A drosophila genetic resource of mutants to study mechanisms underlying human genetic diseases.

Invertebrate model systems are powerful tools for studying human disease owing to their genetic tractability and ease of screening. We conducted a mosaic genetic screen of lethal mutations on the Drosophila X chromosome to identify genes required for the development, function, and maintenance of the nervous system. We identified 165 genes, most of whose function has not been studied in vivo. In parallel, we investigated rare variant alleles in 1,929 human exomes from families with unsolved Mendelian disease. Genes that are essential in flies and have multiple human homologs were found to be likely to be associated with human diseases. Merging the human data sets with the fly genes allowed us to identify disease-associated mutations in six families and to provide insights into microcephaly associated with brain dysgenesis. This bidirectional synergism between fly genetics and human genomics facilitates the functional annotation of evolutionarily conserved genes involved in human health.

Estimation of genetic parameters for the tick and hemoparasite burden in Angus cattle.

The study was conducted with the objective of estimating genetic and phenotypic parameters for tick (CRM) and Babesia bigemina (IBBi), Babesia bovis (IBBo), and Anaplasma marginale (IAM) burden in Angus female breed in Brazil. The sample group was composed of Angus females raised in herds located in a region of endemic instability for cattle tick fever in the state of Rio Grande Sul (RS), Brazil. The variance components were estimated using Bayesian inference and Gibbs sampling algorithm, considering a multi-trait animal model. Heritability estimates showed values of low magnitude, ranging from 0.03 (IBBo) to 0.16 (CRM), while repeatability estimates ranged between 0.07 (IBBo) and 0.21 (CRM). Regarding the genetic correlation estimates, the values showed low (-0.01 for IBBo × IAM) to moderate (0.55 between IBBi × IAM) magnitudes. The results indicate that it is possible to use tick count and hemoparasite infection levels as selection criteria, with small genetic gains.

Characterization of the Physical Fitness of Police Officers: A Systematic Review.

Marins, EF, David, GB, and Del Vecchio, FB. Characterization of the physical fitness of police officers: a systematic review. J Strength Cond Res 33(10): 2860-2874, 2019-Physical fitness tests (e.g., aerobic power, muscular endurance, and flexibility tests) are commonly used to assess the ability of police officers to perform work-related tasks. The purpose of this study was to describe, from a systematic literature review, data related to police physical fitness. The research was conducted in 5 electronic databases to search for original studies that measured physical fitness (aerobic and anaerobic capacity, strength, endurance, power, flexibility, agility, and speed) of police officers, as well as the article references. Original studies assessing objective measures of physical fitness in police officers were included, with no date restriction. Fifty-nine articles were included in the review. The studies mostly measured cardiorespiratory fitness indirectly, strength, and muscular endurance, as well as other performance components (body composition, power, flexibility, speed, agility, and anaerobic profile), with police officers generally presenting values similar or above the average of the general population. It can be concluded that intervention studies are needed to promote and incorporate programs related to improvement or maintenance of physical fitness in police officers, which would result in health benefits and specifically improvement in performance of specific tasks of police work. This review provides summary information to assist in the selection of physical fitness tests for police populations. Still, these findings have practical applications for public security agencies and its personnel responsible for the development and implementation of physical programs in policemen population.

Correction: the retromer complex is required for rhodopsin recycling and its loss leads to photoreceptor degeneration.

[This corrects the article DOI: 10.1371/journal.pbio.1001847.].

Large-scale identification of chemically induced mutations in Drosophila melanogaster.

Forward genetic screens using chemical mutagens have been successful in defining the function of thousands of genes in eukaryotic model organisms. The main drawback of this strategy is the time-consuming identification of the molecular lesions causative of the phenotypes of interest. With whole-genome sequencing (WGS), it is now possible to sequence hundreds of strains, but determining which mutations are causative among thousands of polymorphisms remains challenging. We have sequenced 394 mutant strains, generated in a chemical mutagenesis screen, for essential genes on the Drosophila X chromosome and describe strategies to reduce the number of candidate mutations from an average of -3500 to 35 single-nucleotide variants per chromosome. By combining WGS with a rough mapping method based on large duplications, we were able to map 274 (-70%) mutations. We show that these mutations are causative, using small 80-kb duplications that rescue lethality. Hence, our findings demonstrate that combining rough mapping with WGS dramatically expands the toolkit necessary for assigning function to genes.

