RESUMO
PURPOSE: To compare immediate results of mitral valve surgery in patients with severe pulmonary hypertension with versus without concomitant pulmonary artery denervation. MATERIALS AND METHODS: From January to December 2015 30 patients were randomly assigned into two groups. Patients of one group underwent mitral valve surgery (comparison group), in the other group mitral valve surgery was accompanied by pulmonary artery ablation (ablation group). Both groups had comparable anthropometric, echocardiographic, and hemodynamics characteristics. RESULTS: There were no hospital mortality and specific ablation-related complications. Left ventricular remodeling and decrease of pulmonary artery pressure were observed in both groups at discharge. On the third day after surgery systolic and mean pulmonary artery pressure assessed by right heart catheterization were 48 and 22 versus 59 and 39 mm Hg in ablation and comparison group, respectively (p.
Assuntos
Hipertensão Pulmonar , Valva Mitral , Cateterismo Cardíaco , Hemodinâmica , Humanos , Hipertensão Pulmonar/terapia , Artéria PulmonarRESUMO
AIM: To evaluate prospectively the hemodynamic performance of «BioLAB Mono¼ stentless bioprosthesis implanted into aortic position. MATERIAL AND METHODS: Twenty seven patients (mean age 71 (67; 73); 17 women) with severe aortic stenosis underwent aortic valve replacement with «BioLAB Mono¼ stentless bioprosthesis from 2012 to 2014. The valves were implanted into supra-annular position using continuous polypropylene suture. RESULTS: In the early postoperative period 1 patient (3.7%) died for acute heart failure. The mean aortic cross-clamping time was 81 (75; 90) min. Echocardiographic peak pressure gradient were 18 (16; 23) mmHg (postoperative). There were no cases of valve dysfunction in early postoperative period. Level of thrombocytes recovered after 10 days postoperatively. CONCLUSION: «BioLAB Mono¼ aortic bioprosthesis implantation is easy and reproducible. The valve has excellent hemodynamic performance in early postoperative period.
Assuntos
Estenose da Valva Aórtica/cirurgia , Bioprótese , Insuficiência Cardíaca , Implante de Prótese de Valva Cardíaca , Próteses Valvulares Cardíacas , Complicações Pós-Operatórias , Desenho de Prótese/métodos , Idoso , Valva Aórtica/cirurgia , Estenose da Valva Aórtica/diagnóstico , Estenose da Valva Aórtica/fisiopatologia , Ecocardiografia/métodos , Feminino , Insuficiência Cardíaca/diagnóstico , Insuficiência Cardíaca/etiologia , Insuficiência Cardíaca/fisiopatologia , Insuficiência Cardíaca/prevenção & controle , Implante de Prótese de Valva Cardíaca/efeitos adversos , Implante de Prótese de Valva Cardíaca/métodos , Hemodinâmica , Humanos , Masculino , Duração da Cirurgia , Complicações Pós-Operatórias/diagnóstico , Complicações Pós-Operatórias/fisiopatologia , Complicações Pós-Operatórias/prevenção & controle , Estudos Prospectivos , Reprodutibilidade dos Testes , Índice de Gravidade de Doença , Sibéria , Técnicas de Sutura , Resultado do TratamentoRESUMO
AIM: To evaluate prospectively the hemodynamic performance of «BiolAB Mono¼ stentless bioprosthesis implanted into aortic position. MATERIAL AND METHODS: Twenty seven patients (mean age 71 (67; 73); 17 women) with severe aortic stenosis underwent, aortic valve replacement with «BioLAB Mono¼ stentless bioprosthesis from 2012 to 2014. The valves- were implanted into supraannular position using continuous polypropylene suture. RESULTS: In the early postoperative period 1 patient (3.7%) died for acute heart failure. The mean aortic cross-clamping time was 81 (75; 90) min. Echocardiographic peak pressure gradient were 18 (16;23) mmHg (postoperative). There were no cases of valve dysfunction In early 'postoperative period. Level of thrombocytes recovered after 1 O days postoperatively. CONCLUSION: «BioLAB Mono¼ aortic bioprosthesis implantation. is easy and reproducible. The valve has excellent hemodynamic performance ir;i early postoperative period.
