RESUMO
Arsenic is one of the most hazardous substances in the environment known to cause toxicity in multiple organs. Cell adhesion, morphological alterations, cell proliferation, terminal deoxyuridine triphosphate nick-end labeling (TUNEL) and caspase-3/CPP32 fluorometric protease assay were important biomarkers to assess apoptosis in cells. This study aimed to evaluate arsenic-induced apoptosis in the hepatocytes of rat and its protective efficacy with coadministration of ascorbic acid (AA) and Pleurotus florida lectin (PFL) individually. Results of the present study also showed that arsenic caused cytotoxicity by elevating morphological alterations, TUNEL-positive nuclei, caspase-3 activity and DNA damage and reducing cell adhesion and cell proliferation in a time-dependent manner. The apoptosis in hepatocytes was reverted to normal value after coadministration of mushroom lectin in arsenic-exposed rat. The study provided significant evidence that PFL has antiapoptotic property against arsenic-induced toxicity. The beneficial effect of PFL was proportional to its duration of exposure. Retard activities of superoxide dismutase and catalase, enhanced lipid peroxidation as well as protein carbonyl in erythrocytes caused by arsenic could also be maintained toward normalcy by supplementation of AA and PFL. These antioxidative effects were exhibited in a time-dependant manner. In rat, treatment with AA and PFL prevented alteration of plasma enzyme activities caused by arsenic. The results concluded that treatment with PFL has significant role in protecting animals from arsenic-induced erythrocytic damage. This finding might be of therapeutic benefit in people suffering from chronic exposure to arsenic from natural sources, a global problem especially relevant to millions of people on the Indian subcontinent.
Assuntos
Arsênio/toxicidade , Eritrócitos/efeitos dos fármacos , Lectinas/farmacologia , Fígado/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Pleurotus/química , Animais , Apoptose/efeitos dos fármacos , Arsênio/sangue , Adesão Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Hepatócitos , Marcação In Situ das Extremidades Cortadas , Lectinas/química , Masculino , Oxirredutases/metabolismo , Fagocitose/efeitos dos fármacos , Ratos , Ratos WistarRESUMO
The present study was undertaken to investigate the protective effect of Pleurotus florida lectin (PFL) against arsenic-induced cytotoxicity and oxidative damages in freshly isolated splenocytes of rodents. Our finding indicated that arsenic caused reduction in cell adhesion, morphological alterations, cell proliferation, nitro blue tetrazolium (NBT) index, superoxide dismutase (SOD) activity, catalase (CAT) activity and relative mRNA expression of SOD2 in relation to housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and increased production of nitric oxide (NO), protein carbonyl (PC) and lipid peroxidation levels (LPO) assembled to play key factors for cytotoxicity and oxidative stress. PFL normalized cellular damages and enhanced SOD production pathway relating to gene expression. Further studies are needed to address effective phytochemicals of the edible mushroom and their mechanism.
Assuntos
Produtos Biológicos/farmacologia , Proteínas Fúngicas/farmacologia , Lectinas/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Pleurotus/química , Substâncias Protetoras/farmacologia , Animais , Arsênio/toxicidade , Adesão Celular/efeitos dos fármacos , Forma Celular/efeitos dos fármacos , Células Cultivadas , Fragmentação do DNA/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Óxido Nítrico/análise , Óxido Nítrico/metabolismo , Oxirredutases/análise , Oxirredutases/metabolismo , Ratos , Ratos Wistar , Baço/citologiaRESUMO
Arsenic is ubiquitously found metalloid that commonly contaminates drinking water and agricultural food. To minimise the ecotoxicological effect of arsenic in the environment, it is important to ameliorate the deleterious effects on human and animal health. We investigated the effects of arsenic on cattle by estimating arsenic concentration in biological samples of cattle that consumed contaminated drinking water and feedstuffs directly or indirectly. We have selected arsenic prone village that is Ghentugachi, Nadia district, West Bengal, India, along with arsenic safe control village, Akna in Hoogli district, West Bengal, India. It is found that arsenic is deposited highly in blood, urine and faeces. Agricultural field is contaminated through cattle urine, hair, faeces, cow dung cakes and farmyard manure. Bioconcentration factor and biotransfer factor are two important biomarkers to assess the subclinical toxicity in cattle, as they do not exhibit clinical manifestation like human beings.
