Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Mais filtros

Base de dados
País/Região como assunto
Tipo de documento
Intervalo de ano de publicação
1.
Plants (Basel) ; 12(10)2023 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-37653936

RESUMO

Rice is an important staple food crop for over half of the world's population. However, abiotic stresses seriously threaten rice yield improvement and sustainable production. Breeding and planting rice varieties with high environmental stress tolerance are the most cost-effective, safe, healthy, and environmentally friendly strategies. In-depth research on the molecular mechanism of rice plants in response to different stresses can provide an important theoretical basis for breeding rice varieties with higher stress resistance. This review presents the molecular mechanisms and the effects of various abiotic stresses on rice growth and development and explains the signal perception mode and transduction pathways. Meanwhile, the regulatory mechanisms of critical transcription factors in regulating gene expression and important downstream factors in coordinating stress tolerance are outlined. Finally, the utilization of omics approaches to retrieve hub genes and an outlook on future research are prospected, focusing on the regulatory mechanisms of multi-signaling network modules and sustainable rice production.

2.
Rice (N Y) ; 16(1): 51, 2023 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-37971600

RESUMO

Root hairs are extensions of epidermal cells on the root tips that increase the root contract surface area with the soil. For polar tip growth, newly synthesized proteins and other materials must be incorporated into the tips of root hairs. Here, we report the characterization of PRX102, a root hair preferential endoplasmic reticulum peroxidase. During root hair growth, PRX102 has a polar localization pattern within the tip regions of root hairs but it loses this polarity after growth termination. Moreover, PRX102 participates in root hair outgrowth by regulating dense cytoplasmic streaming toward the tip. This role is distinct from those of other peroxidases playing roles in the root hairs and regulating reactive oxygen species homeostasis. RNA-seq analysis using prx102 root hairs revealed that 87 genes including glutathione S-transferase were downregulated. Our results therefore suggest a new function of peroxidase as a player in the delivery of substances to the tips of growing root hairs.

3.
Nat Prod Res ; 36(9): 2440-2445, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-33179516

RESUMO

Scutellaria araxensis, S. bornmuelleri and S. orientalis are traditional herbs widely used by native Iranians for the treatment various ailments. In the current study, we used HPLC-DAD-ESI/MSn to investigate the presence of phenolic compounds of root and shoot methanolic extracts. The flavonoid composition were identified based on mass of pseudomolecular [M - H]- ions and fragmentation spectra and comparisons with literature data. A total of 26, 25, 17, 23, 19 and 26 flavonoids were identified from roots and shoots of S. araxensis, S. bornmuelleri and S. orientalis, respectively. The obtained phenolic profiles revealed the presence of the major flavonoid components of Scutellaria genus including baicalein, wogonin, scutellarein and their glycosides, as well as chrysin, tricin, skullcapflavon II, pinocembrin and phenylethanoid glycosides such as acteoside and verbascoside. This study, in addition to reveal the potency of chromatographic profiling for identification of Scutellaria flavonoids, introduces this species as a potential source of bioactive metabolites.


Assuntos
Scutellaria , Cromatografia Líquida de Alta Pressão/métodos , Flavonoides/análise , Glicosídeos/análise , Humanos , Irã (Geográfico) , Extratos Vegetais/química , Scutellaria/química
4.
Plants (Basel) ; 9(5)2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32392904

RESUMO

Crop cultivars with larger root systems have an increased ability to absorb water and nutrients under conditions of water deficit. To unravel the molecular mechanism of water-stress tolerance in wheat, we performed RNA-seq analysis on the two genotypes, Colotana 296-52 (Colotana) and Tincurrin, contrasting the root growth under polyethylene-glycol-induced water-stress treatment. Out of a total of 35,047 differentially expressed genes, 3692 were specifically upregulated in drought-tolerant Colotana under water stress. Transcription factors, pyrroline-5-carboxylate reductase and late-embryogenesis-abundant proteins were among upregulated genes in Colotana. Variant calling between Colotana and Tincurrin detected 15,207 SNPs and Indels, which may affect protein function and mediate the contrasting root length phenotype. Finally, the expression patterns of five triads in response to water, high-salinity, heat, and cold stresses were analyzed using qRT-PCR to see if there were differences in homoeologous gene expression in response to those conditions. The five examined triads showed variation in the contribution of homoeologous genes to water, high-salinity, heat, and cold stresses in the two genotypes. The variation of homoeologous gene expression in response to environmental stresses may enable plants to better cope with stresses in their natural environments.

5.
PLoS One ; 15(4): e0230820, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32298285

RESUMO

The high toxicity of cadmium (Cd) and its ready uptake by plants has become a major agricultural problem. To investigate the genetic architecture and genetic regulation of Cd tolerance in barley, we conducted quantitative trait loci (QTL) analysis in the phenotypically polymorphic Oregon Wolfe Barley (OWB) mapping population, derived from a cross between Rec and Dom parental genotypes. Through evaluating the Cd tolerance of 87 available doubled haploid lines of the OWB mapping population at the seedling stage, one minor and one major QTL were detected on chromosomes 2H and 6H, respectively. For chlorosis and necrosis traits, the major QTL explained 47.24% and 38.59% of the phenotypic variance, respectively. RNA-Seq analysis of the parental seedlings under Cd treatment revealed 542 differentially expressed genes between Cd-tolerant Rec and Cd-susceptible Dom genotypes. By analyzing sequence variations in transcribed sequences of the parental genotypes, 155,654 SNPs and 1,525 InDels were identified between the two contrasting genotypes and may contribute to Cd tolerance. Finally, by integrating the data from the identified QTLs and RNA-Seq analysis, 16 Cd tolerance-related candidate genes were detected, nine of which were metal ion transporters. These results provide promising candidate genes for further gene cloning and improving Cd tolerance in barley.


Assuntos
Cádmio/toxicidade , Tolerância a Medicamentos/genética , Hordeum/efeitos dos fármacos , Hordeum/genética , Locos de Características Quantitativas/genética , Mapeamento Cromossômico/métodos , Cromossomos de Plantas/efeitos dos fármacos , Cromossomos de Plantas/genética , Haploidia , Oregon , Fenótipo , Polimorfismo de Nucleotídeo Único/genética , RNA-Seq/métodos , Plântula/efeitos dos fármacos , Plântula/genética , Estresse Fisiológico/genética , Sequenciamento do Exoma/métodos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA