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The hemibiotrophic bacterial pathogen Pseudomonas syringae infects a range of plant species and causes enormous economic losses. Auxin and WRKY transcription factors play crucial roles in plant responses to P. syringae, but their functional relationship in plant immunity remains unclear. Here, we characterized tomato (Solanum lycopersicum) SlWRKY75, which promotes defenses against P. syringae pv. tomato (Pst) DC3000 by regulating plant auxin homeostasis. Overexpressing SlWRKY75 resulted in low free indole-3-acetic acid (IAA) levels, leading to attenuated auxin signaling, decreased expansin transcript levels, upregulated expression of PATHOGENESIS-RELATED GENES (PRs) and NONEXPRESSOR OF PATHOGENESIS-RELATED GENE 1 (NPR1), and enhanced tomato defenses against Pst DC3000. RNA interference-mediated repression of SlWRKY75 increased tomato susceptibility to Pst DC3000. Yeast one-hybrid, electrophoretic mobility shift assays, and luciferase activity assays suggested that SlWRKY75 directly activates the expression of GRETCHEN HAGEN 3.3 (SlGH3.3), which encodes an IAA-amido synthetase. SlGH3.3 enhanced tomato defense against Pst DC3000 by converting free IAA to the aspartic acid (Asp)-conjugated form IAA-Asp. In addition, SlWRKY75 interacted with a tomato valine-glutamine (VQ) motif-containing protein 16 (SlVQ16) in vivo and in vitro. SlVQ16 enhanced SlWRKY75-mediated transcriptional activation of SlGH3.3 and promoted tomato defense responses to Pst DC3000. Our findings illuminate a mechanism in which the SlVQ16-SlWRKY75 complex participates in tomato pathogen defense by positively regulating SlGH3.3-mediated auxin homeostasis.
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Regulação da Expressão Gênica de Plantas , Homeostase , Ácidos Indolacéticos , Doenças das Plantas , Proteínas de Plantas , Pseudomonas syringae , Solanum lycopersicum , Fatores de Transcrição , Solanum lycopersicum/microbiologia , Solanum lycopersicum/genética , Solanum lycopersicum/metabolismo , Solanum lycopersicum/imunologia , Ácidos Indolacéticos/metabolismo , Pseudomonas syringae/fisiologia , Pseudomonas syringae/patogenicidade , Fatores de Transcrição/metabolismo , Fatores de Transcrição/genética , Proteínas de Plantas/metabolismo , Proteínas de Plantas/genética , Doenças das Plantas/microbiologia , Doenças das Plantas/imunologia , Doenças das Plantas/genética , Imunidade Vegetal/genética , Plantas Geneticamente ModificadasRESUMO
Chilling stress seriously impairs photosynthesis and activates a series of molecular responses in plants. Previous studies have shown that ETHYLENE INSENSITIVE 3 (EIN3) and EIN3-like (SlEIL) proteins mediate ethylene signaling and reduce plant tolerance to freezing in tomato (Solanum lycopersicum). However, the specific molecular mechanisms underlying an EIN3/EILs-mediated photoprotection pathway under chilling stress are unclear. Here, we discovered that salicylic acid (SA) participates in photosystem II (PSII) protection via SlEIL2 and SlEIL7. Under chilling stress, the phenylalanine ammonia-lyase gene SlPAL5 plays an important role in the production of SA, which also induces WHIRLY1 (SlWHY1) transcription. The resulting accumulation of SlWHY1 activates SlEIL7 expression under chilling stress. SlEIL7 then binds to and blocks the repression domain of the heat shock factor SlHSFB-2B, releasing its inhibition of HEAT SHOCK PROTEIN 21 (HSP21) expression to maintain PSII stability. In addition, SlWHY1 indirectly represses SlEIL2 expression, allowing the expression of l-GALACTOSE-1-PHOSPHATE PHOSPHATASE3 (SlGPP3). The ensuing higher SlGPP3 abundance promotes the accumulation of ascorbic acid (AsA), which scavenges reactive oxygen species produced upon chilling stress and thus protects PSII. Our study demonstrates that SlEIL2 and SlEIL7 protect PSII under chilling stress via two different SA response mechanisms: one involving the antioxidant AsA and the other involving the photoprotective chaperone protein HSP21.
