RESUMO
DNA origami structures can be programmed into arbitrary shapes with nanometer scale precision, which opens up numerous attractive opportunities to engineer novel functional materials. One intriguing possibility is to use DNA origamis for fully tunable, targeted, and triggered drug delivery. In this work, we demonstrate the coating of DNA origami nanostructures with virus capsid proteins for enhancing cellular delivery. Our approach utilizes purified cowpea chlorotic mottle virus capsid proteins that can bind and self-assemble on the origami surface through electrostatic interactions and further pack the origami nanostructures inside the viral capsid. Confocal microscopy imaging and transfection studies with a human HEK293 cell line indicate that protein coating improves cellular attachment and delivery of origamis into the cells by 13-fold compared to bare DNA origamis. The presented method could readily find applications not only in sophisticated drug delivery applications but also in organizing intracellular reactions by origami-based templates.
Assuntos
Proteínas do Capsídeo/química , DNA/administração & dosagem , Proteínas Imobilizadas/química , Nanoestruturas/química , Transfecção , Proteínas do Capsídeo/metabolismo , DNA/química , DNA/genética , Sistemas de Liberação de Medicamentos , Células HEK293 , Humanos , Proteínas Imobilizadas/metabolismo , Modelos Moleculares , Conformação de Ácido NucleicoRESUMO
The self-assembly of silver nanoparticles into a bow-tie antenna configuration is achieved with the DNA origami method. Instead of complicated particle geometries, spherical silver nanoparticles are used. Formation of the structures in high yields is verified with transmission electron microscopy and agarose gel electrophoresis. According to finite-difference time-domain simulations, the antenna configuration could be used as a DNA sensor.
Assuntos
Nanopartículas Metálicas/química , Prata/química , Simulação por Computador , DNA/química , DNA/ultraestrutura , Ensaio de Desvio de Mobilidade Eletroforética , Nanopartículas Metálicas/ultraestrutura , Fatores de TempoRESUMO
Degradable Newkome-type and polylysine dendrons functionalized with spermine surface units are used to control the formation of DNA origami structures. The intact dendrons form polyelectrolyte complexes with the scaffold strands, therefore blocking the origami formation. Degradation of the dendron with an optical trigger or chemical reduction leads to the release of the DNA scaffold and efficient formation of the desired origami structure. These results provide new insights towards realizing responsive materials with DNA origami.