Assuntos
Intoxicação por Arsênico/veterinária , Água Potável/química , Exposição Ambiental/análise , Poluentes Químicos da Água/análise , Ração Animal/análise , Animais , Arsênio/análise , Arsênio/sangue , Arsênio/urina , Bovinos , Fezes/química , PoaceaeRESUMO
Arsenicosis caused due to drinking of arsenic contaminated ground water is a major environmental health hazard throughout the world. We evaluated the ecotoxicological effect of arsenic on chicken and duck in an arsenic endemic zone. The concentration of arsenic was higher in chicken and duck feed and their by-products than that in the respective samples of control area. Arsenic concentration in the eggs of both chicken and duck was higher than that in the respective samples of control area. Thus, we concluded that arsenic enters into food chain through the intake of contaminated eggs. Furthermore, adverse health effect of arsenic on avian population is due to the alteration in haematobiochemical indices.
Assuntos
Intoxicação por Arsênico/veterinária , Doenças das Aves Domésticas/induzido quimicamente , Alanina Transaminase/sangue , Animais , Arsênio/análise , Intoxicação por Arsênico/sangue , Intoxicação por Arsênico/epidemiologia , Aspartato Aminotransferases/sangue , Galinhas/metabolismo , Patos/metabolismo , Ovos/análise , Exposição Ambiental/efeitos adversos , Exposição Ambiental/estatística & dados numéricos , Plumas/química , Hematócrito/veterinária , Hemoglobinas/análise , Índia/epidemiologia , Doenças das Aves Domésticas/sangue , Doenças das Aves Domésticas/epidemiologiaRESUMO
In the realm of nanomaterial research, copper telluride and cobalt telluride have individually attracted considerable attention owing to their unique properties and potential applications. However, there exists a notable gap in the literature when it comes to the exploration of composite materials derived from these elements. From this point of view, a ternary CuCoTe nanocomposite was prepared using the microwave synthesis method. Various characterizations were performed by varying the power and irradiation time. X-Ray diffraction study and transmission electron microscopy analysis confirmed the polycrystalline nature of the material with Cu2Te and CoTe hexagonal phases. Field emission scanning electron microscopy images reveal nanoparticle-like morphology, which remains unchanged even when the time of irradiation increases. In addition, the nanoparticle size of the material lies in the range of 30-39 nm. The differential scanning calorimetry inferred various exothermic and endothermic peaks. Meanwhile, the optical analysis from the UV-visible study shows the red-shifted absorbance, enabling the material for semiconductor and photovoltaic devices. Furthermore, the optical bandgap of the material varies in the range from 2.45 to 3.61 eV, which reveals the tuneable bandgap desiring the material for various optoelectronic applications. The frequency-temperature-dependent dielectric study gives results for dielectric parameters, conductivity, and impedance behaviour. The material's dielectric constant, dielectric loss, and AC conductivity enhance with the increase in temperature. This behaviour of the material broadens the area of applicability in energy storage devices.
