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1.
Scand J Med Sci Sports ; 31(3): 521-528, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33179319

RESUMO

This study analyzes the strategy used by the best male runners who participated in one of the major city marathons (Frankfurt Marathon, 2008-2018), the all-time performances <2:04:00, the male world records achieved during the 21st century and the Nike Breaking2 Project and INEOS 1:59 Challenge (total = 235 races). The races of the best runners in the Frankfurt Marathon (top 10) were analyzed (n = 110 runners, range: 2:03:42-2:14:05 hours); the runners were divided into two groups according to the tactical used. The pace of Group A (stable pace) remained steady throughout the race, while in Group B (decrease in running speed toward the end of the race) a moderate, but significant drop in speed was detected (P ≤ .001), starting from halfway through the race and getting sharper from the 30th kilometer (30-35 km = 1.6%, P ≤ .001 - 35-40 km = 4.3%, P ≤ .001 - 40-42.195 km: 3.9%, P ≤ .001, total = ≈10%). In the races in which the world record is achieved, the running speed tends to be steady and relatively conservative during the first stretch of the race, increasing smoothly in the second half and achieving a significant increase in the last 2195 m of the race (P = .016, ES = 1.14). Among all the possible strategies, running at a steady pace throughout the race seems the most effective option, especially when priority is given to time rather than position (ie, world records and best all-time races).


Assuntos
Comportamento Competitivo/fisiologia , Corrida de Maratona/fisiologia , Corrida de Maratona/psicologia , Resistência Física/fisiologia , Metabolismo Energético , Humanos , Masculino
2.
Dev Neurorehabil ; 26(1): 18-26, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36191252

RESUMO

This study aimed to examine the acute changes in dynamic balance Postural Control experienced by children with Autism Spectrum Disorder (ASD) who undertook a 6-month extracurricular Service-Learning Physical Education (PE) program. The study used a quasi-experimental design with 23 participants divided into an experimental group and a control group. Limits of Stability protocol was used to measure the children's postural control. The results showed that the experimental group achieved statistically significant improvements. To conclude, this study provides substantial input about how extracurricular PE activities aimed at developing the general motor proficiency of ASD children can improve their dynamic balance.


Assuntos
Transtorno do Espectro Autista , Humanos , Criança , Educação Física e Treinamento , Equilíbrio Postural , Exercício Físico
3.
Metabolites ; 13(4)2023 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-37110219

RESUMO

Pre-pregnancy obesity and excessive gestational weight gain (GWG) appear to affect birth weight and the offspring's risk of obesity and disease later in life. However, the identification of the mediators of this relationship, could be of clinical interest, taking into account the presence of other confounding factors, such as genetics and other shared influences. The aim of this study was to evaluate the metabolomic profiles of infants at birth (cord blood) and 6 and 12 months after birth to identify offspring metabolites associated with maternal GWG. Nuclear Magnetic Resonance (NMR) metabolic profiles were measured in 154 plasma samples from newborns (82 cord blood samples) and in 46 and 26 of these samples at 6 months and 12 months of age, respectively. The levels of relative abundance of 73 metabolomic parameters were determined in all the samples. We performed univariate and machine-learning analysis of the association between the metabolic levels and maternal weight gain adjusted for mother's age, Body Mass Index (BMI), diabetes, diet adherence and infant sex. Overall, our results showed differences, both at the univariate level and in the machine-learning models, between the offspring, according to the tertiles of maternal weight gain. Some of these differences were resolved at 6 and 12 months of age, whereas some others remained. Lactate and leucine were the metabolites with the strongest and longest association with maternal weight gain during pregnancy. Leucine, as well as other significant metabolites, have been associated in the past with metabolic wellness in both general and obese populations. Our results suggest that the metabolic changes associated to excessive GWG are present in children from early life.