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Solanum lycopersicum , Solanum lycopersicum/genética , Complexo de Proteína do Fotossistema II/metabolismo , Ácido Salicílico , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Ácido Ascórbico/metabolismo , Etilenos , Temperatura BaixaRESUMO
ETHYLENE-INSENSITIVE 3/ETHYLENE-INSENSITIVE 3-LIKEs (EIN3/EILs) are important ethylene response factors during fruit ripening. Here, we discovered that EIL2 controls carotenoid metabolism and ascorbic acid (AsA) biosynthesis in tomato (Solanum lycopersicum). In contrast to the red fruits presented in the wild type (WT) 45 d after pollination, the fruits of CRISPR/Cas9 eil2 mutants and SlEIL2 RNA interference lines (ERIs) showed yellow or orange fruits. Correlation analysis of transcriptome and metabolome data for the ERI and WT ripe fruits revealed that SlEIL2 is involved in ß-carotene and AsA accumulation. ETHYLENE RESPONSE FACTORs (ERFs) are the typical components downstream of EIN3 in the ethylene response pathway. Through a comprehensive screening of ERF family members, we determined that SlEIL2 directly regulates the expression of 4 SlERFs. Two of these, SlERF.H30 and SlERF.G6, encode proteins that participate in the regulation of LYCOPENE-ß-CYCLASE 2 (SlLCYB2), encoding an enzyme that mediates the conversion of lycopene to carotene in fruits. In addition, SlEIL2 transcriptionally repressed L-GALACTOSE 1-PHOSPHATE PHOSPHATASE 3 (SlGPP3) and MYO-INOSITOL OXYGENASE 1 (SlMIOX1) expression, which resulted in a 1.62-fold increase of AsA via both the L-galactose and myoinositol pathways. Overall, we demonstrated that SlEIL2 functions in controlling ß-carotene and AsA levels, providing a potential strategy for genetic engineering to improve the nutritional value and quality of tomato fruit.
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Solanum lycopersicum , beta Caroteno , beta Caroteno/metabolismo , Licopeno/metabolismo , Solanum lycopersicum/genética , Ácido Ascórbico/metabolismo , Galactose/metabolismo , Etilenos/metabolismo , Frutas/metabolismo , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismoRESUMO
Long running-in period and corrosion problems have greatly hindered the practical applications of poly(ethylene glycol) (PEG) lubricants. In this work, benzotriazole group-terminated carbon dots (BT-CDs) were specifically synthesized through a facile solvothermal method. The benzotriazole groups on BT-CD surfaces not only imparted them excellent dispersibility in the PEG base oil but also brought in outstanding anticorrosion ability for BT-CDs. With the aid of the coordination effects between benzotriazole groups and metal atoms, the BT-CDs could quickly and solidly adsorb onto the steel surface to form a dense adsorption layer, which resulted in an amazing phenomenon, i.e., the disappearance of the running-in period for the friction test. Adding 5.0 wt % BT-CDs reduced the friction and wear of PEG200 by 49.16 and 49.52%, respectively. When the duration was prolonged from 20 to 120 min, these values were further enlarged to 53.77 and 60.71%. The worn surface characterization demonstrated that the BT-CDs induced the generation of robust lubricating films on the frictional interfaces, accounting for their distinguished tribological performance. Considering the superior anticorrosion ability and the potential possibility of avoiding the running-in period, the BT-CDs are expected to be developed as particularly promising additives toward PEG.
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BACKGROUND: Domestication and introduction of dairy animals facilitated the permanent human occupation of the Tibetan Plateau. Yet the history of dairy pastoralism in the Tibetan Plateau remains poorly understood. Little is known how Tibetans adapted to milk and dairy products. RESULTS: We integrated archeological evidence and genetic analysis to show the picture that the dairy ruminants, together with dogs, were introduced from West Eurasia into the Tibetan Plateau since ~ 3600 years ago. The genetic admixture between the exotic and indigenous dogs enriched the candidate lactase persistence (LP) allele 10974A > G of West Eurasian origin in Tibetan dogs. In vitro experiments demonstrate that - 13838G > A functions as a LP allele in Tibetans. Unlike multiple LP alleles presenting selective signatures in West Eurasians and South Asians, the de novo origin of Tibetan-specific LP allele - 13838G > A with low frequency (~ 6-7%) and absence of selection corresponds - 13910C > T in pastoralists across eastern Eurasia steppe. CONCLUSIONS: Results depict a novel scenario of genetic and cultural adaptations to diet and expand current understanding of the establishment of dairy pastoralism in the Tibetan Plateau.
