RESUMO
A new class of acrylamides was synthesized, and the effects of these analogues on outward potassium current were evaluated by using two electrode voltage clamp recordings from Xenopus laevis oocytes expressing cloned mKCNQ2 channels. SAR studies indicated that the pharmacophore of the acrylamide series includes the (S) absolute configuration at the (1-phenyl)ethyl moiety and the alpha,beta-unsaturated acrylamide functionality with a free NH. This study identified (S)-N-[1-(3-morpholin-4-yl-phenyl)-ethyl]-3-phenyl-acrylamide ((S)-1) and (S)-N-[1-(4-fluoro-3-morpholin-4-yl-phenyl)-ethyl]-3-(4-fluoro-phenyl)-acrylamide ((S)-2) as KCNQ2 openers for further electrophysiological evaluations. These two acrylamides demonstrated significant activity in the cortical spreading depression model of migraine as we reported previously.
Assuntos
Acrilamidas/síntese química , Cinamatos/síntese química , Morfolinas/síntese química , Canais de Potássio/efeitos dos fármacos , Acrilamidas/química , Acrilamidas/farmacologia , Animais , Cinamatos/química , Cinamatos/farmacologia , Depressão Alastrante da Atividade Elétrica Cortical/efeitos dos fármacos , Humanos , Canal de Potássio KCNQ2 , Camundongos , Morfolinas/química , Morfolinas/farmacologia , Oócitos/efeitos dos fármacos , Oócitos/fisiologia , Técnicas de Patch-Clamp , Canais de Potássio/fisiologia , Canais de Potássio de Abertura Dependente da Tensão da Membrana , Estereoisomerismo , Relação Estrutura-Atividade , Xenopus laevisRESUMO
A series of indole tetrahydropyridine and indole cyclohexenylamines was prepared, and their binding affinities at the human serotonin transporter (SERT) were determined. In particular, a nitrile substituent at the C5 position of the indole ring gave potent SERT activity. The stereochemistry of the N,N-dimethylamine substituent was determined for the most potent indole cyclohexenylamine, 6a. The enantiomers of 6a were energy minimized and compared to other conformationally restricted SSRIs. Compound 6a was found to give a dose-response similar to the SSRI fluoxetine in microdialysis studies in rats.
Assuntos
Inibidores Seletivos de Recaptação de Serotonina/química , Inibidores Seletivos de Recaptação de Serotonina/metabolismo , Proteínas da Membrana Plasmática de Transporte de Serotonina/metabolismo , Triptaminas/química , Animais , Cicloexenos/síntese química , Cicloexenos/química , Cicloexenos/farmacologia , Fluoxetina/química , Fluoxetina/farmacologia , Humanos , Indóis/síntese química , Indóis/química , Indóis/farmacologia , Microdiálise , Conformação Molecular , Piridinas/síntese química , Piridinas/química , Piridinas/farmacologia , Ratos , Inibidores Seletivos de Recaptação de Serotonina/síntese químicaRESUMO
Novel C-5 substituted pyrrolotriazines were optimized for dual EGFR and HER2 protein tyrosine kinase inhibition. The lead compound exhibited promising oral efficacy in both EGFR and HER2 driven human tumor xenograft models. It is hypothesized that its C-5 morpholine side chain binds in the ribose phosphate portion of the ATP binding pocket.
Assuntos
Química Farmacêutica/métodos , Receptores ErbB/antagonistas & inibidores , Pirróis/síntese química , Receptor ErbB-2/antagonistas & inibidores , Triazinas/síntese química , Trifosfato de Adenosina/química , Animais , Células CACO-2 , Desenho de Fármacos , Inibidores Enzimáticos/farmacologia , Humanos , Concentração Inibidora 50 , Camundongos , Transplante de Neoplasias , Fosfatos/química , Pirróis/farmacologia , Ribose/química , Triazinas/farmacologiaRESUMO
Bioisosteric replacement studies led to the identification of N-(1-benzo[1,3]dioxol-5-yl-ethyl)-3-(2-chloro-phenyl)-acrylamide ((S)-3) as a highly potent KCNQ2 opener, and 3-(2,6-difluoro-phenyl)-N-[1-(2,3-dihydro-benzofuran-5-yl)-ethyl]-acrylamide ((S)-4), and N-[1-(2,3-dihydro-1H-indol-5-yl)-ethyl]-3-(2-fluoro-phenyl)-acrylamide ((S)-5) as highly efficacious KCNQ2 openers. In contrast, their respective R enantiomers showed significantly less or no appreciable KCNQ2 opener activity even at the highest concentration tested (10 microM). Because of its high potency and moderate efficacy as well as its convenient synthesis, (+/-)-3 was selected as a reference compound for analyzing efficacies of KCNQ openers in electrophysiology studies. Compounds (S)-4 and (S)-5 demonstrated significant activity in reducing neuronal hyperexcitability in rat hippocampal slices. The synthesis and the KCNQ2 opener activity of these acrylamides are described.