RESUMO
The value of combining hybridization and mutagenesis in sesame was examined to determine if treating hybrid sesame plant material with mutagens generated greater genetic variability in four key productivity traits than either the separate hybridization or mutation of plant material. In a randomized block design with three replications, six F2M2 varieties, three F2varieties, and three parental varieties were assessed at Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India. The plant characteristics height, number of seed capsules per plant, and seed yield per plant had greater variability in the F2M2 generation than their respective controls (F2), however, the number of primary branches per plant varied less than in the control population. The chances for trait selection to be operative were high for all the characteristics examined except the number of primary branches per plant, as indicated by heritability estimates. Increases in the mean and variability of the characteristics examined indicted a greater incidence of beneficial mutations and the breakdown of undesirable linkages with increased recombination. At both phenotypic and genotypic levels strong positive correlations between both primary branch number and capsule number with seed yield suggest that these traits are important for indirect improvement in sesame seed yield. As a result of the association analysis, sesame seed yield and its component traits improved significantly, which may be attributed to the independent polygenic mutations and enlarged recombination of the polygenes controlling the examined characteristics. Compared to the corresponding control treatment or to one cycle of mutagenic treatment, two cycles of mutagenic treatment resulted in increased variability, higher transgressive segregates, PTS mean and average transgression for sesame seed yield. These findings highlight the value of implementing two EMS treatment cycles to generate improved sesame lines. Furthermore, the extra variability created through hybridization may have potential in subsequent breeding research and improved seed yield segregants may be further advanced to develop ever-superior sesame varieties.
Assuntos
Sesamum , Sesamum/genética , Melhoramento Vegetal , Fenótipo , Genótipo , MutagêneseRESUMO
The present study was planned to investigate the effect of arsenic in rats on several biochemical indices of oxidative stress. Rats were exposed to arsenite in drinking water for upto 12 weeks. Chronic exposure to arsenic for a period of 12 weeks significantly (p < 0.05) increased arsenic burden in blood, liver, and kidney. Several intrinsic antioxidant defenses were activated after a 4-week exposure to arsenic. Some remained elevated, but others became depressed over a longer exposure period. Alterations in most of the biochemical variables reached statistical significant (p < 0.05). Arsenic significantly (p < 0.01) reduced mRNA expression of the superoxide dismutase 2 (SOD2) gene with respect to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene. These observations indicated that prolong exposure to arsenic causes induction of oxidative stress and biochemical alterations.
Assuntos
Arsênio/toxicidade , Eritrócitos/metabolismo , Rim/metabolismo , Fígado/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Animais , Arsênio/farmacocinética , Peso Corporal/efeitos dos fármacos , Catalase/metabolismo , Eritrócitos/efeitos dos fármacos , Eritrócitos/enzimologia , Expressão Gênica/efeitos dos fármacos , Rim/efeitos dos fármacos , Rim/enzimologia , Fígado/efeitos dos fármacos , Fígado/enzimologia , Masculino , Óxido Nítrico/metabolismo , Fagocitose/efeitos dos fármacos , Carbonilação Proteica/efeitos dos fármacos , Ratos , Ratos Wistar , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismoRESUMO
Chalcogenide thin films have been investigated and explored in the last several decades to widen their use in optical, electronic, and optoelectronic device sectors. The phenomenon corresponding to different induced stimuli effects, doping foreign elements is the most productive and efficient way to improve their structural ability, optical characteristics, and electronic approaches. Based on that, metal doping has an enormous impact on the aspects and understanding of the mechanism inside the matrix. This review is mainly based on metal-doped chalcogenide thin films, their effect on various properties of the host materials, and several applications based on that. Thin films doped primarily with bismuth (Bi), antimony (Sb), silver (Ag), tin (Sn), and copper (Cu) were analyzed and discussed. Progress in understanding their structure, bonding, and properties within the matrix was also discussed. This paper also describes the importance and developments of these metal-doped thin films, their physicochemical aspects, and their applications in optoelectronic devices. Different potential applications of these metal-doped chalcogenide thin films in manufacturing technology-based optoelectronic devices, namely sensors, waveguides, switching devices, batteries, optical memories, etc., are also highlighted.