Assuntos
Estenose da Valva Aórtica , Valva Aórtica/cirurgia , Bioprótese , Insuficiência Cardíaca , Implante de Prótese de Valva Cardíaca , Próteses Valvulares Cardíacas , Complicações Pós-Operatórias , Idoso , Estenose da Valva Aórtica/diagnóstico , Estenose da Valva Aórtica/mortalidade , Estenose da Valva Aórtica/fisiopatologia , Estenose da Valva Aórtica/cirurgia , Ecocardiografia/métodos , Feminino , Insuficiência Cardíaca/diagnóstico , Insuficiência Cardíaca/etiologia , Insuficiência Cardíaca/fisiopatologia , Insuficiência Cardíaca/prevenção & controle , Implante de Prótese de Valva Cardíaca/efeitos adversos , Implante de Prótese de Valva Cardíaca/métodos , Hemodinâmica , Humanos , Tempo de Internação , Masculino , Duração da Cirurgia , Complicações Pós-Operatórias/diagnóstico , Complicações Pós-Operatórias/fisiopatologia , Complicações Pós-Operatórias/prevenção & controle , Estudos Prospectivos , Índice de Gravidade de Doença , Sibéria , Técnicas de Sutura , Resultado do TratamentoRESUMO
This study examines the efficacy and safety of the natural complex of multi-component biologically active dietary supplement Prostatinol in the pathogenetic treatment of 45 patients with chronic nonbacterial Prostatitis complicated by sperm disorders. Within 4 weeks 25 patients of the treatment group and 20 patients of the control group received Prostatinol or traditional anti-inflammatory therapy correspondingly. The results were evaluated at weeks 4 and 12 after treatment cessation. Prostatinol was found to significantly increase the treatment effectiveness. More pronounced positive clinical effect was noted in 92% of treatment group patients receiving Prostatinol (disappearance of perineal pain, reduction of the IPSS score to an average of 2.1 points, absence of residual urine, normal Prostate echotexture, improvement of Prostate circulation, increase of sperm concentration and motility, higher pregnancy incidence among the wives of the treated patients). In the control group similar treatment effect was achieved in twice less number of patients. These results confirm that the components of the Prostatinol complex are effective, safe, have no side effects and can be used in the treatment of chronic Prostatitis, as well as for prevention of Prostatic diseases and improvement of sexual function.
Assuntos
Infertilidade Masculina , Prostatite , Adulto , Doença Crônica , Feminino , Humanos , Infertilidade Masculina/complicações , Infertilidade Masculina/tratamento farmacológico , Infertilidade Masculina/fisiopatologia , Masculino , Pessoa de Meia-Idade , Gravidez , Prostatite/complicações , Prostatite/tratamento farmacológico , Prostatite/fisiopatologiaRESUMO
Based on the analysis of 20 different monocot and eudicot species, we propose that the centromeric distribution of the phosphorylated histone H2AThr120 is evolutionary highly conserved across species with mono- and holocentric chromosomes. Therefore, antibodies recognizing the phosphorylated threonine 120 of the histone H2A can serve as a universal marker for the cytological detection of centromeres of mono- and holokinetic plant species. In addition, super resolution microscopy of signals specific to the centromere-specific histone H3 variant CENH3 and to H2AThr120ph revealed that these histone variants are incorporated into different nucleosomes, which form distinct, partly intermingled chromatin domains. This specific arrangement of both histone variants suggests different centromeric functions during the cell cycle.
Assuntos
Centrômero/genética , Cromatina/genética , Cromossomos de Plantas/genética , Histonas/genética , Histonas/metabolismo , Fosforilação/genética , Nucleossomos/genéticaRESUMO
442 patients underwent the mitral valve replacement (MVR) with KemCor and PeriCor stented bioprostheses. The study results justified the preference of the "totally biological" PeriCor valve in patients with active infectious endocarditis. The longest follow-up period was 11 years, the mean follow-up was 51±34 months. The risk of the structural valve disfunction was shown to be inversely proportional to the age (p=0.03). 38% of the operated women younger the 45, developed the structural valve disfunction in 79±27 months after surgery. Thus, MVR with stented bioprostheses proved to be clinically efficient early after the operation. Predictability of the results of KemCor and PeriCor valve implantation allows the theoretically substantiated approach to the rehabilitation, especially, considering the adverse event rate in different patient groups at certain follow-up periods.
