The effects of storage temperature on PBMC gene expression.

BACKGROUND: Cryopreservation of peripheral blood mononuclear cells (PBMCs) is a common and essential practice in conducting research. There are different reports in the literature as to whether cryopreserved PBMCs need to only be stored ≤ -150 °C or can be stored for a specified time at -80 °C. Therefore, we performed gene expression analysis on cryopreserved PBMCs stored at both temperatures for 14 months and PBMCs that underwent temperature cycling 104 times between these 2 storage temperatures. Real-time RT-PCR was performed to confirm the involvement of specific genes associated with identified cellular pathways. All cryopreserved/stored samples were compared to freshly isolated PBMCs and between storage conditions. RESULTS: We identified a total of 1,367 genes whose expression after 14 months of storage was affected >3 fold in PBMCs following isolation, cryopreservation and thawing as compared to freshly isolated PBMC aliquots that did not undergo cryopreservation. Sixty-six of these genes were shared among two or more major stress-related cellular pathways (stress responses, immune activation and cell death). Thirteen genes involved in these pathways were tested by real-time RT-PCR and the results agreed with the corresponding microarray data. There was no significant change on the gene expression if the PBMCs experienced brief but repetitive temperature cycling as compared to those that were constantly kept ≤ -150 °C. However, there were 18 genes identified to be different when PBMCs were stored at -80 °C but did not change when stored < -150 °C. A correlation was also found between the expressions of 2'-5'- oligoadenylate synthetase (OAS2), a known interferon stimulated gene (IFSG), and poor PBMC recovery post-thaw. PBMC recovery and viability were better when the cells were stored ≤ -150 °C as compared to -80 °C. CONCLUSIONS: Not only is the viability and recovery of PBMCs affected during cryopreservation but also their gene expression pattern, as compared to freshly isolated PBMCs. Different storage temperature of PBMCs can activate or suppress different genes, but the cycling between -80 °C and -150 °C did not produce significant alterations in gene expression when compared to PBMCs stored ≤ -150 °C. Further analysis by gene expression of various PBMC processing and cryopreservation procedures is currently underway, as is identifying possible molecular mechanisms.

Body Mass Index, Adherence to a Healthy Lifestyle, and Breakfast Consumption Associated with Religious Affiliation in Peruvian University Students: A Cross-Sectional Study.

BACKGROUND: Despite evidence suggesting a relationship between religiosity and health habits, there is a paucity of studies specifically examining this association in the context of Peruvian university students. This study compared body mass index (BMI), adherence to a healthy lifestyle, and breakfast consumption in Peruvian university students of four religious denominations: Seventh Day Adventists (SDA), Catholics, Baptists, and Evangelicals. METHODS: A cross-sectional study was conducted online among 4557 students from a Peruvian university. The BMI and the frequency of breakfast consumption were evaluated, and the Diet and Healthy Lifestyle Scale (DEVS) was applied. The variables studied were associated using simple and multiple linear regression and Poisson models with robust variance. RESULTS: Baptist (B = 0.44, 95% CI: 0.10-0.78; p = 0.011), Catholic (B = 0.3, 95% CI: 0.12-0.47; p = 0.001), and Evangelical (B = 0.32, 95% CI: 0.09 to 0.64; p = 0.014) students had a significantly higher BMI compared to SDA. Baptist (B = -0.2, 95% CI: -0.37--0.05; p = 0.017) and Evangelical (B = -0.13, 95% CI: -0.28--0.03; p = 0.012) students exhibited a lower mean score on the measure of healthy lifestyles compared to SDA students. Additionally, Baptist (PR = -0.32, 95% CI: -0.92--0.12; p = 0.035) and Catholic (PR = -0.3, 95% CI: -0.99--0.19; p = 0.016) students exhibited a lower probability of eating breakfast regularly compared to SDA students. CONCLUSIONS: Health professionals should consider these findings when designing and implementing health promotion programs that are culturally sensitive and respectful of the beliefs and practices of all religious groups in university settings.

Description of a new genus and species of Eucoilinae (Hymenoptera: Cynipoidea: Figitidae) from Colombia.