Drosophila Tempura, a novel protein prenyltransferase α subunit, regulates notch signaling via Rab1 and Rab11.

Vesicular trafficking plays a key role in tuning the activity of Notch signaling. Here, we describe a novel and conserved Rab geranylgeranyltransferase (RabGGT)-α-like subunit that is required for Notch signaling-mediated lateral inhibition and cell fate determination of external sensory organs. This protein is encoded by tempura, and its loss affects the secretion of Scabrous and Delta, two proteins required for proper Notch signaling. We show that Tempura forms a heretofore uncharacterized RabGGT complex that geranylgeranylates Rab1 and Rab11. This geranylgeranylation is required for their proper subcellular localization. A partial dysfunction of Rab1 affects Scabrous and Delta in the secretory pathway. In addition, a partial loss Rab11 affects trafficking of Delta. In summary, Tempura functions as a new geranylgeranyltransferase that regulates the subcellular localization of Rab1 and Rab11, which in turn regulate trafficking of Scabrous and Delta, thereby affecting Notch signaling.

The retromer complex is required for rhodopsin recycling and its loss leads to photoreceptor degeneration.

Rhodopsin mistrafficking can cause photoreceptor (PR) degeneration. Upon light exposure, activated rhodopsin 1 (Rh1) in Drosophila PRs is internalized via endocytosis and degraded in lysosomes. Whether internalized Rh1 can be recycled is unknown. Here, we show that the retromer complex is expressed in PRs where it is required for recycling endocytosed Rh1 upon light stimulation. In the absence of subunits of the retromer, Rh1 is processed in the endolysosomal pathway, leading to a dramatic increase in late endosomes, lysosomes, and light-dependent PR degeneration. Reducing Rh1 endocytosis or Rh1 levels in retromer mutants alleviates PR degeneration. In addition, increasing retromer abundance suppresses degenerative phenotypes of mutations that affect the endolysosomal system. Finally, expressing human Vps26 suppresses PR degeneration in Vps26 mutant PRs. We propose that the retromer plays a conserved role in recycling rhodopsins to maintain PR function and integrity.

HR, ̇V̇O2, and RPE Relationships in an Aquatic Incremental Maximum Test Performed by Young Women.

David, GB, Andrade, LS, Schaun, GZ, and Alberton, CL. HR, V̇O2, and RPE relationships in an aquatic incremental maximum test performed by young women. J Strength Cond Res 31(10): 2852-2858, 2017-The purpose of the study was to verify the relationship between oxygen uptake (V̇O2), heart rate (HR), rate of perceived exertion (RPE), and cadence (CAD) in an aquatic incremental maximum test performed during a water-based stationary running exercise. The study also verified the best adjustments for these relationships (linear or polynomial). Thirteen young college women (mean ± SD: 23.15 ± 1.72 years, 21.43 ± 2.09 body mass index) participated in the study. They performed an aquatic incremental maximum test during a water-based stationary running exercise. The experimental protocol started at an initial CAD of 80 b·min, and it was followed by increases of 10 b·min every minute until exhaustion. V̇O2, HR, and RPE were measured along the test. Linear and polynomial regression analysis were performed to determine the relationship among the percentage of peak V̇O2 (%V̇O2peak), percentage of maximal HR (%HRmax), RPE, and CAD to determine the best adjustment for each analysis (α = 0.05). The polynomial regression showed the best adjustments for all analysis. Data revealed a significant relationship (p < 0.001) between %V̇O2peak and %HRmax (r = 0.858), %HRmax and RPE (r = 0.823), and %V̇O2peak and RPE (r = 0.871). Regarding the relationship between these above-mentioned variables and CAD, all of them were significant (p < 0.001), with r = 0.848 for CAD and %HRmax, r = 0.877 for CAD and %V̇O2peak and r = 0.878 for CAD and RPE. It was concluded that all analyzed variables are associated and their relationships are in a polynomial quadratic form. Based on the findings, instructors may use the positive relationships between %V̇O2peak, %HRmax, and RPE to efficiently prescribe water-based training sessions.

Crag is a GEF for Rab11 required for rhodopsin trafficking and maintenance of adult photoreceptor cells.

Rhodopsins (Rhs) are light sensors, and Rh1 is the major Rh in the Drosophila photoreceptor rhabdomere membrane. Upon photoactivation, a fraction of Rh1 is internalized and degraded, but it remains unclear how the rhabdomeric Rh1 pool is replenished and what molecular players are involved. Here, we show that Crag, a DENN protein, is a guanine nucleotide exchange factor for Rab11 that is required for the homeostasis of Rh1 upon light exposure. The absence of Crag causes a light-induced accumulation of cytoplasmic Rh1, and loss of Crag or Rab11 leads to a similar photoreceptor degeneration in adult flies. Furthermore, the defects associated with loss of Crag can be partially rescued with a constitutive active form of Rab11. We propose that upon light stimulation, Crag is required for trafficking of Rh from the trans-Golgi network to rhabdomere membranes via a Rab11-dependent vesicular transport.

Caffeine Supplementation Effects on Concurrent Training Performance in Resistance-Trained Men: A Double-Blind Placebo-Controlled Crossover Study.

Purpose: The aim of the present study was to investigate the effects of acute caffeine supplementation on the performance during a session of resistance training alone (RT) or in combination with aerobic training (i.e. concurrent training; CT). Method: Fourteen resistance-trained men (23.1 ± 4.2 years) were recruited and performed both RT and CT under three different conditions: control (CONT), placebo (PLA), and caffeine (CAF; 6 mg.kg-1) for a total of six experimental conditions. Results: Both total and per set number of repetitions, and total volume load were lower during CT as compared to RT, irrespective of the supplementation condition (all p < .001), whereas a supplementation main effect was observed for the total number of repetitions (p = .001), the number of repetitions in the first (p = .002) and second sets (p = .001), and total volume load (p = .001). RPE values were higher after the CT sessions than after the RT sessions (p < .001), whereas no differences were observed between supplementation conditions (p = .865). Conclusions: Caffeine supplementation was not sufficient to minimize the acute interference effect on strength performance in a CT session when compared to RT alone. In contrast, caffeine improved strength performance during the first set of both CT and RT, while maintaining a similar RPE between the supplementation conditions. However, the overall effect was small.

Effect of High-Intensity Interval Treadmill Exercise on Subsequent Lower and Upper Limb Strength Performance.

Purpose: The aim of this study was to analyze the acute effect of a treadmill high-intensity interval protocol on subsequent upper body and lower body strength exercise performance. Method: Sixteen young men had their maximal aerobic capacity and one-repetition maximum (1RM) determined and underwent four randomized conditions: a half-squat control session; a bench press control session; a treadmill interval protocol followed by a half-squat experimental session; and a treadmill interval protocol followed by a bench press experimental session. During the control sessions, four sets to failure for each exercise were performed at 80% of 1RM. In the experimental sessions, participants performed eight sprints of 40 s at 100% velocity of maximal oxygen uptake with 20 s of passive interval between them, followed by the same strength exercise protocol of the control sessions. The number of repetitions during each protocol and participants' heart rate (HR),and blood lactate concentration ([lac]) were compared pre and post protocols and exercises. Results: Fewer repetitions were completed in the experimental session compared to the control sessions (p < .001). Moreover, the reduction in number of repetitions performed was more evident in half-squat compared to bench press (p = .018). HR was higher at the end of sessions with the interval protocol for both exercises (p < .001). The [lac] was higher at the end of session with the interval protocol for half-squat (p = .003). Conclusions: These findings suggest that previous high-intensity interval running may impair subsequent strength exercise performance, but the magnitude of the negative effect is greater in the lower limbs.

Impact of Resistance Training Volume on Physical and Perceptual Outcomes of Breast Cancer Survivors Submitted to a Combined Training Program: A Randomized, Single-Blinded Study.

BACKGROUND: To determine the effect of resistance training volume on physical and perceptual outcomes of breast cancer survivors submitted to a combined training program. DESIGN: Randomized single-blinded study. METHODS: Nineteen breast cancer survivor women were randomized to a single-set (SS) or a multiple-set (MS) group. Both groups completed an 8-week combined training intervention in which the SS and MS groups performed 1 and 3 sets per resistance exercise, respectively. The following outcomes were assessed preintervention and postintervention: maximal knee extension dynamic strength (1-repetition maximum), quadriceps muscle thickness, peak oxygen uptake, time to exhaustion, cancer-related fatigue, and quality of life. RESULTS: Both interventions increased knee extension 1-repetition maximum (SS: 29.8% [37.5%]; MS: 19.3% [11.8%]), quadriceps muscle thickness (9.4% [4.1%]; 8.9% [5.9%]), and quality of life (4.3% [6.3%]; 7.9% [9.0%]), with no difference between the groups. However, only MS improved cancer-related fatigue (-2.1% [1.7%]) and time to exhaustion (21.3% [14.9%]), whereas peak oxygen uptake remained unchanged in both groups. CONCLUSIONS: Cancer-related fatigue and time to exhaustion, improved only in the MS group after the intervention. On the other hand, similar knee extension 1-repetition maximum, quadriceps muscle thickness, and quality of life improvements were observed in breast cancer survivors irrespective of the resistance training volume performed.