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Criação de Animais Domésticos , Povo Asiático , Dieta , Leite , Animais , Cães/genética , Humanos , Tibet , RuminantesRESUMO
The diversity of Central Asians has been shaped by multiple migrations and cultural diffusion. Although ancient DNA studies have revealed the demographic changes of the Central Asian since the Bronze Age, the contribution of the ancient populations to the modern Central Asian remains opaque. Herein, we performed high-coverage sequencing of 131 whole genomes of Indo-European-speaking Tajik and Turkic-speaking Kyrgyz populations to explore their genomic diversity and admixture history. By integrating the ancient DNA data, we revealed more details of the origins and admixture history of Central Asians. We found that the major ancestry of present-day Tajik populations can be traced back to the admixture of the Bronze Age Bactria-Margiana Archaeological Complex and Andronovo-related populations. Highland Tajik populations further received additional gene flow from the Tarim mummies, an isolated ancient North Eurasian-related population. The West Eurasian ancestry of Kyrgyz is mainly derived from Historical Era populations in Xinjiang of China. Furthermore, the recent admixture signals detected in both Tajik and Kyrgyz are ascribed to the expansions of Eastern Steppe nomadic pastoralists during the Historical Era.
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DNA Antigo , Múmias , Povo Asiático/genética , Etnicidade , Fluxo Gênico , Genética Populacional , HumanosRESUMO
Activation of gasdermin D (GSDMD) and its concomitant cardiomyocyte pyroptosis are critically involved in multiple cardiac pathological conditions. Pharmacological inhibition or gene knockout of GSDMD could protect cardiomyocyte from pyroptosis and dysfunction. Thus, seeking and developing highly potent GSDMD inhibitors probably provide an attractive strategy for treating diseases targeting GSDMD. Through structure-based virtual screening, pharmacological screening and subsequent pharmacological validations, we preliminarily identified GSDMD inhibitor Y1 (GI-Y1) as a selective GSDMD inhibitor with cardioprotective effects. Mechanistically, GI-Y1 binds to GSDMD and inhibits lipid- binding and pyroptotic pore formation of GSDMD-N by targeting the Arg7 residue. Importantly, we confirmed the cardioprotective effect of GI-Y1 on myocardial I/R injury and cardiac remodeling by targeting GSDMD. More extensively, GI-Y1 also inhibited the mitochondrial binding of GSDMD-N and its concomitant mitochondrial dysfunction. The findings of this study identified a new drug (GI-Y1) for the treatment of cardiac disorders by targeting GSDMD, and provide a new tool compound for pyroptosis research.
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Cardiopatias , Traumatismo por Reperfusão , Humanos , Piroptose , Miócitos Cardíacos , Isquemia , Proteínas de Ligação a Fosfato , Proteínas Citotóxicas Formadoras de PorosRESUMO
The rise and expansion of Tibetan Empire in the 7th to 9th centuries AD affected the course of history across East Eurasia, but the genetic impact of Tibetans on surrounding populations remains undefined. We sequenced 60 genomes for four populations from Pakistan and Tajikistan to explore their demographic history. We showed that the genomes of Balti people from Baltistan comprised 22.6-26% Tibetan ancestry. We inferred a single admixture event and dated it to about 39-21 generations ago, a period that postdated the conquest of Baltistan by the ancient Tibetan Empire. The analyses of mitochondrial DNA, Y, and X chromosome data indicated that both ancient Tibetan males and females were involved in the male-biased dispersal. Given the fact that the Balti people adopted Tibetan language and culture in history, our study suggested the impact of Tibetan Empire on Baltistan involved dominant cultural and minor demic diffusion.
