A new corrosion method (Aycan's method).

BACKGROUND: As it is known, the anatomy of the vessels is examined by removing the cast of the vessels inside the organs. Generally, liquid material (polyester, takilon, etc.) is injected into the vessels with positive pressure to remove the cast from the vessels of the organs. We built a machine to remove the cast of the vessels inside the organ. We named it corrosion machine. MATERIALS AND METHODS: Sheep kidneys were used in our experiment. After the kidneys were cannulated, they were placed in the vacuum chamber. With the operation of the vacuum pump, negative pressure was created in the vacuum chamber. With negative pressure, kidneys and its vessels expanded. Takilon or polyester easily entered the vessels of the kidney. The cast of the vessels of the kidney was removed. With this newly developed technique, the anatomy of the vessels whose casts were removed was examined with the naked eye, stereomicroscope or SEM. RESULTS: The corrosion machine we built can cast the vessels of the organs very well. Takilon or polyester (which we used in our experiment) easily entered the capillaries under the effect of negative pressure. CONCLUSIONS: We think that this method can also be applied to other organs and used in vascular research.

An anatomical and volumetric study on brain ventricles in sheep using different techniques.

This study investigated the morphological features of the ventricular system of the brain in Akkaraman sheep in a measured and structural manner. In the study, 24 adults male Akkaraman sheep, which is a common and hornless breed were used. Anatomical features of the ventricular system were determined by latex injection and dissection, acrylic injection and corrosion cast and magnetic resonance imaging. In the measurements, a precise digital calliper, Archimedes' principle, Cavalieri's principle and ITK-SNAP imaging were used by comparing them with each other. The average weight of fresh sheep brains was 102 g (0.226 lb), the volume 115.8 mL and the length, height and width were 130.78, 40.58 and 70.08 mm, respectively. The olfactoric bulb contained a cavity associated with the lateral ventricle, large enough to be called a ventricle. The cavity of septum pellucidum has a large cavity was observed. However, there was no cerebrospinal fluid in this cavity. The interventricular foramen of the dissected brain was also an opening measuring 1.4 mm × 3.9 mm × 5.5 mm. The total volume of the ventricles of the brain of Akkaraman sheep was found to be 7107.71 ± 479, 7115 ± 737 and 7080 ± 647 mm3 and 6200 mm3 using the Planimetry, Point Counting method, ITK-SNAP program, and Archimedes' principle, respectively. In the study, species and breed-specific brain indices values and many detailed morphometric data were obtained.

A new perspective on the corneo-scleral junction with three types of microscopy techniques.

The conjunctions of the cornea and sclera in the eyes of donkeys, cattle, dogs, sheep, pigs and rabbits, regardless of gender, were examined in this study. No animals were specifically sacrificed for this investigation. Scanning electron microscopy, light microscopy, and dissecting microscopy were used in this research. In the limbus of all the animals investigated, the cornea and sclera fused in accordance with a pattern. At the corneo-scleral junction, the sclera was situated anteriorly and the cornea posteriorly in the dorsal and ventral sections of the bulbus oculi. In the medial and lateral parts of the eyeball, the cornea and sclera were facing each other and interlaced. Pigmentation and the sulcus scleralis externus could be used to identify the macro-and micro-anatomical boundaries of the limbus. In addition, the cytoplasm of basal epithelial cells shrank, signaling the end of the corneal epithelium and the start of the conjunctival epithelium. The presence of Bowman's membrane in cattle and sheep eyes was definitely determined in histological examinations. Bowman's membrane in these animals came to an end at the limbus, which is where the conjunctival epithelium starts and the corneal epithelium ends. In all areas of the cornea, Bowman's membrane revealed irregular, abrupt thickening and thinning. The corneal epithelium was thick in the vertex and thinner towards the limbus, whereas Descemet's membrane was thin in the center (vertex) and thick in the periphery (near the limbus). In this study, pictures and diagrams were used to illustrate the general anatomical, histological, and morphometric characteristics of the limbus in the species under investigation. The data from our study showed that the limbus region of the bulbus oculi was narrow in the lateral and medial parts and wide in the dorsal and ventral parts. This was confirmed in the studied animals as a general rule. The width value will undoubtedly affect the number of cells covered by the regions. It is conceivable that these cells will play a significant role in the decision of where to perform surgical procedures in order to promote wound healing, giving doctors an advantage. In this circumstances, we think that the limbus should be studied in terms of clinical methods because it has different shapes depending on the species and the position of the bulbus.

