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1.
J Gen Virol ; 95(Pt 4): 898-904, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24421111

RESUMO

Orthoreoviruses have been associated with a variety of diseases in domesticated poultry and wild-living birds. In 2002, a reovirus strain named Tvärminne avian virus (TVAV), was identified in Finland in a crow showing neurological disorders. The objective of this study was the molecular characterization of this novel reovirus strain. Genome sequencing was performed by combining semiconductor sequencing and traditional capillary sequencing. Sequence and phylogenetic analyses showed that TVAV shares low nucleotide sequence identity with other reoviruses (range for each gene, 31-72 %) including strains belonging to the species Avian orthoreovirus. The most closely related reovirus strain was an isolate identified in Steller sea lion. Our data indicate that TVAV is a divergent reovirus of avian origin that may be the first representative of a distinct virus species within the genus Orthoreovirus.


Assuntos
Genoma Viral , Orthoreovirus Aviário/classificação , Orthoreovirus Aviário/genética , RNA Viral/genética , Análise de Sequência de DNA , Animais , Aves , Análise por Conglomerados , Finlândia , Dados de Sequência Molecular , Orthoreovirus Aviário/isolamento & purificação , Filogenia
2.
Arch Virol ; 159(8): 2165-9, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24573219

RESUMO

The complete genomic sequence of a Hungarian goose orthoreovirus strain (D20/99) is reported in this study. The genome of D20/99 is 22,969 bp in length (range, 3958 bp for L1 to 1124 bp for S4) and encodes 11 putative proteins. Pairwise sequence comparisons and phylogenetic analyses indicated that D20/99 shares genetic signatures with some contemporary Chinese duck and goose reovirus strains, except for the µA, µNS and σA protein coding genes, which represented independent genetic lineages. This study implies a greater genetic diversity among waterfowl-origin orthoreoviruses than hitherto recognized.


Assuntos
Doenças das Aves/virologia , Genoma Viral , Orthoreovirus Aviário/genética , Infecções por Reoviridae/veterinária , Sequência de Aminoácidos , Animais , Animais Selvagens/virologia , Sequência de Bases , Gansos , Dados de Sequência Molecular , Orthoreovirus Aviário/classificação , Orthoreovirus Aviário/isolamento & purificação , Filogenia , Infecções por Reoviridae/virologia
3.
Arch Virol ; 158(12): 2583-8, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23771766

RESUMO

Avian orthoreoviruses have been associated with a variety of diseases in chickens, including tenosynovitis, runting-stunting syndrome, hepatitis, myocarditis, osteoporosis, respiratory diseases, and central nervous system disease. The primary objective of our study was the molecular characterization of an avian reovirus strain, T1781, which was isolated from a broiler chicken with a central nervous system disorder in Hungary during 2012. The complete genome sequence was determined using a traditional sequencing method after cell culture adaptation of the strain. Sequence and phylogenetic analyses showed that T1781 shared only moderate nucleic acid sequence identity in several genes to previously analyzed reovirus strains from chickens, and each gene formed separate branches in the corresponding phylogenetic trees. The maximum nucleotide sequence identities of strain T1781 genes to reference avian reovirus strains ranged from 79 % to 90 %. Collectively, our analyses indicated that T1781 is a divergent chicken reovirus strain. The genetic background of this and other avian reoviruses associated with various disease manifestations needs further investigation.


Assuntos
Infecções do Sistema Nervoso Central/veterinária , Genoma Viral , Orthoreovirus Aviário/genética , Doenças das Aves Domésticas/virologia , RNA Viral/genética , Infecções por Reoviridae/veterinária , Análise de Sequência de DNA , Animais , Infecções do Sistema Nervoso Central/virologia , Galinhas , Análise por Conglomerados , Hungria , Orthoreovirus Aviário/isolamento & purificação , Filogenia , Infecções por Reoviridae/virologia , Homologia de Sequência do Ácido Nucleico
4.
Virus Genes ; 42(1): 82-9, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21116842

RESUMO

Avian orthoreoviruses (ARVs) are responsible for considerable economic losses in broiler chickens; yet, the genetic characterization of most ARV strains is limited to a few genes, and the full coding region has been determined for only S1133 and 138, two ARV strains associated with tenosynovitis. Recently, in parts of the United States, ARVs with novel neutralization antigen type were isolated from chickens afflicted with runting-stunting syndrome. One such strain, AVS-B, was selected for full genome sequencing and phylogenetic analysis. The complete genome was 23,494 bp in size and included 12 open reading frames. The lengths of the coding regions, as well as those of the 5' and 3' ends, were fairly well conserved between AVS-B and other reference strains. In pairwise comparisons to the S1133 and 138 strains, the AVS-B strain shared a wide range of sequence identities along each genome segment, i.e., a range of 54-55% for the σC coding region of S1 genome segment and 91-93% for the S2 genome segment. Phylogenetic analyses of individual genes of AVS-B did not identify any single common ancestor among more completely characterized ARV strains for which sequence data are available. One exception to this lack of identity was strain 138, which shared 90-93% nt identity with AVS-B along seven of ten genome segments; only M2, M3, and S1 segments of these strains shared lower sequence identities. Collectively, our analyses indicated that multiple reassortment events and strong divergence caused by the accumulation of point mutations could have led to the observed assortment and genetic heterogeneity of the AVS-B genome.


