Prognostic implications of residual disease tumor-infiltrating lymphocytes and residual cancer burden in triple-negative breast cancer patients after neoadjuvant chemotherapy.

BACKGROUND: For primary triple-negative breast cancer (TNBC) treated with neoadjuvant chemotherapy (NAC), higher pretreatment tumor-infiltrating lymphocytes (TILs) correlates with increased pathologic complete response (pCR) rates, and improved survival. We evaluated the added prognostic value of residual disease (RD) TILs to residual cancer burden (RCB) in predicting survival post-NAC. PATIENTS AND METHODS: We combined four TNBC NAC patient cohorts who did not achieve pCR. RD TILs were investigated for associations with recurrence-free survival (RFS), and overall survival (OS) using Cox models with stromal TILs as a continuous variable (per 10% increment). The likelihood ratio test was used to evaluate added prognostic value of RD TILs. RESULTS: A total of 375 RD TNBC samples were evaluable for TILs and RCB. The median age was 50 years, with 62% receiving anthracycline/taxane chemotherapy. The RCB class after NAC was 11%, 50%, and 39% for I, II, and III, respectively. The median RD TIL level was 20% (IQR 10-40). There was a positive correlation between RD TIL levels and CD8+ T-cell density (ρ = 0.41). TIL levels were significantly lower with increasing post-NAC tumor (P = 0.005), nodal stage (P = 0.032), but did not differ by RCB class (P = 0.84). Higher RD TILs were significantly associated with improved RFS (HR: 0.86; 95% CI 0.79-0.92; P < 0.001), and improved OS (HR: 0.87; 95% CI 0.80-0.94; P < 0.001), and remained significant predictors in multivariate analysis (RFS P = 0.032; OS P = 0.038 for OS). RD TILs added significant prognostic value to multivariate models including RCB class (P < 0.001 for RFS; P = 0.021 for OS). The positive prognostic effect of RD TILs significantly differed by RCB class for RFS (PInt=0.003) and OS (PInt=0.008) with a greater magnitude of positive effect observed for RCB class II than class III. CONCLUSIONS: TIL levels in TNBC RD are significantly associated with improved RFS and OS and add further prognostic information to RCB class, particularly in RCB class II.

Laying hen production responses to least cost rations formulated with stochastic programming or linear programming with a margin of safety.

An experiment with 480 DeKalb DK laying hens was conducted to study the effect of rations formulated with stochastic programming (STCH) or linear programming with a margin of safety (LPMS) over 12, 28-d periods. Rations were formulated to guarantee the requirement of methionine and lysine > or = 69%, in all rations, and Ca and P > or = either 69 or 90%. The four rations were: LPMS69 with Ca and P > or = 69%, LPMS90 with Ca and P > or = 90%, STCH69 with Ca and P > or = 69%, and STCH90 with Ca and P > or = 90%. Rations formulated with STCH were lower in cost than LPMS rations for respective probability levels. Costs per metric ton for LPMS69, LPMS90, STCH69, and STCH90 were $155.70, $157.71, $155.00, and $156.30, respectively. Compared to STCH rations, LPMS rations were overformulated in nutrients. There was no difference (P > .05) in performance for hen-housed egg production, hen-day egg production, feed per dozen eggs, mortality, egg weight, or eggshell percentage.

Effect of day to day variation of dietary energy on residual feed intake of laying hens.

An experiment was conducted that examined the effect of day-to-day variability of dietary energy on feed intake behavior. Three levels of average daily dietary ME composition (2,580, 2,814, and 3,009 kcal/kg) and three levels of day-to-day variability of ME (low, medium, and high) were assigned to nine groups of DeKalb-XL laying hens in a 3x3 factorial design. Each of the nine treatments contained 35 individually caged birds. Daily measurements of feed intake, egg production, and egg mass were taken for each bird in this 56-d experiment. Pre- and postexperiment body weights were taken at 23 and 31 wk of age. Increasing levels of day-to-day variance of ME were associated with increased feed consumption for each mean level of ME (P < 0.01) and increased variability of day-to-day feed intake for the 2,580 kcal/kg mean level (P < 0.01). No significant differences (P < 0.01) in egg production egg mass, or body weight were observed.