RESUMO
In the last 5 years, there has been only one reported human case of West Nile virus (WNV) disease in northern Mexico. To determine if the virus was still circulating in this region, equine and entomological surveillance for WNV was conducted in the state of Nuevo Leon in northern Mexico in 2006 and 2007. A total of 203 horses were serologically assayed for antibodies to WNV using an epitope-blocking enzyme-linked immunosorbent assay (bELISA). Seroprevalences for WNV in horses sampled in 2006 and 2007 were 26% and 45%, respectively. Mosquito collections in 2007 produced 7365 specimens representing 15 species. Culex mosquitoes were screened for WNV RNA and other genera (Mansonia, Anopheles, Aedes, Psorophora and Uranotaenia) were screened for flaviviruses using reverse-transcription (RT)-PCR. Two pools consisting of Culex spp. mosquitoes contained WNV RNA. Molecular species identification revealed that neither pool included Culex quinquefasciatus (Say) (Diptera:Culicidae) complex mosquitoes. No evidence of flaviviruses was found in the other mosquito genera examined. These data provide evidence that WNV is currently circulating in northern Mexico and that non-Cx. quinquefasciatus spp. mosquitoes may be participating in the WNV transmission cycle in this region.
Assuntos
Culicidae/virologia , Doenças dos Cavalos/virologia , Cavalos/virologia , Insetos Vetores/virologia , Febre do Nilo Ocidental/veterinária , Vírus do Nilo Ocidental/isolamento & purificação , Animais , Anticorpos Antivirais/sangue , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Doenças dos Cavalos/sangue , Doenças dos Cavalos/epidemiologia , Masculino , México/epidemiologia , Dados de Sequência Molecular , Prevalência , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Análise de Sequência de RNA/veterinária , Homologia de Sequência , Estudos Soroepidemiológicos , Especificidade da Espécie , Febre do Nilo Ocidental/sangue , Febre do Nilo Ocidental/epidemiologia , Febre do Nilo Ocidental/virologiaRESUMO
The distributional area of the tick Ixodes ricinus (L.), the primary European vector to humans of Lyme borreliosis spirochaetes (Borrelia burgdorferi sensu lato) and tick-borne encephalitis virus, appears to be increasing in Sweden. It is therefore important to determine which environmental factors are most useful to assess risk of human exposure to this tick and its associated pathogens. The geographical distribution of I. ricinus in Sweden was analysed with respect to vegetation zones and climate. The northern limit of I. ricinus and B. burgdorferi s.l. in Sweden corresponds roughly to the northern limit of the southern boreal vegetation zone, and is characterized climatically by snow cover for a mean duration of 150 days and a vegetation period averaging 170 days. The zoogeographical distribution of I. ricinus in Sweden can be classified as southerly-central, with the centre of the distribution south of the Limes Norrlandicus. Ixodes ricinus nymphs from 13 localities in different parts of Sweden were examined for the presence of B. burgdorferi s.l. and found to be infected with Borrelia afzelii and Borrelia garinii. Tick sampling localities were characterized on the basis of the density of Borrelia-infected I. ricinus nymphs, presence of specific mammals, dominant vegetation and climate. Densities of I. ricinus nymphs and Borrelia-infected nymphs were significantly correlated, and nymphal density can thus serve as a general indicator of risk for exposure to Lyme borreliosis spirochaetes. Analysis of data from this and other studies suggests that high densities of Borrelia-infected nymphs typically occur in coastal, broadleaf vegetation and in mixed deciduous/spruce vegetation in southern Sweden. Ixodes ricinus populations consistently infected with B. burgdorferi s.l. can occur in: (a) biotopes with shrews, rodents, hares and birds; (b) biotopes with shrews, rodents, hares, deer and birds, and (c) island locations where the varying hare (Lepus timidus) is the only mammalian tick host.