4.
Front Psychol ; 13: 787346, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35369159

RESUMO

Research examining Service-Learning (SL) in Physical Education Teacher Education (PETE) is ample. However, long-term investigations are still scarce and literature demands the application of this type of design to uncover the effects of SL on the long run. This study followed a longitudinal quantitative approach; thus, the participants completed the Civic Attitudes and Skills Questionnaire (CASQ) in three occasions (pretest-postest1-postest2). Results show that there exist significant differences between mean values of the global outcomes of the CASQ; concretely, there was an improvement in the first interval followed by a decrease in the second period. Regarding the different dimensions of the CASQ, leadership skills, attitudes towards social justice and attitudes towards diversity showed significant differences too. This research leads towards better understanding of methodological strategies promoting quality education, positing SL as an adequate possibility in this respect, also in the long term.

5.
Artigo em Inglês | MEDLINE | ID: mdl-35954532

RESUMO

Active and democratic citizenship promotion has become a critical challenge for higher education, and civic engagement is a fundamental axis not only in education but also in fostering democratic systems. Consequently, teacher educators held a prominent role through their own teaching practices to generate contexts promoting critical thinking, skills and attitudes. The aim of this study was to analyze the learning related to the political dimension developed by pre-service teachers (n = 123) after participating in a Service-Learning program through physical education with children at risk or/and student with educational needs. This research followed a qualitative research approach and we based the analysis of reflective diaries on Gorham's (2005) categories regarding political learning: Critical political thinking, Interest in politics, Deliberation and Political judgment. The findings show a development in learning such as civic attitudes, critical political thinking, awareness of social justice problems, increased civic compromise and responsibility. The Service-Learning program, therefore, may have been an adequate option to develop pre-service teachers' learning related to a political perspective. Therefore, these findings let us understand how Service-Learning may foster equity and social justice among future teachers.


Assuntos
Aprendizagem , Educação Física e Treinamento , Atitude , Criança , Humanos , Estudantes , Pensamento
6.
Eur J Sport Sci ; 22(9): 1335-1342, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34256680

RESUMO

Endurance exercise induces notable acute hormonal responses on the gonadal and adrenal hormones. The purpose of this study was to assess the changes in salivary testosterone (Ts), salivary cortisol (Cs) and T/C ratio during long-distance triathlon. Ten well-trained male triathletes participated in the study and were assessed for hormonal changes at four time-points (pre-competition, post-swimming, post-cycling, and post-running phases). Ts decreased from pre-competition to post-swimming (from 93.37 pg/mL to 57.63 pg/mL; p < .01) and increased during two other parts of the competition to almost pre-competition values (cycling: 79.20 pg/mL, p = .02; running: 89,66 pg/mL, p = .04, respectively). Cs showed a similar behaviour; decreasing in the post-swimming phase (1.74 pg/mL) and increasing in the other transitions (post-cycling: 7.30 pg/mL; post-running: 13.31 pg/mL), with significant differences between pre-competition and post- competition values (p = .01). Conversely, T/C increased significantly from pre-competition to post-swimming phase (p = .04) to later decrease until the end of the competition. Overall, T/C significantly decreased (p < .05). In conclusion, during an Ironman triathlon, hormone values fluctuate in response to the demands of the competition. Ts and Cs decrease after-swimming, increase after-cycling and reach the maximum values after-running. T/C reflects overall catabolic status.


Assuntos
Hidrocortisona , Corrida , Ciclismo/fisiologia , Humanos , Masculino , Resistência Física/fisiologia , Corrida/fisiologia , Natação/fisiologia , Testosterona
7.
PLoS One ; 16(6): e0253750, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34166459

RESUMO

BACKGROUND: Numerous studies have reported a gradual decline in the practice of physical exercise with age, confirming high dropout rates in the adolescent period. The aim of this study was to investigate the motivation or barriers that lead to greater or lesser adherence to physical exercise among children and adolescents. METHODS: In this study, 1,081 individuals volunteered to participate [239 children (age: 10.89 ± .66 years) and 842 adolescents (age: 14.9 ± 1.75 years)]. For this purpose, we used the Self-Report on Motivation for Exercising (AMPEF) and Self-Report on Barriers to Exercising (ABPEF) questionnaires. RESULTS: The most significant motivational factors in the practice of physical exercise (in both children and adolescents) are competition, social recognition and challenge (t = -16.02, p < .001), prevention and positive health (t = 5.24, p < .001) and affiliation, fun and well-being (t = -19.54, p < .001), while the barriers focus on fatigue and laziness (t = -13.20, p < .001) and body image and physical-social anxiety (t = -5.71, p < .001). It is also observed that adolescents showed significantly higher motivation than children and greater presence of barriers in the practice of physical exercise. CONCLUSIONS: The age is a determining variable to include in preventive and intervention programs for the practice of physical exercise.