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Fluxo Gênico , Genoma Humano , Feminino , Humanos , Masculino , Paquistão , Tibet/etnologia , Sequenciamento Completo do GenomaRESUMO
BACKGROUND: Lymphocytic leukemia (LL) is a primary malignant tumor of hematopoietic tissue, which seriously affects the health of children and the elderly. The study aims to establish a new detection method for screening acute/chronic LL using time-resolved fluorescence immunoassay (TRFIA) via quantitative detection of S100 calcium binding protein A8 (S100A8) and leucine-rich alpha-2-glycoprotein 1 (LRG1) in serum. METHODS: Here a sandwich TRFIA was optimized and established: Anti-S100A8/LRG1 caputre antibodies immobilized on 96-well plates captured S100A8/LRG1, and then banded together with the anti-S100A8/LRG1 detection antibodies labeled with Europium(III) (Eu3+)/samarium(III) (Sm3+) chelates. Finally time resolved fluorometry measured the fluorescence intensity. RESULTS: The sensitivity of S100A8 was 1.15 ng/mL(LogY = 3.4027 + 0.4091 × LogX, R2 = 0.9828, P < 0.001, dynamic range: 2.1-10,000 ng/mL), and 3.2 ng/mL for LRG1 (LogY = 3.3009 + 0.4082 × LogX, R2 = 0.9748, P < 0.001, dynamic range: 4.0-10,000 ng/mL). The intra-assay and inter-assay CVs were low, ranging from 5.75% to 8.23% for S100A8 and 5.30% to 9.45% for LRG1 with high specificity and affinity in serum samples. Bland-Altman plots indicated TRFIA and ELISA kits have good agreement in clinical serum samples. Additionally, the cutoff values for S100A8 and LRG1 were 1849.18 ng/mL and 588.08 ng/mL, respectively. CONCLUSION: The present TRFIA method could be used for the quantitative detection of S100A8 and LRG1 in serum, and it has high sensitivity, accuracy and specificity. Clinically, this TRFIA method could be suitable for screening of LL via the quantitative detection of S100A8 and LRG1.
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Európio , Leucemia Linfocítica Crônica de Células B , Idoso , Proteínas de Ligação ao Cálcio , Criança , Fluorimunoensaio/métodos , Glicoproteínas , Humanos , Leucina , Leucemia Linfocítica Crônica de Células B/diagnóstico , Samário , Sensibilidade e EspecificidadeRESUMO
Phase-selective organogelators (PSOGs) have recently attracted more attention because of their advantages in handling oil spills and leaked organic solvents. However, it is difficult to separate and recover the organic phase and PSOGs from organic gels due to the strong interaction between them. Aiming to enhance the separation and recovery performance of the organic phase and PSOGs, we synthesized a series of pH-responsive PSOGs by using itaconic anhydride and fatty amines with carbon chain lengths of C12-C18. Here, PSOGs have an excellent gelation ability in that amounts of organic solvents and fuel oil can be solidified at a low concentration (<3 wt %). It is worth noting that these gels are stronger, which is more convenient for removal by a salvage operation. More importantly, compared with traditional organogelators, pH-responsive PSOGs can easily recover the organic phase and fuel oil with an adjustment of the pH without extraction or distillation. Because of the transformation between the hydrophilicity and hydrophobicity of PSOGs by pH stimulation, 83.15% PSOGs are recovered in three-cycle experiments. In addition, the recycled PSOGs can be used to realize the removal of the organic phase again. Herein, we find that pH-responsive PSOGs could be used as promising and sustainable materials for separating and recovering organic solvents/oils and PSOGs.
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Óleos Combustíveis , Poluição por Petróleo , Ácidos Carboxílicos , Géis/química , Concentração de Íons de Hidrogênio , Poluição por Petróleo/análise , Solventes/químicaRESUMO
OBJECTIVE: Trimetazidine (TMZ) exerts a strong inhibitory effect on ischemia/reperfusion (I/R) injury. Inflammation plays a key role in I/R injury. We hypothesized that TMZ may protect cardiomyocytes from I/R injury by inhibiting inflammation. METHODS: The left anterior descending coronary artery was ligated for 30 min followed by 6 h of reperfusion to establish a model of I/R injury. H9c2 cardiomyocytes were subjected to 2 h of hypoxia and 3 h of normoxic conditions to establish a model of hypoxia/reoxygenation (H/R) injury. We monitored the change in pyroptosis by performing Western blot analysis, microscopy and ELISA. RESULTS: I/R and H/R treatment stimulated gasdermin D-N domain (GSDMD-N) expression in cardiomyocytes (sham onefold vs. I/R 2.5-fold; control onefold vs. H/R 2.0-fold). Moreover, TMZ increased the viability of H9c2 cardiomyocytes subjected to H/R treatment (H/R 65.0% vs. H/R + TMZ 85.3%) and reduced the infarct size in vivo (I/R 47.0% vs. I/R + TMZ 28.3%). H/R and I/R treatment increased the levels of TLR4, MyD88, phospho-NF-κB p65 and the NLRP3 inflammasome; however, TMZ reduced the expression of these proteins. Additionally, TMZ inhibited noncanonical inflammasome signaling induced by I/R injury. CONCLUSIONS: In summary, TMZ alleviated pyroptosis induced by myocardial I/R injury through the TLR4/MyD88/NF-κB/NLRP3 inflammasome pathway. Therefore, TMZ represents an alternative treatment for myocardial I/R injury.