RESUMO
Arsenic is a ubiquitously found metalloid that commonly contaminates drinking water and agricultural food. To understand the ecotoxicological effects of arsenic in environment, it is essential to ameliorate the deleterious effects on human and animal health, particularly on the immune response. We investigated the effects of inorganic arsenic (iAs) on the immune response of chicken splenocytes. Both 1 and 10 mM concentrations of sodium arsenite treatment significantly reduced (P<0.001) splenocyte proliferation and phagocytic activity compared to concanavalin A (ConA) stimulated cells at 24, 48 and 72 h of incubation. Nitrous oxide (NO) production was significantly higher (P<0.001) at 24 h and subsequently declined in the higher dose group, while there was a gradual decline from 24 to 72 h in the lower dose group. Comparison of two different concentration of arsenic treatment also revealed time dependent differences. Relative quantification of expression of IFNγ and IL2 revealed that both genes were significantly down regulated (P<0.001) at both concentrations at each time point. iNOS gene was rapidly down regulated in splenocytes at 24 h at the high doses of As treated splenocyte, a gradual decreasing trend at low doses. Down regulation of IL-2 gene expression in response to As was further evidenced by a significant reduction (P<0.001) in the release of IL-2 into the splenocyte culture medium. We suggest that arsenic, a potent immunotoxic agent, modulates non-specific immune responses and alters the expression of cytokines in a dose and time dependent manner.
Assuntos
Arsenitos/farmacologia , Galinhas/genética , Galinhas/imunologia , Citocinas/genética , Perfilação da Expressão Gênica , Compostos de Sódio/farmacologia , Baço/citologia , Baço/imunologia , Animais , Citocinas/metabolismo , Interferon gama/genética , Interferon gama/metabolismo , Interleucina-2/biossíntese , Interleucina-2/genética , Óxido Nítrico Sintase Tipo II/genética , Óxido Nítrico Sintase Tipo II/metabolismo , Baço/efeitos dos fármacos , Baço/enzimologia , Transcrição Gênica/efeitos dos fármacosRESUMO
The present work demonstrates the impact of thermal annealing on the structural, linear, and non-linear optical characteristics of thermally evaporated BixIn35-xSe65 (x = 0, 5, 10, 15 at%) thin films. The prominent crystalline phases have been developed for all annealed films at 450 °C whereas the films remain amorphous at 350 °C annealing. The XRD and Raman analysis showed the phase transformation of Bi-doped films and new Bi2Se3 phases developed upon annealing at 450 °C. The phase transformation induced change increased the linear and nonlinear properties with great extent as seen from the UV-visible optical studies. The direct and indirect optical bandgaps decreased with annealing temperature and also with Bi % content due to the formation of surface dangling bonds near the crystallite sites. The static linear refractive index and high-frequency dielectric constants were increased with annealing. The third-order non-linear susceptibility and non-linear refractive index were found to be greatly influenced by annealing temperature and increased with bismuth content. The FESEM micrographs also showed the phase transformation and EDX analysis showed the composition. The results obtained from the materials showed the potentiality to be useful for photovoltaic and optoelectronic applications.
RESUMO
Microdroplet impact-induced spray ionization (MISI) is demonstrated involving the impact of microdroplets produced from a paper and their impact on another, leading to the ionization of analytes deposited on the latter. This cascaded process is more advantageous in comparison to standard spray ionization as it performs reactions and ionization simultaneously in the absence of high voltage directly applied on the sample. In MISI, we apply direct current (DC) potential only to the terminal paper, used as the primary ion source. Charge transfer due to microdroplet/ion deposition on the flowing analyte solution on the second surface generates secondary charged microdroplets from it carrying the analytes, which ionize and get detected by a mass spectrometer. In this way, up to three cascaded spray sources could be assembled in series. We show the detection of small molecules and proteins in such ionization events. MISI provides a method to understand chemical reactions by droplet impact. The C-C bond formation reactions catalyzed by palladium and alkali metal ion encapsulation using crown ether were studied as our model reactions. To demonstrate the application of our ion source in a bioanalytical context, we studied the noninvasive in situ discrimination of bacteria samples under ambient conditions.