Assuntos
Exercício Físico/psicologia , Fadiga , Motivação , Inquéritos e Questionários , Adolescente , Criança , Fadiga/fisiopatologia , Fadiga/psicologia , Feminino , Humanos , Masculino
8.
Artigo em Inglês | MEDLINE | ID: mdl-34068915

RESUMO

As classrooms become more and more diverse, it is imperative to provide physical education teacher education (PETE) students with opportunities to develop competencies that promote quality education for all students. In this study, PETE students applied a physical education service-learning (SL) program aimed at enhancing Autism Spectrum Disorder (ASD) children's motor domain and general well-being-objectives that are connected to the third focus of the United Nations' Sustainable Development Goals (SDGs). Traditionally, research on SL has focused on students' outcomes, and there is a call to examine SL's effects on service receivers, which is the gap this paper aspires to fill. The aim of this study was to measure the postural control of children with ASD who were involved in a 6-month SL program in comparison to ASD peers in a control group. A quasi-experimental design was used in which a total of 29 children with ASD participated. The results of the experimental group showed a significant improvement in the vestibular pathways, an improvement trend in the somatosensorial and visual pathways and improvements in the dynamic tests. This study provides valuable feedback about how SL programs can benefit ASD children to improve their postural control, thus contributing to the third SDG concerned with well-being promotion.


Assuntos
Transtorno do Espectro Autista , Capacitação de Professores , Criança , Humanos , Aprendizagem , Educação Física e Treinamento , Equilíbrio Postural
9.
Anal Bioanal Chem ; 396(6): 2023-9, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20087729

RESUMO

Several techniques have been developed for detection and quantification of genetically modified organisms, but quantitative real-time PCR is by far the most popular approach. Among the most commonly used real-time PCR chemistries are TaqMan probes and SYBR green, but many other detection chemistries have also been developed. Because their performance has never been compared systematically, here we present an extensive evaluation of some promising chemistries: sequence-unspecific DNA labeling dyes (SYBR green), primer-based technologies (AmpliFluor, Plexor, Lux primers), and techniques involving double-labeled probes, comprising hybridization (molecular beacon) and hydrolysis (TaqMan, CPT, LNA, and MGB) probes, based on recently published experimental data. For each of the detection chemistries assays were included targeting selected loci. Real-time PCR chemistries were subsequently compared for their efficiency in PCR amplification and limits of detection and quantification. The overall applicability of the chemistries was evaluated, adding practicability and cost issues to the performance characteristics. None of the chemistries seemed to be significantly better than any other, but certain features favor LNA and MGB technology as good alternatives to TaqMan in quantification assays. SYBR green and molecular beacon assays can perform equally well but may need more optimization prior to use.


Assuntos
DNA de Plantas/genética , Plantas Geneticamente Modificadas/química , Plantas Geneticamente Modificadas/genética , Reação em Cadeia da Polimerase/métodos , DNA de Plantas/química , Reação em Cadeia da Polimerase/economia
10.
J AOAC Int ; 92(3): 765-72, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19610365