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Traumatismo por Reperfusão Miocárdica/tratamento farmacológico , Proteínas de Ligação a Fosfato/antagonistas & inibidores , Proteínas Citotóxicas Formadoras de Poros/antagonistas & inibidores , Piroptose/efeitos dos fármacos , Trimetazidina/farmacologia , Animais , Masculino , Traumatismo por Reperfusão Miocárdica/patologia , Miócitos Cardíacos/efeitos dos fármacos , Miócitos Cardíacos/patologia , NF-kappa B/fisiologia , Proteína 3 que Contém Domínio de Pirina da Família NLR/fisiologia , Proteínas de Ligação a Fosfato/fisiologia , Proteínas Citotóxicas Formadoras de Poros/fisiologia , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos , Receptor 4 Toll-Like/fisiologiaRESUMO
BACKGROUND: Depression has received a lot of attention as a common and serious illness. However, people are rarely aware of their current depression risk probabilities. We aimed to develop and validate a predictive model applicable to the risk of depression in US adults. METHODS: This study was conducted using the database of the National Health and Nutrition Examination Survey (NHANES, 2017-2012). In particular, NHANES (2007-2010) was used as the training cohort (n = 6015) for prediction model construction and NHANES (2011-2012) was used as the validation cohort (n = 2812) to test the model. Depression was assessed (defined as a binary variable) by the Patient Health Questionnaire (PHQ-9). Socio-demographic characteristics, sleep time, illicit drug use and anxious days were assessed using a self-report questionnaire. Logistic regression analysis was used to evaluate independent risk factors for depression. The nomogram has the advantage of being able to visualize complex statistical prediction models as risk estimates of individualized disease probabilities. Then, we developed two depression risk nomograms based on the results of logistic regression. Finally, several validation methods were used to evaluate the prediction performance of nomograms. RESULTS: The predictors of model 1 included gender, age, income, education, marital status, sleep time and illicit drug use, and model 2, furthermore, included anxious days. Both model 1 and model 2 showed good discrimination ability, with a bootstrap-corrected C index of 0.71 (95% CI, 0.69-0.73) and 0.85 (95% CI, 0.83-0.86), and an externally validated C index of 0.71 (95% CI, 0.68-0.74) and 0.83 (95% CI, 0.81-0.86), respectively, and had well-fitted calibration curves. The area under the receiver operating characteristic curve (AUC) values of the models with 1000 different weighted random sampling and depression scores of 10-17 threshold range were higher than 0.7 and 0.8, respectively. Calculated net reclassification improvement (NRI) and integrated discrimination improvement (IDI) showed the discrimination or accuracy of the prediction models. Decision curve analysis (DCA) demonstrated that the depression models were practically useful. The network calculators work for participants to make personalized predictions. CONCLUSIONS: This study presents two prediction models of depression, which can effectively and accurately predict the probability of depression as well as helping the U.S. civilian non-institutionalized population to make optimal treatment decisions.
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Drogas Ilícitas , Nomogramas , Adulto , Depressão/epidemiologia , Humanos , Inquéritos Nutricionais , Estudos Retrospectivos , Programa de SEERRESUMO
Reversible addition-fragmentation transfer (RAFT) dispersion polymerization of styrene was performed in an ethanol-water mixture using a Z-group carboxylated poly(N-acryloylmorpholine) (PNAM) macro-RAFT agent, and dialysis was performed against water to produce the PNAMx-PSy-COOH (PS = polystyrene) diblock copolymer latexes. This new formula is developed for the fabrication of pH-switchable copolymer latexes through an end-group response approach. The PNAM44-PS134-COOH latex is unstable at suitably low pH values (pH ≤ 4), and these aggregated spherical nanoparticles are redispersed successfully by adding base as determined by analysis of their dynamic light scattering (DLS) diameters and transmission electron microscopy (TEM) data. Negative zeta potential (-19.4 mV at 0.02% w/w) of the original latex indicated that carboxylic acid end-groups were anchored on the surface of the PS core via the polymerization-induced self-assembly (PISA) process and exposed to the solvent. Protonation of carboxylate groups reduces the degree of hydration of the PS core with a great impact on the free energy of the core/solvent interface, inducing the aggregation of PNAM44-PS134-COOH latex particles. A comparative experiment where the carboxylic acid end-group is designed on the PNAM stabilizer block proves that no pH-switchable behavior occurs in this case. Moreover, the vesicle-like nanoparticles composed of PNAM44-PS428-COOH copolymers have an apparently anionic character (zeta potential ≈ -33.5 mV at 0.02% w/w) and are still pH-switchable with a lower critical flocculation point (pH 2-3). More importantly, the latex composed of PNAM118-PS151-COOH diblock copolymers is insensitive to the solution pH.