Assuntos
Bactérias/química , Lipídeos/análise , Espectrometria de Massas por Ionização por Electrospray/métodos , Anisóis/química , Bacillus subtilis/química , Carbono/química , Catálise , Escherichia coli/química , Lipídeos/química , Pseudomonas putida/química , Espectrometria de Massas por Ionização por Electrospray/instrumentaçãoRESUMO
Generation of current or potential at nanostructures using appropriate stimuli is one of the futuristic methods of energy generation. We developed an ambient soft ionization method for mass spectrometry using 2D-MoS2, termed streaming ionization, which eliminates the use of traditional energy sources needed for ion formation. The ionic dissociation-induced electrokinetic effect at the liquid-solid interface is the reason for energy generation. We report the highest figure of merit of current generation of 1.3 A/m2 by flowing protic solvents at 22 µL/min over a 1 × 1 mm2 surface coated with 2D-MoS2, which is adequate to produce continuous ionization of an array of analytes, making mass spectrometry possible. Weakly bound ion clusters and uric acid in urine have been detected. Further, the methodology was used as a self-energized breath alcohol sensor capable of detecting 3% alcohol in the breath.
Assuntos
Molibdênio , Nanoestruturas , Dissulfetos , Espectrometria de MassasRESUMO
This study aimed to determine the hemato-biochemical picture and blood oxidative stress in zebu cattle in an arsenic-contaminated zone. Significant decline in total erythrocyte count, packed cell volume, and total plasma protein was observed in cattle of that area in comparison to uncontaminated zone. There was significant elevation of plasma enzyme activities of both alanine aminotransaminase and aspertate aminotransaminase. Increased corpuscular osmotic fragility also proved to be a mechanism for deviation from normal functioning of erythrocytes. Cattle in the affected zone showed a significantly higher arsenic burden in blood. Those animals further showed decreased superoxide dismutase, catalase activities of erythrocytes, and plasma nitrite level, but increased lipid peroxidation and protein carbonyl level. Our finding concluded that cattle of the arsenic-contaminated zone is suffering from a subclinical form of arsenic toxicity, which is proved through altered hemato-biochemical indices and a certain extent of oxidative stress with higher arsenic concentration in blood.
Assuntos
Intoxicação por Arsênico/sangue , Arsênio/toxicidade , Doenças dos Bovinos/sangue , Estresse Oxidativo/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Alanina Transaminase/sangue , Animais , Arsênio/análise , Arsênio/sangue , Intoxicação por Arsênico/enzimologia , Intoxicação por Arsênico/metabolismo , Intoxicação por Arsênico/veterinária , Aspartato Aminotransferases/sangue , Proteínas Sanguíneas/análise , Bovinos , Doenças dos Bovinos/enzimologia , Doenças dos Bovinos/metabolismo , Ingestão de Líquidos , Eritrócitos/enzimologia , Índia , Peroxidação de Lipídeos/efeitos dos fármacos , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/sangueRESUMO
Chronic arsenicosis is a major environmental health hazard throughout the world, including India. Animals and human beings are affected due to drinking of arsenic contaminated ground water, due to natural mineral deposits, arsenical pesticides or improperly disposed arsenical chemicals. Arsenic causes cancer with production of free radicals and reactive oxygen species (ROS) that are neutralized by an elaborate antioxidant defense system consisting of enzymes and numerous non-enzymatic antioxidants. Dietary antioxidant supplements are useful to counteract the carcinogenesis effects of arsenic. Oyster mushroom lectins can be regarded as ingredients of popular foods with biopharmaceutical properties. A variety of compounds have been isolated from mushrooms, which include polysaccharides and polysaccharopeptides with immune-enhancing effects. Lectins are beneficial in reducing arsenic toxicity due to anticarcinogenetic roles and may have therapeutic application in people suffering from chronic exposure to arsenic from natural sources, a global problem that is especially relevant to millions of people on the Indian subcontinent.