RESUMO

A multiplex polymerase chain reaction assay coupled to capillary gel electrophoresis for amplicon identification by size and color (multiplex PCR-CGE-SC) was developed for simultaneous detection of cotton species and 5 events of genetically modified (GM) cotton. Validated real-time-PCR reactions targeting Bollgard, Bollgard II, Roundup Ready, 3006-210-23, and 281-24-236 junction sequences, and the cotton reference gene acp1 were adapted to detect more than half of the European Union-approved individual or stacked GM cotton events in one reaction. The assay was fully specific (<1.7% of false classification rate), with limit of detection values of 0.1% for each event, which were also achieved with simulated mixtures at different relative percentages of targets. The assay was further combined with a second multiplex PCR-CGE-SC assay to allow simultaneous detection of 6 cotton and 5 maize targets (two endogenous genes and 9 GM events) in two multiplex PCRs and a single CGE, making the approach more economic. Besides allowing simultaneous detection of many targets with adequate specificity and sensitivity, the multiplex PCR-CGE-SC approach has high throughput and automation capabilities, while keeping a very simple protocol, e.g., amplification and labeling in one step. Thus, it is an easy and inexpensive tool for initial screening, to be complemented with quantitative assays if necessary.


Assuntos
Eletroforese Capilar/métodos , Gossypium/genética , Plantas Geneticamente Modificadas/genética , Reação em Cadeia da Polimerase/métodos , Reprodutibilidade dos Testes
11.
Artigo em Inglês | MEDLINE | ID: mdl-31881707

RESUMO

A total of 852 adolescents between 12 and 17 years of age were evaluated (M = 14.86, SD = 1.67), randomly selected among a population of secondary school and Baccalaureate students. We applied an "ad hoc" questionnaire on sociodemographic data and the Self-Report of Reasons for the Practice of Physical Exercise (AMPEF) and Self-Report of Barriers to the Practice of Physical Exercise (ABPEF) questionnaires. The results obtained show a prevalence of competition, social recognition, and challenge as motivational factors, and fatigue or laziness as barriers to physical exercise among adolescents. With respect to gender, boys show a greater presence of motivations towards doing physical exercise, while girls show more barriers. In conclusion, the motivational factors and barriers interact and support each other for a better predisposition towards physical exercise.


Assuntos
Comportamento do Adolescente , Exercício Físico , Motivação , Instituições Acadêmicas , Adolescente , Criança , Feminino , Humanos , Masculino , Fatores Sexuais , Estudantes , Inquéritos e Questionários
12.
PLoS One ; 13(7): e0200690, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30011320

RESUMO

The aim of this study was to examine the impacts on blood lactate concentration, measured heart rate and assessment of perceived exertion during split sessions of equal relative load, as also their relationship to the specific sport practised. Nineteen regional-level athletes (nine middle and long-distance runners (cyclic group) and ten field-sport team players (acyclic group)) performed four high-intensity interval training (HIIT) sessions with work-interval durations of 10 s, 50 s, 90 s and 130 s. The sessions were carried out at their usual training sites with a separation of at least 48 hours. Blood lactate concentration was measured at rest and 3 min after the completion of each protocol. Heart rate was measured continuously during all sessions with a sampling rate of 1 s, and rating of perceived exertion (RPE) was requested at the end of the trial. The results showed an increase in blood lactate concentration, peak heart rate and rating of perceived exertion during long protocols as compared with short ones. No differences were observed in dependent variables between cyclic and acyclic groups. Significant but moderate correlations were observed between post-exercise blood lactate concentration, peak heart rate and RPE.


Assuntos
Atletas , Frequência Cardíaca/fisiologia , Ácido Láctico/sangue , Corrida/fisiologia , Adulto , Feminino , Humanos , Masculino , Fatores de Tempo
13.
J Agric Food Chem ; 55(11): 4312-8, 2007 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-17488028

RESUMO

The most widely accepted methods for accurate quantitative detection of genetically modified organisms rely on real-time PCR. Various detection chemistries are available for real-time PCR. They include sequence-unspecific DNA labeling dyes such SYBR-Green I and the use of both universal (e.g., AmpliFluor) and sequence-specific double-labeled probes, the latter comprising hybridization (e.g., Molecular Beacon) and hydrolysis (e.g., TaqMan or MGB) probes. Also, new real-time PCR devices and reagents allowing fast cycling reactions exist. Five Mon810 real-time PCR assays were developed in which the event specificity was based on the detection of transgene and plant rearranged sequences found to 3' flank the insertion site. Every assay was specifically designed for one particular detection chemistry, that is, AmpliFluor, Molecular Beacon, MGB, TaqMan, and SYBR-Green I. When possible, the assays were adapted to fast cycling mode. All assays displayed satisfactory performance parameters, although Molecular Beacon, MGB, and TaqMan chemistries were the most suitable for quantification purposes in both conventional and fast cycling modes.