Pericardial fluid and vascular tissue engineering: A preliminary study.

BACKGROUND: The heart is surrounded by a membrane called pericardium or pericardial cavity. OBJECTIVE: In this study, we investigated the pericardial fluid (PF) for coating polycaprolactone (PCL) scaffolds. PFS, which is a PF component, was used for the coating material. In addition to using PFS for surface coating, MED and fetal bovine serum (FBS) were also used for comparison. METHODS: Pericardial fluid cells (PFSc) isolated from PF were cultured on coated PCL scaffolds for 1, 3, and 5 days. Cell viability was determined using 3-(4, 5-di-methylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The MTT assay results showed that the viability of cells on PCL scaffold coated with PFS increased over time (P < 0.005), and cell viability was significantly different between PCL scaffolds coated with PFS and non-coated PCL scaffolds. However, cell viability was significantly higher in the PCL scaffolds coated with PFS than non-coated and coated with FBS, MED, and PCL scaffolds. Scanning electron microscopy (SEM) microscopy images and MTT assay indicated that PFSc are attached, proliferated, and spread on PCL scaffolds, especially on PCL scaffolds coated with PFS. CONCLUSIONS: These results suggest that PFS is a biocompatible material for surface modification of PCL scaffolds, which can be used as a suitable material for tissue engineering applications.

Campylobacter anatolicus sp. nov., a novel member of the genus Campylobacter isolated from feces of Anatolian Ground Squirrel (Spermophilus xanthoprymnus) in Turkey.

Seventy-four Gram-negative, motile, slightly curved rod-shaped, microaerophilic, oxidase-positive and catalase-negative isolates, recovered from fecal samples of the Anatolian ground squirrel (Spermophilus xanthoprymnus) in Kayseri, Turkey, were subjected to a polyphasic taxonomic study. Results of a genus-specific PCR indicated that all isolates belonged to the genus Campylobacter. 16S rRNA gene sequence analyses revealed the closest match as Campylobacter curvus DSM 6644T with identity levels of 96.41-96.70%. Based on the 16S rRNA gene phylogeny of the 74 isolates, six isolates (faydin-G24, faydin-G52, faydin-G105, faydin-G114, faydin-G129 and faydin-G140T) were chosen as representatives for further characterization. The overall genome relatedness indices for the strain faydin-G140T, compared to the most closely related type strain C. curvus ATCC 35224T, were calculated as 15.2%, 72.5%, and 83.7% for digital DNA-DNA hybridization (dDDH), and average nucleotide identity (ANIb and ANIm), respectively. The G+C content and genome size of the strains ranged between 35.2-35.4 mol% and 1.7-1.8 Mb, respectively. Based on data obtained from the polyphasic taxonomy approach, including phenotypic characterization as well as genomic and chemotaxonomic analyses, these strains are concluded to represent a novel species, for which the name Campylobacter anatolicus sp. nov. is proposed with faydin-G140T as the type strain (=DSM 112311T = LMG 32238T).

Effects of intraperitoneal administration of the phenytoin on the skeletal system of rat fetus.

This study was conducted on determining the effects of phenytoin on the skeletal system of the fetuses of 13 Wistar Albino rats. The female rats were divided into two groups after the vaginal smear test: the group 1 (control group) included 6 individuals, whereas the group 2 (phenytoin group) comprised 7 animals. A dose of 25mg/kg/day phenytoin was administered intraperitoneally to pregnant rats on the 8th-10th days of pregnancy and fetuses were obtained by C-section on the 20th day. A number of 82 fetuses were observed by double staining technique. Their lengths and weights were measured, revealing the statistically significant differences between the two groups (p<0.001). The lengths of the fetuses in the group 2 were determined as to be 14% shorter and the weights 13% lower compared to those in the group 1. Similarly, number of the fetuses obtained in one gestation decreased 9% in the group 2. Ossification of the skull bones in the fetuses of the group 2 was observed eminently to be deteriorated through using dissection microscope and inspection. Costal separation anomaly was observed in the 10 fetuses of the group 2. The separated-laterally located costal components were not attached to the costal arch. Shape malformations in the last two ribs and wide angularity, particularly in the last six ribs, were also determined. This study has documented that intraperitoneal usage of the pheytoin during pregnancy may cause to different skeletal malformations, even with lower doses, in rat fetuses.