Cardiorespiratory Parameters Comparison Between Incremental Protocols Performed in Aquatic and Land Environments by Healthy Individuals: A Systematic Review and Meta-Analysis.

BACKGROUND: Physical properties of water cause physiological changes in the immersed human body compared with the land environment. Understanding the magnitude of cardiorespiratory alterations might ensure adequate intensity control during aquatic exercise programs. OBJECTIVE: We aimed to compare the oxygen uptake (VO2), heart rate (HR), and rating of perceived exertion (RPE) parameters during aquatic and land incremental tests. METHODS: Four databases (PubMed, LILACS, EMBASE, and SPORTDiscus) were searched in September 2020. Eligibility criteria included studies in a crossover design comparing aquatic and land incremental tests for healthy individuals with at least one of the following parameters: VO2 (maximal, VO2max; anaerobic threshold, VO2AT), HR (HRmax; HRAT), and RPE (RPEmax; RPEAT). The random-effects meta-analysis included mean difference and 95% confidence interval for VO2 and HR or standardized mean difference for RPE. The Joanna Briggs Institute Critical Appraisal tool was adapted to assess methodological quality. RESULTS: Twenty-eight studies were eligible and included in the meta-analysis. Aquatic protocols showed lower values compared with land for VO2max (- 7.07 mL.kg-1.min-1; - 8.43 to - 5.70; n = 502), VO2AT (- 6.19 mL.kg-1.min-1; - 7.66 to - 4.73; n = 145), HRmax (- 11.71 bpm; - 13.84 to - 9.58; n = 503), and HRAT (- 15.29 bpm; - 19.05 to - 11.53; n = 145). RPEmax (0.01; - 0.16 to 0.18; n = 299) and RPEAT (- 0.67; - 1.35 to 0.02; n = 55) values were similar between aquatic and land protocols. CONCLUSIONS: Our study reinforces the specificity of the environment during incremental tests for prescribing exercises based on physiological parameters as VO2 and HR parameters presented lower values in aquatic protocols than land protocols. Conversely, RPE seems an interchangeable measure of exercise intensity, with similar values during the protocols in both environments. Substantial levels of heterogeneity were present for the VO2max and HRmax meta-analyses, and as such, results should be interpreted with attention. PROTOCOL REGISTRATION: This study was registered in the International Prospective Register of Systematic Reviews (PROSPERO; CRD42020212508).

Short-Term Effects of Land-Based Versus Water-Based Resistance Training Protocols on Post-Exercise Hypotension in Normotensive Men: A Crossover Study.

Considering that water immersion may acutely reduce blood pressure (BP) and that exercise may elicit positive post-exercise hypotension (PEH) responses, we aimed to analyze the presence of PEH in normotensive individuals and compare its magnitude between two resistance training sessions performed in aquatic or land environments. Ten physically active men (23.2 ± 3.1 years) performed the two training protocols in a randomized, counterbalanced fashion. BP measurements were performed for 30 min (at 5 min intervals) both prior to (resting) and after each of the protocols. No differences were observed between protocols at baseline (p > 0.05). Only the water-based resistance training protocol resulted in a systolic BP reduction from 10 to 20 min post-exercise (all p < 0.05) compared to baseline. Compared to the land-based session, systolic BP was lower in the water-based protocol from 10 to 25 min post-exercise (all p < 0.05). On the other hand, diastolic BP showed a similar PEH effect between water and land-based protocols for the entire 30 min post-session period (all p < 0.001). Our results suggest that water-based resistance training holds the potential as a nonpharmacological strategy to lower BP levels following exercise.

High-velocity resistance training mitigates physiological and functional impairments in middle-aged and older adults with and without mobility-limitation.

