaling errors are analyzed for two different cases: the two relative depth-scaling errors change in the same sign and in the opposite sign. For the first condition, the corresponding diffraction efficiency decreases more slowly. The effect of periodic width errors on diffraction efficiency is also evaluated. When the two major manufacturing errors coexist, the magnitude of the decrease of diffraction efficiency is analyzed for MLDOEs. The result can be used for analyzing the effects of the manufacturing errors on diffraction efficiency for MLDOEs.

Calculation and evaluation of narcissus for diffractive surfaces in infrared systems.

In infrared optical systems, the narcissus effect for diffractive surfaces should be calculated with specific diffraction orders based on the diffraction efficiency. It is shown in this work that the diffraction order of maximum diffraction efficiency varies with the change of the incident angle and wavelength of the backward-traced narcissus flux. Meanwhile, yni, which is the paraxial evaluation criterion of narcissus intensity for a refractive surface, is modified considering diffraction when a ray passes through diffractive surfaces, and a practical example has been given. The analysis can be used to calculate and control the narcissus intensity in infrared optical systems with diffractive surfaces.