Assuntos
Bioprótese , Doenças das Valvas Cardíacas , Implante de Prótese de Valva Cardíaca , Próteses Valvulares Cardíacas , Valva Mitral/cirurgia , Complicações Pós-Operatórias , Adulto , Fatores Etários , Bioprótese/efeitos adversos , Bioprótese/normas , Feminino , Doenças das Valvas Cardíacas/classificação , Doenças das Valvas Cardíacas/mortalidade , Doenças das Valvas Cardíacas/cirurgia , Próteses Valvulares Cardíacas/efeitos adversos , Próteses Valvulares Cardíacas/normas , Implante de Prótese de Valva Cardíaca/efeitos adversos , Implante de Prótese de Valva Cardíaca/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Avaliação de Resultados em Cuidados de Saúde , Complicações Pós-Operatórias/classificação , Complicações Pós-Operatórias/etiologia , Complicações Pós-Operatórias/mortalidade , Fatores de Risco , Análise de Sobrevida , Resultado do TratamentoRESUMO
Effectiveness employment of reseach pectin containing drug for enterosoption cause content pectin substances (5-7%), and protein-carbonic complex-balanced and presence such a important immunecorrects, as arginin and glutamic asid, probiotic of metabiotic type establish nutritive support in enteric insufficiency syndrome treatment. There was marked a beneficial effect during using pectin containing drug in the postoperative treatment of intestinal insufficiency, as was confirmed by evaluation of biopsy specimens of gastric mucosa, jejunum and sigmoid colon. Morphologically, it was expressed in chronic inflammation without acute manifestations.
Assuntos
Enteropatias/tratamento farmacológico , Mucosa Intestinal/efeitos dos fármacos , Pectinas/química , Probióticos/química , Combinação de Medicamentos , Humanos , Enteropatias/etiologia , Enteropatias/patologia , Mucosa Intestinal/patologia , Pectinas/uso terapêutico , Peritonite/complicações , Probióticos/uso terapêutico , Síndrome , Tecnologia FarmacêuticaRESUMO
Outbred female SHR mice (n=200) were divided at random into 4 groups, 50 per group. Benz(a)pyrene solution in acetone was applied onto a skin site on the back during 26 weeks. In parallel, the animals received melatonin, metformin, or both. Melatonin, metformin, and their combination promoted significant reduction of the number and size of skin tumors. In mice receiving no therapy, benz(a)pyrene applications increased the concentrations of malonic dialdehyde in the serum and skin tumor tissue in comparison with the concentrations in the sera and skin of intact mice. Melatonin, metformin, and their combination normalized LPO level.
Assuntos
Transformação Celular Neoplásica/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos dos fármacos , Melatonina/farmacologia , Metformina/farmacologia , Neoplasias Cutâneas/induzido quimicamente , Neoplasias Cutâneas/prevenção & controle , Animais , Antioxidantes/farmacologia , Benzo(a)pireno , Transformação Celular Neoplásica/metabolismo , Feminino , Malondialdeído/sangue , Camundongos , Espécies Reativas de Oxigênio/metabolismo , Pele/patologia , Neoplasias Cutâneas/patologiaRESUMO
The spatial variations of photosynthetic picoplankton abundance and biomass and the picoplankton's contribution to chlorophyll a concentration along the transect from Khatanga Bay to the continental slope in the western part of the Laptev Sea were studied in September 2017. Picoeukaryotes dominated in the picophytoplankton communities. Picophytoplankton in Khatanga Bay showed more variability than those over the Laptev shelf and continental slope: abundance and biomass were the highest in the southern part of the bay and markedly decreased with increasing salinity in its northern part. Picocyanobacteria were found over the shelf and slope at temperatures of +2.4 to -1.6°Ð¡ and salinity from 22 to 34. Picophytoplankton contribution to total chlorophyll a on the shelf was higher than in Khatanga Bay. The study of picophytoplankton of Khatanga Bay and in the western Laptev Sea can serve as a baseline for future assessment of the Laptev Sea ecosystem response to interannual and climate changes.
RESUMO
There was marked a beneficial effect during using pectin containing drug in the postoperative treatment of intestinal insufficiency, as was confirmed by evaluation of biopsy specimens of gastric mucosa, jejunum and sigmoid colon. Morphologically, it was expressed in chronic inflammation without acute manifestations. Restoration of the morphological structure of the small intestinal mucosa at 7th day of the usual treatment was observed in 50% of patients but during treatment using a modified pectin containing drug at 80%, ie in 1,6 times more often.