Assuntos
Borrelia burgdorferi , Ixodes , Doença de Lyme/epidemiologia , Infestações por Carrapato/epidemiologia , Animais , Borrelia burgdorferi/genética , Clima , Primers do DNA , Meio Ambiente , Geografia , Aquecimento Global , Humanos , Mamíferos , Densidade Demográfica , Prevalência , Medição de Risco , Neve , Suécia/epidemiologiaRESUMO
Nonremoval drag sampling was conducted in multiple sites in Larimer County, CO, from March to July 2006 to determine the seasonal pattern of host-seeking activity by the human-biting adult life stage of the Rocky Mountain wood tick, Dermacentor andersoni Stiles (Acari: Ixodidae). Four sites, at elevations ranging from 1,790 to 2,470 m, consistently yielded host-seeking ticks. Ticks were active from March until late June; the length of the active period ranged from 84 to 104 d. Based on site-specific linear regression models of increase and decrease in tick host-seeking activity over time, densities of D. andersoni adults were estimated to reach 50% of their peaks between 2 and 11 April, to peak between 21 and 28 April, and to fall below 50% of the peaks between 24 and 27 May. The length of the periods with tick densities exceeding 50 and 75% of the peak was 43-52 and 21-26 d, respectively. Rapid increases in tick numbers in late March to early April followed a stretch of days with daily maximum temperatures exceeding 5 degrees C, whereas rapidly declining tick numbers in mid- to late May were associated with daily maximum temperatures consistently exceeding 20 degrees C and daily minimum relative humidity commonly falling below 20%. Densities of D. andersoni adults exceeded 50% of the peak when daily maximum temperatures were in the 16-19 degrees C range and daily minimum relative humidity was > 20%. Finally, tick seasonality may be adapted to local climatic conditions within Larimer County; site-specific daily maximum temperatures at the time of peak tick host-seeking activity in late April were positively associated with site-specific mean daily maximum temperatures for April.
Assuntos
Mordeduras e Picadas/epidemiologia , Dermacentor/fisiologia , Umidade , Estações do Ano , Temperatura , Animais , Comportamento Apetitivo/fisiologia , Mordeduras e Picadas/parasitologia , Colorado/epidemiologia , Comportamento Alimentar/fisiologia , Interações Hospedeiro-Parasita , Humanos , Densidade Demográfica , Fatores de TempoRESUMO
Arkansas-Missouri has emerged as the primary U.S. focus of tularemia, which is caused by the National Institute of Allergy and Infectious Diseases Category A Priority Pathogen Francisella tularensis, over the past 30 yr. There are several pieces of indirect evidence suggesting that a key role of ticks in the transmission of F. tularensis to humans in Arkansas-Missouri is the primary reason why tularemia has remained a prominent disease of humans in this two-state area while fading away from other central or eastern states after a general decline in rabbit-associated tularemia cases. The primary tick vector(s) in Arkansas-Missouri can, based on a comparison of seasonal patterns of human tularemia cases and peak host-seeking activity of commonly human-biting tick species and life stages, be narrowed down to Amblyomma americanum (L.) nymphs, A. americanum adults, or Dermacentor variabilis (Say) adults. Unfortunately, currently available data cannot be used to further elucidate the relative roles of these ticks as vectors of F. tularensis to humans in Arkansas-Missouri. To address the fact that we do not know which tick species is the primary vector of F. tularensis to humans in the most prominent U.S. focus of tularemia, we need to determine (1) relative contributions of different tick species and life stages as human biters in Arkansas-Missouri; (2) natural rates of infection with F. tularensis tularensis (type A) and F. tularensis holarctica (type B) of the most prominent human-biting ticks in areas of Arkansas-Missouri hyperendemic for tularemia; (3) experimental vector efficiency of these ticks for both F. tularensis tularensis and F. tularensis holarctica; and (4) presence of infection with F. tularensis tularensis or F tularensis holarctica in ticks collected from humans in Arkansas-Missouri.
Assuntos
Francisella tularensis/fisiologia , Doenças Transmitidas por Carrapatos/epidemiologia , Doenças Transmitidas por Carrapatos/microbiologia , Tularemia/epidemiologia , Tularemia/microbiologia , Animais , Arkansas/epidemiologia , Vetores Artrópodes/crescimento & desenvolvimento , Vetores Artrópodes/microbiologia , Dermacentor/crescimento & desenvolvimento , Dermacentor/microbiologia , Humanos , Ixodidae/crescimento & desenvolvimento , Ixodidae/microbiologia , Estágios do Ciclo de Vida , Missouri/epidemiologia , Pesquisa , Estações do Ano , Doenças Transmitidas por Carrapatos/transmissão , Tularemia/transmissãoRESUMO
We exploited an elevation (climate) gradient ranging from 1,700 to 2,500 m in Poudre Canyon of Larimer County, CO, to determine climatic correlates of abundance per 15-s drag sampling time unit (hereafter referred to as abundance) of the human-biting adult life stage of the Rocky Mountain wood tick, Dermacentor andersoni Stiles (Acari: Ixodidae), in a key risk habitat for tick exposure: south/west-facing, rocky hillsides with mixed grass-brush-conifer vegetation. The relationship between elevation and abundance was parabolic, with peak tick abundances occurring at mid-range elevations (2,200-2,400 m) and tick abundances approaching zero at approximately 2,100 and 2,500 m. Regression modeling demonstrated that abundance of host-seeking adult ticks in south/west-facing exposures was accurately predicted by several climate variables related to temperature (e.g., mean annual minimum temperature, maximum temperature, and base 10 degrees C growing degree-days, and median length of annual freeze-free period; r2 values ranging from 0.771 to 0.864), whereas mean annual precipitation, snowfall, or relative humidity were uninformative in this respect (r2 values ranging from 0.020 to 0.316). Abundance of D. andersoni adults peaked at a mean annual maximum temperature of approximately 10 degrees C and a mean annual growing degree-day value of approximately 650. Relationships between climate variables and abundance of D. andersoni adults were used to create geographic information system (GIS)-based models for predicted tick abundance in south/west-facing exposures in Larimer County. This is the first GIS-based model developed for spatial patterns of abundance of D. andersoni. Finally, preliminary data from Poudre Canyon indicate a shift toward peak abundances of D. andersoni adults occurring in sheltered northern/eastern exposures, rather than in drier and hotter southern/ western exposures, at elevations below 2,100 m.
Assuntos
Mordeduras e Picadas/parasitologia , Dermacentor/parasitologia , Altitude , Animais , Clima , Colorado , Dermacentor/crescimento & desenvolvimento , Ecossistema , Geografia , Humanos , Densidade Demográfica , TemperaturaRESUMO
We used drag sampling to examine the geographical distribution patterns of ixodid ticks engaging in open (non-nidicolous) host-seeking behavior in dense woodland habitats of the climatically and ecologically diverse Mendocino County in north coastal California. The findings based on this sampling methodology reflect risk of human exposure to host-seeking ticks rather than the true distribution of the ticks. Drag sampling in 78 sites yielded 7,860 nymphal or adult Ixodes pacificus Cooley & Kohls, 220 Dermacentor occidentalis Marx, 150 Ixodes spinipalpis Hadwen & Nuttall, 15 Hemaphysalis leporispalustris (Packard), 12 Ixodes angustus Neumann, 12 Ixodes auritulus Neumann, and a single Dermacentor variabilis (Say). I. pacificus, which is the primary vector of the Lyme disease spirochete Borrelia burgdorferi to humans in California, occurred in all 78 sites examined. D. occidentalis, another tick species commonly biting humans in California, and H. leporispalustris typically were encountered in oak-associated woodlands in the central or eastern parts of the county. In contrast, three species of Ixodes ticks (I. angustus, I. auritulus, and I. spinipalpis) most commonly were found questing openly in woodlands with redwood present in the western part of the county. I. angustus and I. spinipalpis are occasional human biters and known experimental vectors of B. burgdorferi. Our study represents the first collection of large numbers of openly host-seeking I. spinipalpis ticks. Univariate tests of associations between presence of ticks (D. occidentalis, H. leporispalustris, I. angustus, I. auritulus, or I. spinipalpis) and environmental geographical information systems-remote sensing (GIS/ RS)-based data indicated that elevation, number of growing degree-days, and tasseled cap brightness, greenness, and wetness are especially useful predictors of presence of openly hostseeking ticks. Combinations of the above-mentioned GIS/RS-based data yielded significant logistic regression models for habitat suitability of host-seeking ticks for all five above-mentioned species. The model equations were used to create spatial surfaces of predicted presence of suitable habitat for openly host-seeking ticks in Mendocino County dense woodlands.