Fontaliella, a new genus of Eucoilinae (Hymenoptera, Cynipoidea, Figitidae) and E. colombiensis sp. nov. from Colombia, are described and illustrated. This new genus belongs to the Ganaspini and morphologically resembles Zamischus Ashmead, 1903, Perischus Weld, 1931 and Steleucoela Kieffer, 1908. A key to differentiate these genera and species is given.

A model of metformin mitochondrial metabolism in metachromatic leukodystrophy: first description of human Schwann cells transfected with CRISPR-Cas9.

Metachromatic leukodystrophy is a neurological lysosomal deposit disease that affects public health despite its low incidence in the population. Currently, few reports are available on pathophysiological events related to enzyme deficiencies and subsequent sulfatide accumulation. This research aims to examine the use of metformin as an alternative treatment to counteract these effects. This was evaluated in human Schwann cells (HSCs) transfected or non-transfected with CRISPR-Cas9, and later treated with sulfatides and metformin. This resulted in transfected HSCs showing a significant increase in cell reactive oxygen species (ROS) production when exposed to 100 µM sulfatides (p = 0.0007), compared to non-transfected HSCs. Sulfatides at concentrations of 10 to 100 µM affected mitochondrial bioenergetics in transfected HSCs. Moreover, these analyses showed that transfected cells showed a decrease in basal and maximal respiration rates after exposure to 100 µM sulfatide. However, maximal and normal mitochondrial respiratory capacity decreased in cells treated with both sulfatide and metformin. This study has provided valuable insights into bioenergetic and mitochondrial effects of sulfatides in HSCs for the first time. Treatment with metformin (500 µM) restored the metabolic activity of these cells and decreased ROS production.

Cost-effective Evaluation of an Educational Intervention based on Multiple Intelligences Versus Traditional Care to Improve Exclusive Breastfeeding in Mothers' Clubs in Peru.

OBJECTIVES: This work sought to evaluate the cost-effectiveness of two educational interventions to improve exclusive maternal breastfeeding in mothers' clubs in Peru. METHODS: This is a retrospective, longitudinal descriptive study, which reviewed 113 information registries of mothers participating in a traditional educational intervention and 104 mothers with intervention based on multiple intelligences, evaluating the level of knowledge and exclusive maternal breastfeeding practices through observation visits. RESULTS: The intervention based on multiple intelligences achieved greater cost-effectiveness than the traditional intervention given that with lower investment, it managed to get 56.73% of the mothers to increase their knowledge and 68.27% to practice exclusive maternal breastfeeding from six months to more months in comparison with the traditional intervention in which 41.59% and 43.36% improved, respectively. Moreover, for its effective application, on every 100 mothers, its investment would result lower than the traditional program. CONCLUSIONS: Upon applying satisfactorily the cost-effectiveness evaluation, as model to compare educational interventions on exclusive maternal breastfeeding, better results were determined with the intervention based on multiple intelligences compared with the traditional intervention, given that with lower investment, it was possible to benefit a greater number of mothers in their level of knowledge and practices of maternal breastfeeding.

Seroprevalence of SARS-CoV-2 Infection among Occupational Groups from the Bucaramanga Metropolitan Area, Colombia.

The negative effects of coronavirus disease 2019 (COVID-19) pandemic have impacted the world economy due to the absence from work because of SARS-CoV-2 infection in workers, among other reasons. However, some economic areas are essential to society and people must continue working outside the home to support economic reactivation; their serological profile could be different from that of the global population. Cross-sectional study: Workers from health, construction, public transportation, public force, bike delivery messengers, independent or informal commerce areas, and residents of Bucaramanga or its metropolitan area were invited to participate. All participants self-completed a virtual survey and a blood test was taken to assess IgG and IgM with the ARC COV2 test. Seroprevalence was estimated considering a complex survey design, correcting for a finite population effect and adjusting for test performance. A total of 7045 workers were enrolled; 59.9% were women and most were residents of Bucaramanga and working in health occupations. The global adjusted seroprevalence was 19.5% (CI: 95% 18.6-20.4), being higher for Girón (27.9%; 95% CI: 24.5-31.30). Workers with multiple contact with people during working hours or using public transportation to go to work had a higher frequency of seropositivity for SARS-CoV-2. The seroprevalence among workers living in these four municipalities from the Colombian northeast area is still low.

Perception of COVID-19 Vaccination Amongst Physicians in Colombia.