Assuntos
Galinhas/virologia , Genoma Viral , Orthoreovirus Aviário/genética , Filogenia , Animais , Proteínas do Capsídeo/genética , Galinhas/crescimento & desenvolvimento , Heterogeneidade Genética , Fases de Leitura Aberta , Orthoreovirus Aviário/patogenicidade , Doenças das Aves Domésticas/virologia , RNA Viral/genética , Proteínas de Ligação a RNA/genética , RNA Polimerase Dependente de RNA/genética , Infecções por Reoviridae/veterinária , Infecções por Reoviridae/virologia , Proteínas Virais/genética , Proteínas Virais Reguladoras e Acessórias/genética
5.
Virus Res ; 215: 99-103, 2016 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-26597720

RESUMO

Avian orthoreoviruses cause various diseases in wild birds and domesticated poultry. In this study we report the detection and genomic characterization of a partridge (Perdix perdix) origin reovirus strain, D1007/2008. The virus was isolated on cell culture from acute pneumonia and infra-orbital sinusitis. The 23,497 nucleotide long genome sequence was obtained by combined use of semiconductor and capillary sequencing. Sequence and phylogenetic analyses showed that the partridge reovirus strain was related to orthoreoviruses of gallinaceous birds. In fact, five (λB, λC, µB, σC, σNS) and one (σB) out of 10 genes clustered definitely with turkey or chicken origin orthoreoviruses, respectively, whereas in the λA, µA, µNS and σA phylogenies a more distant genetic relationship was observed. Our data indicate that the identified reovirus strain is composed of a mixture of chicken and turkey orthoreovirus related alleles. This finding implies that partridges may serve as natural reservoirs for orthoreoviruses of domesticated poultry.


Assuntos
Galliformes/virologia , Orthoreovirus/classificação , Orthoreovirus/genética , Filogenia , Vírus Reordenados/classificação , Vírus Reordenados/genética , Infecções por Reoviridae/veterinária , Animais , Genes Virais , Genoma Viral , Genótipo , Orthoreovirus/isolamento & purificação , Análise de Sequência de DNA , Cultura de Vírus
6.
Sci Rep ; 6: 36960, 2016 11 10.
Artigo em Inglês | MEDLINE | ID: mdl-27830770

RESUMO

The near complete genome sequences of ten field avian orthoreovirus (ARV) strains collected from young chicken between 2002 and 2011 in Hungary have been determined in order to explore the genetic diversity and evolutionary mechanisms affecting ARVs in this region. Sequence analyses and phylogenetic calculations revealed similar geographic distribution and distinct evolution in case of eight studied strains that were closely related to the recently described Hungarian strain T1781. The remaining two strains showed the highest similarity with the US origin AVS-B. The topology of the phylogenetic trees of certain segments was affected by several potential homologous reassortment events between strains of Hungarian, Chinese and US origin. Analyzing the µB gene a possible heterologous reassortment event was identified in three Hungarian strains. Recombination events were detected in as much as a dozen cases implying that beside point mutations and reassorment this mechanism also plays an important role in the diversification of ARVs. All these mechanisms in concert may explain the reduced effectiveness of immunization using commercial vaccine strains.


Assuntos
Evolução Molecular , Rearranjo Gênico , Variação Genética , Orthoreovirus Aviário/genética , Recombinação Genética , Animais , Galinhas , Genoma Viral , Hungria , Orthoreovirus Aviário/classificação , Orthoreovirus Aviário/isolamento & purificação , Filogeografia , Análise de Sequência de DNA
7.
Genome Announc ; 3(6)2015 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-26586882

RESUMO

We have investigated the genomic properties of three turkey reovirus strains-19831M09, D1246, and D1104-isolated in Hungary in 2009. Sequence identity values and phylogenetic calculations indicated genetic conservativeness among the studied Hungarian strains and a close relationship with strains isolated in the United States.