Assuntos
DNA de Plantas/análise , Plantas Geneticamente Modificadas/química , Reação em Cadeia da Polimerase/métodos
14.
Podium (Pinar Río) ; 17(3): 1225-1242, sept.-dic. 2022. graf
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1406300

RESUMO

RESUMEN Introducción: En este estudio, se examinó la idoneidad de seleccionar atletas élite desde una edad temprana. Objetivo: El objetivo de este estudio consistió en verificar si el comportamiento identificado en investigaciones antecedentes también ocurre entre los mejores atletas mundiales de todos los tiempos que compiten en la carrera de 100 metros. Materiales y métodos: Para ello, y con base en un análisis de las clasificaciones oficiales de todos los tiempos masculinos y femeninos de la Asociación Internacional de Federaciones de atletismo en la referida prueba, se utilizó muestras en categoría absoluta (Top-1,000 hasta el 31/12/2018) y categoría sub-20 (Top-100 hasta el 31/12/2003); se aplicaron leyes de potencia y otras estrategias típicas de los sistemas complejos para analizar los datos. Resultados: los resultados muestran cómo, con demasiada frecuencia, los mejores corredores masculinos y femeninos del mundo en la categoría sub-20 no evolucionan positivamente durante el resto de su carrera deportiva mejorando sus registros en las categorías superiores. Solo 51 hombres de los 100 mejores corredores sub-20 de todos los tiempos, lograron mejorar sus registros una vez que alcanzaron la categoría sénior, y las mejoras no presentaron siempre progresiones estadísticamente significativas. En consecuencia, una inversión excesiva en recursos humanos y materiales para identificar individuos con altas capacidades atléticas, no es siempre la mejor estrategia. Conclusiones: el deporte de nivel a edades tempranas puede ser un error metodológico que debe evitarse a la hora de construir las reservas deportivas de un país o federación deportiva.


RESUMO Introdução: Neste estudo, examinou-se a adequação da seleção de atletas de elite desde tenra idade. Objetivo: O objetivo deste estudo foi verificar se o comportamento identificado em pesquisas anteriores também ocorre entre os melhores atletas do mundo de todos os tempos que competem na prova de 100 metros. Materiais e métodos: Para isso, e com base na análise das classificações oficiais de todos os tempos para homens e mulheres da Associação Internacional de Federações de Atletismo na prova supracitada, foram utilizadas amostras em categoria absoluta (Top-1.000 até 31/12 /2018) e sub-20 (Top-100 até 31/12/2003); Leis de potência e outras estratégias típicas de sistemas complexos foram aplicadas para analisar os dados. Resultados: Os resultados mostram como, muitas vezes, os melhores corredores masculinos e femininos do mundo na categoria sub-20 não evoluem positivamente durante o resto de sua carreira esportiva, melhorando seus registros nas categorias superiores. Apenas 51 homens dos 100 melhores corredores sub-20 de todos os tempos conseguiram melhorar seus tempos quando chegaram à categoria sênior, e as melhorias nem sempre mostraram progressões estatisticamente significativas. Consequentemente, um investimento excessivo em recursos humanos e materiais para identificar indivíduos com altas habilidades atléticas nem sempre é a melhor estratégia. Conclusões: o esporte de nível em idade precoce pode ser um erro metodológico que deve ser evitado na construção das reservas esportivas de um país ou federação esportiva.