Assuntos
Antidiarreicos/administração & dosagem , Enteropatias/patologia , Enteropatias/terapia , Mucosa Intestinal/patologia , Pectinas/administração & dosagem , Biópsia , Feminino , Humanos , MasculinoRESUMO
In this article was shown the efficiency of enterosorbtion in various departments of medicine. You can find a comparative assessment of different enterosorbents. Were presented results of own using of pectin containing sorbents for enterosorbtion and nutritional support in surgical treatment of endotoxemia on the background of intestinal insufficiency syndrome in 161 patients. Application of the PCD had reduced bed-day in 1,4 times and mortality--a 2.05-times in compare with conventional treatment in the control group of 171 patients of the paired samples.
Assuntos
Enteroadsorção/métodos , Enteropatias/terapia , Animais , Humanos , SíndromeRESUMO
The original conception of "surgecal endotoxicosis" is presentation in the artical. MATERIALS AND RESEARCH METHODS: 2064 of a patients and results of 320 autopsies was divided into 4 groups in depending with bowel dysfunctions. There is the new classification of surgecal endotoxicosis in this article. Also the authors propose some technologies of detoxication.
Assuntos
Endotoxemia/classificação , Endotoxemia/etiologia , Endotoxemia/patologia , Complicações Intraoperatórias/classificação , Complicações Intraoperatórias/patologia , Feminino , Humanos , Enteropatias/patologia , Enteropatias/cirurgia , MasculinoRESUMO
Surgical endotoxicosis entails dysfunction of all organs and systems including immune system that considerably aggravates severity of current disease and slows down a recovery in the postoperative period. Searching of various ways of immunocorrection in peritonitis of various etiology is the actual commitment of the contemporary surgery. The complex method of simultaneous enterosorbtion and immunocorrection with the modified pectin contained specimen in patients with a peritonitis is offered. In study the role natural enteral sorbents in optimization of the immune answer due to restoration of microbiocenosis sorbtion and immunocorrection modified with the modified pectin contained intestines is proved. Entero specimen provide decreasing of medium molecular weight substances' level to 89.0% in reactive stage of a peritonitis and to 69.0%--in toxic stage during 3 days. By 5th day of treatment the level of medium molecular weight substances and leucocyte intoxication index were already reached to the normal parameters. It allows us to recommend this introducted method in a complex of medical treatment in postoperation period in peritonitis.
Assuntos
Antidiarreicos/farmacologia , Nutrição Enteral , Enteroadsorção/métodos , Pectinas/farmacologia , Peritonite/imunologia , Peritonite/terapia , Animais , Humanos , Masculino , Peritonite/etiologia , Cuidados Pós-Operatórios , Ratos , Ratos Wistar , Fatores de TempoRESUMO
Experimental studies on 26 rats showed a stimulating effect of beet pectin at small intestine with paresis, mucosal repair, and bactericidal action on intestinal microflora. In comparison of activity intensity of small intestine solder at 1 day was 2.5-1.75 times less than 3 days and it is not reached. In the experimental group at 1 day solder activity was lower than the norm for the divisions in the 1.7-1.64--1.53-fold, and by 3 days, respectively, exceeded the norm of 0.8-2.4--2.25 times. We examined 150 patients aged 19 to 86 years with poured peritonitis primarily toxic (85.3%). III. The main causes of peritonitis were acute appendicitis (24.0%) and perforation of gastroduodenal ulcers (21.3%). For a comparative analysis of results of treatment were formed 3 patient groups using paired sample. Control group patients received conventional treatment. Enterosorption conducted through nazointestinalny probe known carbon sorbent UAO-A and pektincontents products (SAPs) the dining beet sublimate materials. The results are compared. Reducing the time used to resolve paresis of intestine and nazointestinal intubation in 1,3 times, reducing the severity of the patient on a scale of SAPS is already at 2 hours improving immunity more rapid decline in toxicity of blood plasma and leukocyte index of intoxication, reduction of postoperative pneumonia in 12% and postoperative mortality from 14% to 6.6% showed the advantages enterosorption modified pectin contents drug.