The aim of the present study was to compare the neuromuscular, morphological, and functional responses to a high-velocity resistance training (HVRT) program between three cohorts: middle-aged adults (40-55 years, n = 18), healthy older adults (> 60 years, n = 18), and mobility-limited older adults (n = 8). Participants were tested before and after a 4-week control period and then assigned to a 12-week HVRT intervention. Investigated outcomes included ultrasound-derived muscle thickness and quality, maximal dynamic strength (1RM), maximal voluntary isometric contraction (MVIC), and muscle activation (sEMG), as well as muscle power and functional performance. After the intervention, quadriceps muscle thickness, 1RM, and sEMG improved in all three groups (all p < 0.05), whereas muscle quality improved only in middle-aged and older participants (p ≤ 0.001), and MVIC only in middle-aged and mobility-limited older adults (p < 0.05). With a few exceptions, peak power improved in all groups from 30-90% 1RM (p < 0.05) both when tested relative to pre-training or post-training 1RM workloads (all p < 0.05). Both mobility-limited older adults and older adults improved their short physical performance battery score (p < 0.05). Chair stand, stair climb, maximal gait speed, and timed up-and-go performance, on the other hand, improved in all three groups (p < 0.05), but no change was observed for habitual gait speed and 6-min walk test performance. Overall, our results demonstrate that a HVRT intervention can build a stronger foundation in middle-aged individuals so that they can better deal with age-related impairments at the same time that it can mitigate already present physiological and functional impairments in older adults with and without mobility-limitation.

High-intensity interval running impairs subsequent upper limb strength performance.

BACKGROUND: This study compared the effect of treadmill running on subsequent upper limb exercise performance in young men. METHODS: Seventeen young men (24.8±5.2 years) completed a: 1) bench press resistance exercise control session; 2) treadmill interval running protocol followed by the bench press session; and 3) treadmill continuous running protocol followed by the bench press session. Four sets of the bench press exercise were performed at 80% of 1RM up to volitional failure. In the interval protocol, eight sprints of 40s at 100% of the velocity of maximal oxygen uptake, with 20s of passive interval between them were performed, whereas in the continuous protocol 30-min of treadmill running at 90% of the heart rate corresponding to second ventilatory threshold was performed. The number of maximal repetitions completed in each set and condition was recorded and compared using a two-way repeated measures ANOVA. RESULTS: The interval protocol (18.7±4.9 repetitions) resulted in a reduction in the number of bench press repetitions compared to the control protocol (21.4±5.4 repetitions) (P=0.002); whereas continuous running did not affect the bench press performance (20.6±4.4 repetitions). The total number of repetitions reduced from set to set in all protocols (P<0.001). CONCLUSIONS: The results evidenced an impairment in the upper limb strength performance after high intensity interval, but not moderate intensity continuous running, which has implication for concurrent training planning and prescription.

Water-based continuous and interval training in older women: Cardiorespiratory and neuromuscular outcomes (WATER study).

The purpose of this study was to investigate the effects of two water-based aerobic programs on cardiorespiratory and neuromuscular outcomes in older women. Forty-one women (60 to 75 years old) volunteered to participate in the study. Participants were randomized into a water-based continuous (CTG; n = 21; 63.9 ± 2.5 years) or an interval (ITG; n = 20; 64.8 ± 3.6 years) aerobic training group. Both training programs were performed for 12 weeks (45-min sessions twice a week), with exercise intensity based on rating of perceived exertion (Borg's RPE 6-20 Scale). Pre and post training assessments of cardiorespiratory and neuromuscular outcomes were performed. Data analyses were conducted using Generalized Estimating Equations and Bonferroni post-hoc test (α = 0.05). After the intervention, the CTG and the ITG displayed similar improvements in time to exhaustion (8% vs. 11%), peak oxygen uptake (9% vs. 7%), maximal dynamic knee extension strength (5% vs. 6%), dynamic muscular endurance of knee extensors (10% vs. 11%), maximal vastus lateralis electromyographic signal amplitude (13% vs. 35%), as well as an increase in muscle thickness (5% vs. 6%) and decrease in muscle echo intensity (-2% vs. -3%) of the quadriceps femoris. In conclusion, older women benefited from water-based exercise training prescribed based on participants' RPE, with both the interval and the continuous training programs resulting in similar increases in the cardiorespiratory and neuromuscular parameters.