Assuntos
Agaricales/química , Intoxicação por Arsênico/complicações , Carcinogênese/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Lectinas/uso terapêutico , Estresse Oxidativo/efeitos dos fármacos , Animais , Intoxicação por Arsênico/fisiopatologia , Carcinogênese/patologia , HumanosRESUMO
Thirty goats were selected randomly from a village of Nadia district, West Bengal according to the previous reports of human being suffering from chronic arsenicosis. Environmental samples viz. drinking water, rice plants and grass used for goat and biological samples viz. blood, urine, faeces, hair and meat were collected to evaluate the arsenic status. It was found that arsenic concentration in both environmental and biological samples was significantly (p<0.01) higher rather than respective samples on control zone. Bio-concentration factor (BCF) and bio-transfer factor (BTF) are indicated to evaluate the subclinical toxicity in goat as they do not exhibit clinical manifestation like human beings.
Assuntos
Intoxicação por Arsênico/veterinária , Arsênio/análise , Poluentes Ambientais/análise , Doenças das Cabras/induzido quimicamente , Animais , Arsênio/sangue , Arsênio/urina , Intoxicação por Arsênico/etiologia , Intoxicação por Arsênico/metabolismo , Carga Corporal (Radioterapia) , Água Potável/química , Monitoramento Ambiental , Poluentes Ambientais/sangue , Poluentes Ambientais/urina , Fezes/química , Cadeia Alimentar , Contaminação de Alimentos , Doenças das Cabras/metabolismo , Cabras , Cabelo/química , Humanos , Índia , Carne/análise , Oryza/química , Poaceae/química , Medição de RiscoRESUMO
Chronic arsenic exposure results in toxicity in humans and causes many toxicologic manifestations. Apoptosis was measured by cell adhesion, morphologic alterations, cell proliferation, terminal deoxyuridine triphosphate nick-end labeling (TUNEL), and caspase-3/CPP32 fluorometric protease assay. Results of the present study suggested that arsenic administration in rats caused apoptosis by elevating morphologic alterations, TUNEL-positive nuclei, caspase-3 activity, and DNA damage and by reducing cell adhesion and cell proliferation in a time-dependent manner. The apoptosis in renal cells of arsenic-exposed rats reverted to normal values after coadministration of mushroom lectin. This study provided significant evidence that Pleurotus florida lectin has an antiapoptotic property by protecting from arsenic-induced toxicity. The beneficial effect of Pleurotus florida lectin was proportional to its duration of exposure. This finding might be of therapeutic benefit in people suffering from chronic exposure to arsenic from natural sources, a global problem that is especially relevant to millions of people on the Indian subcontinent.
Assuntos
Apoptose/efeitos dos fármacos , Intoxicação por Arsênico/prevenção & controle , Arsênio/toxicidade , Rim/efeitos dos fármacos , Lectinas/farmacologia , Lectinas/uso terapêutico , Pleurotus , Animais , Arsênio/farmacologia , Caspase 3/metabolismo , Adesão Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Doença Crônica , Dano ao DNA/efeitos dos fármacos , Relação Dose-Resposta a Droga , Rim/metabolismo , Rim/patologia , Masculino , Modelos Animais , Ratos , Ratos WistarRESUMO
Natural contamination of arsenic in ground water is a major health problem throughout the World. It is one of the most hazardous substances in the environment known to cause toxicity in multiple organs via oxidative stress. The molecular basis for arsenic toxicity involves direct or indirect damage to protein, lipid and DNA. Various studies have focused on the possible toxic effects of arsenic on membrane components and its correlation with oxidative damage. The present study was aimed to mitigation of arsenic induced hepatic oxidative stress by dietary modulation using of mushroom lectin in rats. Animals were divided into four groups; the first group was used as control. Groups 2, 3 and 4 were arsenic (20 ppm) exposed through drinking water, arsenic exposed plus oral ascorbic acid (25 mg/kg body weight) and arsenic exposed plus oral mushroom lectin (150 mg/kg body weight) respectively for a period of 12 weeks. We observed significant alterations in the antioxidant enzymes, oxidative stress intermediates and SOD(2) gene expression profile on arsenic exposure. These alterations were restored by co-administration of Pleurotus florida lectin which was as potent as standard antioxidant viz. ascorbic acid. The findings of the experiment suggested that P. florida lectin has capability of modulating arsenic mediated toxic effects and could be helpful in ameliorating them.