Assuntos
Vetores Aracnídeos/fisiologia , Ecossistema , Ixodidae/fisiologia , Animais , Biodiversidade , California , Demografia , Sistemas de Informação Geográfica , Geografia , Modelos Logísticos , ÁrvoresRESUMO
The western fence lizard, Sceloporus occidentalis, is refractory to experimental infection with Borrelia burgdorferi sensu stricto, one of several Lyme disease spirochetes pathogenic for humans. Another member of the Lyme disease spirochete complex, Borrelia bissettii, is distributed widely throughout North America and a similar, if not identical, spirochete has been implicated as a human pathogen in southern Europe. To determine the susceptibility of S. occidentalis to B. bissettii, 6 naïve lizards were exposed to the feeding activities of Ixodes pacificus nymphs experimentally infected with this spirochete. None of the lizards developed spirochetemias detectable by polymerase chain reaction for up to 8 wk post-tick feeding, infected nymphs apparently lost their B. bissettii infections within 1-2 wk after engorgement, and xenodiagnostic L. pacificus larvae that co-fed alongside infected nymphs did not acquire and maintain spirochetes. In contrast, 3 of 4 naïve deer mice (Peromyscus maniculatus) exposed similarly to feeding by 1 or more B. bissettii-infected nymphs developed patent infections within 4 wk. These and previous findings suggest that the complement system of S. occidentalis typically destroys B. burgdorferi sensu lato spirochetes present in tissues of attached and feeding I. pacificus nymphs, thereby potentially reducing the probability of transmission of these bacteria to humans or other animals by the resultant adult ticks.
Assuntos
Grupo Borrelia Burgdorferi/imunologia , Lagartos/imunologia , Lagartos/microbiologia , Doença de Lyme/veterinária , Animais , Vetores Aracnídeos/microbiologia , Proteínas do Sistema Complemento/imunologia , Suscetibilidade a Doenças/veterinária , Feminino , Imunidade Inata/imunologia , Ixodes/microbiologia , Doença de Lyme/imunologia , Doença de Lyme/transmissão , Masculino , Ninfa/microbiologia , PeromyscusRESUMO
The molecular basis for the loss of steroid binding activity in receptorless (r-) glucocorticoid-resistant (dexr) mutants isolated from the glucocorticoid-sensitive (dexs) cell line CEM-C7 was investigated. Although there was little binding of the reversibly associating ligand [3H]dexamethasone in r- mutants, labeling with the covalent affinity ligand [3H] dexamethasone 21-mesylate revealed significant amounts of a 92 kilodalton human glucocorticoid receptor (hGR) protein. Immunoblots of hGR protein in r- and normal cells showed that r- mutants expressed approximately half the amount of immunoreactive hGR protein seen in dexs cells. Comparison of the genomic organization of the hGR genes in normal and mutant cells revealed no discernable differences in the structure, or dosage, indicating that the r- phenotype was not the result of gross deletion or rearrangement of the hGR genes. In addition, r- cells expressed the same 7 kilobase mRNA as normal cells. More importantly, the amount of hGR mRNA expressed in r- cells was never significantly less, and in some cases was greater than, that seen in normal cells, indicating that the decrease in immunoreactive hGR protein seen in r- cells is not the result of loss of hGR mRNA expression. Taken together with the known mutation rate of the hGR gene(s) in these cells, these results suggest that the hGR genes in dexs CEM-C7 cells are allelic and that dexs cells express both a normal hGR protein and one with an altered steroid binding site. Furthermore, they suggest that the r- phenotype is acquired as the result of mutation within the coding region of the originally functional allele, leading to loss of ligand binding and expression of immunoreactive product.