Muscular and cardiorespiratory parameters of Brazilian professional futsal players: comparison between top national and regional level athletes

Abstract Aim: To compare muscular and cardiorespiratory parameters between Brazilian professional futsal athletes from different competitive levels (national versus regional). A secondary aim was to explore the correlation of physical fitness variables between both competitive levels. Methods: Twenty-two futsal players (24.7 ± 3.7 years), 11 from a regional team and 11 from a national team, were evaluated with countermovement jump test (lower limbs muscle power), square test (the speed with change direction), RSSA (anaerobic power), and YOYOIR1 (aerobic power). Tests were performed in the middle of the season for both teams. Results: The speed with change direction (p = 0.001) and mean anaerobic power (p = 0.04) were higher for national than the regional players. Sprint performance decreased similarly for national and regional level players (p < 0.001), but the latter had poorer performance (p = 0.044). From the fourth sprint on, sprints for the national level players were slower than the first three, while for the regional level players, each sprint speed was slower than the subsequent. A significant correlation was found between anaerobic power with jump height (r = −0.50; p = 0.01), speed with change direction (r=0.65; p = 0.001) and distance covered in the YOYOIR1 (r = −0.54; p = 0.01). Conclusion: National-level athletes were more agile and presented a higher mean anaerobic power than regional ones. Moreover, national-level athletes presented better performance along with repeated sprint tests, maintaining this performance for a longer time during the test.

Efeitos de um macrociclo de treinamento sobre a aptidão física em atletas de futsal feminino durante uma temporada / Effects of a training macrocycle on the physical fitness in female futsal athletes during a season

O objetivo do presente estudo foi analisar os efeitos de macrociclo de treinamento sobre variáveis de aptidão física em atletas semiprofissionais de futsal feminino. Foram avaliadas quatorze atletas (21,1 ± 4,8 anos) semiprofissionais durante 27 semanas de treinamento, correspondentes a: período pré-competitivo e período competitivo. Foram realizadas avaliações antropométricas (massa corporal, estatura e percentual de gordura corporal), testes de potência de membros inferiores (salto vertical e salto horizontal) e membros superiores (arremesso de medicine ball), de força de membros inferiores (1RM de agachamento e legpress) e de membros superiores (supino) e de velocidade (linear e com troca de direção) antes e após o período pré-competitivo e após a primeira fase do período competitivo. ANOVA para medidas repetidas foi utilizada para comparação das variáveis entre os três momentos de avaliação com post hoc de Bonferroni (α = 0,05). Potência de membros inferiores, velocidade linear e força de membros inferiores e superiores (1RM legpress e supino) apresentaram melhora no desempenho nos três momentos (p < 0,001). Percentual de gordura corporal, potência de membros superiores, força de membros inferiores (1RM agachamento) (p < 0,001) e velocidade com troca de direção (p = 0,002) melhoraram o desempenho do momento pré para o momento pós período pré-competitivo e mantiveram este desempenho no momento pós período competitivo. A massa corporal não apresentou diferença significativa entre os momentos. Conclui-se que, os resultados encontrados indicam que as atletas responderam positivamente quanto às variáveis de aptidão física, comprovando a eficácia do treinamento. Ainda, destaca-se a importância do planejamento dos treinos para o sucesso da equipe. (AU)

The aim of the present study was to analyze the macrocycle training effects on physical fitness variables in semiprofessional female futsal athletes. Fourteen semiprofessional athletes (21.1 ± 4.8 years) were measured during 27 weeks of training, corresponding to: precompetitive period and competitive period. The anthropometric variables (body mass, height and body fat percentage), lower limb (vertical and horizontal jump) and upper limb (medicine ball throw) muscle power, lower limb (1RM squat and legpress) and upper limb (1RM bench press) strength and speed (linear and with change of direction) tests were performed pre and post the pre-competitive period and post the first phase of the competitive period. ANOVA for repeated measures plus Bonferroni post hoc were used to compare the variables between the three evaluation moments (α = 0.05). Performance of the lower limb muscle power, linear speed and lower and upper limbs strength (1RM legpress and bench press) were improved at the three moments (p < 0.001). Body fat percentage, upper limb muscle power, lower limb strength (1RM squat) (p <0.001) and speed with change of direction (p = 0.002) improved the performance from pre to post pre-competitive period moment and kept this performance in the post competitive period. Body mass presented no significant difference between the moments. We concluded that the athletes improved physical fitness variables, proving the effectiveness of the training. Still, it is highlighted the importance of the training planning for the team success. (AU)