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Genomics ; 112(2): 1872-1878, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31678592

RESUMO

Whole genome sequencing (WGS) is a widely available, inexpensive means of providing a wealth of information about an organism's diversity and evolution. However, WGS for many pathogenic bacteria remain limited because they are difficult, slow and/or dangerous to culture. To avoid culturing, metagenomic sequencing can be performed directly on samples, but the sequencing effort required to characterize low frequency organisms can be expensive. Recently developed methods for selective whole genome amplification (SWGA) can enrich target DNA to provide efficient sequencing. We amplified Coxiella burnetii (a bacterial select agent and human/livestock pathogen) from 3 three environmental samples that were overwhelmed with host DNA. The 68- to 147-fold enrichment of the bacterial sequences provided enough genome coverage for SNP analyses and phylogenetic placement. SWGA is a valuable tool for the study of difficult-to-culture organisms and has the potential to facilitate high-throughput population characterizations as well as targeted epidemiological or forensic investigations.


Assuntos
Coxiella burnetii/genética , Genoma Bacteriano , Metagenoma , Animais , Coxiella burnetii/classificação , Coxiella burnetii/isolamento & purificação , Feminino , Cabras/microbiologia , Metagenômica/métodos , Leite/microbiologia , Filogenia , Polimorfismo de Nucleotídeo Único , Sequenciamento Completo do Genoma/métodos
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