RESUMO
Autophagy is an evolutionary conserved self-balancing process that plays an important role in maintaining cellular homeostasis via the clearance of damaged organelles and misfolded proteins. Infection-triggered autophagy specifically inhibits the invasion of intracellular bacterial replication and hence protects the cells from microbial infections. It has been reported that Acinetobacter baumannii trigger cell autophagy. However, the role of its virulence protein OmpA remains unclear. Therefore, this study aimed to explore the effects of Acinetobacter baumannii OmpA on cell autophagy and its underlying molecular mechanisms. The results showed that OmpA induced autophagy in HeLa and RAW264.7 cells, increased LC3BII expression, and hindered p62 degradation. Moreover, OmpA triggered incomplete autophagy by interfering the fusion of autophagosomes with lysosomes. Besides, OmpA activated MAPK/JNK signaling pathway and enhanced the phosphorylation levels of JNK, p38, and ERK, c-Jun. Inhibition of JNK signaling pathway suppressed OmpA-induced autophagy in HeLa cells. Ab wild-type strains carrying OmpA triggered incomplete autophagy and resulted in a large number of IL-1ß production. Ab-â³OmpA strain (OmpA gene mutation) restored autophagic flux and reduced the accumulation of p62 and the release of IL-1ß in HeLa cells. Rapamycin activated autophagy to inhibit OmpA-induced IL-1ß secretion and protect HeLa cells from inflammatory damage. Collectively, these results suggest that OmpA can induce autophagy in HeLa cells through MAPK/JNK signaling pathway. Pre-treatment with Rapamycin activates autophagy and protects against cell death.
Assuntos
Acinetobacter baumannii/imunologia , Autofagia , Proteínas da Membrana Bacteriana Externa/metabolismo , Células HeLa/imunologia , Células HeLa/microbiologia , Sistema de Sinalização das MAP Quinases , Animais , Sobrevivência Celular/efeitos dos fármacos , Humanos , Camundongos , Células RAW 264.7 , Sirolimo/metabolismoRESUMO
Lead bismuth eutectic (LBE) alloy shows high potential for application in advanced nuclear systems such as lead-alloy-cooled fast reactors. However, high-temperature LBE liquid is prone to corrode the reference containment material, typically made of steel, through a process known as liquid metal corrosion. In this work, an extensive set of first-principles calculations was performed to investigate the diffusion behavior of steel alloy elements and O in liquid LBE. The results showed Bi atoms diffusing a little bit faster than Pb atoms, and the Ni atoms in steel being most likely to dissolve into the LBE. Compared to Cr atoms, Fe atoms were calculated to diffuse more slowly, and Ni atoms more rapidly. In the presence of Al and/or Si, Al-O and Si-O pairs were calculated to be more stable than Fe-O/Cr-O pairs and to be inclined to form protective stable Al/Si related oxides. The Ni-O distance and pair formation energy in LBE indicated the Ni-O pair to be inclined to decompose over a period of time. We expect these data to be used as indispensable information for understanding the dissolution and oxidation corrosion behavior of steels in liquid LBE.
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Immobilization of enzyme could offer the biocatalyst with increased stability and important recoverability, which plays a vital role in the enzyme's industrial applications. In this study, we present a new strategy to build an intelligent enzyme carrier by coating titania nanoparticles with thermoresponsive epoxy-functionalized polymers. Zero-valent copper-mediated living radical polymerization (Cu(0)-LRP) was utilized herein to copolymerize N-isopropylacrylamide (NIPAM) and glycidyl acrylate (GA) directly from an unprotected dopamine-functionalized initiator to obtain an epoxy-containing polymer with terminal anchor for the "grafting to" or "one-pot" modification of titania nanoparticles. A rhodamine B-labeled laccase has been subsequently used as a model enzyme for successful immobilization to yield an intelligent titania/laccase hybrid bifunctional catalyst. The immobilized laccase has shown excellent thermal stability under ambient or even relatively high temperature above the lower critical solution temperature (LCST) at which temperature the hybrid particles could be facilely recovered for reuse. The enzyme activity could be maintained during the repeated use after recovery and enzymatic degradation of bisphenol A was proven to be efficient. The photocatalytic ability of titania was also investigated by fast degradation of rhodamine B under the excitation of simulated sunlight. Therefore, this study has provided a facile strategy for the immobilization of metal oxide catalysts with enzymes, which constructs a novel bifunctional catalyst that will be promising for the "one-pot" degradation of different organic pollutants.