Assuntos
Regulação da Expressão Gênica , Leucemia/genética , Mutação , Receptores de Glucocorticoides/genética , Northern Blotting , Southern Blotting , Genótipo , Humanos , Ligantes , Fenótipo , RNA Mensageiro/análise , Células Tumorais CultivadasRESUMO
Activation of the rat kidney mineralocorticoid receptor was investigated using diethylaminoethyl (DEAE)-cellulose, DNA-cellulose, and gel permeation chromatography. Specific labeling of the mineralocorticoid receptor was achieved by labeling with [3H]aldosterone in the presence of the pure glucocorticoid RU28362. The specificity of labeling was confirmed by the lack of immunoreactivity of [3H]aldosterone-labeled material with the monoclonal antiglucocorticoid receptor antibody BURG-1. The unactivated aldosterone-mineralocorticoid receptor complex did not bind to DNA-cellulose, was eluted from DEAE-cellulose at relatively high salt (195 mM KCl) concentration, and had an apparent Stokes radius when chromatographed on Sephacryl S300 of 6.3 nm. After activation, the aldosterone-mineralocorticoid receptor complex had increased affinity for DNA-cellulose and decreased affinity for DEAE-cellulose and appeared as a smaller complex when chromatographed on Sephacryl S300. These changes were blocked by sodium molybdate. Our results indicate that activation of the rat kidney mineralocorticoid receptor is analogous to activation of the glucocorticoid receptor and suggest that activation of the mineralocorticoid receptor involves dissociation of a multimeric receptor form.
Assuntos
Rim/metabolismo , Receptores de Esteroides/metabolismo , Aldosterona/metabolismo , Androstanóis/farmacologia , Animais , Fenômenos Químicos , Físico-Química , Cromatografia , Cromatografia DEAE-Celulose , Cromatografia em Gel , Citosol/metabolismo , DNA/metabolismo , Masculino , Ratos , Ratos Endogâmicos , Receptores de Mineralocorticoides , Receptores de Esteroides/efeitos dos fármacosRESUMO
Regulation of glucocorticoid receptor (GR) expression by its cognate ligand was examined in the glucocorticoid-sensitive human leukemic T-cell line 6TG1.1 and in the human B-cell line IM-9. In contrast to the decrease in GR mRNA seen in IM-9 cells after treatment with 1 microM dexamethasone for 16-18 h, treatment of 6TG1.1 cells resulted in an 8-fold increase in GR mRNA, as determined by Northern blot and RNase protection analysis, with a corresponding 3- to 4-fold increase in GR protein. Half-maximal induction of GR mRNA and protein in 6TG1.1 cells was observed between 10-100 nM dexamethasone, and inclusion of 1 microM RU 38486 completely blocked the effects of 100 nM dexamethasone, demonstrating that positive autoregulation of GR expression in 6TG1.1 cells is a receptor-mediated response. Positive autoregulation of GR expression was also observed in glucocorticoid-resistant CEM-C1 cells, which contain functional GR, but whose growth is unaffected by glucocorticoids. Thus, positive autoregulation is neither a consequence nor the sole cause of growth arrest. The degree of negative autoregulation in IM-9 cells and positive autoregulation in 6TG1.1 cells was unaffected by inhibition of protein synthesis with cycloheximide. Measurement of GR mRNA turnover in 6TG1.1 cells treated with actinomycin-D revealed a half-life of 2.5 h, which was unaffected by dexamethasone treatment. A similar half-life was determined in IM-9 cells and was also unaffected by steroid treatment. These results are consistent with the interpretation that glucocorticoid-mediated autoregulation of GR expression is a tissue-specific primary transcriptional response.
Assuntos
Linfócitos B/metabolismo , Regulação da Expressão Gênica , Receptores de Glucocorticoides/genética , Linfócitos T/metabolismo , Northern Blotting , Dactinomicina/farmacologia , Dexametasona/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Sondas RNA , RNA Mensageiro/metabolismo , RibonucleasesRESUMO
Fifty-two newborns were assessed for the effects of maternal cocaine use on their performance on the Brazelton Neonatal Behavior Assessment Scale and on their stress behaviors during the Brazelton as tapped by the Neonatal Stress Scale. The cocaine-exposed newborns experienced more obstetric complications, had smaller head circumferences, showed more limited habituation abilities on the Brazelton Scale, and exhibited more stress behaviors than control newborns.
