Enhancing Micropropagation of Adenophora liliifolia: Insights from PGRs, Natural Extracts, and pH Optimization.

The endangered plant species Adenophora liliifolia faces threats to its survival in the wild, necessitating the development of effective micropropagation techniques for potential reintroduction efforts. This study demonstrates that Adenophora liliifolia effectively reproduces on MS synthetic medium with diverse plant growth regulators (PGR) and natural extracts, facilitating swift micropropagation for potential future reintroduction endeavors. It highlights the substantial impact of PGR composition and natural extracts on the growth and development of A. liliifolia. The ideal growth medium for A. liliifolia was determined to be ½ MS with specific treatments. Additionally, incorporating silver nitrate (AgNO3) at 5 mg L-1 into the medium led to enhanced root formation and shoot length, albeit excessive concentrations adversely affected root development. Varying concentrations of NAA significantly affected different plant growth parameters, with the 0.1 mg L-1 treatment yielding comparable plant height to the control. Moreover, 50 mL L-1 of coconut water bolstered root formation, while 200 mL L-1 increased shoot formation during in vitro propagation. However, elevated doses of coconut water (CW) impeded root development but stimulated shoot growth. Experiments measuring chlorophyll a + b and carotenoid content indicated higher concentrations in the control group than differing levels of applied coconut water. Optimizing pH levels from 6.8-7 to 7.8-8.0 notably enhanced plant height and root formation, with significant carotenoid accumulation observed at pH 6.8-7. Soil samples from A. liliifolia's natural habitat exhibited a pH of 6.65. Ultimately, the refined in vitro propagation protocol effectively propagated A. liliifolia, representing a pioneering effort and setting the stage for future restoration initiatives and conservation endeavors.

Salt Tolerance of Limonium gmelinii subsp. hungaricum as a Potential Ornamental Plant for Secondary Salinized Soils.

Secondary salinization caused by climate change is a growing global problem. Searching for plants that can survive in areas with high salt content and even have decorative value was the focus of our research. Thirty plants of Limonium gmelinii subsp. hungaricum were planted in clear river sand; another thirty plants were planted in Pindstrup, a growing substrate enriched with 40% clay. With the latter, we modeled the natural soil. In addition to the control tap-water treatment, plants received 50, 125, 250, 375, and 500 mM NaCl solution irrigation twice a week. The leaf sizes of plants planted in sand decreased proportionally with the increasing NaCl concentration, and their dry matter content increased. In the clay-containing medium, leaf sizes increased, even at a concentration of 375 mM, although the dry matter content increased only at high concentrations. Carotene content in both media became higher, due to the higher NaCl concentrations, while proline content in the plants grown in sandy media increased, even with the 125 mM concentration. With our present experiment we proved the salt tolerance of the taxon, and even the soil's great importance in supporting the plant's salt tolerance.

Chrysanthemum: A Comprehensive Review on Recent Developments on In Vitro Regeneration.

Chrysanthemum is a flowering plant grown worldwide and is one of the most popular ornamental plants. Chrysanthemums are usually cultivated using root suckers and shoot cuttings. This conventional technique is relatively slow. In addition, as cuttings are gained regularly from mother plants, there is a chance of viral infection and degeneration, which raises the production cost. The hurdles mentioned above have been managed by applying in vitro propagation techniques, which can enhance reproduction rates through in vitro culture and use very small explants, which are impossible with the conventional approach. Usually, it is difficult to get true-to-type plants as the parents with good quality, but clonal propagation of a designated elite species makes it possible. Hence, this review highlights recent studies of the in vitro propagation of Chrysanthemum included; the appropriate explant sources, medium compositions, alternative disinfection of culture media, plant growth regulators (PGRs), different mutagenesis applications, acclimatization efficiency, and alternative light sources to overcome the shortcomings of conventional propagation techniques.