Assuntos
Antioxidantes/farmacologia , Arsenitos/toxicidade , Lectinas/farmacologia , Fígado/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Pleurotus/química , Compostos de Sódio/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Antioxidantes/isolamento & purificação , Arsenitos/farmacocinética , Lectinas/isolamento & purificação , Peróxidos Lipídicos/metabolismo , Fígado/enzimologia , Fígado/metabolismo , Fígado/patologia , Masculino , Óxido Nítrico/metabolismo , Tamanho do Órgão/efeitos dos fármacos , Carbonilação Proteica , Ratos , Ratos Wistar , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Compostos de Sódio/farmacocinética , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismo , Fatores de Tempo , Transcrição Gênica , Poluentes Químicos da Água/farmacocinéticaRESUMO
This study was accomplished to exemplify the possible protective role of ascorbic acid and mushroom lectin against arsenic-induced cytotoxicity and impairment of superoxide dismutase (SOD) production pathway in hepatocytes of rat. Hepatocytes were isolated from rat and treated with sodium arsenite (AS), arsenic plus ascorbic acid (AS + AA) and arsenic plus mushroom lectin (AS + ML). A placebo control was also included. Arsenic treatment resulted in the depletion of cell proliferation, phagocytic activity (nitro blue tetrazolium index) and superoxide dismutase (SOD) activity, relative mRNA expression of superoxide dismutase 2 (SOD(2)) and enhanced production of nitric oxide (NO). Ascorbic acid, a standard antioxidant, could normalize cellular perturbation and SOD production pathway relating to gene expression, whereas partially purified Pleurotus florida lectin (PFL), an edible mushroom containing protein complex, maintained cellular activity and prevented stress by normalizing phagocytic (NBT index) and SOD activities vis-à-vis relative gene expression. It could further defend NO production of hepatocytes. Mushroom lectin strongly prevented sodium arsenite-induced damage of SOD production pathway in hepatocytes, and its effect was also comparable to a standard antioxidant, i.e. ascorbic acid.
Assuntos
Arsenitos/toxicidade , Inibidores Enzimáticos/toxicidade , Hepatócitos/efeitos dos fármacos , Lectinas de Plantas/farmacologia , Pleurotus/química , Compostos de Sódio/toxicidade , Superóxido Dismutase/antagonistas & inibidores , Animais , Antioxidantes/farmacologia , Arsenitos/antagonistas & inibidores , Ácido Ascórbico/farmacologia , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Antagonismo de Drogas , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Hepatócitos/enzimologia , Masculino , Fagocitose/efeitos dos fármacos , Extratos Vegetais/farmacologia , RNA Mensageiro/metabolismo , Ratos , Ratos Wistar , Compostos de Sódio/antagonistas & inibidores , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismoRESUMO
Acute and chronic arsenic exposure result in toxicity both in human and animal beings and cause many hepatic and renal manifestations. The present study stated that mushroom lectin prevents arsenic-induced apoptosis. Apoptosis was measured by morphological alterations, cell proliferation index (CPI), phagocytic activity (nitro blue tetrazolium index; NBT), nitric oxide (NO) production, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, DNA fragmentation and caspase-3 activity. Arsenic exposure at 5 µM in the form of sodium arsenite resulted in significant elevation of deformed cells, NO production, TUNEL stained nuclei of hepatocytes, DNA fragmentation and caspase-3 activity. But the CPI and NBT index were significantly declined in arsenic-treated hepatocytes. The beneficial effect of mushroom lectin at 10 µg/mL, 20 µg/mL and 50 µg/mL) showed increased CPI and phagocytic activity. Mushroom lectin at those concentrations reduced deformed cells, NO production, DNA fragmentation and caspase-3 activity of hepatocytes. But significant better protection was observed in 50 µg/mL mushroom lectin-treated hepatocytes. This finding may be of therapeutic benefit in people suffering from chronic arsenic exposure.