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Two kinds of water-soluble tertiary amines (TAs), triethylamine (TEA, monoamine), and tetramethyltrimethylenediamine (TMA, diamine) were introduced into a NaOA stable oil-water (O/W) emulsion, respectively, and their dual reactivity to carbon dioxide was studied. TA was converted into bicarbonate after bubbling of CO2, which induced the increase of ionic strength of the aqueous phase, and formed ion pair with NaOA through electrostatic interaction. NaOA itself can also be protonated into oleic acid, which can be reverently deprotonated by alternating bubbles of CO2 at 25 °C and N2 at 50 °C, thus affecting the stability and demulsification process of the emulsion. In order to demonstrate TA's and NaOA's synergistic effect on CO2 responsiveness, gas chromatography-mass spectrometry, ζ potential, electrical conductivity, pH value, 1H nuclear magnetic resonance, morphological evolution, and interfacial tension were used to study the contributions of the single component and two components of NaOA, TEA, and TMA to emulsion stability and CO2 responsiveness, respectively. Combined with the composition distribution under different pH conditions, it was further proved that TAs had an effect on the stability and CO2 responsiveness of the NaOA emulsion.
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Myocardial injury and cardiovascular dysfunction are serious consequences of sepsis and contribute to high mortality. Currently, the pathogenesis of myocardial injury in sepsis is still unclear, and therapeutic approaches are limited. In this study, we investigated the protective effect of emodin on septic myocardial injury and the underlying mechanism. Lipopolysaccharide (LPS)-induced C57BL/6 mice and cardiomyocytes were used as models of sepsis in vivo and in vitro, respectively. The results showed that emodin alleviated cardiac dysfunction, myocardial injury and improved survival rate in LPS-induced septic mice. Emodin attenuated the levels of inflammatory cytokines and cardiac inflammation induced by LPS. Emodin reduced NOD-like receptor protein 3 (NLRP3) and Gasdermin D (GSDMD) expression in the heart tissue of LPS-induced septic mice. In vitro, emodin alleviated LPS-induced cell injury and inflammation in cardiomyocytes by inhibiting NLRP3 inflammasome activation. In addition, an NLRP3 inhibitor was used to further confirm the function of the NLRP3 inflammasome in LPS-induced myocardial injury. Taken together, our findings suggest that emodin improves LPS-induced myocardial injury and cardiac dysfunction by alleviating the inflammatory response and cardiomyocyte pyroptosis by inhibiting NLRP3 inflammasome activation, which provides a feasible strategy for preventing and treating myocardial injury in sepsis.
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Emodina , Inflamassomos , Proteína 3 que Contém Domínio de Pirina da Família NLR/antagonistas & inibidores , Sepse/tratamento farmacológico , Animais , Emodina/farmacologia , Coração/efeitos dos fármacos , Inflamassomos/antagonistas & inibidores , Lipopolissacarídeos/toxicidade , Camundongos , Camundongos Endogâmicos C57BL , Miocárdio/patologia , Miócitos Cardíacos/efeitos dos fármacos , Miócitos Cardíacos/patologia , Proteínas NLRRESUMO
CO2-responsive microemulsions based on ionic liquid 1,1,3,3-tetramethylguanidine-oleic acid (TMG-OA) have been designed to provide an approach for reducing the volatilization of amine in amine-containing microemulsions effectively and exhibit reversible transitions of microstructures. The ionic liquid TMG-OA was prepared by the direct neutralization of oleic acid (HOA) and 1,1,3,3-tetramethylguanidine (TMG, one of volatile and toxic amines). From the investigations of nuclear magnetic resonance hydrogen spectrum, pH, thermogravimetry, and automatic interface tension meter, the excellent properties of switchability, stability, and surface activity of TMG-OA were demonstrated, and then the ionic liquid-based microemulsions with CO2 response were prepared with TMG-OA (surfactant), HOA (oil phase), isopropyl alcohol (IPA, cosurfactant), and water. Interestingly, for microemulsions with a higher IPA content (47.42, 44.48 wt %), sizes of microemulsions are increased upon introducing CO2 and decreased upon addition of N2/65 °C. In addition, for microemulsions with a lower IPA content (26.22 wt %), the new microemulsions with different sizes are regenerated after the phase separation of emulsions generated by introducing CO2, and incomplete recovery of microemulsions can be observed upon addition of N2/65 °C. The reversible microstructures are induced by the swelling behavior and the reduced single phase area, which are caused by the reversible conversion between TMG-OA and HOA components.