ABSTRACT Introduction: In this study, the suitability of selecting elite athletes from an early age was examined. Objective: The objective of this study was to verify if the behavior identified in previous research also occurs among the world's best athletes of all time who compete in the 100-meter race. Materials and methods: For this, and based on an analysis of the official classifications of all times for men and women of the International Association of Athletics Federations in the referred test, samples were used in absolute category (Top-1,000 up to the 12/31/2018) and sub -20 category (Top - 100 until 12/31/2003); power laws and other strategies typical of complex systems will be applied to analyze the data. Results: The results show how, all too often, the best male and female runners in the world in the under-20 category do not evolve positively during the rest of their sports career. improving their records in the higher categories. Only 51 men out of the top 100 U-20 runners of all time managed to improve their times once they reached the senior category, and the improvements did not always show statistically significant progressions. Consequently, an excessive investment in human and material resources to identify individuals with high athletic abilities is not always the best strategy. Conclusions: level sport at an early age can be a methodological error that should be avoided when building the sports reserves of a country or sports federation.

15.
J AOAC Int ; 89(5): 1347-52, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-17042186

RESUMO

A real-time polymerase chain reaction (QPCR) assay was developed for quantitative detection of a genetically modified (GM) maize event TC-1507 and modification to conventional PCR for qualitative purposes. Sequences 5'-flanking TC-1507 full-length insert were characterized and showed multiple rearrangements involving insert and maize chloroplast fragments. The event specificity of the TC-1507 assays was based on the detection of transgene and plant rearranged sequences found to 5' flank the insertion site. They were fully specific and exhibited a limit of detection below 10 target copies, allowing consistent detection of 0.1% GM levels. The QPCR was highly linear and efficient and proved adequate for quantification of GM contents, aiming at the fulfillment of legal requirements established in the European Union (i.e., compulsory labeling of TC-1507 levels >0.9%). It satisfactorily determined TC-1507 contents on different matrixes and was successfully transferred a different laboratory.


Assuntos
Plantas Geneticamente Modificadas/genética , Reação em Cadeia da Polimerase/métodos , Zea mays/genética , Sequência de Bases , Primers do DNA/genética , DNA de Plantas/genética , União Europeia , Laboratórios , Reação em Cadeia da Polimerase/normas , Reação em Cadeia da Polimerase/estatística & dados numéricos , Sensibilidade e Especificidade
16.
J Agric Food Chem ; 53(18): 7003-9, 2005 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-16131102

RESUMO

Quality assurance is a major issue in the food industry. The authenticity of food ingredients and their traceability are required by consumers and authorities. Plant species such as barley (Hordeum vulgare), rice (Oryza sativa), sunflower (Helianthus annuus), and wheat (Triticum aestivum) are very common among the ingredients of many processed food products; therefore the development of specific assays for their specific detection and quantification are needed. Furthermore, the production and trade of genetically modified lines from an increasing number of plant species brings about the need for control within research, environmental risk assessment, labeling/legal, and consumers' information purposes. We report here the development of four independent real-time polymerase chain reaction (PCR) assays suitable for identification and quantification of four plant species (barley, rice, sunflower, and wheat). These assays target gamma-hordein, gos9, helianthinin, and acetyl-CoA carboxylase sequences, respectively, and were able to specifically detect and quantify DNA from the target plant species. In addition, the simultaneous amplification of RALyase allowed bread from durum wheat to be distinguished. Limits of detection were 1 genome copy for barley, sunflower, and wheat and 3.3 copies for rice real-time PCR systems, whereas limits of quantification were 10 genome copies for barley, sunflower, or wheat and approximately 100 haploid genomes for rice real-time PCR systems. Real-time PCR cycling conditions of the four assays were stated as standard to facilitate their use in routine laboratory analyses. The assays were finally adapted to conventional PCR for detection purposes, with the exception of the wheat assay, which detects rye simultaneously with similar sensitivity in an agarose gel.