Assuntos
Catecóis/química , Enzimas Imobilizadas/química , Compostos de Epóxi/química , Lacase/química , Nanopartículas/química , Titânio/química , Acrilamidas/química , Acrilatos/química , Biocatálise , Cobre/química , Enzimas Imobilizadas/metabolismo , Lacase/metabolismo , Rodaminas/químicaRESUMO
Acinetobacter baumannii (A. baumannii) causes autophagy flux disorder by degrading STX17, resulting in a serious inflammatory response. It remains unclear whether STX17 can alter the inflammatory response process by controlling autolysosome function. This study aimed to explore the role of STX17 in the regulation of pyroptosis induced by A. baumannii. Our findings indicate that overexpression of STX17 enhances autophagosome degradation, increases LAMP1 expression, reduces Cathepsin B release, and improves lysosomal function. Conversely, knockdown of STX17 suppresses autophagosome degradation, reduces LAMP1 expression, augments Cathepsin B release, and accelerates lysosomal dysfunction. In instances of A. baumannii infection, overexpression of STX17 was found to improve lysosomal function and reduce the expression of mature of GSDMD and IL-1ß, along with the release of LDH, thus inhibiting pyroptosis caused by A. baumannii. Conversely, knockdown of STX17 led to increased lysosomal dysfunction and further enhanced the expression of mature of GSDMD and IL-1ß, and increased the release of LDH, exacerbating pyroptosis induced by A. baumannii. These findings suggest that STX17 regulates pyroptosis induced by A. baumannii by modulating lysosomal function.
Assuntos
Acinetobacter baumannii , Interleucina-1beta , Lisossomos , Piroptose , Proteínas Qa-SNARE , Lisossomos/metabolismo , Acinetobacter baumannii/metabolismo , Acinetobacter baumannii/genética , Interleucina-1beta/metabolismo , Interleucina-1beta/genética , Humanos , Proteínas Qa-SNARE/metabolismo , Proteínas Qa-SNARE/genética , Proteínas de Ligação a Fosfato/metabolismo , Proteínas de Ligação a Fosfato/genética , Autofagia , Animais , Catepsina B/metabolismo , Catepsina B/genética , Infecções por Acinetobacter/microbiologia , Camundongos , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular/genética , Autofagossomos/metabolismo , Proteína 1 de Membrana Associada ao Lisossomo/metabolismo , GasderminasRESUMO
Background: Microsatellite instability (MSI), or mismatch repair-deficiency (dMMR), is rare in prostate cancers (PCas). The histological and molecular features of PCas with MSI/dMMR are incompletely described. Thus, we sought to identify the characteristics of PCas with MSI/dMMR. Methods and results: We analyzed 1,141 primary treatment-naive PCas by MMR-related protein immunohistochemistry (MLH1, PMS2, MSH2, and MSH6). We identified eight cases exhibiting MSI/dMMR (0.7%, 8/1141). Of these, six tumors had both MSH2 and MSH6 protein loss, one had both MLH1 and PMS2 protein loss, and one had only MSH6 loss. Histologically, MSI/dMMR-PCas frequently demonstrated high histological grade (Grade Group 4 or 5), ductal/intraductal histology (6/8 cases), pleomorphic giant-cell features (4/8 cases), and conspicuous tumor lymphocytic infiltration (8/8 cases). Polymerase chain reaction-based analysis of seven MSI/dMMR tumors revealed two MSI-H tumors with loss of both MSH2 and MSH6 proteins. Subsequently, the seven cases underwent next-generation sequencing (NGS) analysis with a highly validated targeted panel; four were MSI. All cases had a high tumor mutation burden (median: 45.3 mutations/Mb). Overall, the MSI/dMMR-PCas showed a high frequency of DNA damage-repair pathway gene changes, including five with pathogenic somatic or germline MMR gene mutations. Activating mutations in the MAPK pathway, PI3K pathway, and WNT/ß-catenin pathway were common. TMPRSS2::ERG rearrangement was identified in one case (1/7, 14.3%). Conclusions: Several pathological features are associated with MSI/dMMR in PCas. Identification of these features may help to select patients for genetic screening. As MSI/dMMR-PCas are enriched for actionable mutations, patients should be offered NGS to guide standard-of-care treatment.
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OBJECTIVE: To investigate the molecular mechanism of one patient with abnormal serological phenotype in RhD and discuss the transfusion strategy. METHODS: The RhD variant sample was screened from a patient with IgM type anti-D antibody and further determined by three different sources of anti-D antibodies. Ten exons and the adjacent introns of the RHD gene were amplified, purified and sequenced. RhCE phenotypes and RHCE genotypes were detected. RESULTS: The patient with Rh variant showed abnormal results of serological tests. The RHD gene sequence analysis showed that the RHD*01W.01 with a variation (c.809T>G, p.Val270Gly) in exon 6 of the RHD gene was found in the patient. The RhCE phenotype was CcEe. The genotyping results of RHCE were consistent with the serological typing results. CONCLUSION: The Rh variant of the patient is RHD*01W.01, these findings indicate that RhD variants should be analyzed by molecular assays for the sake of safe transfusion.
