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Brucellosis is a highly contagious zoonotic disease and a major human health problem worldwide. Due to its ways of transmission, direct or indirect contact with infected animals or their contaminated biological products, the disease exhibits strong occupational association with animal handlers comprising a significant population at risk. This study was undertaken to estimate the seroprevalence of brucellosis in animal handlers and to understand the epidemiological and serological aspects of the same. The animal handlers from the state of West Bengal, India were included in this study. It was a prospective and observational cohort study from November 2021 to March 2022. A total of 669 sera samples were collected from animal handlers and tested using various serological tests for Brucella antibodies. All serum samples were tested using the Rose Bengal plate test (RBPT), standard tube agglutination test (STAT), and enzyme-linked immunosorbent assay (ELISA). 106 (15.8%) patients were diagnosed with brucellosis among the total number of patients tested. Most of the patients affected with brucellosis belonged to the age group 51-60 years (23.5%). The seropositivity rate in male animal handlers was higher than female animal handlers in this study. More studies are needed to understand the occupational association of this disease. Awareness programs, safe livestock practices, and prevention of the disease by timely diagnosis must be implemented in order to control human brucellosis.
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Introduction: “Hospital-acquired infection (HAI)” can be defined as an infection acquired in hospital by a patient who wasadmitted for a reason other than that infection.Purpose: This study was done to observe the incidence of HAI (with bacteriological profile and their antibiotic susceptibilitypattern) in the Orthopaedics Ward of Bankura Sammilani Medical College.Materials and Methods: A total of 2062 patients admitted for more than 48 h were studied for 1 year for the detection of thedevelopment of any of the HAI during hospitalization. On suspicion of the development of an HAI, representative samples forthe diagnosis of a site-specific HAI were collected, processed, and interpreted. A total of 158 patients admitted for more than48 h showing clinical features of any one of the HAIs were selected for sampling.Results: From the 158 samples collected during the study period, samples from 92 patients were positive in bacteriological culture.Conclusion: The study attempts to understand the epidemiological profile of HAI in this tertiary care hospital in a non-urbanregion of eastern India to take necessary infection control measures.
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Biofilms have been classically visualized by Scanning Electron Microscopy (SEM). The complex operating procedure of SEM restricts its use in routine practice. There is a need of newer visualizing techniques for examining surfaces of biofilms, in particular under ambient conditions. We have presented the unique advantages of atomic force microscopy (AFM) in studying surfaces of biofilms through analyses of the height images obtained on biofilms of two gram positive and one gram negative bacteria, namely Staphylococcus aureus, Nocardia brasiliensis and Pseudomonas aeruginosa, respectively. Biofilm quality of the three different bacteria, ageing effects on Nocardia spp. biofilm surface and effects of the antibiotic ciprofloxacin at different doses on Staphylococcus and Pseudomonas biofilm surfaces have been investigated under ambient conditions and distinctive features have been observed.
Assuntos
Biofilmes/efeitos dos fármacos , Microscopia de Força Atômica/métodos , Nocardia/crescimento & desenvolvimento , Pseudomonas aeruginosa/crescimento & desenvolvimento , Staphylococcus aureus/crescimento & desenvolvimento , Antibacterianos/farmacologia , Aderência Bacteriana , Ciprofloxacina/farmacologia , Microscopia Eletrônica de Varredura , Nocardia/citologia , Nocardia/efeitos dos fármacos , Cimento de Policarboxilato , Pseudomonas aeruginosa/citologia , Pseudomonas aeruginosa/efeitos dos fármacos , Staphylococcus aureus/citologia , Staphylococcus aureus/efeitos dos fármacos , Propriedades de SuperfícieRESUMO
We report a case of 35-year-old male patient who presented with painful right side of scrotum and worm pouting out of the scrotum. The patient had undergone surgery for strangulated right inguinal hernia 2 years back. On exploration we found multiple adult Ascaris worms in the scrotum with right-sided hydrocele. All the worms were removed and eversion of sac was done.