Introduction: The SARS-CoV-2/COVID-19 pandemic has triggered the need to develop rapidly effective and safe vaccines to prevent infection, particularly in those at-risk populations such as medical personnel. This study's objective was to assess the perception of COVID-19 vaccination amongst Colombian physicians featuring two different scenarios of COVID-19 vaccination. Methods: A cross-sectional analytical study was carried out through an online survey directed at medical staff in several cities in Colombia. The percentage of physicians who have a positive perception to be vaccinated and the associated factors that determine that decision were determined. A binomial regression analysis adjusted for age and sex was carried out, taking as a dependent variable the acceptance of free vaccination with an effectiveness of 60 and 80%. The most significant factors were determined in the non-acceptance of vaccination. Results: Between 77.0% and 90.7% of physicians in Colombia accept COVID-19 vaccination, according to the scenario evaluated where the vaccine's effectiveness was 60 or 80%, respectively. Medical specialty, having never paid for a vaccine, recommending the administration of the vaccine to their parents or people over 70 years, and dispensing the vaccine to their children, were the factors to consider to be vaccinated for free with an effectiveness of 60% and 80%. Conclusions: There is a high perception of the intention to vaccinate physicians in Colombia against COVID-19, and this is very similar to that of the general population.

Pentraxin-3 is a candidate biomarker on the spectrum of severity from pre-eclampsia to HELLP syndrome: GenPE study.

Pentraxin-3 has been reported as a promising biomarker of pre-eclampsia and its severity; however, available studies have small sample sizes, and analyses are not always adjusted for confounders. The aim of this study is to establish the strength of the association between maternal Pentraxin-3 level and pre-eclampsia or HELLP syndrome. It was a case-control study. Women with pre-eclampsia or HELLP syndrome were defined as cases, and women with healthy pregnancies at term (>37 weeks) were classified as controls. Plasma concentrations of Pentraxin-3 were determined at the time of delivery by quantitative enzyme immunoassay. Associations between Pentraxin-3 and pre-eclampsia and HELLP syndrome were assessed by multinomial logistic regression. Subsidiary analysis for the time of disease onset was also carried out. Odds ratios and 95% confidence intervals are reported. A total of 1024 pregnant women were included (461 controls, 368 pre-eclampsia, 195 HELLP). A positive log-linear relationship was found between the top pentraxin-3 quintile and HELLP syndrome. After adjustment for confounders (maternal age, ethnicity, socioeconomic position, date and place of recruitment, family history of pre-eclampsia, smoking, body mass index at beginning of pregnancy, gestational age and multiple pregnancy), the strength of the association was higher for HELLP syndrome [OR 1.13 (95% CI 1.08; 1.18)] than for pre-eclampsia [OR 1.03 (95% CI 1.03; 1.10)]. No difference according to time of onset or pentraxin-3 level was found. In summary, pentraxin-3 level was associated with pre-eclampsia, but it was more strongly associated with HELLP syndrome. Longitudinal studies with a lower probability of residual confounding are necessary to improve our knowledge about the role of pentraxin-3 in pre-eclampsia.

[Immune response and gene therapy with adenoassociated viral vectors]. / Respuesta inmune y terapia génica con vectores virales adenoasociados.

In recent years, gene therapy has been positioned as a real and safe option in the development of therapeutic alternatives for the cure and prevention of different diseases. It consists in the insertion of genetic material in a defective tissue or cell, through the use of a vector. There are several considerations for selecting the most appropriate vector, including the potential for binding and entry to the target cell, the ability of the genetic material to transfer to the nucleus, the ability to express the insert, and the absence of toxicity. In the current scenario, the most commonly used viral vectors are those derived from adeno-associated viruses (AAV). Characteristics such as biosafety, low toxicity and selective tropism have enabled its evaluation as a therapeutic option in many monogenic or complex diseases. Despite their advantages, AAV vectors have drawbacks, the most important being the patient's immune response to the vector, especially the response mediated by neutralizing antibodies (NAb). NAbs decrease the transduction of the vector and prevent the expression of the gene it transports, limiting its clinical application. Therefore, identifying and quantifying the presence and activity of NAbs is the first step in any gene therapy protocol with AAV vectors. The presence of NAbs depends mainly on exposure to the virus in nature and varies drastically according to age, geographic location and health status of the person evaluated.