Assuntos
Enteroadsorção/métodos , Motilidade Gastrointestinal/efeitos dos fármacos , Pectinas/uso terapêutico , Peritonite/terapia , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Beta vulgaris/química , Motilidade Gastrointestinal/fisiologia , Humanos , Intestino Delgado/efeitos dos fármacos , Intestino Delgado/microbiologia , Intubação Gastrointestinal , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Pectinas/isolamento & purificação , Pectinas/farmacologia , Peritonite/microbiologia , Peritonite/fisiopatologia , Índice de Gravidade de Doença , Resultado do Tratamento , Adulto JovemRESUMO
Cell cycle dependent phosphorylation of conserved N-terminal tail residues of histone H3 has been described in both animal and plant cells. Through cytogenetic approaches using different plant species we show a detailed description of distribution patterns of phosphorylated histone H3 at either threonine 3 or threonine 32 in mitosis and meiosis. In meristematic cells of the large genome species Secale cereale, Vicia faba and Hordeum vulgare we have found that phosphorylation of both threonine residues begins in prophase, and dephosphorylation occurs in late anaphase. However, in the small genome species Arabidopsis thaliana dephosphorylation occurs at anaphase. In the first division of meiosis of species with large genomes phosphorylation of histone H3 at either threonine 3 or threonine 32 is seen first in diakinesis and extends to anaphase I, whereas in the second division these post-translational modifications are visible at metaphase II through anaphase II. While in A. thaliana dephosphorylation takes place at anaphase I and II. In all species analysed phosphorylated H3 at either threonine 3 or threonine 32 are distributed along the entire length of chromosomes during mitotic metaphase and metaphase I. In the second meiotic division threonine 3 phosphorylation is restricted to the pericentromeric domain, while phosphorylation of threonine 32 is widespread along chromosome arms of all species analysed.
Assuntos
Histonas/química , Histonas/metabolismo , Células Vegetais , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Plantas/metabolismo , Arabidopsis/genética , Arabidopsis/metabolismo , Ciclo Celular , Cromossomos de Plantas/genética , Cromossomos de Plantas/metabolismo , Histonas/genética , Hordeum/genética , Hordeum/metabolismo , Hibridização in Situ Fluorescente , Meiose , Mitose , Fosforilação , Proteínas de Plantas/genética , Plantas/genética , Processamento de Proteína Pós-Traducional , Secale/genética , Secale/metabolismo , Especificidade da Espécie , Treonina/química , Vicia faba/genética , Vicia faba/metabolismoRESUMO
The efficacy of multimodality conservative treatment for prostate and bladder cancer and cervical carcinoma was improved due to integration of such modem modalities as intra-arterial chemotherapy, local hyperthermia and hyperglycemia and combination of local and systemic radiomodifiers. Our methods use criteria of actual survival and are intended to raise it.
Assuntos
Pesquisa Biomédica , Radioterapia , Academias e Institutos , Animais , Feminino , Órgãos Governamentais , Humanos , Terapia com Luz de Baixa Intensidade , Masculino , Neoplasias da Próstata/radioterapia , Radiossensibilizantes/uso terapêutico , Radioterapia/métodos , Radioterapia/tendências , Neoplasias da Glândula Tireoide/radioterapia , Neoplasias da Bexiga Urinária/radioterapia , Neoplasias do Colo do Útero/radioterapiaRESUMO
A novel mitosis-specific phosphorylation site in histone H3 at threonine 11 has been described for mammalian cells. This modification is restricted to the centromeric region while phosphorylation at the classical H3 sites, Ser10 and Ser28 occurs along the entire chromosomal arms. Using phosphorylation state-specific antibodies we found that phosphorylation at threonine 11 occurs also in plant cells, during mitosis as well as meiosis. However, in contrast to animal cells, ph(Thr11)H3 was distributed along the entire length of condensed chromosomes, whereas H3 phosphorylated at Ser10 and Ser28 appeared to be restricted to centromeric/pericentromeric chromatin. Phosphorylation at Thr11 started in prophase and ended in telophase, it correlated with the condensation of mitotic and meiotic chromosomes and was independent of the distribution of late replicating heterochromatin and Giemsa-banding positive regions. Interestingly, treatment of cells with the phosphatase inhibitor cantharidin revealed a high level of Thr11 phosphorylation in interphase cells, in this case particularly in pericentromeric regions. These data show that histone modifications are highly dynamic. Moreover, animal and plant organisms may have evolved individual histone codes.