Assuntos
Cocaína/efeitos adversos , Estresse Fisiológico/induzido quimicamente , Peso ao Nascer/efeitos dos fármacos , Feminino , Idade Gestacional , Humanos , Recém-Nascido , Masculino , Troca Materno-Fetal , Perinatologia , Exame Físico , Gravidez , Reflexo Anormal/efeitos dos fármacos , Estresse Fisiológico/fisiopatologiaRESUMO
Many large U.S. companies have transformed their procurement of health benefits in the 1990s by combining the principles of managed competition with other business tactics to create a business-savvy hybrid of the private sector's own design, often referred to as "value purchasing." Until recently, few policymakers or health care observers believed that large firms would be a force in health system reform. Yet to implement value purchasing, the large companies in this study created new organizational forms, provided employees with financial incentives to select low-cost health plans, and used business tactics such as competitive bidding to negotiate more favorable rates and to improve quality among health plans. The financial results were impressive for the companies studied. In addition, the companies' demands on the health care delivery system are multiplying as the interface between business firms and health care organizations changes. These demands will only increase as the practices we found become more widespread.
Assuntos
Compras em Grupo/economia , Planos de Assistência de Saúde para Empregados/economia , Programas de Assistência Gerenciada/economia , Competição em Planos de Saúde/economia , Participação da Comunidade , Proposta de Concorrência , Serviços Contratados , Tomada de Decisões , Setor de Assistência à Saúde/tendências , Humanos , Setor Privado , Estados UnidosRESUMO
BACKGROUND: Intestinal intussusception in the adult is a rare entity that differs greatly in etiology from its pediatric counterpart. Controversy remains regarding the optimal management of this problem in the adult patient. The purpose of this study was to determine the cause(s) of intussusception and to determine the role of intestinal reduction in the management of intussusception in adults. STUDY DESIGN: A retrospective review performed at The Mount Sinai Medical Center identified 27 patients, 16 years and older, with a diagnosis of intestinal intussusception. Data related to presentation, diagnosis, treatment, and pathology were analyzed. RESULTS: There were 13 males and 14 females. The median age of the group was 52 years with a range of 16 to 90 years. Abdominal pain was the most common presenting complaint. A preoperative diagnosis was suspected in 11 of 27 patients (40%). There were 22 small bowel lesions and 5 colonic lesions. A pathologic cause was identified in 85% of patients with 8 of 22 (36%) small bowel and 4 of 5 (80%) of large bowel lesions being malignant. All small bowel cancers represented metastatic disease and all large bowel malignancies were primary adenocarcinomas. The median age of patients with malignant disease was 60 years; it was 44 years for those with benign disease. Operative treatment consisted of resection alone in 58% of patients and resection after reduction in 42%. Three patients were treated nonoperatively. CONCLUSIONS: Our data support a selective approach to the operative treatment of intussusception in adults. Colonic lesions should not be reduced before resection because they most likely represent a primary adenocarcinoma. Small bowel intussusception should be reduced only in patients in whom a benign diagnosis has been made preoperatively or in patients in whom resection may result in short gut syndrome.
Assuntos
Intussuscepção/cirurgia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Intussuscepção/diagnóstico , Masculino , Pessoa de Meia-Idade , Estudos RetrospectivosRESUMO
An acarologic study was conducted in a semirural community in northern California to determine the relative abundance of, and the prevalence of infection with, three emerging bacterial pathogens in the western black-legged tick (Ixodes pacificus). These included the agents causing Lyme disease (Borrelia burgdorferi), human granulocytic ehrlichiosis [Ehrlichia phagocytophila (formerly Ehrlichia equi)], and human monocytic ehrlichiosis (Ehrlichia chaffeensis). The study area in Sonoma County consisted of two properties each with four residents and an uninhabited adjacent comparison area. Six of the eight residents had been either physician-diagnosed or serodiagnosed previously with Lyme disease, and, of these, one also had been serodiagnosed with human monocytic ehrlichiosis. Direct immunofluorescent/culture assays and bacterial species-specific polymerase chain reaction assays were used to test whole ticks individually for presence of B. burgdorferi and Ehrlichia spp., respectively. Overall, 6.5% of the nymphal (n = 589) and 1.6% of the adult ticks (n = 318) from the same generational cohort were found to contain B. burgdorferi. In contrast, none of 465 nymphs and 9.9% of 202 adults were infected with E. phagocytophila. Excised tissues from another 95 adult ticks yielded a comparable E. phagocytophila infection prevalence of 13.7%. E. chaffeensis was not detected in either nymphal or adult ticks. Using a combination of culture and polymerase chain reaction assays, coinfection of I. pacificus adults with B. burgdorferi and E. phagocytophila was demonstrated for the first time. The marked disparity in the infection prevalence of these pathogens in nymphal and adult ticks suggests that their maintenance cycles are inherently different.