En los últimos años la terapia génica se ha posicionado como una opción real y segura en el desarrollo de alternativas terapéuticas para la cura y la prevención de diferentes enfermedades. Consiste en la inserción de material genético en un tejido o célula defectuosa, mediante el uso de un vector. Existen varias consideraciones para seleccionar el vector más apropiado, incluyendo el potencial de unión y entrada a la célula diana, la capacidad de transferencia del material genético al núcleo, la habilidad de expresión del inserto y la ausencia de toxicidad. En el panorama actual, los vectores virales más utilizados son los derivados de los virus adenoasociados (AAV). Características como su bioseguridad, baja toxicidad y tropismo selectivo, han posibilitado su evaluación como opción terapéutica en un amplio número de enfermedades monogénicas o complejas. A pesar de sus ventajas, los vectores AAV presentan inconvenientes, siendo el más importante la respuesta inmune del paciente al vector, especialmente la respuesta mediada por anticuerpos neutralizantes (NAb). Los NAb disminuyen la transducción del vector e impiden la expresión del gen que transporta, limitando su aplicación clínica. Por lo tanto, identificar y cuantificar la presencia y actividad de los NAbs, es el primer paso en cualquier protocolo de terapia génica con vectores AAV. La presencia de NAb depende principalmente de la exposición al virus en la naturaleza y varía drásticamente según edad, localización geográfica y estado de salud de la persona evaluada.

Evaluation of the efficacy and safety of text messages targeting adherence to cardiovascular medications in secondary prevention: the txt2heart Colombia randomised controlled trial protocol.

INTRODUCTION: Anti-platelet therapy, angiotensin-converting enzyme inhibitors/angiotensin II receptor blockers, beta-blockers and statins are cost-effective in patients with atherosclerotic cardiovascular diseases (ASCVD) for reducing the risk of ASCVD events. Unfortunately, there is abundant evidence that adherence to these cardiovascular medications is far from ideal. A recent Cochrane review showed a potential beneficial effect of Short Message Service (SMS) interventions on adherence to medication in ASCVD patients. METHODS AND ANALYSIS: The txt2heart study is a pragmatic randomised single-blind controlled trial. The objective is to evaluate the efficacy and safety of an intervention with SMS messages delivered by mobile phones to improve adherence to cardiovascular medications in patients with ASCVD. The intervention consists of behavioural techniques delivered via SMS. The primary outcome is change in blood serum low-density lipoprotein cholesterol levels as an indicator of adherence to statins. Secondary outcomes will include systolic blood pressure as an indicator of adherence to blood-lowering therapies and heart rate as an indicator of adherence to beta-blockers, urine levels of 11-dehydrothromboxane B2, self-reported adherence to cardiovascular medications and rates of cardiovascular death or hospitalisation due to cardiovascular disease. ETHICS AND DISSEMINATION: The study will be performed in compliance with the protocol, regulatory requirements, Good Clinical Practice and ethical principles of the Declaration of Helsinki. The Ethics Committee of Fundación Cardiovascular de Colombia evaluated and approved the trial. The txt2heart Colombia trial aims to provide robust evidence to evaluate whether SMS messages delivered through mobile telephones change the behaviour of Colombian patients who have suffered a cardiovascular event. Trial results will be presented to the local health authorities, and if the intervention is effective and safe, we hope this strategy will be implemented quickly because of its low cost and wide-reaching impact on the population. TRIAL REGISTRATION NUMBER: NCT03098186.

Conocimientos de los estudiantes peruanos de enfermería sobre el virus del papiloma humano / Knowledge of Peruvian nursing students about the Human Papilloma Virus