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The derivatives of ferrocene with redox properties are widely used. Some studies have used complex synthesis processes to obtain surfactants with redox properties. In order to simplify the synthesis process, FA-DMDA-Ox, a surfactant with redox and pH dual responses, was prepared by simple electrostatic interaction between ferrocenecarboxylic acid (FA) and N,N-dimethyldodecylamine (DMDA). A stable oil-in-water emulsion was prepared by using FA-DMDA-Ox at 25 °C. When sodium sulfite was added to the emulsion, the emulsion was demulsified. This was due to the oxidized ferrocene group that was reduced from the charged hydrophilic state to the uncharged hydrophobic state, which destroyed the original surface activity. In addition, when added HCl or NaOH to the emulsion changed pH, demulsification was caused by the dissociation of FA-DMDA-Ox.
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N-Doped carbon dots (CDs) were directly synthesized with a high yield of 21.85% by one-pot pyrolysis of a gel composed of ethanolamine and 1-carboxyethyl-3-methylimidazolium chloride at 220 °C for 2 h. The as-synthesized CDs with a mean particle size of 7.8 nm were uniform, amorphous, and abundant in nitrogen content (23.15 wt%) and surface groups such as amide and hydroxyl. Thus, the CDs exhibited good water-solubility, bright blue excitation- and pH-dependent fluorescence with a quantum yield of 17.93%, and high ionic strength tolerance. In addition, a CD-based fluorescent sensor towards Cr(vi) ions with favorable sensitivity and selectivity was constructed based on the inner filter effect. Two good linear relationships between the concentration of Cr(vi) ions and the PL quenching efficiency were obtained in the ranges from 0.2 to 2 (R2 = 0.9965) and 2-40 µM (R2 = 0.9918), and the limits of detection (LOD = 3σ/S) were calculated as 0.018 and 0.25 µM, respectively. Importantly, this sensor was solid and capable of rapidly detecting Cr(vi) ions in tap water with detection ranges of 0.2-2 (R2 = 0.9817) and 2-60 µM (R2 = 0.9902), LODs of 0.048 and 0.40 µM, and recoveries of 102.1-106%.
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A series of CO2-responsive oil-in-water (O/W) emulsions were prepared by introducing hydrophobic tertiary amines (TAs) with varying alkane carbon numbers (ACNs) into the emulsion stabilized by sodium dodecyl benzene sulfonate (SDBS). TAs are converted to bicarbonate salts upon bubbling of CO2, which can form ion pairs with SDBS via electrostatic interaction, and then disrupt the stability of the emulsion. The reversible switch can be triggered by the removal of CO2. The ACN of TA, the concentration of SDBS/TA, the bubbling time of CO2, and the number of cycles are taken into account in order to study the controllable mechanism of these CO2-responsive emulsions. Because of the improved miscibility with oil, the ion pairs with TAs of larger ACNs can much more easily adhere to the oil phase, and then speed up the rupture rate of the oil droplets. The corresponding demulsification process is tracked by studying the interfacial tension, the zeta potential of the droplets, and microscope snapshots of all the systems. The UV-vis spectrophotometer analysis of the water phase and the 1H-nuclear magnetic resonance (1H NMR) test are further designed to comprehend the significance of ACNs and the solubility product of the formed ion pairs.
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In this work, we present a CMOS-integrated low-noise junction field-effect transistor (JFET) developed in a standard 0.18 pm CMOS process. These JFETs reduce input-referred flicker noise power by more than a factor of 10 when compared to equally sized n-channel MOS devices by eliminating oxide interfaces in contact with the channel. We show that this improvement in device performance translates into a factor-of-10 reduction in the input-referred noise of integrated CMOS operational amplifiers when JFET devices are used at the input, significant for many applications in bioelectronics.