Assuntos
Anaplasma phagocytophilum/isolamento & purificação , Vetores Aracnídeos/microbiologia , Borrelia burgdorferi/isolamento & purificação , Ehrlichia chaffeensis/isolamento & purificação , Ixodes/microbiologia , Anaplasma phagocytophilum/genética , Animais , Borrelia burgdorferi/genética , California/epidemiologia , DNA Bacteriano/análise , Ehrlichia chaffeensis/genética , Ehrlichiose/epidemiologia , Ehrlichiose/microbiologia , Humanos , Doença de Lyme/epidemiologia , Doença de Lyme/microbiologia , Ninfa/microbiologia , Reação em Cadeia da Polimerase , Prevalência , Fatores de RiscoRESUMO
Drag sampling is a commonly used method to obtain relative estimates of the density of questing nymphal Ixodes scapularis Say and I pacificus Cooley & Kohls ticks, which are primary vectors of Lyme disease spirochetes to humans in North America. However, the efficiency of drag sampling in determining absolute population densities of questing nymphs has not been evaluated previously. Therefore, we assessed the efficiency of a single drag-sampling occasion to estimate the total population size of questing I pacificus nymphs in a leaf-litter habitat in California. Repeated daily removal sampling was carried out in four areas, each covering 300 m2, on 17 occasions over a 23-d period in the spring of 1999. In total, 573 I. pacificus nymphs were collected, of which 55 (9.6%) were collected on the initial sampling occasion and 20 (3.5%) on the last occasion. The total population size of questing nymphs, i.e., the intersection with the horizontal axis of a linear regression of daily nymphal catch rates on the number of nymphs caught previously, was estimated to be 936. Thus, the efficiency of the initial sampling occasion to estimate the total population size was 5.9% (4.8, 5.0, 5.8, and 9.1%, respectively, for the four individual sampling areas). Further, the overall mean efficiencies of the two, five, and 10 first removal sampling occasions to estimate the absolute nymphal density was 5.2, 4.7, and 4.3%, respectively, and 13 sampling occasions were required to collect 50% of the estimated total nymphal population.
Assuntos
Ixodes , Folhas de Planta/parasitologia , Animais , Densidade Demográfica , Reprodutibilidade dos Testes , Estudos de AmostragemRESUMO
This study evaluates the effects of neonatal behavioral assessments and heelsticks on transcutaneous oxygen tension (TcPO2) in 37 preterm neonates from a neonatal intensive care unit. Two behavioral assessments were evaluated, the Brazelton Neonatal Behavioral Assessment Scale (BNBAS) and the Neurobehavioral Assessment for Preterm Infants (NAPI). TcPO2 during heelsticks was used as a comparison because reliable decreases have typically been noted during heelsticks. Although the heelsticks resulted in significant declines in TcPO2, the behavioral assessments led to only negligible changes in TcPO2. Observed changes during the behavioral assessments occurred in a small number of infants, primarily during the few test procedures administered to the infants outside of their isolettes. Accordingly, it is suggested that time outside the isolette and associated temperature changes may be more critical variables than the assessments per se.