Objetivo: Determinar el nivel de conocimiento de las estudiantes de enfermería sobre el Virus del Papiloma Humano en una universidad privada en el norte del Perú. Método: Cuantitativa, descriptivo, transversal; la muestra fue censal constituida por 90 estudiantes de enfermería del final de la carrera universitaria, se aplicó un instrumento ya validado internacionalmente con un Alpha de Cronbach de 0,76 de 15 preguntas aplicadas vía cuestionario Google por la emergencia sanitaria; se tuvo en consideración los criterios de inclusión y exclusión establecidos. Resultados: El nivel de conocimiento general de los estudiantes universitarios de enfermería fue muy bueno (43.3%). el 92.2% del total fueron mujeres y el 7.8% fueron varones; el 71.4% de varones y 41.0% de mujeres han tenido más de una pareja sexual. El 75% de estudiantes de enfermería que fueron de la selva reportan más de una pareja sexual, contrario al 33.8% de estudiantes de enfermería de la costa reportan más de una pareja sexual. Conclusión: El conocimiento que predominó entre las estudiantes universitarias de enfermería fue bueno y muy bueno; sin embargo, llama la atención que la mayoría tuvo más de una pareja sexual situación que conlleva a desarrollar programas para fomentar la concientización de la salud sexual responsable y libre de riesgos entre las futuras enfermeras. (AU)

Objective: To determine the level of knowledge of nursing students about the Human Papilloma Virus in a Private University in northern Peru. Method: Quantitative, descriptive, cross-sectional; the sample was census constituted by 90 nursing students at the end of the university career, an instrument already validated internationally was applied with a Cronbach's Alpha of 0.76 of 15 questions applied via Google questionnaire for the health emergency; the established inclusion and exclusion criteria were taken into consideration. Results: The level of general knowledge of the university nursing students was very good (43.3%). 92.2% of the total were women and 7.8% were men; 71.4% of men and 41.0% of women have had more than one sexual partner. 75% of nursing students who went from the jungle report more than one sexual partner, contrary to 33.8% of nursing students from the coast report more than one sexual partner. Conclusion: The knowledge that prevailed among the university nursing students was good and very good; however, it is noteworthy that the majority had more than one sexual partner, a situation that leads to the development of programs to promote awareness of responsible and risk-free sexual health among future nurses. (AU)

Vitamin D and risk of preeclampsia: A systematic review and meta-analysis.

Introduction: Human vitamin D levels have been increasingly related to a wide range of clinical outcomes. There is a large amount of reports on its associations, especially with obstetric complications, including preeclampsia and gestational diabetes. These results are scarcely consistent and there is still a lack of quality intervention studies to confirm the role of vitamin D in those outcomes. Objective: To review the available scientific evidence on the role of maternal vitamin D in the development of preeclampsia. Materials and methods: The methodology used followed the recommendations of the Cochrane guide for the preparation of systematic reviews, and for metaanalysis, the Guide of the Metaanalysis of Observational Studies in Epidemiology group (MOOSE). The search included both observational studies and controlled clinical trials. Results: Low vitamin D levels, measured by the 25-hydroxyvitamin D test, are common in pregnancy. The results of this systematic review and metaanalysis suggest an inverse ratio between vitamin D levels and the development of preeclampsia. There was heterogeneity among the studies with regard to the design, population, geographic location, definitions of exposure, and the outcome. We excluded randomized controlled trials from this meta-analysis. Conclusion: The inverse association we found suggests that the higher the levels of vitamin D the lesser the probability of developing preeclampsia, in spite of the heterogeneity of the global measurement in this type of analysis.

Introducción: Cada vez son más los hallazgos sobre la relación entre las concentraciones de vitamina D en el ser humano y diversas condiciones clínicas. Hay una gran cantidad de estudios que informan sobre dicha asociación, especialmente con complicaciones obstétricas, incluidas la preeclampsia y la diabetes mellitus de la gestación, entre otras, pero sus resultados todavía no son definitivos, por lo que se requieren estudios de intervención de calidad que confirmen la relación de la vitamina D con dichos resultados. Objetivo: Revisar la información plasmada en estudios en torno al papel de la vitamina D materna y el desarrollo de la preeclampsia. Materiales y métodos: La metodología usada siguió las recomendaciones de la guía Cochrane para la elaboración de revisiones sistemáticas y de la guía del grupo Meta-analysis of Observational Studies in Epidemiology (MOOSE) para los metaanálisis. La búsqueda incluyó estudios observacionales y ensayos clínicos controlados. Resultados: Los niveles bajos de vitamina D, medida con el examen de 25-hidroxivitamina D, son comunes en el embarazo. Los resultados de esta revisión sistemática y del metaanálisis sugieren una asociación inversa entre los niveles de vitamina D y el desarrollo de preeclampsia. Hubo heterogeneidad en los estudios en cuanto a su diseño, población y ubicación geográfica, así como a las definiciones de exposición y resultado. Los ensayos clínicos controlados aleatorizados se excluyeron del metaanálisis. Conclusión: Se encontró una asociación inversa que sugiere que, a mayores concentraciones de vitamina D, menor es la probabilidad de desarrollar preclampsia, a pesar de la heterogeneidad de la medida global en este tipo de análisis.