Assuntos
DNA de Plantas/análise , Helianthus/genética , Hordeum/genética , Oryza/genética , Reação em Cadeia da Polimerase/métodos , Triticum/genética , Acetil-CoA Carboxilase/genética , Venenos de Cnidários/genética , Análise de Alimentos , Indústria Alimentícia , Glutens , Proteínas de Plantas/genética , Controle de Qualidade
17.
J Agric Food Chem ; 53(9): 3333-7, 2005 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-15853368

RESUMO

The number of cultured hectares and commercialized genetically modified organisms (GMOs) has increased exponentially in the past 9 years. Governments in many countries have established a policy of labeling all food and feed containing or produced by GMOs. Consequently, versatile, laboratory-transferable GMO detection methods are in increasing demand. Here, we describe a qualitative PCR-based multiplex method for simultaneous detection and identification of four genetically modified maize lines: Bt11, MON810, T25, and GA21. The described system is based on the use of five primers directed to specific sequences in these insertion events. Primers were used in a single optimized multiplex PCR reaction, and sequences of the amplified fragments are reported. The assay allows amplification of the MON810 event from the 35S promoter to the hsp intron yielding a 468 bp amplicon. Amplification of the Bt11 and T25 events from the 35S promoter to the PAT gene yielded two different amplicons of 280 and 177 bp, respectively, whereas amplification of the 5' flanking region of the GA21 gave rise to an amplicon of 72 bp. These fragments are clearly distinguishable in agarose gels and have been reproduced successfully in a different laboratory. Hence, the proposed method comprises a rapid, simple, reliable, and sensitive (down to 0.05%) PCR-based assay, suitable for detection of these four GM maize lines in a single reaction.


Assuntos
DNA de Plantas/análise , Plantas Geneticamente Modificadas/genética , Reação em Cadeia da Polimerase/métodos , Zea mays/genética , Sequência de Bases , DNA de Plantas/química , Dados de Sequência Molecular , Sensibilidade e Especificidade
18.
J Microbiol Methods ; 54(3): 381-90, 2003 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-12842485

RESUMO

The aim of this work was the validation of a rapid, real-time PCR assay based on TaqMan technology for the unequivocal identification of Salmonella spp. to be used directly on an agar-grown colony. A real-time PCR system targeting at the Salmonella spp. invA gene was optimized and validated through a four times repeated blind experiment performed in two different laboratories including 50 Salmonella spp. with representative strains from each of the 5 different Salmonella subgenera and 30 non-Salmonella strains. Both parameters DeltaR(n) (fluorescence intensity of template through a normalized reporter value) and C(T) (cycle at which the fluorescence intensity achieved a pre-established threshold) were analyzed. Overall mean DeltaR(n) and C(T) values for Salmonella strains (2.14+/-0.87 and 15.30+/-0.90, respectively) were statistically different from values for non-Salmonella strains, allowing the establishment of cut-off DeltaR(n) and C(T) values based on 95% confidence intervals that allowed the correct identification of all strains tested in each independent experiment. The accuracy of this assay in terms of inclusivity and exclusivity was 100%. Moreover, the PCR system proved to be especially convenient because the pre-mix containing all PCR reagents except for the bacterial cells could be kept at -20 degrees C for at least 1 month before its use. The optimized TaqMan real-time PCR assay is a useful, simple and rapid method for routine identification of Salmonella spp., irrespective of the particular subgenus.


Assuntos
Reação em Cadeia da Polimerase/métodos , Salmonella enterica/genética , Proteínas de Bactérias/genética , Reprodutibilidade dos Testes , Salmonella enterica/classificação , Sensibilidade e Especificidade , Análise de Sequência de DNA
19.
J Food Prot ; 66(6): 1063-70, 2003 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12801010

RESUMO

In this paper, a method for the specific detection and quantification of potato and tomato DNA in food samples with the use of conventional and real-time polymerase chain reaction (PCR) is described. This method is adequate for use in food quality routine assays involving highly processed samples for which very tiny amounts of DNA are expected. Detection was achieved by amplifying a region of the metallo-carboxypeptidase inhibitor gene from either the potato (PCI) or the tomato (MCPI) and by using specific primers complementary to the propeptide regions of these inhibitors, which were found to differ for the potato and tomato proproteins. Conventional and real-time PCR systems were based on the same potato- or tomato-specific primer pairs, and quantification was carried out with a TaqMan chemistry-based probe. The methods developed proved to be very specific and sensitive and highly reliable for the identification and quantification of DNA from both plant species. In addition, the construction of plasmids pPAT and pTOM, suitable for use as external calibration standards for the elaboration of comparative amplification profiles, is reported. Limits of detection and quantification with the use of these plasmid standards are given. Specificity and copy number conservation among different cultivars were analyzed, and the reliability of these systems was tested through their application to the analysis of commercial food samples including potato and/or tomato as components.