Assuntos
Transfusão de Sangue , Sistema do Grupo Sanguíneo Rh-Hr , Alelos , Éxons , Genótipo , Humanos , Fenótipo , Sistema do Grupo Sanguíneo Rh-Hr/genéticaRESUMO
Acinetobacter baumanniitriggers autophagy, affects the degradation of autophagy, and causes severe inflammatory injury. LncRNA growth arrest-specific transcript 5 (LncRNA-GAS5) and Yin and Yang 1 (YY1) are known to play an important role in the regulation of autophagy, however, the precise role of LncRNA-GAS5 and YY1 in the damage to autophagy caused by Acinetobacter baumanniiremains unclear. The aim of this study was to investigate the role of LncRNA-GAS5 and YY1 in the regulation of autophagy induced by Acinetobacter baumannii. We found that LncRNA-GAS5 was up-regulated following infection with Acinetobacter baumannii, thus resulting in the degradation of STX17, autophagy disorders, and the aggravated replication of Acinetobacter baumannii. We also analyzed the mechanism of interaction between LncRNA-GAS5 and YY1 and found that YY1 regulated its expression in a negative manner by binding to the promoter of LncRNA-GAS5. LncRNA-GAS5 and YY1 had opposite effects on the expression of STX17, this process maintained the stable expression of STX17. Following Acinetobacter baumannii infection, YY1 was down regulated and then separated from the binding region of LncRNA-GAS5, thus resulting in the activation of LncRNA-GAS5 transcription and reduction in STX17 protein expression. Finally, we infected LncRNA-GAS5 knockdown mice with Acinetobacter baumannii, the expression levels of IFN-ß in the lungs increased significantly, this alleviated lung injury. In conclusion, our work demonstrated the mechanism by which Acinetobacter baumannii infection can cause the degradation of STX17. We also demonstrated that LncRNA-GAS5 may be a potential therapeutic target for the treatment of lung injury induced by Acinetobacter baumannii.
Assuntos
Infecções por Acinetobacter/genética , Lesão Pulmonar , Proteínas Qa-SNARE/metabolismo , RNA Longo não Codificante , Fator de Transcrição YY1/metabolismo , Acinetobacter baumannii , Animais , Autofagia , Lesão Pulmonar/microbiologia , Camundongos , Regiões Promotoras Genéticas , RNA Longo não Codificante/genéticaRESUMO
Sewage sludge and bagasse were used as raw materials to produce cheap and efficient adsorbent with great adsorption capacity of Pb(2+). By pyrolysis at 800 °C for 0.5 h, the largest surface area (806.57 m(2)/g) of the adsorbent was obtained, enriched with organic functional groups. The optimal conditions for production of the adsorbent and adsorption of Pb(2+) were investigated. The results of adsorb-ability fitted the Langmuir isotherm and pseudo-second-order model well. The highest Pb(2+) (at pH = 4.0) adsorption capacity was achieved by treating with 60% (v/v) HNO3. This is a promising approach for metal removal from wastewater, as well as recycling sewage sludge and bagasse to ease their disposal pressure.
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Celulose/química , Carvão Vegetal/química , Chumbo/isolamento & purificação , Saccharum/química , Poluentes Químicos da Água/isolamento & purificação , Purificação da Água/métodos , Adsorção , Resíduos Industriais/prevenção & controle , Íons/isolamento & purificação , Chumbo/química , Esgotos/química , Ultrafiltração/métodos , Águas Residuárias , Poluentes Químicos da Água/químicaRESUMO
BACKGROUND: Suppressor of cytokine signaling (SOCS) proteins are inhibitors of cytokine signaling pathway involved in negative feedback loops. Although SOCS1 is an important intracellular suppressor of apoptosis in a variety of cell types, its role in cytokine-induced pancreatic ß-cell apoptosis remains unclear. The present study investigated potential effects of SOCS1 on the cytokine-induced pancreatic ß-cell apoptosis. METHODS: After successfully transfected with SOCS1/pEGFP-C1 or pEGFP-C1 plasmids to overexpress SOCS1, RINm5F (rat insulinoma cell line) cells were exposed to cytokines, interferon (IFN)-γ alone, IFN-γ+interleukin (IL)-1ß, IFN-ß+IL-1ß+tumor necrosis factor (TNF)-α respectively. Pancreatic ß-cell apoptosis was assessed by using MTT, FACS, and caspase-3 activity assays. Protein phosphorylation of Janus kinase 2 (JAK2) and signal transducers and activators of transcription 1 (STAT1) were verified by Western blotting and mRNA expression of inducible nitric oxide synthase (iNOS), NF-κB and Fas were analyzed by RT-PCR. RESULTS: Overexpression of SOCS1 in RINm5F cells was shown to attenuate IFN-γ alone, IFN-γ+IL-1ß and IFN-γ+TNF-α+IL-1ß mediated apoptosis. Phosphorylation of JAK2 and STAT1 significantly decreased in RINm5F cells which overexpressed SOCS1 protein. Overexpression of SOCS1 significantly suppressed cytokine-induced iNOS mRNA levels. CONCLUSION: Overexpression of SOCS1 protects pancreatic islets from cytokine-induced cell apoptosis via the JAK2/STAT1 pathway.