Assuntos
Arabidopsis/metabolismo , Histonas/metabolismo , Hordeum/metabolismo , Fosfotreonina/metabolismo , Secale/metabolismo , Treonina , Vicia faba/metabolismo , Arabidopsis/genética , Cromossomos de Plantas/genética , Cromossomos de Plantas/metabolismo , Heterocromatina/metabolismo , Heterocromatina/ultraestrutura , Hordeum/genética , Imuno-Histoquímica , Meiose , Microscopia Eletrônica , Mitose , Fosforilação , Fosfosserina/metabolismo , Proteínas de Plantas/metabolismo , Secale/genética , Vicia faba/genéticaRESUMO
UNLABELLED: One hundred and fifty patients aged 19 to 86 years with general peritonitis mainly in a toxic (85.3%) stage were examined. The leading causes of peritonitis were acute appendicitis (24.0%) and perforation of gastroduodenal ulcers (21.3%). Three groups of patients were formed with a pair samples method for a comparative analysis of treatment RESULTS: The control group of patients was treated conventionally. Entero-sorption was performed through naso-intestinal tube with well-known carbonic sorbent UAO-A and pectin-containing medication made of red beet. The results were compared. A 1.3-fold decrease in entero-paresis naso-intestinal intubation duration, a relief in severity of the patients' condition by SAPS scale as early as on day 2, improvement of immunity indices, faster reduction of toxicity of blood plasma and leukocytic index of intoxication, a 12% decrease in postoperative pneumonia morbidity and postoperative lethality from 14 to 6.6% demonstrated advantages of entero-sorption with pectin-containing medication.
Assuntos
Enteroadsorção , Pectinas/administração & dosagem , Peritonite/cirurgia , Peritonite/terapia , Doença Aguda , Adulto , Idoso , Idoso de 80 Anos ou mais , Apendicite/complicações , Enteroadsorção/métodos , Seguimentos , Humanos , Pessoa de Meia-Idade , Úlcera Péptica Perfurada/complicações , Peritonite/diagnóstico , Peritonite/etiologia , Peritonite/imunologia , Complicações Pós-Operatórias , Fatores de TempoRESUMO
Fifty-eight patients aged from 18 to 65 years with peritonitis of different etiology in toxic and terminal phases were treated. Nasoenteral intubation, enterosorption and early nutritious support with pectin-containing preparation (PCP) were carried out in all the patients just after surgery with standard course of 5 days. Baseline data was compared with that after PCP-supported treatment. Powder products of red beet were used as PCP. Immunodeficiency in peritonitis is characterized by imbalance of stress-realizing and stress-limiting mechanisms of immunocompetent cells. Enterosorption and early nutritious support with PCP in peritonitis decrease the level of plasmic cortisol and enhance stress-limiting reception that reduces a harmful effect of cortisol and reestablishes immunity.
Assuntos
Antidiarreicos/uso terapêutico , Enteroadsorção , Síndromes de Imunodeficiência/terapia , Apoio Nutricional , Pectinas/uso terapêutico , Peritonite/complicações , Estresse Fisiológico/imunologia , Adolescente , Adulto , Idoso , Linfócitos B/imunologia , Relação CD4-CD8 , Seguimentos , Humanos , Imunidade Celular/imunologia , Síndromes de Imunodeficiência/etiologia , Síndromes de Imunodeficiência/imunologia , Intubação Gastrointestinal , Pessoa de Meia-Idade , Peritonite/imunologia , Estresse Fisiológico/complicações , Linfócitos T/imunologia , Resultado do TratamentoRESUMO
Immunolabeling using site-specific antibodies against phosphorylated histone H3 at serine 10 or serine 28 revealed in plants an almost similar temporal and spatial pattern of both post-translational modification sites at mitosis and meiosis. During the first meiotic division the entire chromosomes are highly H3 phosphorylated. In the second meiotic division, like in mitosis, the chromosomes contain high phosphorylation levels in the pericentromeric region and very little H3 phosphorylation along the arms of monocentric species. In the polycentric plant Luzula luzuloides phosphorylation at both serine positions occurs along the whole chromosomes, whereas in monocentric species, only the pericentromeric regions showed strong signals from mitotic prophase to telophase. No phosphorylated serine 10 or serine 28 was detectable on single chromatids at anaphase II resulting from equational segregation of rye B chromosome univalents during the preceding anaphase I. In addition, we found a high level of serine 28 as well as of serine 10 phosphorylation along the entire mitotic monocentric chromosomes after treatment of mitotic cells using the phosphatase inhibitor cantharidin. These observations suggest that histone H3 phosphorylation at serine 10 and 28 is an evolutionarily conserved event and both sites are likely to be involved in the same process, such as sister chromatid cohesion.