Cost-effective Evaluation of an Educational Intervention based on Multiple Intelligences Versus Traditional Care to Improve Exclusive Breastfeeding in Mothers' Clubs in Peru

Objective. This work sought to evaluate the cost-effectiveness of two educational interventions to improve exclusive maternal breastfeeding in mothers' clubs in Peru. Methods. This is a retrospective, longitudinal descriptive study, which reviewed 113 information registries of mothers participating in a traditional educational intervention and 104 mothers with intervention based on multiple intelligences, evaluating the level of knowledge and exclusive maternal breastfeeding practices through observation visits. Results. The intervention based on multiple intelligences achieved greater cost-effectiveness than the traditional intervention given that with lower investment, it managed to get 56.73% of the mothers to increase their knowledge and 68.27% to practice exclusive maternal breastfeeding from six months to more months in comparison with the traditional intervention in which 41.59% and 43.36% improved, respectively. Moreover, for its effective application, on every 100 mothers, its investment would result lower than the traditional program. Conclusion. Upon applying satisfactorily the cost-effectiveness evaluation, as model to compare educational interventions on exclusive maternal breastfeeding, better results were determined with the intervention based on multiple intelligences compared with the traditional intervention, given that with lower investment, it was possible to benefit a greater number of mothers in their level of knowledge and practices of maternal breastfeeding.

Objetivo. Evaluar el costo-efectividad de dos intervenciones educativas para mejorar la lactancia materna exclusiva en clubes de madres en Perú. Métodos. Es un estudio descriptivo, retrospectivo y longitudinal. Se hizo revisión de 113 registros de información de madres participantes de una intervención educativa tradicional y 104 madres con intervención basada en inteligencias múltiples, evaluando el nivel de conocimiento y las prácticas de lactancia materna exclusiva mediante visitas de observación. Resultados. La intervención basada en inteligencias múltiples consiguió mayor costo-efectividad que la intervención tradicional debido a que, con menor inversión, se logró que el 56.73% de las madres incrementaran sus conocimientos y el 68.27% practicaran lactancia materna exclusiva de seis a más meses en comparación a la intervención tradicional en la cual el 41.59% y 43.36% mejoraran respectivamente. Además, para su aplicación efectiva, sobre cada cien madres, su inversión resultaría menor que el programa tradicional. Conclusión. Al aplicar satisfactoriamente la evaluación costo-efectividad como modelo para la comparación de intervenciones educativas sobre lactancia materna exclusiva, se determinaron mejores resultados con la intervención basada en inteligencias múltiples en comparación con la intervención tradicional, pues, con una menor inversión, se logró beneficiar a una mayor cantidad de madres en su nivel de conocimientos y prácticas de lactancia materna.

Objetivo. Avaliar o custo-efetividade de duas intervenções educacionais para melhorar o aleitamento materno exclusivo em clubes de mães no Peru. Métodos. Estudo descritivo, retrospectivo e longitudinal, baseado na revisão de 113 fichas de informações de mães participantes de uma intervenção educativa tradicional e 104 mães com uma intervenção baseada em inteligências múltiplas, avaliando o nível de conhecimentos e práticas da amamentação exclusiva por meio de visitas de observação. Resultados. A intervenção baseada em múltiplas inteligências obteve maior custo-efetividade do que a intervenção tradicional, pois com menor investimento conseguiu que 56.73% das mães aumentassem seus conhecimentos e 68.27% praticasse o aleitamento materno exclusivo por 6 meses ou mais, em comparação com a intervenção tradicional em que 41.59% e 43.36% melhoraram respectivamente; além disso, para sua aplicação efetiva a cada cem mães, seu investimento seria inferior ao do programa tradicional. Conclusão. Ao aplicar com sucesso a avaliação de custo-efetividade como modelo de comparação de intervenções educativas em aleitamento materno exclusivo, foram determinados melhores resultados a favor da intervenção baseada em múltiplas inteligências, em comparação à intervenção tradicional, constatando que, com menor investimento, A maior número de mães foi beneficiado em seu nível de conhecimento e práticas de amamentação.