8.
Infect Genet Evol ; 28: 55-7, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25219343

RESUMO

In order to explore the genetic relatedness and evolution of 'classical' and 'novel' waterfowl origin reoviruses (WRV) isolated in different years and continents, and filling up our lacking knowledge about the European WRV strains, the complete genomic sequence of two French isolates causing the 'classical' type of reovirus infection of Muscovy ducks had been determined. Based on the genome organization and the encoded proteins the two isolates could be referred as classical type strains. Sequence comparison showed that the two strains were most closely related to each other and belong to the same monophyletic group of European and Asian WRV strains. Phylogeny of the appropriate segments revealed potential reassortment events between waterfowl and chicken origin, and 'classical' and 'novel' and European and Chinese WRV strains. Our results point out a complex way of viral evolution regarding the origin and biological properties of the WRVs.


Assuntos
Doenças das Aves/virologia , Genoma Viral , Orthoreovirus Aviário/genética , Infecções por Reoviridae/veterinária , Animais , Patos , Orthoreovirus Aviário/classificação , Filogenia , RNA Viral
9.
J Clin Virol ; 55(2): 140-6, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22841750

RESUMO

BACKGROUND: The availability of rotavirus vaccines has resulted in an intensification of post vaccine strain surveillance efforts worldwide to gain information on the impact of vaccines on prevalence of circulating rotavirus strains. OBJECTIVES: In this study, the distribution of human rotavirus G and P types in Hungary is reported. In addition, the VP4 and VP7 genes of G1P[8] strains were sequenced to monitor if vaccine-derived strains were introduced and/or some strains/lineages were selected against. STUDY DESIGN: The study was conducted in 8 geographic areas of Hungary between 2007 and 2011. Rotavirus positive stool samples were collected from diarrheic patients mostly <5 years of age. Viral RNA was amplified by multiplex genotyping RT-PCR assay, targeting the medically most important G and P types. When needed, sequencing of the VP7 and VP4 genes was performed. RESULTS: In total, 2380 strains were genotyped. During the 5-year surveillance we observed the dominating prevalence of genotype G1P[8] (44.87%) strains, followed by G4P[8] (23.4%), G2P[4] (14.75%) and G9P[8] (6.81%) genotypes. Uncommon strains were identified in a low percentage of samples (4.12%). Phylogenetic analysis of 318 G1P[8] strains identified 55 strains similar to the Rotarix strain (nt sequence identities; VP7, up to 97.9%; VP4, up to 98.5%) although their vaccine origin was unlikely. CONCLUSIONS: Current vaccines would have protected against the majority of identified rotavirus genotypes. A better understanding of the potential long-term effect of vaccine use on epidemiology and evolutionary dynamics of co-circulating wild type strains requires continuous strain surveillance.


Assuntos
Infecções por Rotavirus/epidemiologia , Infecções por Rotavirus/virologia , Vacinas contra Rotavirus/imunologia , Rotavirus/classificação , Rotavirus/isolamento & purificação , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Antígenos Virais/genética , Proteínas do Capsídeo/genética , Criança , Pré-Escolar , Fezes/virologia , Feminino , Genótipo , Humanos , Hungria/epidemiologia , Lactente , Masculino , Pessoa de Meia-Idade , Epidemiologia Molecular , Reação em Cadeia da Polimerase Multiplex , RNA Viral/genética , Rotavirus/genética , Vacinas contra Rotavirus/administração & dosagem , Vacinas contra Rotavirus/genética , Análise de Sequência de DNA , Adulto Jovem
10.
Infect Genet Evol ; 10(7): 1140-4, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20471499

RESUMO

The full-length genome of a rare human G8P[14] rotavirus strain, BP1062/04, identified during a surveillance study in Hungary was determined and analyzed. This strain showed a G8-P[14]-I2-R2-C2-M2-A11-N2-T6-E2-H3 genomic constellation. Phylogenetic analysis of each genome segment revealed common origins with selected animal and zoonotic human strains. The closest relatedness was seen with suspect zoonotic Hungarian G6P[14] strains in the NSP1 and NSP3 gene phylogeny, with ovine strains in the VP1, VP2, NSP4 gene phylogeny, and with bovine strains in the NSP5 gene phylogeny. The outer capsid VP7 and VP4 genes could not be derived from cognate genes of any known human or animal G8P[14] strains. The remaining genes, NSP2, VP3 and VP6, gave no definite clues to the host origin, although each was clearly different from true human strains. Altogether, our findings suggest that strain BP1062/04 represents an example of a direct zoonotic transmission event.


Assuntos
Rotavirus/classificação , Rotavirus/genética , Animais , Bovinos , Pré-Escolar , Feminino , Genoma Viral , Humanos , Hungria/epidemiologia , Filogenia , Vigilância da População , Vírus Reordenados , Infecções por Rotavirus/epidemiologia , Infecções por Rotavirus/virologia
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