Assuntos
Monitorização Transcutânea dos Gases Sanguíneos/métodos , Coleta de Amostras Sanguíneas , Doenças do Prematuro/diagnóstico , Doenças do Sistema Nervoso/diagnóstico , Exame Neurológico , Nível de Alerta/fisiologia , Manobra Psicológica , Humanos , Hipóxia/sangue , Hipóxia/diagnóstico , Recém-Nascido , Doenças do Prematuro/sangue , Unidades de Terapia Intensiva Neonatal , Doenças do Sistema Nervoso/sangue , Fases do Sono/fisiologiaRESUMO
The prevalence and abundance of immature Ixodes pacificus ticks on western fence lizards (Sceloporus occidentalis) were examined in relation to time of year, host attributes (i.e., age, gender, and presence or absence of blood parasites), and 5 environmental characteristics, including topographic exposure and ground cover substrate, over a 2-year period in northern California. Lizards were infested with subadult ticks from early March until late July or early August, with peak median numbers of larvae and nymphs recorded in late April and early May of both years. Peak larval and nymphal abundances differed between years. The overall ratio of larvae to nymphs on adult male lizards was low, ranging from 0.80 in 1999 to 2.41 in 2000. Such intensive feeding of nymphs versus larvae on these lizards, which are reservoir-incompetent for Borrelia burgdorferi spirochetes, may explain previous observations of decreasing spirochetal infection prevalence from the nymphal to adult stage in northwestern California. Adult male lizards were more likely to be infested with nymphs and harbored greater abundances of larvae and nymphs than adult females. Lizards uninfected with blood parasites had more nymphs than infected lizards. The measured environmental characteristics could explain only a small percentage of the total variation observed in larval prevalence (22%) and in larval and nymphal abundance (12 and 3%, respectively).
Assuntos
Ixodes , Lagartos/parasitologia , Infestações por Carrapato/veterinária , Animais , California/epidemiologia , Feminino , Geografia , Larva , Masculino , Ninfa , Prevalência , Estações do Ano , Fatores Sexuais , Infestações por Carrapato/epidemiologiaRESUMO
The density of, and prevalence of infection with Borrelia burgdorferi in, Ixodes pacificus nymphs as well as the density of infected nymphs were compared at 12 properties at a small rural community at high risk for Lyme disease (CHR) and at 12 areas at the University of California Hopland Research and Extension Center (HREC), Mendocino County, California. The mean infection prevalence and density of infected nymphs were 1.7% (range, 0-4.2%) and 0.10 infected nymphs per 100 m2 (range, 0-0.23 per 100 m2) at the HREC, and 12.4% (range, 3.9-41.3%) and 1.83 infected nymphs per 100 m2 (range, 0.29-22.17 per 100 m2) at the CHR. Thus, the mean density of infected nymphs differed 18-fold between CHR and HREC and 76-fold between properties at the CHR. Also, there was up to 10-fold variation in infection prevalence and 16-fold variation in density of infected nymphs between discrete areas within properties at the CHR. The high densities of infected nymphs recorded at the CHR suggest that, despite the low statewide incidence of Lyme disease, the medical community should be alerted that Lyme disease can be highly endemic in rural areas of northwestern California. The prevalence of spirochetal infection was higher for nymphs collected in southern/western, as compared to northern/eastern, exposures at both HREC and CHR. Infection prevalence and nymphal density were negatively associated at the HREC, whereas they tended to be associated positively at the CHR. A positive association was observed between nymphal density and density of infected nymphs when data from CHR and HREC were combined, and when data from the CHR were considered alone, but not for data from the HREC alone.
Assuntos
Vetores Aracnídeos/microbiologia , Grupo Borrelia Burgdorferi/fisiologia , Ixodes/microbiologia , Animais , Vetores Aracnídeos/crescimento & desenvolvimento , Grupo Borrelia Burgdorferi/isolamento & purificação , California/epidemiologia , Cervos , Geografia , Ixodes/crescimento & desenvolvimento , Doença de Lyme/epidemiologia , Doença de Lyme/transmissão , Ninfa/crescimento & desenvolvimento , Ninfa/microbiologia , Prevalência , Fatores de RiscoAssuntos
Acidentes de Trânsito , Pacientes Internados , Enfermeiras e Enfermeiros , Pacientes , Feminino , HumanosRESUMO
Color correction in planar optics configurations can be achieved by resorting to gradient-index rather than uniform-refractive-index substrates. The basic configuration, principle of correction, and calculated and experimental results are presented. The results reveal that, with an appropriate refractive index distribution along the thickness of the substrates, the color can be corrected over a wavelength range up to 155 nm depending on incidence angles.