Asociación VACTERL con poliquistosis renal en un recién nacido: reporte de caso

Las malformaciones son causas importantes de mortalidad infantil, enfermedad crónica y discapacidad en muchos países, y ocasionan 3,2 millones de discapacidades al año. La asociación VACTERL involucra defectos vertebrales, atresia anal, fístula traqueoesofágica y displasia renal, así como anormalidades en las extremidades. Nuestro objetivo es describir las características generales de la asociación VACTERL y realizar un acercamiento a otros casos en la literatura. Nuestro caso trata de una recién nacida a las 35 semanas con grave dificultad respiratoria, que ingresa a la UCI neonatal por descompensación hemodinámica. Tiene un antecedente de poliquistosis renal bilateral a las 25 semanas dado por ecografía. En el examen físico se evidencia agenesia de órganos sexuales y ano imperforado, y en la radiografía se observa patrón atelectásico, cardiomegalia y posición dextrógira de la silueta cardiaca. La paciente fue diagnosticada con asociación VACTERL y ano imperforado. Sufrió un paro respiratorio y falleció luego de dos días.

Malformations are important causes of infant mortality, chronic disease and disability in many countries, causing 3.2 million disabilities per year. The VATERL association includes vertebral defects, anal atresia, tracheoesophageal fistula and renal dysplasia, as well as limb abnormalities. This case report aims to provide the general characteristics of the VACTERL association and discuss other cases found in the medical literature. This is the case of a female newborn delivered at 35 weeks of pregnancy with severe respiratory distress, who was admitted to the neonatal ICU due to hemodynamic decompensation. She had a history of bilateral polycystic kidney disease found by ultrasound at 25 weeks of pregnancy. A physical examination showed vaginal agenesis and imperforate anus. Atelectasis, cardiomegaly and dextroposition of the cardiac silhouette were observed in a radiography. The patient was diagnosed with VACTERL association and imperforate anus. She died 2 days after a respiratory arrest.

Rabdomiosarcoma paratesticular: Reporte de caso y revisión de la literatura / Paratesticular rhabdomyosarcoma: Case report and literature review

Objetivo:Ofrecer un alcance de la relevancia del diagnóstico oportuno del cáncer testicular, a partir de un caso infrecuente de rabdomiosarcoma paratesticular en un adulto. Se trata de un paciente varón Reporte de Caso:de 39 años, que ingresa al servicio de cirugía - urología por un aumento de volumen de contenido escrotal, de forma progresiva desde hace 2 años, con crecimiento paulatino de la masa. En la ecografía testicular se evidencia un notorio aumento de volumen de bolsas escrotales por aparente tumoración o masa de consistencia dura, de estructura heterogénea y aspecto sólido de bordes mal definidos. Considerando los hallazgos clínicos, marcadores testiculareseimagenológicossediagnosticóuntumorparatesticular derecho. Se realizó una orquiectomía derecha más exéresis de tumor paratesticular derecho. Patología concluyó en rabdomiosarcoma embrionario paratesticular con cápsula rota. Paciente fue dado de alta con seguimiento por consulta externa y referido a hospital oncológico de cuarto nivel.

Objetive:Offer a scope of the relevance of the timely diagnosis of testicular cancer, based on a infrequent case of paratesticular rhabdomyosarcoma in an adult.The patient is a 39-year-old male who was admitted to Case report:the urology-surgery department because of an increase in scrotal volume, which has been progressive over the past two years, with gradual growth of the mass. Testicular ultrasound shows a notorious increase in volume of scrotal bags due to an apparent tumour or mass of hard consistency, of heterogeneous structure and solid aspect with ill-defined edges. Considering the clinical findings, testicular markers and imaging, a right paratesticular tumor was diagnosed. Aright orchiectomy plus right paratesticular tumor exeresis was performed. Pathology concluded in paratesticular embryonic rhabdomyosarcoma with a broken capsule. Patient was discharged with outpatient follow-up and referred to the fourth level cancer hospital