Assuntos
DNA de Plantas/análise , Proteínas de Plantas/genética , Reação em Cadeia da Polimerase/métodos , Solanum lycopersicum/genética , Solanum tuberosum/genética , Sequência de Bases , Calibragem , Contaminação de Alimentos/análise , Manipulação de Alimentos/normas , Solanum lycopersicum/enzimologia , Proteínas de Plantas/isolamento & purificação , Inibidores de Proteases , Padrões de Referência , Sensibilidade e Especificidade , Solanum tuberosum/enzimologia
20.
Cuad. psicol. deporte ; 19(1): 60-75, ene. 2019. tab, graf
Artigo em Inglês | IBECS (Espanha) | ID: ibc-183219

RESUMO

The interactions of a Spanish football team of the Second A (10 official games) are analyzed, evaluating possible behavioral patterns that appear in a regular way in high level football. Observational methodology was used, by Polar Coordinates Analysis, to discover and evaluate the relationships generated between a considered focal behavior and the different conditioned categories, describing behavioral masses among the players. The matches were observed and recorded with an ad hoc observation instrument. The relations of dual character between the players taken as (focal behaviors) right midfielder and forward and the other players (conditioned conducts) were analyzed. The results show differences in the relationship established based on the outcome of the match. Matches that end with a favorable result, the right midfielder takes center stage, as a node of intermediation between the right centre back, left center back, left midfielder, and second striker. In these, the forward is clear receiver in the actions of completion. With the result of the unfavorable match, the connection networks change, generating a network of reciprocal interaction wider and different between the aforementioned player, right midfielder and the rest of the components, with special relation in the players that occupy the right back, left back, right centre back, right winger, left winger, second striker and forward. In these games the striker acquires a role of greater collaboration in the creation in offensive phases, participating as a node in the game network with intermediation functions


Se analizaron las interacciones de un equipo del fútbol español de la Segunda A (Liga 123) (10 partidos oficiales), evaluando posibles patrones de conducta que aparecen de forma regular en el fútbol de alto nivel. Se utilizó metodología observacional, mediante Análisis de Coordenadas Polares, para descubrir y evaluar las relaciones generadas entre una conducta considerada focal y las distintas categorías condicionadas, describiendo mapas conductuales entre los jugadores. Los partidos fueron observados, y registrados con un instrumento de observación ad hoc. Se analizaron las relaciones de carácter dual entre los jugadores tomados como (conductas focales) medio centro derecho y delantero centro y los demás jugadores (conductas condicionadas). Los resultados muestran diferencias en la relación establecida en función del resultado del partido


As interações de uma equipa de futebol espanhol do segundo A (Liga 123) (10 jogos oficiais) foram analisadas, avaliando possíveis padrões de comportamento que aparecem regularmente no futebol de alto nível. Foi utilizada uma metodologia observacional, pela Análise de Coordenadas Polares, para descobrir e avaliar as relações geradas entre um comportamento focal considerado e as diferentes categorias condicionadas, descrevendo mapas comportamentais entre os atores. As partidas foram observadas e registadas com um instrumento de observação ad hoc. Foram analisadas as relações de caráter dual entre os jogadores, tomadas como (comportamentos focais) centro médio direito e centro da frente e os outros jogadores (condutas condicionadas). Os resultados mostram diferenças no relacionamento estabelecido com base no resultado da partida


Assuntos
Humanos , Futebol/psicologia , Comportamento Competitivo , Desempenho Psicomotor , Destreza Motora , Estratégias de Saúde , Processos Grupais , Desempenho Atlético/psicologia , Relações Interpessoais
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