Rabdomiosarcoma paratesticular: Reporte de caso y revisión de la literatura / Paratesticular rhabdomyosarcoma: Case report and literature review

RESUMEN Objetivo: Ofrecer un alcance de la relevancia del diagnóstico oportuno del cáncer testicular, a partir de un caso infrecuente de rabdomiosarcoma paratesticular en un adulto. Reporte de Caso: Se trata de un paciente varón de 39 años, que ingresa al servicio de cirugía - urología por un aumento de volumen de contenido escrotal, de forma progresiva desde hace 2 años, con crecimiento paulatino de la masa. En la ecografía testicular se evidencia un notorio aumento de volumen de bolsas escrotales por aparente tumoración o masa de consistencia dura, de estructura heterogénea y aspecto sólido de bordes mal definidos. Considerando los hallazgos clínicos, marcadores testiculares e imagenológicos se diagnosticó un tumor paratesticular derecho. Se realizó una orquiectomía derecha más exéresis de tumor paratesticular derecho. Patología concluyó en rabdomiosarcoma embrionario paratesticular con cápsula rota. Paciente fue dado de alta con seguimiento por consulta externa y referido a hospital oncológico de cuarto nivel.

ABSTRACT Objetive: Offer a scope of the relevance of the timely diagnosis of testicular cancer, based on a infrequent case of paratesticular rhabdomyosarcoma in an adult. Case report: The patient is a 39-year-old male who was admitted to the urology-surgery department because of an increase in scrotal volume, which has been progressive over the past two years, with gradual growth of the mass. Testicular ultrasound shows a notorious increase in volume of scrotal bags due to an apparent tumour or mass of hard consistency, of heterogeneous structure and solid aspect with ill-defined edges. Considering the clinical findings, testicular markers and imaging, a right paratesticular tumor was diagnosed. A right orchiectomy plus right paratesticular tumor exeresis was performed. Pathology concluded in paratesticular embryonic rhabdomyosarcoma with a broken capsule. Patient was discharged with outpatient follow-up and referred to the fourth level cancer hospital.

Factores intervinientes para la ejecución del presupuesto de donaciones y transferencias en el Hospital Regional Lambayeque -Perú / Intervening factors for the execution of the budget for donations and transfers at the Lambayeque Regional Hospital - Perú

Objetivo: Determinar los factores intervinientes para la ejecución del presupuesto de donaciones y transferencias en el Hospital Regional Lambayeque. Material y Métodos: No experimental, descriptivo, enfoque cuantitativo, corte transversal, durante el 2019. Población y muestra fue 57 colaboradores, muestreo no probabilístico, por conveniencia. Se utilizó instrumento Oria divididos en: factor institucional, factor político, factor organizacional y factor personal, la confiabilidad mediante alfa de Cronbach; además se usó la prueba de Kolmogorov-Sminorv para la normalidad de la población; y para la significancia estadística el análisis descriptivo. Hallazgos: el nivel de intervención medio 3,444 para la variable factores intervinientes siendo el factor más resaltante el Político (media 3,7222) y el ítem más resaltante (84%) referido al papeleo de los procesos administrativos, retrasa la ejecución presupuestal de gastos. Conclusiones: Existe un nivel de intervención medio de los factores intervinientes para la ejecución de presupuesto de donaciones y transferencias del hospital Regional Lambayeque.

Objective: To determine the factors involved in the implementation of the donation and transfer budget at the Lambayeque Regional Hospital. Material and Methods: Not experimental, descriptive, quantitative approach, cross-section, during 2019. Population and sample was 57 collaborators, non-probabilistic sampling, for convenience. The Oria instrument was divided into : institutional factor, political factor, organizational factor and personal factor, reliability by Cronbach alpha; in addition, the Kolmogorov- Sminorv test was used for the normality of the population; and for statistical significance the descriptive analysis. Findings: the average intervention level of 3,444 for the variable factors involved being the most outstanding factor the Politician (average 3,7222) and the most outstanding item (84%) referring to the paperwork of the administrative processes, delays the budgetary execution of expenses. Conclusions: There is a medium level of intervention of the factors involved in the execution of the donation and transfer budget of the Lambayeque Regional Hospital.